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LYNDA L. RENNIE-TANKERSLEY, M.S.


Home Address: 209 Hunters Ridge Road
Timonium, MD 21093
Mobile Phone: #410-698-3974
E-mail: lr4cfc18@westpost.net
CAREER OBJECTIVE: Medical research position involving molecular and cellular bio
logy
SUMMARY OF QUALIFICATIONS: I previously worked for The Johns Hopkins University
as a research scientist and laboratory manager. I obtained my Masters of Scien
ce Degree in Biotechnology from The Johns Hopkins University, while working ther
e full-time, and have acquired over twenty peer reviewed journal article publica
tions. I have extensive experience in the industry, overseeing MD and PHD fello
ws, training them in the latest molecular biology techniques. A few highlights
of the knowledge and techniques I have proven/developed through my experience ar
e; 1) the production of collagen in the liver produces cirrhosis, 2) the latest
Taqman chemistry for real-time PCR and 3) ELISA methods for novel genes, collabo
rating with private industry for production.
COMPETENCE & EXPERTISE:
*Experienced with advanced Molecular Biology techniques
*Technical leadership expertise in both academia and private industry for R&
D
*Experiences in the pharmaceutical, agricultural, manufacturing, chemical, petro
leum, food, coatings, polymer, and refinery industries as well as academia
*Skilled at assay design, development, implementation, optimization, and analysi
s
*Cultivation of the team as well as independent research
*Great ability to be flexible proven by experience working in a small company
*Varied experiences due to extensive exposure to world reknown knowledge and vas
t facilities at Johns Hopkins University
*Confident communication style in both writing and speaking
*Ability to motivate and lead individuals
*Experienced with feasibility studies to validate analytical chemistry methods
*Experienced with protein, peptones, media components, and fractionation
*Experience creating process control solutions in private industry
*Management experience
*Over two years sales and marketing experience in medical & scientific communiti
es
*Great flexibility to learn new equipment and apply old knowledge
*Strong background in experimental biology
*Success in bringing new knowledge to internal as well as external arenas.
EDUCATION:
Johns Hopkins University,Baltimore, Maryland
M.S. Masters of Science in Biotechnology
Western Maryland College,Westminster, Maryland
B.A. Bachelors of Liberal Arts in Biology
All American Swimmer
Dulaney Senior High School, Timonium, Maryland
H.S. Advanced Biology, Biochemistry, Calculus
WORK EXPERIENCE:
Sales distributor and business owner:
MarketAmerica.com; York, PA
07/2009 - present
Health and Life Insurance agent:
HealthMarkets; York, PA
01/2009 - 02/2010
Industrial and Pharmaceutical Spectrometry Engineer:

Brimrose Corp Of America; Sparks, Maryland


09/2006 - 07/2008
SUMMARY OF DUTIES:
Trained customers, participated in tradeshows, wrote feasibility studies, and pe
rformed chemometric modeling, for thin film coating analysis, the pharmaceutical
industry, the petroleum industry, the agricultural industry, the polymer indust
ry, the chemical industry, refineries, and the food industry.
*Provided technical customer support for applications in NIR Spectrometry
*Prepared audits for pharmaceutical companies
*Performed software configuration and quality control management
*Met targeted sales growth goals
Laboratory Manager, Cardiology:
Johns Hopkins University; Baltimore, Maryland
11/2005 - 8/2006

SUMMARY OF DUTIES:
Developed PCR, Real-time PCR, and Taqman chemistry for large laboratory. Perfor
med Electrophoresis using SDS-PAGE, native, agarose, two-dimensional, and capill
ary methods. Developed Molecular biology techniques such as plasmid preparation
s, restriction digest analysis, DNA sequencing, in-vitro transcription/translati
on, transformation, immunoblotting, and basic viral techniques. Carried out gen
eral microbiology and basic cell culture, media preparations, culturing techniqu
es, and culture maintenance. Chromatography was performed using; HPLC: methods,
development, and troubleshooting; TLC; solid-phase extraction (SPE); and paper
affinity. Protein isolation/purifications were performed, as well as immunologi
cal techniques, Radiochemical usage and handling; and Southern, Western, and Nor
thern blots; Dot Blot analysis; densitometry; nucleic acid hybridization; fluore
scence microscopy; radioimmunoassay; and ELISA. Computer skills include experie
nce with many Windows-based applications, internet molecular biology and protein
databases as well as literature searches.
*Laboratory management
*Study the effects of Viagra on the heart
*Train Ph.D. and M.D fellows
*Neonatal myocyte isolation
*Animal breeding and genotyping
Senior Scientist II / Laboratory Manager, Gastroenterology:
Johns Hopkins University; Baltimore, Maryland
11/1991 - 11/2005

