GC Column Selection Chart

Polarity
Scale
Composition Column Profile Applications Specifications Benefits
The polarity scale is based on their McReynolds value, which provides a systematic approach to ranking GC stationary phases by polarity. Low High

5
Polarity Scale

ZB-1 CH3
Si O
Polarity
Bleed
• Essential oils
• Gases (refinery)
• Hydrocarbons
• Oxygenates and GROs
• Semi-volatiles
• Simulated distillation
Temperature limits -60 to 360/370 °C (isothermal/TPGC)*
USP phase G2
• Low activity
• High efficiency
Non-polar phase suited for true Temperature Limits *Thicker films (≥1.0 µm df) are rated to 340/360 °C (isothermal/TPGC). • Excellent resolving power of critical pairs in complex petrochemical samples
• MTBE • Sulfur compounds (light)
boiling point separations CH3 Stability • Natural gas odorants • Solvent impurities • Low bleed (MS certified)

100 %
Dimethylpolysiloxane

5 ZB-1ms
Extremely low bleed column
for non-polar compounds
CH3
Si
CH3
O
Polarity
Bleed
Temperature Limits
• Drugs of abuse
• Amines
• Acids
• Polychlorinated biphenyls
(EPA Method 1668)
• Flavors & fragrances
• Diesel fuel
• Pesticides
Temperature limits -60 to 360/370 °C (isothermal/TPGC)*
USP phase G2
Available with Guardian™ integrated guard columns
• Lowered bleed (MS certified) especially suited to high sensitivity work using
GC/MS
• Extremely inert for active compounds such as drugs or pesticides
• Improved signal-to-noise ratio for better sensitivity and mass spectral
Stability
integrity

100 % • Identical selectivity to ZB-1

Dimethylpolysiloxane

5 ZB-1HT
INFERNO ™
CH3
Si
CH3
O
Polarity
Bleed
Temperature Limits
• High boiling petroleum
products
• Simulated distillation
methods
• Polymers/Plastics
• High molecular weight waxes
• Diesel fuel
• Motor oils
Temperature limits -60 to 400/430 °C (isothermal/TPGC)*
USP phase G2
Available with Guardian™ integrated guard columns
*0.53 mm ID columns are rated to 400 °C max operational temperature
• Non-metal 100 % dimethylpolysiloxane phase stable to 430 °C
• Rugged high temperature polyimide coated, fused silica tubing
• Provides true boiling point separation for hydrocarbon distillation methods
Stability • Long-chained hydrocarbons • Provides robust column performance for high temperature bake outs
High temperature stability (430 °C)
for non-polar compounds
2007
100 %
WINNER Dimethylpolysiloxane

8 ZB-5
Versatile low polarity column for
unknown samples
CH3
Si
CH3
O Si O
Polarity
Bleed
Temperature Limits
• Alkaloids
• Drugs
• FAMEs
• Halo-hydrocarbons
• Residual solvents
• PCBs/Aroclors
• Essential oils/Flavors
• Semi-volatiles
Temperature limits -60 to 360/370 °C (isothermal/TPGC)*
USP phase G27
Available with Guardian™ integrated guard columns
*Thicker films (≥1.0 µm df) are rated to 340/360 °C (isothermal/TPGC).
• Low bleed (MS certified) especially suited to high sensitivity work using
GC/MS
• Extremely inert for active compounds such as drugs or pesticides
• Resilient to dirty samples – long column life
Stability • Phenols • Dioxins
• Pesticides/Herbicides • Great column for unknown samples
95 % 5%
Dimethylpolysiloxane Phenyl

8 ZB-5ms
Arylene phase for enhanced resolution of
PAHs and multi-ring aromatic compounds
CH3
Si
CH3
O
CH3
Si O
CH3
Polarity
Bleed
Temperature Limits
• Drugs
• Amines
• Alkaloids
• FAMEs
• Polyaromatic
hydrocarbons (PAHs)
• Polychlorinated biphenyls
(PCBs)/Aroclors
Temperature limits -60 to 325/350 °C (isothermal/TPGC)
USP phase G27
Available with Guardian™ integrated guard columns
• Low bleed (MS certified) especially suited to high sensitivity work using
GC/MS
• Extremely inert for active compounds such as drugs or pesticides
• Fully conditioned within 35 minutes
Stability • Halo-hydrocarbons • Acids
• Dioxins • Pesticides/Herbicides • Enhanced resolution of PAHs and other multi-ring aromatic compounds
95 % 5%
Dimethylpolysiloxane Phenyl-Arylene

