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METABOLISM
B. Many intermediate products are produced between starting reaction and end
products.
3. Metabolic Pathways
A. Photosynthesis
1. Occurs in cells that produce chlorophyll
2. Catalyst: light-activates chlorophyll
3. Energy: produced when electron released by chlorophyll
light
4. 6CO2 + 12H2O →→→→→→→→C6H12O6 + 6H2O + 6O2
chlorophyll→e- (glucose)
5. Phototrophs :
a. Photoautotrophs - utilize inorganic compounds (i.e., CO2)
b. Photoheterotrophs - utilize organic compounds (i.e., methanol)
B. Chemosynthesis
1. Occurs in cells that do not produce chlorophyll.
2. Energy: produced by oxidation of chemical compounds (i.e., sugars).
3. Catalyst: enzymes produced by cell.
4. Chemotrophs :
a. Chemoautotrophs - utilize inorganic compounds (i.e., CO2)
b. Chemoheterotrophs - utilize organic compounds (i.e., glucose)
1
ENZYMES
1. Importance
A. Determines biochemical activity (metabolic reactions).
B. Inherited characteristic so biochemical activity used for identification
classification, classification.
C. Chemical agents, antibiotics often interfere with enzyme activity to inhibit
bacterial growth.
D. Responsible for many pathological changes in host tissue.
E. Industrial application dependent on enzyme activity of bacterial cell.
F. Environmental activity dependent on enzymes produced by bacterial cell.
2. Characteristics
A. Large globular proteins.
B. Heat sensitive - 10° doubles activity; easily denatured by excess heat
C. pH sensitive - easily denatured by acid, alkaline conditions.
D. Organic catalysts - speed up reactions by lowering activation energy.
E. Specific in action - reacts with one particular substrate,
catalyze one type reaction.
F. Specificity is determined by active site on enzyme.
that is complementary to combining site on substrate.
G. Do not directly enter reaction, remain unchanged, so can react with another
molecule of same substrate.
3. Coenzymes, Cofactors
A. Coenzymes
1. Organic molecules - vitamins
2. "Carrier" molecules - transfer electrons from one molecule to another.
B. Cofactors
1. Inorganic molecules - metal ions
2. Completes structure of active site on enzyme so that it is
complementary to combining site on substrate.
2
PRODUCTION OF ENERGY
3
EMBDEN MEYERHOFF
GLUCOSE (6 CARBONS)
(DEXTROSE)
↓
↓
2 ATP→→→→2 ADP + E
↓
↓
2 3-CARBON COMPOUNDS
↓
↓
2 H+ →→→→2 H + CoEz
↓
4 ADP →→→→4 ATP
↓
↓
2 PYRUVIC ACID (3 CARBONS)
↓ ↓
↓ ↓
FERMENTATION ACETYL CO A
(ANAEROBIC) ↓ → 2 H+COEZ
↓ RESPIRATION
↓ (AEROBIC)
↓ ↓
ORGANIC ACIDS KREBS CYCLE
ORGANIC ALCOHOLS
2 ADP → 2 ATP
→
→→
H+ H+COEZ
↓
ELECTRON
TRANSPORT
↓
↓
↓
CO2 + H2O
34 ATP