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College of Science
Department of Chemistry
Course Book
Instrumental Analysis for fourth Stage
M.Sc. in Chemistry
Ph.D. in Chemistry
Academic Year: 2010-2011
Assist. Prof. Dr. Kamal Mustafa Mahmoud
E-mail: dr.kamal@uni-sci.org
or kamal_k58@yahoo.com
Mob: +964 (0) 750 454 6198
(Academic Year 2010-2011)
Grading
Number of Units = 8
Theory:
• Three term exams 20 points
• Quizzes 05 points
• Final exam 50 points
Practical:
• Term exam 10 points
• Oral Presentation & seminars 05 points
• Final exam 10 points
• Total (Theory + Practical) 100 points
Course Program
∑ (Xi - X-)2
Absolute standard deviation: s = √
N-1
Variance: S2
S
LOL
Dynamic range: 10² to > 10⁶
LOQ
Selectivity
No analytical method is completely free from interference
by concomitants. Best method is more sensitive to
analyte than interfering species (interferent).
Matrix with species A&B: Signal=mAcA + mBcB+ Signalblank
mB
Selectivity coefficient: kB,A=------
mA
k’s vary between 0 (no selectivity) and large number (very
selective).
Calibration methods
Basis of quantitative analysis is magnitude of
measured property is proportional to
concentration of analyte
Signal α[x] or Signal =[x]+ Signalblank
Regression equation: y = a + m X
a = intercept = y- - mX-
m = slope = ∑ (Xi – X-)(yi –y-) ⁄ ∑ (Xi – X-)2
Example :
Calibration expression is