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osirix-viewer.com/Snapshots.html
resource
by Tonya Limberg © 2008, MS, Biomedical Visualization, University of Illinois at Chicago
Introduction
Osirix is a freeware program available to the public ences learning this program and what I found useful
on the Apple Inc. Website. Biomedical Visualizers can for Biomedical Visualizers.
use this software to visualize anatomical data sets and
extract visual information for reference. I have included Terms you can use throughout the
tutorial and there is also a list of terms at the end of the
This tutorial is directed to the novice user for visualiza- tutorial for your reference.
tion needs. I found that the resources I came across for
Osirix were primarily for the radiologist or computer Any questions or comments can be directed to tlim
programer. I will share with you my personal experi- berg@wi.rr.com. I hope you find this tutorial useful.
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Table of Contents
Getting Started............................................................. 3 Export 3D................................................................... 15
Navigation.............................................................. 4-11 Technique............................................................. 16-20
Sources for Data......................................................... 12 Resources.................................................................... 21
File Formats................................................................ 13 Index..................................................................... 22-23
Export Images............................................................ 14
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Terms you can use
DICOM
File extension – .dcm
Digital Imaging and Com-
munications in Medicine.
CT
Computed Tomography
Image sections are created
from a 2D Xray moving
www.apple.com/downloads/macosx/imaging_3d/osirix.html pubimage.hcuge.ch:8080/ around a single axis. They
form a 3D image of inter-
nal anatomy.
MRI
Magnetic Resonance
Imaging
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Toolbar
Thumbnails CD-Rom
import
from cd
Export
export data
Preview
Anonymize
remove ID
iPod
export to iPod
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Osirixas a
Toolbar resource
TOOLS you can use
Database
return to window
Thumbnails
Tile
tile view
Series
previous/next
Patient
Preview previous/next
Contrast
Adjustment
contrast & intensity
Move and Pan
command key
Zoom
Osirix shift key
Rotate
horizontally move
Length
The Viewer window displays data sets from a select when using the Contrast and Adjustment tool. measure distance
series and allows you to manipulate 2D images. The Contrast is also referred to as Window Length (WL)
Viewer window has a main toolbar, image thumbnails and intensity as Window Width (WW). The tool WL
WL & WW
and a preview pane. If you click on the Database tool & WW has some preset contrast and intensity settings preset contrast and intensity
the Viewer window will close. in the pulldown menu.
CLUT
Move the mouse in a horizontal direction to change The CLUT (Color Look Up Tables) tool is used to as- Color Look Up Tables
assign color
contrast and a vertical direction to change intensity sign color to the images.
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Osirixas a
Toolbar
resource
TOOLS you can use
Thumbnails
Orientation
plane of reference
Thick Slab
Preview change slab thickness
Movie Export
create a
Quicktime movie
Browse
display cine
images
Osirix
Rate
speed of browse button
Two export features in the viewer window are Movie The Thick Slab tool increases the original thin slabs to
Print
Export and Print. The rest of the tools featured on this a thicker viewing to increase viewing depth. print image
page use 3D representation in the 2D viewer window.
Browse gives a dynamic display of static images. The Sync
The Orientation tool allows the flexibility of chang- rate at which Browse moves through the stack is de- applies changes
ing between axial, coronal and sagittal views in the termined by moving the Rate slider to the right of the to all images
preview window. The 3D tool displays data in 3D in a Browse tool. Propagate
separate window. ROI to all
images in stack
Propagate and Sync apply changes throughout the stack.
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Osirixas a
2D/3D resource
TOOLS you can use
2D/3D
open 3D
window
Osirix
osirix-viewer.com/Snapshots.html
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Osirixas a
2D/3D resource
Terms you can use
MIP
Maximum Intensity Projection
high contrast for 3D object
Contrast (vasculature)
osirix-viewer.com/Snapshots.html
Osirix
osirix-viewer.com/Snapshots.html
Rotate
Preview horizontally move
Manipulate
around center
of gravity
Angle of View
camera position
Length
measure distance
Osirix
Tag Reference Points
specific feature
The third window appears once a selection has been Preset opacities are available in the Opacity tool. The Bone Removal
made from the 2D/3D viewer button pulldown menu. Manipulate tool moves the object around it’s center of removes bone
This windows is titled according to the selection chosen. gravity. The camera position can be changed with the
preset contrast and intensity
The 3D window has a main toolbar and preview pane. Angle of View tool. A Tag Reference Point tool is used
to point out a specific feature. The Sculpt 3D Object
Color Look Up Tables
The Contrast, Move and Pan, Zoom, Rotate, Length, tool acts as a scissors. Material is outlined by clicking assign color
WL & WW and CLUT tools are the same as in the points to form a shape to be deleted. Click on a bone
Viewer window. Note that color can be manipulated in with the Bone Removal tool, and it will remove it. De- Opacity preset opacities
the histogram when making a bit selection at the bot- tail can be adjusted in the Fine /Course slider. Detail
tom of the CLUT pull-down menu. fine
course
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Toolbar resource
TOOLS you can use
Best Rendering
resolution
Crop Volume
frame to crop
Orientation
toggles box in
Preview corner on/off
DICOM file
export DICOM
Fly Thru
animated movie
Osirix
Engine
Ray cast
Navigation
3D texture
Window 3 3D Volume Rendering
Other tools for the 3D Volume Rendering window The DICOM file tool exports DICOM files.
