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"THE NECROPSY"
INTRODUCTION:
The word “necropsy” comes from the Greek “nekros dead body + opsis sight”. Necropsy may
be defined as the systematic examination of an animal carcass aimed to search for lesions. It
is an important diagnostic tool and supports other procedures performed in the diagnosis of
disease cases in a herd of flock. There is a literary paradox about the difference between a
necropsy and an autopsy, but the Greek word “Auto” refers to “self” so autopsy is “self
study.” So an autopsy is technically a necropsy, but because a “human is performing it on a
human” it is called an autopsy. The examination of dead or terminally ill animals offers
opportunities in studying the processes involved in disease situations. Although various
medical imaging techniques have evolved in recent years providing adequate information on
the morphologic alterations of organs and tissues following disease; necropsy still provides a
first hand look on what really happened along the course of the disease, particularly in poorly
understood disease situations, tissue alterations resulting from or as a reaction to the disease
process which may or may not be detected during clinical examination. Morphological
changes when correctly recorded and interpreted provide a basis for correlating functional
changes seen in a particular disease process. or functional disturbances. . Morgagni De
Sedibus, 1761 said “Physicians who either performed many autopsies themselves or who
regularly witnessed post mortem examinations, learnt at least to have their doubts. Those,
however, who are not themselves dealing with the very often depressing findings of
autopsy material, are floating in the clouds of uncontrolled optimism”. Ill-performed
necropsy thus confuses the understanding of a disease process. A working routine is desirable
so that adequate information is gathered that will aid in the formulation of a diagnosis. So, a
systematic approach in necropsy is required to so that appropriate and adequate information
be gathered during the examination.
NECROPSY OBJECTIVES:
1. Expose all foci of disease/abnormality in the carcass.
2. Seek lesions to explain clinical and laboratory findings.
3. Identify the sequence of disease events.
i. e. A conscious necropsy investigation includes
1. Systemic observation and dissection.
2. Collection and preservation of appropriate samples (tissue, fluids, etc) for
histologic, cytologic, microbiologic, serologic, chemical, toxicologic, parasitologic,
and/or radiologic evaluation.
3. Record findings logically, accurately, and completely.
4. Interpretation of findings to detect:
a) Immediate cause of death.
b) Contributory causes.
c) Other findings of clinical importance.
d) Incidental findings.
GENERAL CONSIDERATIONS:
TIME:
PLACE:
There are several requirements in the selection of the place for necropsy. The place should
have adequate light, water, ventilation, drainage, provisions for cadaver disposal, and
provisions in lowering the chances of contaminating the surroundings. Animals that died of
suspected transmissible, zoonotic or exotic diseases require that the examination be done in a
laboratory. Usually, a clinical diagnosis will aid in deciding the site for necropsy, for
example, a clinical diagnosis of Anthrax does not warrant necropsy at all for the potential of
contamination is great. Extreme care should be practiced in selecting possible sites for
necropsy, especially in the field. The selected site should be away from sources of feed,
forage and water for the rest of the herd or flock. Avoid those sites that will be frequented by
other animals in the herd in gaining access to other places. Insects, predators and other
biological vectors of diseases should be warded off from the examination site.
NECROPSY EQUIPMENTS:
Usually varies greatly with the species, location of cadaver, etc. But some common
instruments and apparatus must be there for necropsy:
Metallic Instruments:
• Sharp knife and sharpening equip (steel/stone)
• Scalpel
• Tissue forceps and scissors
• Saw, cleaver, osteotome, bone cutter, shears, axe,
• Metric ruler, scale
• Soap, water, brushes for cleaning
• Rubber Boots
• Coveralls/ aprons
• Cut resistant gloves
• Latex or Nitrile gloves
• Sterile gloves
• Duct tape
• Safety glasses or goggles
• Disposable bouffant cap (hair net)
• Surgical or procedural mask
• Lined animal tissue waste containers
• Autoclave
Procedural detail may vary, but a consistent technique aids in a thorough observation. To the
beginner, necropsy techniques appear unreasonably cumbersome and regimented. The
purpose is to methodically expose all organs and tissues to minimize the chance of missing or
"destroying" a lesion. The following is a brief outline of the technique that is to be used when
performing postmortem examinations.
