CURRICULUM VITAE

Hsi-Ping Li, Ph.D.

9 Gibson Ct
Gaithersburg, MD 20878
Mobile: 240-750-0700
Evening Phone: 301-977-1159
Day Phone: 301-435-8787
Email: hl549a86@westpost.net

SUMMARY OF QUALIFICATIONS
Over 20 years experience in lab research at different research institutions, inc
luding 15 years of research in the lab of Dr. Marshall Nirenberg, a Nobel laurea
te and discoverer of genetic code, at NIH.
Cell biology: Culture of chicken neural retina cells, human glioma cells, HEK 29
3 cells, mouse hybridoma cells, immunostaining, fluorescence microscopy, electro
n microscopy, vector transfection, monoclonal antibody production, ELISA, soft a
garose culture and immunoadsorption assay.
Molecular biology: DNA purification, DNA cloning, RNA purification, DNA sequenci
ng, genetic and cDNA library screening, Southern and Northern blotting, PCR/RT-P
CR/real-time PCR, inverse PCR, site-directed mutagenesis, design and generation
of recombinant vectors.
Protein biochemistry: Chromatography, SDS-PAGE, Western blotting, protein assay,
mobility shift assay, in vitro protein phosphorylation assay, recombinant prote
in production.
Drosophila techniques: Drosophila culture, P-element-mediated mutagenesis, embry
o immunostaining, probe labeling, in situ hybridization, embryo injection, micro
array, RNAi, zinc finger nuclease.
Computer skills: Microsoft Word, Excel, PowerPoint, Adobe Photoshop, PubMed, BLA
ST, EndNote, GeneSpring, Biology WorkBench and other computer and web-based soft
ware for experimental data collection, processing, analysis and presentation.
WORK EXPERIENCE
Lab of Biochemical Genetics, NIH/NHLBI, Bethesda, MD
12/2000 - Present
Staff Scientist
Studying development of Drophila nervous system and Drosophila embryo gene regul
ation using RNAi, microarray, zinc finger nuclease and other techniques.
Department of Medicine, Division of Nephrology, Mount Sinai School of Medicine,
New York, NY
10/1996 - 11/2000
Research Assistant Professor
Performing studies in identifying the putative phosphorylation sites of human pr
otein polycystin-1 C-terminal domain (PKD1-CTD), cloning and functional study of
human PRKX, a member of cAMP-dependent serine threonine kinases family.
Lab of Biochemical Genetics, NIH/NHLBI, Bethesda, MD
9/1990 - 9/1996
Visiting Associate
Establishing over 500 transgenic Drosophila mutant lines through insertion of P-
element into Drosophila genome. Cloning of several novel genes that are expresse
d in Drosophila nervous system by screening of Drosophila genomic DNA and cDNA l
ibraries.
Department of Cell Biology, Beijing Neurosurgical Institute, Beijing, China
2/1985 - 8/1990
Assistant Professor
Production and characterization of monoclonal antibodies against human malignant
glioma and cytoskeletal proteins. Study of the cytoskeleton of BT325, a human g
lioma cell line using cell and immunological techniques.
Lab of Biochemical Genetics, NIH/NHLBI, Bethesda, MD
1/1984 - 1/1985
Visiting Fellow
Generation of monoclonal antibodies against chicken neural retina using novel so
ft agarose culture and immunoadsorption assay techniques.
EDUCATION
Department of Biology, Temple University
Philadelphia, PA US
Ph.D. - 12/1983
Major: Biology
Shanghai Medical University
Shanghai, China
M.D. - 8/1975
Major: Medicine
PROFESSIONAL PUBLICATIONS
Li HP and Sheffield JB (1982) Delayed appearance of fibronectin in stationary cu
ltures of embryonic retina cells. J. Cell Biol. 95:30a.
Li HP and Sheffield JB (1984) Isolation and characterization of flat cells, a su
bpopulation of the embryonic chick retina. Tissue Cell 16:843-857.
Li HP and Sheffield JB (1986) Retinal flat cells are a substrate for retinal neu
ron growth and fiber formation. Invest. Ophthalmol. Vis. Sci. 27:296-306.
Li HP and Sheffield JB (1986) Retinal flat cells participate in the formation of
fibers by retinal neuroblasts in vitro. Time lapse video studies. Invest. Ophth
almol. Vis. Sci. 27:307-315.