8 ZB-5MSi
Low-bleed column with the
same selectivity as the ZB-5
CH3
Si
CH3
O Si O
Polarity
Bleed
Temperature Limits
Stability
• Drugs of abuse
• Nitrosamines
• Phenols
• FAMEs
• Pesticides
• EPA Methods
Temperature limits -60 to 360/370 °C (isothermal/TPGC)
USP phase G27
Available with Guardian™ integrated guard columns
• Highly inert – improved peak shape for acidic/basic compounds
• MS certified low-bleed levels provide maximum sensitivity
• Industry leading QC specifications ensure column-to-column performance
• Traditional bonding chemistry provides the same selectivity as the ZB-5
columns
95 % 5%
Dimethylpolysiloxane Phenyl

8 ZB-5HT
INFERNO ™
CH3
Si
CH3
O Si O
Polarity
Bleed
Temperature Limits
• High boiling petroleum
products
• Simulated distillation
methods
• Triglycerides
• Diesel fuel
• Motor oils
• Surfactants
Temperature limits -60 to 400/430 °C (isothermal/TPGC)*
USP phase G27
Available with Guardian™ integrated guard columns
*0.53 mm ID columns are rated to 400 °C max operational temperature
• Non-metal 5 % - phenyl - 95 % - dimethylpolysiloxane phase stable to
430 °C
• Rugged high temperature, polyimide coated, fused silica tubing
• Provides true boiling point separation for hydrocarbon distillation methods
Polarity Scale

Stability • Polymers/Plastics • Long-chained hydrocarbons

High temperature stability (430 °C) • High molecular weight waxes • Provides robust column performance for high temperature bake outs
for high boiling compounds
95 % 5%
2007
WINNER Dimethylpolysiloxane Phenyl

9 ZB-XLB
Low polarity si-arylene column with bleed and
sensitivity levels designed for MS detectors
Proprietary
Polarity
Bleed
Temperature Limits
Stability
• Polychlorinated Biphenyls (PCBs)
• Pesticides
• Herbicides
Temperature limits - 60 to 340/360 °C (isothermal/TPGC)*
Available with Guardian™ integrated guard columns
*Thicker films (≥1.0 µm df) are rated to 320/340 °C (isothermal/TPGC).
• Unique low polarity si-arylene column for MS detectors
• Provides alternative selectivity to standard 5-type phases
• Often used for confirmation of pesticide, PCB, or other environmental samples
• Good tool for sample screening to identify unknown contaminants

9 ZB-XLB-HT
Inferno ™ Proprietary
Polarity
Bleed
Temperature Limits
Stability
• Polychlorinated Biphenyls (PCBs)
• Pesticides
• Herbicides
• EPA Methods
Temperature limits - 60 to 400 °C (isothermal/TPGC)
Available with Guardian™ integrated guard columns
• Non-metal si-arylene GC column stable to 400 ˚C
• Good tool for general screening to identify unknown samples
• Individually tested for low bleed, MS certified

Enhanced durability with

400 ˚C temperature stability

11 ZB-MultiResidue -1
Novel phase designed for pesticides,
herbicides, and insecticides
™

Proprietary
Polarity
Bleed
Temperature Limits
Stability
• Organochlorine pesticides
• Organophosphorus
pesticides
• Nitrogen containing
• Insecticides
• Aroclors/PCBs
• Multi-pesticide residue
methods
Temperature limits - 60 to 320/340 °C (isothermal/TPGC)
EPA Method 8081A, 8141, 8151 Certified
• Designed for the separation of all types of pesticides, herbicides, and
insecticides
• Provides baseline resolution and confirmation of all 20 chlorinated pesticides
regulated under EPA Method 8081 in ≤10 min
pesticides • Haloacetic acids
• Herbicides • MS Certified phase provides low bleed performance for pesticide confirmation
by MS
~exclusive~ • Low activity, decreased breakdown of sensitive pesticides such as DDT