include the Best Rendering tool. This tool renders the Perspective
image at it’s best resolution. An animated movie can be created with the Fly Thru advanced
tool that can be inserted into Microsoft PowerPoint.
The Crop Volume tool has a wire frame that creates a
box around the object to crop it. The Orientation tool Ray cast and 3D texture can be applied with the
toggles the visibility of the orientation box in the up- Engine tool pulldown menu. Advanced tools in Per-
per right corner of the preview window. spective and Shading are also available. Shading advanced
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data websites
pubimage.hcuge.ch:8080/x
sourceforge.net/proj
ect/showfiles.php?group_
id=107249&package_
id=165209&release_
id=360395
sourceforge.net/project/showfiles.php?group_ www.nlm.nih.gov/re
id=107249&package_id=165209&release_
id=360395
search/visible/visible_gal
lery.html
pubimage.hcuge.ch:8080/
www.barre.nom.fr/
medical/samples/
ftp://ftp.erl.wustl.edu/pub/
www.barre.nom.fr/medical/samples/
dicom/
ftp://ftp.nlm.nih.gov/vis
nlm.nih.gov/research/visible/visible_gallery.html
ible/bitmaps/color24/
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DICOM
JPEG TIFF
Terms you can use
TIFF (.tiff)
Tagged Image File Format.
AVI
Stores image data in tags.
JPEG (.jpg)
Quicktime
Joint Photographic
MPEG Experts Group.
Lossy compression.
12-bit jpeg for medical
systems
PDF (.pdf)
Portable Document Format
Independent of software,
AVI (.avi)
Osirix accepts many file formats. These file formats Format is independent of software, hardware or OS. It Audio Video Interleave.
include DICOM, TIFF, JPEG, PDF, AVI, MPEG and can contain text, fonts & images. AVI (.avi) or Audio Audio & video data.
Quicktime. Video Interleave contains audio & video data. The
MPEG (.mpg) or Moving Picture Experts Group con- MPEG (.mpg)
TIFF (.tiff) or Tagged Image File Format stores image tains video & audio data and has various compression Moving Picture
Experts Group.
data in tags. It does not lose file data when it is com- formats. Quicktime (.mov) is a video, media, sound, Video & audio data.
pressed and therefore is referred to as lossless com- text, animation and image interactive file format. Compression formats.
pression. The TIFF has advanced pixel data types for
scientific imaging. JPEG (.jpg) or Joint Photographic The file formats were tested with Osirix and all import- Quicktime (.mov)
Experts Group uses lossy compression and may lose ed to confirm compatibility. No file formats have been Video, media, sound text,
animation, interactive
data if saved multiple times. There is a 12-bit jpeg for found at this time that need to be converted.
medical systems. PDF (.pdf) or Portable Document
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TOOLS you can use
Export
export data
iPod
export to iPod
Burn
burn cd
Images can be exported from Osirix by numerous Images can be exported to an iPod to be stored or File Menu – Export
means. viewed. To export images to store, connect the iPod format selection
to the computer and click on the iPod tool. To export
DICOM images can be exported with the Export tool. images to view, select export under the file menu and
select iPhoto. Create an album in iPhoto for Osirix
Selecting Export under the File menu allows you to images then open iTunes. Select the iPod and click on
export to Quicktime, jpeg, raw, tiff, DICOM, email and the Images tab. You can select the Osirix folder to be
iphoto. viewed. In the future you will be able to store Osirix
images on your iPhone.
The Burn tool burns files to a CD with the Osirix
viewer included so others can view the files. I would recommend storing files on a CD or iPod to re-
duce the amount of space taken up on your hard drive.