SUMMARY OF DUTIES:
Primer / probe design, Tissue Culture, Aseptic Techniques, Real-time PCR / PCR,
Southern/Northern/Western, Electromobility Shift Assays (EMSA), RNA / DNA prepar
ation, Densitometry Imaging, Biochemical Assays, Cloning, Nuclear Protein prepar
ation, Chromatography, Genetic Engineering, Radiolabeling, ELISA development, Si
te-directed mutagenesis, Antibody isolation and purification, Enzymatic Assays,
Gas Chromatograph, Gel Electrophoresis, Gene Targeting, Image Analysis, Lymphocy
te isolation, Mass Spectrophotometer
Microscopy, Protein-DNA Interaction Analysis, Protein Isolation and Analysis, Nu
cleic Acid Labeling, RNase Protection Assay, Radiation Safety Monitoring, Taqman
chemistry, Restriction mapping, Enzyme Analysis, SDS-page, Sequencing analysis,
Spectrophotometry, Sub-Cloning, Transfection Assays, Transformation, Vector Des
ign, and Construction, Flourometry, Confocal Microscopy
*Train Ph.D. and M.D fellows
*Develop and perform protocols, present data
*Interpret clinical research by contributing in peer-reviewed journal articles
*Consult with clinicians and scientists in order to initiate new ideas
*Study the mechanisms associated with alcoholic liver disease
Senior Research Scientist, Epidemiology:
Johns Hopkins University; Baltimore, Maryland
SUMMARY OF DUTIES:
*Conducted epidemiological multi-center clinical trials associated with HIV
*Developed and optimized ELISA assays for the detection of HCV
*Presented seminars both within Johns Hopkins and at scientific meetings
*Cultured live HIV virus in P2 and P3 facilities
Research Scientist, Physiology
Johns Hopkins University;Baltimore, Maryland
SUMMARY OF DUTIES:
*Interpreted the effects of different pharmaceuticals on asthma
*Administered drugs to asthmatic dogs
*Led in-vivo clinical trials on basenji-greyhound dogs
*Ran Autoanalyzer for histamine
Other Skills:
Adobe Photoshop, Microsoft Office including Excel, Word, PowerPoint, Primer and
Probe design software, scientific databases
Work Status: I am authorized to work anywhere in the US and I am a US citizen.
References: Available upon request