13 ZB-624
Optimized for separating volatile
organic compounds (VOCs)
CH3
Si
CH3
O
C N
(CH2 )3
Si O
Polarity
Bleed
Temperature Limits
Stability
• Volatile organic
compounds (VOCs)
• Residual solvents in
pharmaceutical products
• US EPA Methods 501.3,
502.2, 503.1 524.2, 601,
602, 624, 8010, 8015,
8020, 8021, 8240, 8260
Temperature limits -20 to 260 °C
USP phase G43
Available with Guardian™ integrated guard columns
• Formulated for low bleed
• Increased temperature limit speeds run times and re-equilibration
• Available in Fast GC dimensions
(OVIs) • Separate volatile organic flavor and fragrance additives and residual solvents
in industrial or pharmaceutical products (OVIs)
94 % 6%
Dimethylpolysiloxane Cyanopropylphenyl

15 ZB-MultiResidue -2
Novel phase designed for pesticides,
herbicides, and insecticides
™

Proprietary
Polarity
Bleed
Temperature Limits
Stability
• Organochlorine pesticides
• Organophosphorus
pesticides
• Nitrogen containing
• Insecticides
• Aroclors/PCBs
• Multi-pesticide residue
methods
Temperature limits - 60 to 320/340 °C (isothermal/TPGC)
EPA Method 8081A, 8141, 8151 Certified
• Designed for the separation of all types of pesticides, herbicides, and
insecticides
• Provides baseline resolution and confirmation of all 20 chlorinated pesticides
regulated under EPA Method 8081 in ≤10 min
pesticides • Haloacetic acids
• Herbicides • MS Certified phase provides low bleed performance for pesticide confirmation
by MS
~exclusive~ • Low activity, decreased breakdown of sensitive pesticides such as DDT

18 ZB-35
Intermediate polarity column for high
molecular weight
CH3
Si
CH3
O Si O
Polarity
Bleed
Temperature Limits
Stability
• Pesticides
• Aroclors
• Drugs of abuse
• Amines
• Semi-volatiles
• Steroids
Temperature limits 50 to 340/360 °C (isothermal/TPGC)
USP phase G42
• Intermediate polarity column for high molecular weight analysis
• Inert to minimize analyte adsorption, improve efficiency, and reproducibility
• More rugged (longer column life) than other polar phases
• Often resolves compounds that co-elute on ZB-1 or ZB-5 phases

65 % 35 %
Dimethylpolysiloxane Phenyl

18
Polarity Scale

ZB-35HT CH3 Polarity

• Aroclors
• Semi-volatiles
• Pesticides
• Drugs of abuse
Temperature limits -60 to 400 °C (isothermal/TPGC) • First non-metal, 35 %-Phenyl columns stable to 400 ˚C
• Great for high molecular weight compounds
Inferno ™ Si O Si O Bleed • Amines • Pharmaceuticals USP phase G42
Temperature Limits • EPA Methods 508, 608, • Steroids • Individually tested for low bleed, MS certified
CH3 Stability 8081, 8141, 8151 • Chemicals
Enhanced durability witih
400 ˚C temperature stability
65 % 35 %
Dimethylpolysiloxane Phenyl

19 ZB-1701P
Specially tested to ensure good
response for DDT and endrin
CH3
Si
CH3
O
C N
(CH2 )3
Si O
Polarity
Bleed
Temperature Limits
Stability
• Organochlorine pesticides • Nitrogen containing
• Aroclors
• Organophosphorus
pesticides
pesticides
Temperature limits -20 to 280/300 °C (isothermal/TPGC)
EPA Method 8081A Certified
*Thicker films (≥ 1.0 µm df) are rated to 260/280 °C (isothermal/TPGC).
• Specially tested to ensure good response for DDT and endrin
• Guaranteed column for pesticide analysis

Note: Consider using ZB-MultiResidue 1 and 2 as a more rugged alternative for pesticide analysis.

