Problem statement : What is the concentration of sucrose solution that can maintain the mass of plant tissue i.

e
potato?
Objective : To determine the concentration of sucrose solution that can maintain the mass of plant
tissue i.e potato ?
Variables:
( a ) Manipulated : Concentration of sucrose soluton
( b ) Responding : Change in mass of potato cylinder
( c ) Fixed : Volume of sucrose solution, same immersing time and surrounding
temperature, type of potato
Hypothesis : The higher the concentration of sucrose solution, the lesser the mass of potato cylinder
Apparatus : Cork borer, 50ml beaker, measuring cylinder, filter paper, weighing
electronic scales, ruler, knife, stop watch.
Material : Potato, 0.5%, 5.0%,10.0%,15.0%, 20% and 25.0% of sucrose solution

Technique : To weigh the changes in mass of each potato cylinder by using weighing electronic scale
before and after immersing in different sucrose solution.

Method/procedure
1 A piece of medium –size potato is chosen and strips of potato are made with the cork-borer. Twelve strips of
cylinder of 5cm were obtained.
2 Every potato cylinder is dried with filter paper, then weighed to obtain the initial mass.
3 Six beakers are prepared and labelled A, B, C, D, E and F . Every beaker is filled with 20cm3 of 0.5%,
5.0%,10.0%, 15.0%, 20.0% and 25.0% of sucrose solution respectively.
4 Two potato cylinders are placed into each beaker
5 After being immersed for one hour, every potato cylinder is removed, dried with filter paper, then weighed
and measured to obtain the final reading.
6 The results are recorded in a table
7 Plot the graph of concentration of sucrose solution against the mass of the cylinder.

Presentation of data
Concentration of Beaker mass of potato (g) Difference mass of cylinder
sucrose solution/% (g)
Initial Final
0.5 A
5.0 B
10.0 C
15.0 D
20.0 E
25.0 F

Conclusion
Based on the plotted graph, the line which intersects the horizontal-axis (mass-axis) indicates that there is no
change in the mass of the potato cylinders
This proves that the cell sap of potato cylinder is isotonic to the concentration of the sucrose solution at that
particular point.
Problem statement : What is the concentration of sucrose which is isotonic to the cell sap of
mango?
Objective : To study the concentration of sucrose which is isotonic to the cell sap of mango
Hypothesis : The concentration of sucrose solution which is isotonic to the cell sap of mango
is a point where there is no nett change in mass of the mango strip.
Variables
( a ) manipulated : Concentration of sucrose solution
( b ) responding : Change in mass of mango strips
( c ) fixed : Volume of sucrose solution, same immersing time and surrounding
temperature, type of mango

Apparatus : Cork puncher, petri dish, measuring cylinder, filter paper, weighing scales, knife,
ruler, stopwatch
Material : Mangoes, sucrose solution 2%,4%,6%,8% and 10%, water.

Technique : To weigh the changes in mass of each mango strips by using weighing electronic
scale before and after immersing in different sucrose solution.
Method/procedure
1 Five petri dishes are labelled A,B,C,D and E.
2 Fill each petri dish with sucrose solution as follows:

Petri dish Sucrose solution

A 20 ml of 2% sucrose solution
B 20 ml of 4% sucrose solution
C 20 ml of 6% sucrose solution
D 20 ml of 8% sucrose solution
E 20 ml of 10% sucrose solution

3 Use cork puncher to obtain five cylindrical strips of mangoes

4 Cut each strip such that the length is 40 mm respectively
5 Weigh each strip and record its initial mass
6 Put each strip into the respective petri dishes and leave it for 30 minutes
7 Take out the strips and dry the strips with filter paper
8 Weigh the strips that has been dried up
9 Record the results in a table
10 Plot the graph of concentration of sucrose solution against the mass of the strips

Presentation of data
Petri dish Initial mass of strip (g) Final mass of strip (g) Difference in mass of strip (g)
A
B
C
D
E

Conclusion
The concentration of sucrose solution which is isotonic to the cell sap of mango is a point where there is no nett
change in mass of the mango strip.