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©2001 Rohm and Haas Company - 10/01

Regarded as Nature’s catalysts,
enzymes are:

highly selective
stereospecific
perform complicated reactions:
neutral pH
ambient temperatures
atmospheric pressure
aqueous environment.

A key aspect of any process is the

capability to re-use the enzyme.
 Original product Enzyme reaction

New product Impurities Original product

Crystallisation Recycle
Advantages
Easy separation from reaction mixture
Re-use of enzyme for many reaction cycles
Lower cost of enzyme
Selective reactions
Increased stability of enzyme
Known engineering technology

Disadvantage
Lower activity than free enzyme
General observations

Macroreticular structure
Weakly ionic or non functionalized resins
Relatively hydrophilic surface : acrylic and phenolic
resins
Particle size important for kinetic reasons

Apart from the first point, there are exceptions to

each of the above.
Methods of fixation

Simple adsorption onto a buffered resin.

Adsorption onto a buffered resins followed by
treatment with glutaraldehyde.
Ionic bonding ( with H+ or OH- form resins).
Covalent bonding via -NH2 groups.
Via metals such as Fe, Ni, Al etc.
 Engineering Technology

Fixed
FixedBed
Bed Fluidised
FluidisedBed
Bed
The use of resins to immobilize enzymes have
found uses in many different industries such as:

Food
Sugar
Pharmaceutical
Chemical processing
Waste treatment
• Duolite XAD761 ß-amylase, ß-Galactosidase,
Lactase, Papain, Chymotrypsin,
Glucoseoxidase, Lipase.

• Amberlite IRC50 Lysozyme (recovery),

Cytochrome C, Acylase

• Amberlite XAD7 HP Thermolysin, Penicillin

acylase, Lipase, ß-amylase
• Amberlite IRA938*
Pepsin, Pepsinogen

• Duolite A568
Glucose Isomerase, Lipases

• Duolite A7
Trypsin, Aspartase, Aminocylase,
RNase, Lactase.

* Not Currently Manufactured

Rohm and Haas Company makes no warranties, either expressed or implied as to the accuracy of
appropriateness of the data in this document and expressly excludes any liability upon Rohm and Haas
arising out of its use. We recommend that the prospective users determine for themselves the suitability of
Rohm and Haas materials and suggestions for any use prior to their adoption.
Suggestions for uses of our products or the inclusion of descriptive material from patents and the citation of
specific patents in this publication should not be understood as recommending the use of our products in
violation of any patent or as permission or license to use any patents of the Rohm and Haas Company.
Material Safety Data Sheets outlining the hazards and handling methods for our products are available on
request.
Amberlite and Duolite are trademarks of Rohm and Haas Company, Philadephia.
The present document is copyrighted by Rohm and Haas and shall not be reproduced without a written
authorisation of Rohm and Haas Company.

©2001 Rohm and Haas Company