Original Publications:
Rikimaru T, Nakamura M, Yana T, Beck G, Habicht GS, Rennie LL, Widra M, Hirshman
CA, Boulay MG, Spannhake EW, et al. Mediators, initiating the inflammatory resp
onse, released in organ culture by full-thickness human skin explants exposed to
irritant, sulfur mustard. J Invest Dermatol 96(6): 888-97, 1991.
Donahue JG, Nelson KE, Munoz A, Vlahov D, Rennie LL, Taylor EL, Saah AJ, Cohn S,
Odaka NJ, Farzadegan H. Antibody to hepatitis C virus among cardiac surgery pat
ients, homosexual men, and intravenous drug users in
Baltimore, Maryland. Am J Epidemiology 134(10):1206-11, 1991.
Mezey E, Sharma S, Rennie L, Potter JJ. Sex differences in rat gastric alcohol d
ehydrogenase activity. Gastroenterology 103:1804-1810, 1992.
Pares A., Potter JJ, Rennie L, Mezey E. Acetaldehyde activates the promoter of t
he mouse a2(I) collagen gene. Hepatology 19:498-503, 1994.
Anania FA, Potter JJ, Rennie-Tankersley L, Mezey E. Effects of acetaldehyde on n
uclear factor I and its binding to the a2(I) collagen promoter. Hepatology 21:16
40-1648, 1995.
Anania FA, Potter JJ, Rennie-Tankersley L, Mezey E. Activation by acetaldehyde o
f the promoter of the mouse a2 (I) collagen gene in rat myofibroblast-like cells
. Archives Biochem Biophys 331: 187-193, 1996.
Miao K, Potter JJ, Rennie-Tankersley L, Mezey E. Reduced SP1 binding by acetalde
hyde in the regulation of the a2(I) collagen promoter. Archives Biochem Biophys
341:140-152, 1997.
Mezey E, Rennie-Tankersley L, Potter JJ. Effect of dihydrotesterone on turnover
of alcohol dehydrogenase in rat hepatocyte culture. Hepatology27:185-190, 1998.
Miao K, Potter JJ, Anania FA, Rennie-Tankersley L, Mezey E. Identification of t
wo repressor elements in the mouse a2 (I) collagen promoter. Arch Biochem Biophy
s361:7-16, 1999.
Potter JJ, Anania FA, Rennie-Tankersley L, Mezey E. A transient increase in c-my
c precedes the transdifferentiation of hepatic stellate cells to myofibroblast-l
ike cells. Liver 19:135-144, 1999.
Wang L, Attard FA, Rennie-Tankersley L, Potter JJ, Mezey E. Effect of retinoic a
cid on the enhancing effect of acetaldehyde on mouse type I collagen expression.
Arch Biochem Biophys 367: 191-198, 2000.
Attard FA, Wang L, Rennie-Tankersley L, Potter JJ, Mezey E. CCAAT/enhancer bind
ing protein mediates the activation of the murine a1 (I) collagen promoter by ac
etaldehyde. Arch Biochem Biophys378: 57-64, 2000.
Attard FA, Wang L, Potter JJ, Rennie-Tankersley L, Mezey E. Identification of tw
o new sites of binding and activation of the murine a1 (I) collagen promoter by
CCAAT/enhancer binding protein. DNA Cell Biol. 20:455-463, 2001.
Mezey E, Rennie-Tankersley L, Potter JJ. Liver alcohol dehydrogenase is degraded
by the ubiquitin-protease pathway. Biochem Biophys Res Commun 285:644-648, 2001
.
Wang L, Rennie-Tankersley L, Tang M, Potter JJ, Mezey E. Regulation of the murin
e a2 (I) collagen promoter by retinoic acid and retinoid X receptors. Arch Bioch
em Biophys 401:262-270, 2002.
Potter JJ, Rennie-Tankersley L, Mezey E. Endotoxin enhances liver alcohol dehydr
ogenase by action through upstream stimulatory factor but not by nuclear factor-
B. J Biol Chem 278 No 6: 4353-4357, 2003.
Potter JJ, Rennie-Tankersley L, Mezey E. Influence of leptin in the development
of hepatic fibrosis produced in mice bySchistosoma mansoni infection and by chro
nic carbon tetrachloride administration. Journal of Hepatology 38: 281-288, 2003
.
Mezey E, Potter JJ, Rennie-Tankersley L, Caballeria J, Pares A. A randomized pla
cebo controlled trial of Vitamin E for alcoholic hepatitis. J Hepatol. 40:40-46,
2004.
Novitskiy, G., Potter JJ, Rennie-Tankersley L, Mezey E. Identification of a nove
l NFkB binding site with regulation of the murine a2 (I) collagen
promoter. J Biol Chem. 279:15639-15644, 2004.
Potter JJ, Rennie-Tankersley L, Mezey E. Leptin deficiency prevents the activati
on of the murine a2 (I) collagen promoter by acetaldehyde. Arch. Biochem. Biophy
s 426:73-77,2004.
Wang L, Rennie-Tankersley L, Tang M, Potter JJ, Mezey E. Regulation of a2 (I) co
llagen expression in stellate cells by retinoic acid and retinoid X receptors th
rough interactions with their cofactors. Arch. Biochem.
Biophys. 428:92-98, 2004.
Novitskiy, G., Ravi, R., Potter JJ, Rennie-Tankersley L, Wang L., Mezey E. Effec
ts of acetaldehyde and TNFa on inhibitory kappa B-protein and nuclear factor kap
pa B activation in hepatic stellate cells. Alcohol and Alcoholism, 40:96-101, 20
05.

