Curriculum Vitae for Sudeshna Ghosh

Postdoctoral Fellow, Department of Civil and Environmental Engineering University of Michigan, Ann Arbor, Michigan 48109 Phone: 651-785-5458, Email: sudghosh@umich.edu Ph.D., Civil Engineering, University of Minnesota, Twin Cities Thesis Title: Characterization of Antibiotic Resistance in Soils Exposed to Manure from Farms Using Subtherapeutic Antibiotics for Growth Promotion Adviser: Timothy M. LaPara M.S., Civil Engineering, University of Minnesota, Twin Cities Thesis Title: Removal of Carbonaceous and Nitrogenous Pollutants from a Synthetic Wastewater Using a Membrane-Coupled Bioreactor Adviser: Timothy M. LaPara B.S., Civil Engineering, Jadavpur University, Kolkata, India Nov 2007

Nov 2003

Aug 2000

PROFESSIONAL EXPERIENCE Postdoctoral Fellow, University of Michigan, Ann Arbor Research interests: Pathogens and public health, ecology of algae Adviser: Nancy G. Love Postdoctoral Fellow, California Institute of Technology, Jet Propulsion Laboratory, Pasadena Research interests: Ecology of clean-room bacteria Supervisor: James A. Spry Structural Design Engineer, Stup Consultants Ltd., India Oct 2008- present

Jan 2008- Sep 2008

Nov 2000- May 2001

PROPOSALS AND AWARDS Point-of-Use devices as incubators of halogenated phenol-mediated antibiotic resistant bacteria: National Science Foundation with Nancy G. Love and Terese M. Olson Investigating the relationship between structural diversity and functional resilience to stress in ammonia-oxidizers: National Science Foundation with Nancy G. Love, Lutgarde Ruskin and Ameet J. Pinto. NASA Tech Brief Award for NTR no 46337: Bacillus horneckii sp. nov., isolated from a clean room where Phoenix spacecraft was assembled 2011-2014

2009-2011

2010

Doctoral Dissertation Fellowship, University of Minnesota, Twin Cities Oral presentation award, North Central Branch-American Society for Microbiology

2006-2007 2006

MENTORING Antibiotic resistance Angelica Perez De La Rosa and Alexi Ernstoff: Graduate students at the University of Michigan Ecology of nitrifying bacterial communities in wastewater Andy Colby: Graduate student at the University of Michigan Wendell Khunjar: Postdoctoral fellow at the University of Michigan (presently postdoctoral fellow at Columbia University) Ameet Pinto: Graduate student at Virginia Tech (presently postdoctoral fellow at the University of Michigan) Extremophiles from NASA clean-rooms Ana Pearson: Universities Space Research Association Student Intern at the Jet Propulsion Laboratory (presently medical student at Indiana University) Biodegradation of carbadox in soil Pete Bly: Undergraduate student at the University of Minnesota

TEACHING Guest lecture Water and Wastewater Treatment (University of Minnesota) Biological Processes in Environmental Engineering (University of Michigan) Teaching assistant Grading, preparing solutions, holding office hours/advising students with experiments (Department of Civil Engineering, University of Minnesota) for Solid and Hazardous Waste (one semester), Remediation Technology (one semester), Introduction to Environmental Engineering (three semesters) and Experimental Methods in Environmental Engineering (one semester).

SERVICE Peer review Biochemical Engineering Journal, Environmental Microbiology, Environmental Science and Technology, Journal of Environmental Engineering, Journal of Environmental Management, Journal of Environmental Quality, Molecular Plant-Microbe Interactions Minority Mentoring Program in American Society for Microbiology

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Panelist in Women in Science and Engineering (WISE) discussion at the University of Michigan for graduate students interested in future postdoctoral research positions Secretary, environmental student group MEESE (Minnesota Environmental Engineers Scientists and Enthusiasts) at the University of Minnesota: 2006 Twin Cities public school science fair judge: 2002-2006

PROFESSIONAL DEVELOPMENT American Society for Microbiology Kadner Institute 2009: Workshop on careers in microbiology, grant writing and teaching Seminars at Center for Research on Learning and Teaching (University of Michigan) on Classroom Assessment Techniques and Active Learning Course on Teaching in Higher Education (University of Minnesota)

AFFILIATIONS American Society for Microbiology Association of Environmental Engineering and Science Professors

