THOMAS A.

SEIFLEIN
5098 Carriage Lane * Lockport, NY 14094 * tsf89a50@westpost.net * (71
6) 697-9498
http://www.jobfox.com/people/ThomasA-Seiflein
ACADEMIC TEACHING AND RESEARCH PROFESSIONAL
SUMMARY OF QUALIFICATIONS
* Dynamic educational professional with more than 10 years teaching and research
experience.
* Skilled at writing assessment questions, reports and demographic data collecti
on and analysis, evaluating student progress and curriculum development.
* Strong administration experience at workshop organization, event scheduling, d
esigning promotional materials, participant communications and applicant selecti
on and follow up.
* Superior laboratory management experience at maintaining lab stocks and orderi
ng lab supplies, hiring and training new personnel, scheduling lab and journal c
lubs meetings and resolving employee issues.
* Supervised lab personnel to ensure work meets standards and completed on time.
* Monitored use of equipment to ensure completion of experiments.
* Performed detailed cost comparison analysis and researched new products and se
rvices.
* Maintain laboratory equipment through coordination of technical services, fis
cal office and salesmen to order and repair laboratory equipment.
* Microbiology skill with aseptic and sterile technique, medias and solution pre
paration along with purification and quantification of bacteriophages.
* DNA Analysis software experience with web based DNA analysis tools including B
LAST, restriction maps, promoter prediction, primer design and DNA sequence edi
ting and contig alignments.
* Molecular Biology experience including DNA sequencing, real-time PCR, inverse
and bubble PCR, molecular cloning, recombinant plasmid construction, western and
southern blotting.
* Biochemistry knowledge in protein purification and enzyme assays.
* Cell biology experience including culturing mammalian cells, utilizing imunofl
oresence to stain cultured cells and transfection of mammalian cells.
* Genetics experience in insertion mutagenesis using Tn10 transposition and reco
mbinant bacteriophage delivery vectors, strain construction and linkage via tran
sduction with bacteriophages P1, P22, P52 and ES18, assay of reporter gene fusio
ns and design of both genetic screens and selections.
* Equipment experience including Roche Real-Time Light-Cycler480, Abbott Real-Ti
me HIV-1 Assay and Abbott m2000 System, Forma Scientific Anaerobic System, Appli
ed Biosystems automated DNA sequencer and computer integrated 96 well spectropho
tometer and luminescence spectrometer.
* Computer skills include MS Office Suite, Canvas, Adobe CS4, FileMaker Pro Adva
nced, Protein Analysis Software RasMol and Biology Workbench.
PROFESSIONAL EXPERIENCE
UNIVERSITY OF PITTSBURGH, Pittsburgh, PA
Research Specialist III August 2010 to Present
* Utilized a sensitive real-time PCR assay for determining HIV viral load in cli
nical HIV blood samples.
Outreach Coordinator for Department of Biological Sciences Nove
mber 2007 - April 2010
* Provide local high school teachers with cutting edge biology information and e
xperiments as part of the University of Pittsburgh's Outreach Program.
* Perform tasks include planning, organizing and training at summer workshops fo
r high school biology teachers to earn Pennsylvania Act 48 continuing education
credits.
* Coordinate Pitt Kits Program that supplies high school teachers with all mater
ials required to teach summer workshop experiments and information in their clas
srooms.
* Assist teachers in classrooms as a traveling laboratory instructor.
* Organize and teach on the Mobile Science Laboratory project, a 70 ft. mobile l
aboratory, which travels throughout western PA bringing current biology research
to local communities.
Instructor for Foundations of Biology Laboray I & II
August 2002 to December 2008
* Communicated passion for science as freshman lab instructor and encouraged stu
dents to pursue undergraduate research.
Research Specialist IV
January 2007 to October 2007
* Studied role of the protein NuMA in cancer using cell biology and biochemical
techniques.
* Managed laboratory and data preparation for grants and publications.
Research Specialist IV
April 1999 to November 2006
* Utilized genetic, molecular and biochemical techniques to elucidate pathways w
hich Klebsiella uses methionine as a sole sulfur source.
* Tasks included the day to day training, implementation and troubleshooting lab
oratory techniques with personnel.
* Mentored eight graduate and 21 undergraduate students in basic microbiology an
d molecular biology techniques including insertion mutagenesis, genetic screens,
strain construction via P1 mediated transduction, cloning and DNA sequencing.
* Provided direction for multiple research projects with team members of varying
degrees of knowledge and abilities.
* Networked with other laboratories and department office staff in order to expa
nd the resources available to laboratory.
EDUCATION AND TRAINING
STUDIES IN MOLECULAR, CELLULAR & DEVELOPMENTAL BIOLOGY PROGRAM UNIVERSITY OF PIT
TSBURGH, Pittsburgh, PA
BACHELOR OF ARTS IN BIOLOGY
BUFFALO STATE COLLEGE Buffalo, NY
* Graduated summa cum laude.
ADDITIONAL CLEARANCES, LICENSES, TRAINING AND CERTIFICATIONS
* Pennsylvania Clearances # 34 & # 151, issued 2/2009.
* Pennsylvania Child Abuse History Clearance, issued 3/2009.
* Pennsylvania Resident Producer Individual Insurance License: Accident and Heal
th, Life and Fixed Annuities, issued 7/2010.
* Chemical Safety Training.
* Blood-borne Pathogen Training.
* Dangerous Goods Shipping Training.
* Isotope Research Safety Training.
* Formaldehyde Safety Training.
PUBLICATIONS
* Seiflein, T. A. and J. G. Lawrence. 2006. Two transulflurylation pathways in K
lebsiella pneumoniae. J Bacteriol. 188(16):5762-74.
* Seiflein, T. A. and J. G. Lawrence. 2001. Methionine-to-cysteine recycling in
Klebsiella aerogenes. J Bacteriol. 183(1):336-346.