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VIBRIO
o
cholera is
endemic Cholera Toxin (A2-5B)(Vibrio cholerae)
• Chromosomally-encoded; Lysogenic phage
Taxonomy of conversion; Highly conserved genetic sequence
Vibrio cholerae • Structurally & functionally similar to ETEC LT
• >200 • B-subunit binds to GM1 ganglioside receptors in
serogroups small intestine
based on
somatic O-
• Reduction of disulfide bond in A-subunit activates A1
antigen fragment that ADP-ribosylates guanosine
triphosphate (GTP)-binding protein (Gs) by
• O1 and O139 transferring ADP-ribose from nicotinamide adenine
serogroups dinucleotide (NAD)
are responsible
for classic
• ADP-ribosylated GTP-binding protein activates adenyl
epidemic cyclase leading to an increased cyclic AMP (cAMP)
cholera level and hypersecretion of fluids and
electrolytes
• O1 serogroup
subdivided Mechanism of Action of Cholera Toxin
into
• Two biotypes:
El Tor and
classical (or
cholerae)
• Three
serotypes:
ogawa, inaba,
hikojima
• Some O1
strains do not
produce cholera
1
enterotoxin
2
(atypical or
nontoxigenic
O1 V. cholerae)
• Other strains
are identical to
O1 strains but
do not
agglutinate in
...Driving at night is a little bit like life. You can only see as far as the headlights shine but somehow, you make it ^_^ shar ^_^
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Micr
VIBRIO
o
O1 antiserum
(non-cholera
(NCV) or non-
agglutinating(N
AG) vibrios)
(non-O1
V.cholerae)
• Several phage
types
Epidemiology of
Vibrio cholera
• Cholera 3 4
recognized for
more than two
millennia with
sporadic
disease and
epidemics
• Endemic in
regions of
Southern and
Southeastern
Asia; origin of
pandemic
cholera
outbreaks
• Generally in 5
NOTE: In step #4, uptake of Na+ and Cl- from the lumen
communities
is also blocked. HCO3- = bicarbonate which provides
with poor
buffering capacity.
sanitation
• Seven
pandemics
(possible
beginning of
8th) since 1817
attributable to
increased world
travel
• Cholera spread
...Driving at night is a little bit like life. You can only see as far as the headlights shine but somehow, you make it ^_^ shar ^_^
through. 3
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VIBRIO
o
by
contaminated
water and
food
• Human
carriers and
environmenta
l reservoirs
Recent Cholera
Pandemics
• 7th pandemic:
o V.
cholerae
O1
biotype El
Tor
o Began in
Asia in
1961
o Spread to
other
continents
in 1970s
and 1980s
o Spread to
Peru in
1991 and
then to
most of
South &
Central
America
and to U.S.
& Canada
o By 1995 in
the
Americas,
>106
cases; 104
...Driving at night is a little bit like life. You can only see as far as the headlights shine but somehow, you make it ^_^ shar ^_^
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VIBRIO
o
dead
• 8th pandemic
(??)
o V.
cholerae
O139
Bengal is
first non-O1
strain
capable of
causing
epidemic
cholera
o Began in
India in
1992 and
spread to
Asia,
Europe and
U.S.
o Disease in
humans
previously
infected
with O1
strain, thus
no cross-
protective
immunity
...Driving at night is a little bit like life. You can only see as far as the headlights shine but somehow, you make it ^_^ shar ^_^
through. 5
Micr
VIBRIO
o
...Driving at night is a little bit like life. You can only see as far as the headlights shine but somehow, you make it ^_^ shar ^_^
through. 6
Micr
VIBRIO
o
...Driving at night is a little bit like life. You can only see as far as the headlights shine but somehow, you make it ^_^ shar ^_^
through. 7