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TOOLS you can use
DICOM File
export DICOM
Movie Export
create a
Quicktime movie
VR Movie Export
create interactive
Quicktime movie
Export 3D
DICOM, Quicktime movies, Quicktime VR movies sequence. Click on the fly thru tool and a dialogue box
and Fly Thrus can be exported in the 3D window. allows you to add or delete images by clicking on the
Plus or Minus signs. Change the angle of the object,
Click on the DICOM tool to export a new set of DI- zoom in and out, and affect the contrast and intensity
COM images to a desired location. levels while adding these to the fly thru. In order to
view the movie click on the Movie tab in the dialogue
The Quicktime tool generates a 180 or 360 degree box. Click on Compute and under the Frame section
movie. The Quicktime VR tool creates an interactive play the movie. If the movie jumps, try adding extra
movie that you can rotate in real time. transition images when creating something like an
intense zoom. To export the movie click Save in the
The Fly Thru tool allows you to create a dynamic image Movie tab to export a Quicktime movie.
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Osirixas a
On/Off
resource
Terms you can use
Select
Angiogram
mask image Static or dynamic.
Used to visualize the lumen
of vessels or organs.
Before subtraction After subtraction Contrast agent injected
shows up on x-ray.
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Terms you can use
ROI
Region Of Interest
emphasized area on image
Pulldown menu
other ROI tools
Osirix
The Repulsor tool manipulates the ROI already cre- Region Growing allows you to select a point and it will
ated, and the Selector tool makes multiple selections. grow the area to include similar pixel density ranges.
By double clicking on any region of interest a dialogue
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TOOLS YOU CAN USE
Grow Region
similar pixel densities
added to pixel selected
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Osirixas a
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TERMS YOU CAN USE
Ray-trace
simulates the effects of light
shading, lighting, color and
transparency
Renderman (.rib)
RenderMan
interface between rendering
and modeling programs
VRML (.vrml)
Virtual Reality Modeling
Language
represent 3D vector graphics
Inventor (.iv)
code to display 3D objects
Osirix two surface renderings Osirix one surface rendering
Wavefront (.obj)
3D object file
STL (.stl)
representing solid models
After selecting 3D Surface rendering a dialogue box A Ray-trace rendering effect is generated on the sur-
appears. This box has Predefined Pixel Values for the face using these parameters to apply shading, lighting,
first and second surfaces. A transparency can be added color and transparency to the object.
to visualize the surface beneath. The Resolution and
Smooth Iterations can be adjusted with trial and error A 3D surface model can be exported that is compatible
to better represent the form of the object that is being with graphic modeling programs such as 3D Studio
created with triangles on it’s surface. Max and Maya. The Export 3D-SR tool exports five Dialogue Box 3D Surface
file formats. They are Renderman, VRML, Inventor, define pixel value
resolution and iterations
Wavefront and STL.
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TOOLS YOU CAN USE
4D Viewer
sync PET and
CT scans
4D Player
Plays dynamic sequence
Select all of the image thumbnails in the Database Customize the toolbar so the 4D Player tool is in the
window and click on the 4D tool. toolbar. This 4D player tool has a Play button to acti-
vate the beating heart.
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Wikipedia
http://en.wikibooks.org/
wiki/Online_Osirix_Docu
mentation
Resources
This tutorial was produced to improve the novice users By providing terminology, navigation and techniques
understanding of Osirix as a resource. that pertain to the Biomedical Visualization user, I Help Menu in Osirix
hope to promote the use of Osirix as a resource.
Other resources that were used in creating this tutorial
are listed in the sidebar to the right for your reference.
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Osirixas a
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Terms you can use
3D window (p. 4) DICOM (p. 3) MPEG (.mpg) (p. 13) PDF (.pdf) (p. 13) ROI (p. 17)
view and manipulate File extension – .dcm Moving Picture Portable Document Format Region Of Interest
3D data sets Digital Imaging and Commu- Experts Group. Independent of software, hard- emphasized area on image
nications in Medicine. Video & audio data. ware or OS.
The DICOM file standard is Compression formats. Text, fonts & images STL (.stl) (p. 19)
Angiogram (p. 16)
used in image and patient data MPR (p. 8) representing solid models
Static or dynamic.
Used to visualize the lumen of from CT, MRI and PET medi- Multiplanar Reformatting PET (p. 3)
vessels or organs. cal imaging equipment. slicing through data in a new way Positron Emission TIFF (.tiff) (p. 13)
Contrast agent injected shows Tomography Tagged Image File Format.
up on x-ray. MRI (p. 3) 3D image of metabolic activity Stores image data in tags.
