mClavGR2 Photoconvertible Reporter Fusion Vectors

Box 1 | Product List Fluorescent Protein Vectors

Vector Name pmClavGR2-CT pmClavGR2-NT Cat. Number ABP-FP-CLAVC ABP-FP-CLAVN

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lleles photoconvertible proteins allow researchers to track the changes in localization in living cells by changing from green to red. These two fusion vectors are monomeric photoconvertible fluorescent expression vectors with multiple cloning sites for fusions to the C and N termini of mClavGR2. Compared with previously available photoconvertible FPs, mClavGR2 has improved photostability of the red state under confocal illumination conditions, 3644 over mEOS2s 2700 and Dendra2s 2420[1]. Most notable among other advantages of mClavGR2 is its monomeric structure, its highly optimized and rapid folding efficiency, and its superior photoconversion efficiency due to the high pKa of the green state.
[1] Hoi H, Shaner NC, Davidson MW, Cairo CW, Wang J, Campbell RE: A monomeric photoconvertible fluorescent protein for imaging of dynamic protein localization. J Mol Biol. 2010 Sep 3;401(5):776-91.

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pmClavGR2-CT (ABP-FP-CLAVC)
CMV Promoter ........ 1-589 Multiple Cloning Sites ........ 591-646 mClavGR2 ........ 659-1366 poly(A) Signal ........ 1397-1623 Ampicillin Resistance Gene ........ 2210-3070 pUC Origin ........ 3225-3844

Sequencing Primer: CMV-F and mClavGR2-R (ABP-FP-CLVRV)

Multiple Cloning Sites 587 647 707 1247 1307 ATCCGCTAGCTCTAGACTGGATCCTCGAATTCGGTACCTCAAGCTTGCGGCCGCCTCGAG NheI XbaI BamHI EcoRI KpnI HindIII NotI GCCACCATGGTGAGCAAGGGCGAGGAGACCATCATGAGCGTGATCAAGCCTGACATGAAG ATCAAGCTGCGCATGGAGGGCAACGTGAACGGCCACGCCTTCGTGATCGAGGGCGAGGGC >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> CACTTCGTGGACCACCGCATCGAGATCCTGAGCCACGACAAGGACTACAACAAGGTGAAG CTGTACGAGCACGCCGTGGCCCACAGCGGCCTGCCCGGCATGGACGAGCTGTACAAGTAA

pmClavGR2-NT (ABP-FP-CLAVN)
CMV Promoter ........ 1-589 mClavGR2 ........ 613-1323 Multiple Cloning Sites ........ 1324-1386 poly(A) Signal ........ 1428-1654 Ampicillin Resistance Gene ........ 2241-3101 pUC Origin ........ 3256-3875

Sequencing Primer: mClavGR2-F (ABP-FP-CLVFW)

Multiple Cloning Sites 607 667 1267 1327 1387 GCCACCATGGTGAGCAAGGGCGAGGAGACCATCATGAGCGTGATCAAGCCTGACATGAAG ATCAAGCTGCGCATGGAGGGCAACGTGAACGGCCACGCCTTCGTGATCGAGGGCGAGGGC >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> CTGTACGAGCACGCCGTGGCCCACAGCGGCCTGCCCGGCATGGACGAGCTGTACAAGTCC GGACTCAGATCTCGAGTCGGATCCTCGAATTCGGTACCCTCTAGACAAGCTTGCGGCCGC BglII XhoI BamHI EcoRI KpnI XbaI HindIII NotI TAA