PRENECROPSY EVALUATION:
1. Identify the animal to ensure that the correct animal is being necropsied.
2. Read the clinical history carefully.
3. Examine the necropsy request form for the following:
a. special organs or systems clinicians may want examined.
b. whether or not this is a cosmetic necropsy.
c. special requests (ie, cultures, photos, etc).
4. Fill containers 75% full with 10% phosphate buffered formalin.
5. Label container with necropsy number, species and initials of pathologist on duty.
6. Weigh the animal - if possible.
NB:
# Do not begin the necropsy until a permission sheet signed by the owner is in your
possession; A signed owner’s release form must accompany any animal to be
euthanized.
## Remember that the entire carcass, including all systems and organs, must be carefully
examined. Lesions may appear anywhere and care should be taken to expose and examine all
lesions. Examine each of the paired organs.
### Every animal should be weighed and/or measured (i.e., crown-rump length for aborted
feti) prior to prosection.
NECROPSY EVALUATION
1. EXTERNAL EXAMINATION:
a) Note any abnormal external findings, eg;
• Body Condition: muscle mass / fat stores, decomposition, rigor mortis.
• Skin and hair coat: parasites, dehydration, tumours, wounds, scars.
• Discharges from body orifices: hemorrhage, nasal exudate, diarrhetic feces.
• Eyes: corneal opacities, unequal dilated pupils, exudates, ulcers, hemorrhages.
• Ears: parasites, tumours, discharges.
• Mucous membranes: colour, ulcers.
b) Clinical pathology : Take appropriate samples for culture, histology, cytology, etc.
2. POSITIONING AND OPENING THE BODY CAVITY OF CARCASS:
[may vary from pathologist to pathologist and from animal to animal]
a) Position the animal on its left side down.
b) Reflect the left front and left rear legs.
• To save the cutting edge of knife, insert knife through skin and cut the skin by pulling
out.
• The femoral head should be removed from the acetabulum by cutting the ligament of
the head of femur.
c) Connect the two incisions with an incision along the ventral midline extending from
mandibular symphysis to anus. Do not damage the udder
• To avoid cutting hair, incise the skin from the subcutaneous side
• Raise the front leg and scapula and dissect and reflect dorsally
• Remove the remaining skin between the excised front and rear limb to the
level of the spinal column and reflect dorsally
• Examine the exposed superficial lymph nodes and jugular veins
• Excise through the “up” rear limb (at the level of the pelvis) and continue to
incise through the coxofemoral joint and reflect the rear limb dorsally
d) Reflect skin dorsally.
e) Open abdominal cavity by incising through the dorsal abdominal musculature and
extending your incision downward following the rib cage.
f) Puncture a hole through the diaphragm and listen for air to enter the thoracic cavity (if no
air enters - pneumothorax).
g) Make an incision in diaphragm from the sternum dorsally.
h) Remove the ribs with pruning shears, or a smaller instrument, depending on animal size.
i) Examine viscera. At this time sample organs for microbes (eg bacteria, viruses, parasites)
• Examine both cavities and all contents carefully with minimal movement of the
viscera Note transudates, exudates, and hemorrhage
• Open the pericardial sac
• Note amount, color, and consistency of abnormal fluid accumulations
• Examine for adhesions, displacements, absence of organs, and size and symmetry of
organs in situ
• Record lesions of organs and perform detailed examination of organs prior to removal
• Take initial samples for microbiology, especially exudates in body cavities
Species-Specific Procedures
1. Horse:
• When the abdomen is opened, move the left parts of the large colon cranially so
that the pelvic flexure is lying anterior; move the cecum dorsocranially, the small
Intestine over the right flank, and the small colon posterior and down
• The mucosa of the guttural pouches is examined when the head is disarticulated
• The cranial mesenteric artery should be opened from the aorta past the ilealcecal
and colic artery bifurcations
2. Ruminants:
• When the abdomen is opened, place small intestine and colon over the right lumbar
area; examine the forestomachs and abomasums for position and adhesions
• Remove forestomachs and abomasums as a unit; separate serosal attachments to
stretch the organs out. Open and examine each organ. Remove ingesta and rinse the
rumen mucosa with water to examine.
