Pierce ECL Western Blotting Substrate

A reliable ECL formulation without inflated prices.

Paying twice what you should for an enhanced chemiluminescent ECL substrate? For researchers interested
in a quality product at a fair price, there is a new option available. Pierce ECL Western Blotting Substrate is
an entry-level substrate that is value-priced.

If you are currently using a needlessly expensive entry-level ECL substrate, you can switch to Pierce ECL
Western Blotting Substrate without any optimization. Simply switch out the substrates and save a bundle.

Highlights:
• Half the cost of other ECL Substrates – low overhead and a commitment to customer
value enables Pierce to make this product available to you for half of the cost other
companies charge (these claims are based on the 2007 U.S. list prices)

• No optimization required – you can switch to our ECL substrate from other entry-level
ECL substrates, without the need for optimization or protocol changes

• Pierce, a brand trusted for more than 50 years, puts both its strong technical support
and reputation behind this product

Figure 1. Pierce ECL Substrate Western blot detection of Actin (Beta) from HeLa cell lysate.
Dilutions of HeLa cell lysate were prepared and separated by electrophoresis. The proteins were transferred to nitrocellulose membranes
(VWR Cat. No. PI88025) and the membranes were blocked with 5% skim milk. After blocking, the membranes were incubated with Mouse
Anti-Human Actin β (US Biological, Swampscott, MA) at 1 μg/ml. The membranes were washed and then incubated with 0.2 μg/ml of HRP-
conjugated Goat anti-Mouse IgG (VWR Cat. No. PI31430) and then washed again. Working solutions of the substrates were prepared
according to the manufacturers’ instructions and added to the membranes for 1 minute. The membranes were removed from the substrates
and placed in plastic sheet protectors. Each membrane was exposed to CL-XPosure Film (VWR Cat. No. P134090) for 90 seconds.

Ordering Information
Description Size, mL VWR Cat. No. List Price
Pierce ECL Western Blotting Substrate
50 PI32109 $50.00
Sufficient reagent for 4,000 cm2 of membrane
Pierce ECL Western Blotting Substrate
250 PI32209 $116.13
Sufficient reagent for 2,000 cm2 of membrane
Pierce ECL Western Blotting Substrate
500 PI32106 $161.42
Sufficient reagent for 400 cm2 of membrane
Figure 2. Pierce ECL Substrate Western blot detection of β-galactosidase (expressed) from Escherichia coli lysate.
Dilutions of E. coli cell lysate were prepared and separated by electrophoresis. The proteins were transferred to PVDF membranes
(VWR Cat. No. PI88585) and the membranes were blocked with 5% skim milk. After blocking, the membranes were incubated with
Mouse Anti-β galactosidase AB-1(Lab Vision, Fremont, CA) at 1 μg/ml. The membranes (VWR Cat. No. PI31430) and then washed
again. Working solutions of the substrates were prepared according to the manufacturers’ instructions and added to the membranes
for 1 minute. The membranes were removed from the substrates and placed in plastic sheet protectors. Each membrane was exposed
to CL-XPosure Film (VWR Cat. No. PI34090).

0907 5M Lit. No. 16029