PEER-REVIEWED PUBLICATIONS 1. S. Ghosh, N. G. Love et al. Chlorophenols in disinfectants and antioxidants influence the antibiotic resistance profile of Pseudomonas aeruginosa. In preparation for Antimicrobial Agents and Chemotherapy. 2. S. Ghosh, C. M. Cremers, U. Jakob and N. G. Love. 2011. Chlorinated phenols control the expression of the multi-drug resistance efflux pump MexAB-OprM in Pseudomonas aeruginosa by activating NalC. Molecular Microbiology 79:1547-1556. 3. S. Ghosh and N. G. Love. 2011. Application of rbcL based molecular diversity analysis to algae in wastewater treatment plants. Bioresource Technology 102:3619-3622. 4. S. J. Ramsden, S. Ghosh, L. J. Bohl and T. M. LaPara. 2010. Phenotypic and genotypic analysis of bacteria isolated from three municipal wastewater treatment plants on tetracycline-amended and ciprofloxacin-amended growth media. Journal of Applied Microbiology 109: 1609-1618. 5. S. Ghosh, S. Osman, P. Vaishampayan and K. J. Venkateswaran. 2010. Recurrent isolation of extremotolerant bacteria from the clean room where Phoenix spacecraft components were assembled. Astrobiology 10: 325-335. 6. P. Vaishampayan, A. Probst, S. Krishnamurthi, S. Ghosh, S. Osman, A. McDowall, A. Ruckmani, S. Mayilraj and K. J. Venkateswaran. 2010. Bacillus horneckiae sp. nov., isolated from a clean room where the Phoenix spacecraft was assembled. International Journal of Systematic and Evolutionary Microbiology 60: 1031-1037.

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7. S. Ghosh, S. J. Ramsden and T. M. LaPara. 2009. The role of anaerobic digestion in controlling the release of tetracycline resistance genes and class 1 integrons from municipal wastewater treatment plants. Applied Microbiology and Biotechnology 84: 791-796. 8. S. Ghosh, M. J. Sadowsky, M. C. Roberts, J. A. Gralnick and T. M. LaPara. 2009. Sphingobacterium sp. strain PM2-P1-29 harbors a functional tet(X) gene encoding for the degradation of tetracycline. Journal of Applied Microbiology 106:1336-1342. 9. S. Ghosh and T. M. LaPara. 2007. The effects of subtherapeutic antibiotic use in farm animals on the proliferation and persistence of antibiotic resistance among soil bacteria. ISME Journal 1:191-203. 10. T. M. LaPara and S. Ghosh. 2006. Population dynamics of the ammonia-oxidizing bacteria in a full-scale municipal wastewater treatment facility. Environmental Engineering Science 23:309-319. 11. S. Ghosh and T. M. LaPara. 2004. Removal of carbonaceous and nitrogenous pollutants from a synthetic wastewater using a membrane-coupled bioreactor. Journal of Industrial Microbiology and Biotechnology 31:353-361.

CONFERENCES AND PRESENTATIONS 1. S. Ghosh, C. M. Cremers, U. Jakob, and N. G. Love. Gordon Research Conference on Environmental Sciences: Water. Holderness, New Hampshire. June 20-25, 2010. Chlorophenols modulate expression of the multidrug resistance efflux pump MexABOprM in Pseudomonas aeruginosa. 2. S. Ghosh and N. G. Love. American Association of Environmental Engineering and Science Professors. Iowa City, Iowa. July 26-29, 2009. Understanding mechanisms behind chlorophenol-induced increases in antibiotic resistance in Pseudomonas aeruginosa. 3. S. Ghosh and N. G. Love. American Society for Microbiology General Meeting. Philadelphia. Pennsylvania. May17-21, 2009. MexAB-OprM efflux pump mediated changes in antibiotic susceptibilities of Pseudomonas aeruginosa. 4. S. Osman, S. Ghosh, P. Vaishampayan and K. J. Venkateswaran. American Society for Microbiology General Meeting. Boston, Massachusetts. June 2-5, 2008. Molecular microbial burden and community analyses of Phoenix spacecraft assembly facility. 5. S. Ghosh. California Institute of Technology, Jet Propulsion Laboratory. Pasadena, California. September 6, 2007. Subtherapeutic antibiotic use and the proliferation of resistance among soil bacteria. 6. S. Ghosh and T. M. LaPara. American Society for Microbiology General Meeting. Toronto, Canada. May 21-25, 2007. Studying the proliferation of resistance in soil: cultivation vs. gene quantification. 7. Ghosh, S. North Central Branch-American Society for Microbiology. South Dakota State University, Brooking, South Dakota. Oct 19-20, 2006. Characterization of a tetracycline