A. Work within the necropsy room poses many hazards; so all staff must be competent or be
supervised.
B. Immediately prior to beginning necropsy of all large animals (such as cattle, sheep, deer,
etc.), one gallon of household bleach (5% sodium hypochlorite) will be poured into each
of the floor drains.
C. Sharps boxes will be available where required. Used scalpel and razor blades, syringes
and needles will be discarded into sharps boxes and not left on surfaces, especially near
the carcass.
D. Walk carefully around the necropsy room, as the floor may be slippery. Do not rush.
E. Pick up any dropped fat or tissue from the necropsy room floor and keep boots free of
fat/tissue as this increases the risk of staff slipping.
F. Special care will be taken while working with or carrying sharp items, uncovered, around
the necropsy room.
G. When finished using equipment leave any sharp items and other instruments clearly
visible at a predetermined location in the necropsy room.
H. Use of a cart to transport large items around the post mortem room, eg. Bovine head,
sample crates, is recommended.
I. If a staff sustains a cut/injury while working in the necropsy room, they should
immediately take the following action (with assistance if required): Move to a clean part
of the necropsy room. Remove gloves/clothing to reveal and investigate injury.
CUTS: Encourage blood to flow from the cut to flush out the wound. Immerse the cut in
sodium hypochlorite (bleach) at concentrations ≥20,000 ppm for 1 minute, then wash and
dry the cut area. Apply direct pressure to limit bleeding if necessary.
OTHER INJURIES: All contaminated outer clothing must be removed before staff leave
the post-mortem room – unless to do so would endanger life or risk further injury.
For species specific necropsy procedures, the following
references are recommended:
a. Feldman, D. B. and Seely J.C., Necropsy Guide: Rodents and the Rabbit. CRC
Press Inc., Boca Raton Fl, 1988.
b. Pathology of Domestic Animals 3rd ed., vols. 1-3 Academic Press Inc., 1985.
c. Devor, D.E., Henneman, J.R., Kurata, Y., et al. Pathology Procedures in
Laboratory Animal Carcinogenesis Studies. In Waalkes, M.P. and Ward, J.M.
(eds.), Carcinogenesis, New York: Raven Press, pp. 429-466, 1994. d.
Sundberg, J.P. and Boggess, B. Systematic Approach to Evaluation of
Mouse Mutations. Boca Raton: CRC Press p. 199, 1999.
REFERENCES
Benjamin, L.E. 1985. Veterinary clinical pathology. Kallyani publication, new delhi.
Calnek, B.W.1997. Diseases of poultry. 10th Edition lowa state University press, Ames, Lowa
GEERING WA, FORMAN AJ, NUNN MJ, Exotic Diseases of Animals, Aust Gov
Publishing Service, Canberra, 1995, p.173-181
Luna, L.G. 1968. Histopathologic Technic and Practical Histochemistry, 3rd ed. Sounders
Co.ltd, London.pp.17-120.
NADC Guidelines for accidental personal injury
NADC Procedures for Incineration and Operation of the Necropsy Facility. (Sept. 2004)
Veterinary necropsy protocol for milliatary working dogs and pathological sample
submission guidelines. 2001, Headquarters, department of US army.
WHO Infection Control Guidelines for Transmissible Spongiform Encephalopathies. 1999