Sheffield JB and Li HP (1987) Interactions among cells of the developing neural
retina in vitro. Amer. Zool. 27:145-159.
Sheffield JB, Graff D and Li HP (1987) A solid phase method for the quantitation
of protein in the presence of SDS and other interfering substances. Anal. Bioch
em. 166:49-54.
Shao, WZ, Li, HP, Zheng, YS, Li, JH, Zhang, Y, Luo, L, Wang, TY and Sheng, SL (1
987) Production and characterization of monoclonal antibodies against malignant
glioma. Chinese J. Neurosurg. 3:151-156.
Ning AL, Zhang XY, Wang XQ, Zhang YH, Wu M, Wang TY and Li HP (1988) Expression
of intermediate filaments in cultured cells. Acta Acad. Med. Sinicae 10:321-324.
Qi Y, Shao WZ, Li HP, Luo L, Li JH, Wei Q and Zheng YS (1990) Preparation and ap
plication of glial fibrilary acidic protein and its antibody. Chinese J. Neurosu
rg. 6:33-36.
Li HP, Liu ZM, Chen T and Nirenberg M (1990) Application of soft agarose culture
and immunoadsorption assay in the production of monoclonal antibodies against e
mbryonic chicken retina. Chinese J. Immunol. 7:118.
Li HP, Liu ZM and Shao WZ (1990) Immunofluorescence study of the cytoskeleton of
BT325, a human glioma cell line. Acta Anat. Sinica. 21:269.
Liu ZM and Li HP (1991) Study of the cytoskeleton of BT325 human glioma cells by
Carbon-Platinum coating and immunoelectron microscopy. Acta Anat. Sinica. 22:72
.
Chen L, Shao WZ, Li HP, Liu ZM, Zheng YS, Li, JH and Zhao JF (1991) Establishmen
t and characterization of hybridoma cell lines against neurofilament proteins. C
hinese J. Neurosurg. 7:208.
Li HP, Liu ZM, and Nirenberg M (1997) Kinesin-73 in the nervous system of Drosop
hila embryos. Proc. Natl. Acad. Sci. USA.94:1086-1091.
Li HP, Bloswick B, Burrow CR and Wilson PD (1998) Transient transfection of PKD-
1 C-terminal reporter constructs in renal epithelial cells. JASN 8:440a.
Li HP, Geng L, Burrow CR and Wilson PD (1999) Identification of phosphorylation
sites in the PKD1-encoded protein C-terminal domain. Biochem. Biophys. Res. Comm
. 259:356-363.
Geng L, Burrow CR, Li HP and Wilson PD (2000) Modification of the composition of
polycystin-1 multiprotein complexes by calcium and tyrosine phosphorylation. Bi
ochim Biophys Acta. 1535:21-35.
Li X, Li, HP, Amsler K, Hyink D, Wilson PD and Burrow, CR (2002) PRKX, a phyloge
netically and functionally distinct cAMP-dependent protein kinase, activates ren
al epithelial cell migration and morphogenesis. Proc. Natl. Acad. Sci. USA. 99:
9260-9265.
Kaletta T, Van der Craen M, Van Geel A, Dewulf N, Bogaert T, Branden M, King KV,
Buechner M, Barstead R, Hyink D, Li HP, Geng L, Burrow C, Wilson P. (2003) Towa
rds understanding the polycystins. Nephron Exp Nephrol. 93: e9-17.
Ivanov AI, Rovescalli AC, Pozzi P, Yoo S, Mozer B, Li HP, Yu SH, Higashida H, Gu
o V, Spencer M and Nirenberg M (2004) Genes required for Drosophila nervous syst
em development identified by RNA interference. Proc Natl Acad Sci USA 101: 16216
-16221.
Li HP, Rovescalli A, Yu s, Mozer B, Nadjmabadi R, Billings E and Nirenberg M (20
07) Different isoforms of lola proteins regulate different sets of genes. Abstra
cts of papers presented at the 2007 Meeting on Neurobiology of Drosophila, p149.
Li HP, Rovescalli A, Yu s, Mozer B, Nadjmabadi R, Billings E and Nirenberg M (20
10) Different species of lola mRNA encode proteins that regulate different sets
of genes. Manuscript is ready for publication.
Honors and awards
1979-1983 Recipient of the Chinese Academy of Sciences Ph.D. Program Scholarship
for outstanding graduate students pursuing Ph.D. Degree in US.
1982 Recipient of the travel fund award to 1982 ARVO meeting from the Associatio
n for Research on Vision and Ophthalmology (ARVO) for excellent abstract.
1987-1990 Recipient of the Achievements in Scientific Research Awards for subseq
uent four years from the Department of Health, Beijing Municipal Government.
1999 Recipient of Scientist Development Grant (1999-2002) from American Heart As
sociation.