Dynamic gated cardiac (p. 20)
continuous portrayal of the Magnetic Resonance is created using gamma rays Lossless compression.
AVI (.avi) (p. 13) heart over time Imaging that interact with a metaboli- Advanced pixel data types for
Audio Video Interleave. Magnetic field is created cally active molecule. scientific imaging.
Audio & video data. Inventor (.iv) (p. 19) around anatomical structures
code to display 3D objects creating an image. Quicktime (.mov) (p. 13) Viewer window (p. 4)
CT (p. 3) Video, media, sound text, ani- view and manipulate
Computed Tomography JPEG (.jpg) (p. 13) PACS (p. 5) mation, interactive 2D data sets
Image sections are created Joint Photographic Picture Archiving &
from a 2D Xray moving around Experts Group. Communication System Ray-trace (p. 19) VRML (.vrml) (p. 19)
a single axis. They form a 3D Used to coordinate simulates the effects of light Virtual Reality Modeling
Lossy compression.
image of internal anatomy. workflow of scans shading, lighting, color and Language
12-bit jpeg for medical systems represent 3D vector graphics
transparency
Database window (p. 4) MIP (p. 9)
Renderman (.rib) (p. 19) Wavefront (.obj) (p. 19)
import data sets (com+D) Maximum
RenderMan 3D object file
Intensity Projection interface between rendering
high contrast for 3D object
and modeling programs
Index
This list of terms is offered to aid in your Page numbers are given for each term. Look for and information on the discussion topic.
understanding of Osirix. sidebars on the accompanying pages for terms
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Osirixas a
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TOOLs you can use
2D-3D Viewer (p. 5) 3D Surface Rendering Browse (p. 7) Detail (p. 10) iPod (p. 5, 14) Perspective (p. 11) Subtraction mask
open viewer window (p. 9) display cine fine/course export to iPod advanced (p. 16)
rendering volume for images Separate dynamic
2D/3D (p. 8) 3D programs Dialogue box 3D Import (p. 5) Print (p. 7) portion of image
open 3D window Burn (p. 5, 14) Surface (p. 19) import data print image from the rest.
3D Volume Rendering burn cd define pixel value
2D MPR (p. 8) (p. 9, 20) resolution and Length (p. 6, 10) Propagate (p. 7, 17) Sync (p. 7)
tissue intensity values CD-Rom (p. 5) iterations applies changes to
oblique planes across measure distance ROI to all
data import images in stack all images
4D Player (p. 20) from cd DICOM file (p. 11, 15)
Manipulate (p. 10)
Plays dynamic sequence export DICOM Tag reference points
2D Curved MPR (p. 8) around center of gravity Rate (p. 7)
curved planes across CLUT (p. 6, 10) speed of browse button (p. 10)
4D Viewer (p. 5, 20) Color Look Up Tables Engine (p. 11) specific feature
data Move and Pan (p. 6, 10)
sync PET and CT scans assign color Ray cast 3D texture
command key Rotate (p. 6, 10)
2D Orthagonal MPR horizontally move Thick slab (p. 7)
Albums & Sources Contrast (p. 6, 10, 18) Export (p. 5, 14) change slab thickness
(p. 8) Movie export
(p. 5) Adjustment export data
axial, coronal, sagittal (p. 7, 15) Scroll (p. 6)
planes create data sets contrast & intensity create a through image set Tile (p. 6) tile view
File menu – export
Quicktime movie
Angle of View (p. 10) Convolution filters (p. 14) VR Movie export
3D Endoscopy (p. 9) Sculpt 3D object
camera position (p. 16) format selection (p. 15)
navigate through lumen Opacity (p. 10) (p. 10, 18)
Enhance image for preset opacities scissors cut out create interactive
Anonymize (p. 5) further separation. Fly Thru (p. 11, 15) Quicktime movie
3D MIP (p. 9)
remove ID animated movie
Maximum Intensity Orientation (p. 7, 11) Series (p. 6)
Projection Crop volume plane of reference previous/next WL & WW (p. 6, 10)
Grow Region preset contrast and
Contrast (vasculature) Best rendering (p. 11) (p. 11, 18) intensity
resolution frame to crop (p. 17, 18)
Patient (p. 6) Shading (p. 11)
similar pixel densities
3D panel (p. 7) previous/next advanced Zoom (p. 6, 10)
Bone removal (p. 10) Database (p. 6) added to pixel selected
data in 3D shift key
removes bone return to window
Index
This list of tools is offered to aid in your un- Page numbers are given for each tool. Look tools and information on the discussion topic.
derstanding of Osirix. for sidebars on the accompanying pages for
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