Abstracts:
Pares A, Potter JJ, Rennie L, Mezey E. Activation of the mouse a2 (I)collagen ge
ne promoter by acetaldehyde. Hepatology 18:152A, 1993. Anania FA, Potter JJ, Re
nnie-Tankersley L, Mezey E. Acetaldehyde enhances nuclear factor I (NF-I) bindin
g to the mouse a2 (I) collagen promoter. Gastroenterology 106(4), 1994.
Anania FA, Potter JJ, Rennie-Tankersley L, Mezey E. Acetaldehyde acts independen
tly of TGFb1 in enhancing type I collagen message in rat myofibroblast-like (MFL
) cells. Hepatology 20:858, 1994.
K. Miao, Potter JJ, Anania FA, Rennie-Tankersley L, Mezey E. Recognition of two
SP1 binding sites upstream to the nuclear protein I (NF-I) binding site in the
mouse a2 (I) collagen promoter. Hepatology 22:699, 1995.
Anania FA, Potter JJ, Rennie-Tankersley L, Mezey E. Acetaldehyde (AC)potentiates
protein kinase C (PKC) activity in rat myofibroblast-like (MFL) cells. Gastroen
terology 110:A1142, 1996.
K. Miao, Potter JJ, Rennie-Tankersley L, Anania FA, Mezey E. Effect of acetaldeh
yde on SP1 regulation of the a2 (I) collagen promoter. Hepatology 24:463A, 1996.
Anania FA, Potter JJ, Rennie-Tankersley L, Mezey E. Acetaldehyde activates prote
in kinase C activity in rat hepatic stellate cells through diacylglycerol produc
tion. Hepatology 24:184A, 1996.
Potter JJ, Rennie-Tankersley L, Anania FA, Mezey E. Transdifferentiation of stel
late cells to myofibroblast-like (MFL) cells is associated with a fall in the ex
pression of c-fos and c- myc. Hepatology 26: 190A, 1997.
K. Miao, Potter JJ, Rennie-Tankersley L, Anania FA, Mezey E. A novel negative re
gulatory element in the promoter of the mouse a2(I) collagen gene. Hepatology 26
: 378A, 1997.
L. Wang, Potter JJ, Rennie-Tankersley L, Mezey E. Influence of retinoic acid (RA
) on the enhancing effect of acetaldehyde (AC) on type I collagen expression. He
patology 28: 537A, 1998.
F. Attard, Potter JJ, Rennie-Tankersley L, Mezey E. Acetaldehyde increases C/EBP
protein in hepatic stellate cells and C/EBPbinding to the a1 (I) collagen promot
er. Hepatology 28: 597A, 1998.
F. Attard, Potter JJ, Rennie-Tankersley L, Mezey E. Role of C/EBP in activation
of the a1 (I) collagen promoter a1 (I) CP by acetaldehyde (AC) in cultured hepat
ic stellate cells (HSC). FASEB J 13:A196, 1999.
F. Attard, Wang L, Rennie-Tankersley L, Potter JJ, Mezey E. Activation of the mu
rine a1 (I) collagen promoter by acetaldehyde (AC) in cultured hepatic stellate
cells involves C/EBP. Hepatology 30:491A, 1999.
Mezey E, Potter JJ, Rennie-Tankersley L, Caballeria J, Pares A. A randomized pla
cebo controlled trial of Vitamin E for alcoholic hepatitis. Hepatol 38:225, 2003
.
Novitskiy, G., Ravi, R., Potter JJ, Rennie-Tankersley L, Wang L., Mezey E. Effec
ts of acetaldehyde and TNFa on inhibitory kappa protein and nuclear factor kappa
B activation in hepatic stellate cells. Hepatology (submitted).