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resistance conferring gene functional in its native host - Evidence of wider distribution and mobility of tet(X)? 8. S. Ghosh and T. M. LaPara. American Society for Microbiology General Meeting. Orlando, Florida. May 19-23, 2006. Distribution of integrons in different agricultural and nonagricultural soils and diversity of integrons in soil Exposed to waste from farm using subtherapeutic antibiotics in animal feed. 9. S. Ghosh. Dr. B C Guha Centre of Genetic Engineering and Biotechnology, University of Calcutta. Kolkata, India. February 2, 2006. Proliferation of antibiotic resistance: Does the environment serve as reservoir for resistant bacteria? 10. S. Ghosh. Institute of Post Graduate Medical Education and Research. Kolkata, India. January 27, 2006. Proliferation of antibiotic resistance: Does the environment serve as reservoir for resistant bacteria? 11. S. Ghosh, and T. M. LaPara. Conference on the Environment. Minneapolis, Minnesota. November 10, 2005. Impact of antibiotic use in agriculture on the proliferation of resistance among soil bacteria. 12. S. Ghosh. North Central Branch-American Society for Microbiology. Iowa State University, Ames, Iowa. September 22-24, 2005. Subtherapeutic antibiotic use in agriculture and the spread of resistance to soil bacteria. 13. S. Ghosh and T. M. LaPara. American Society for Microbiology General Meeting. Atlanta, Georgia. June 5-9, 2005. Impact of antibiotic use in agriculture on the proliferation of resistance among soil bacteria. 14. S. Ghosh, and T. M. LaPara. Conference on the Environment. Minneapolis, Minnesota. November 18, 2004. Antibiotic use in Agriculture: Its influence on the spread of antibiotic resistance. 15. S. Ghosh, and T. M. LaPara. North Central Branch-American Society for Microbiology. University of Wisconsin, Madison, Wisconsin. November 12-13, 2004. Antibiotic use in agriculture: Its influence on the spread of antibiotic resistance. 16. S. Ghosh. North Central Branch-American Society for Microbiology. University of Wisconsin, Oshkosh, Wisconsin. October 17-18, 2003. "Ammonia oxidizing bacterial communities from a full-scale wastewater treatment facility".

RESEARCH EXPERIENCE (1) Multidrug resistance and gene regulation in Pseudomonas aeruginosa Recently, I described the mechanism of regulation of the MexAB-OprM multidrug resistance (MDR) efflux pump in P. aeruginosa involving the transcriptional regulator NalC. Now I am looking at the role of the MDR efflux pump regulatory pathway and other cellular pathways in P. aeruginosa proliferation in undesirable niches such as drinking water supply lines and health-care facilities.

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(2) Algae in wastewater Like bacteria, algae form highly diverse consortia in water bodies. Molecular community analysis techniques are not as well developed for algae as they are for bacteria. I developed molecular techniques for analyzing algae communities and applied it to the study of algal diversity in wastewater treatment plants. One of my goals for looking at algae in wastewater treatment plants is to help inform the design of algae based wastewater treatment processes. (3) Extremophiles in clean-rooms My research at the Jet Propulsion Laboratory involved studying the ecology of bacteria tolerating extreme conditions, such as low nutrient levels and harsh cleaning regimens, in clean-rooms where spacecrafts are assembled. (4) Agricultural antibiotic use and proliferation of resistance As a graduate student, I investigated the role of animal waste management at farms using subtherapeutic antibiotics in spreading resistance among soil bacteria and the role played by horizontal gene transfer elements. My results show that current animal waste management practices have the potential to build a persistent reservoir of resistant bacteria in soil. (5) Antibiotic resistance in wastewater treatment plants A different project that I was involved in as a graduate student looked at the fate of the genes that confer antibiotic resistance and resistant bacteria through wastewater treatment processes. Our study of different wastewater treatment facilities saw that levels of several genes associated with antibiotic resistance went up in the sludge following mesophilic digestion.

LIST OF REFERENCES Prof. Nancy Love (Postdoctoral Adviser), Dept. of Civil and Environmental Engineering, University of Michigan; nglove@umich.edu, 734-764-8495 Prof. Tim LaPara (Graduate Adviser), Dept. of Civil Engineering, University of Minnesota; lapar001@umn.edu, 612-624-6028 Prof. Ursula Jakob, Molecular, Cellular, and Developmental Biology, University of Michigan; ujakob@umich.edu, 734-615-1957 Prof. Mike Sadowsky, Dept. of Soil, Water, and Climate, University of Minnesota; sadowsky@umn.edu, 612-624-2706 Prof. Jeff Gralnick, Dept. of Microbiology, University of Minnesota; gralnick@umn.edu, 612-626-6496

Sudeshna Ghosh, University of Michigan, Ann Arbor

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