86 % 14 %
Dimethylpolysiloxane Cyanopropylphenyl

24 ZB-50
High polarity column capable of high
temperature bake-out to remove
CH3
Si
CH3
O Si O
Polarity
Bleed
Temperature Limits
Stability
• Drugs of abuse
• Pesticides/Herbicides
• Aroclors
• Antidepressants
• Cholesterols
• Triglycerides
• Steroids
• Glycols
Temperature limits 40 to 320/340 °C (isothermal/TPGC)
USP phase G3
• High polarity column capable of high temperature bake-out to remove
contaminants
• Inert to minimize analyte adsorption, improve efficiency, and reproducibility
• More rugged (longer column life) than other polar phases
contaminants
• Great for drug screening and environmental compounds
50 % 50 %
Dimethylpolysiloxane Phenyl

52 ZB-WAXPLUS
100 % aqueous stable with high retention of
alcohols and chlorinated solvents
™ H H
O C C O
H H
Polarity
Bleed
Temperature Limits
• Alcohols
• Aldehydes
• Aromatics
• Essential oils
• Pharmaceuticals
• OVIs
• Solvents
• Styrene
Temperature limits 20 to 250/260 °C (isothermal/TPGC)
USP phase G16
• 100 % aqueous stable
• Extremely inert for acidic compounds
• Enhanced selectivity for low boiling solvents
Stability • Flavors & fragrances • Xylene isomers • High retention of alcohols and chlorinated solvents
• Glycols • Alcoholic beverages
100 %
Polyethylene Glycol

57 ZB-WAX
Bonded, solvent rinsable phase excellent
for separating polar complex mixtures
H H
O C C O
H H
Polarity
Bleed
Temperature Limits
Stability
• Alcohols
• Aldehydes
• Aromatics
• Essential oils
• Pharmaceuticals
• Solvents
• Styrene
• Xylene
Temperature limits 40 to 250/260 °C (isothermal/TPGC)
USP phase G16
• Low bleed; MS certified
• Highly stable, long lifetime
• Low activity for amines
• Flavors & fragrances • Basic compounds • Excellent chromatography of polar complex mixtures
• Glycols
100 %
Polyethylene Glycol

58 ZB-FFAp
Provides better peak shape
for underivatized acids
H H
O C C O
H H
Polarity
Bleed
Temperature Limits
• Acrylates
• Alcohols
• Aldehydes
• Flavor mixtures
• Ketones
• Organic acids
• Phenols
• Volatile free acids
Temperature limits 40 to 250/260 °C (isothermal/TPGC)
USP phase G35
• High polarity column
• Provides better peak shape for underivatized acids
• Bonded, solvent rinsable
Stability • Free fatty acids • Replaces OV-351

Nitroterephthalic Acid
Modified Polyethylene Glycol

ZB-BAC1 & 2 • Blood alcohol analysis Temperature limits -20 to 260/280 °C (isothermal/TPGC) • Specifically designed for blood alcohol analysis
Proprietary

• Glycols • More accurate results for post mortem samples

Proprietary Bleed
• Abused inhalant anesthetics
More accurate results for blood alcohol Temperature Limits • Resolves t-butanol and n-propanol, for greater selection of internal standards
• Industrial solvents
analysis in post mortem samples Stability • Fast run time with baseline resolution of key components in just 2 minutes
• Enhanced resolution of ethanol and acetone peaks
~exclusive~

ZB-Drug-1 Proprietary Bleed

• Drugs of abuse
• General drug screen
• Designer drugs
Temperature limits -60 to 320/340 °C • Provides fast analysis with great peak shape
• Improves resolution of target analytes from matrix interferences
Optimized phase for the Temperature Limits • Specially deactivated to improve quantitation for drug compounds
separation of drugs of abuse Stability

2009
WINNER
~exclusive~

ZB-Bioethanol Proprietary Bleed

• Bioethanol
• Alcohol
• Fusel alcohols
• ASTM D 5501
• ASTM D 4806
• prEN 15376
Temperature limits -60 to 340/360 °C (isothermal/TPGC) • Provides accurate and reproducible results for Certificate of Analysis (COA)
• Resolve methanol and ethanol from all other denaturant peaks
Fast and accurate bioethanol analysis Temperature Limits • Great resolution of fusel alcohols
Stability

2009
WINNER
~exclusive~

www.phenomenex.com
PO48811109_L

Zebron, Inferno, Guardian, WAXPLUS , and MultiResidue are trademarks of Phenomenex, Inc. © 2010 Phenomenex, Inc. All rights reserved.