APPLICATION: FOR GRANTINAID OF RESEARCH PROJECT Section A: GENERAL

Title of the Research ProjectMicrobial growth kinetics in peritoneal dialysis fluids and molecular techniques for diagnosis of culture negative peritonitis

Name and Designation of Investigators 1.Principal Investigator: Dr. N .Prasad, Assistant Professor, Department of Nephrology SGPGIMS, Lucknow-226014 2.Co-investigators: i) Dr. K.N.Prasad Professor, Department of Microbiology SGPGIMS, Lucknow-226014 2.Duration of Research Project: 3 Years i) ii) 2. Period which may be needed for collecting the data: 2 Years 9 months Period that may be required for analysing the data: 3 Months

Institution responsible for the research project: Name: Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Postal address: Raebareli Road, Lucknow- 226 014, India. Telephone: 2668700, 2668800 Telegraphic address: Ayurvigyan: Telex No. 0535 2411 SGPGI IS

1 . Title of the project: Microbial growth kinetics in peritoneal dialysis fluids and molecular techniques for diagnosis of culture negative peritonitis 2. Objectives of the study: 1- To study the growth kinetics of microbes in used and unused PD fluids and the factors influencing the growth. 2- PCR based detection of etiologic agents in culture negative CAPD peritonitis. 3Estimation of cytokines in PD fluid and serum in CAPD patients. 1a)Time kill effects, b) analysis of PD fluid, c) glucose degradation products 2- a) Universal PCR, b) differentiation between Gram +ve and ve infections, c) immune response

3. Summary of the proposed research.

Continuous ambulatory peritoneal dialysis (CAPD) is an established modality of treatment in patients with end stage renal disease (ESRD) and peritonitis is one of the frequent and serious complications in such patients. A variety of microbes are responsible for peritonitis in CAPD patients but their growth kinetics in CAPD fluids largely remains unknown. Hence Since peritonitis can be caused by a variety of orga nisms, we decided to determine the growth potential of common clinical isolates in CAPD fluid. .Infections and their complications result in substantial morbidity and frequently in death There is a need for early diagnosis of etiologic agent for targeted therapy. Broad range Gram specific PCR proposed in the study will not only help to identify the bacterial origin of infection but also differentiate the causative agent either Gram positive or Gram negative or mixed.Among dialysis patients immune cellular dysfunction may actually be responsible for the high incidence of infections in these patients .So, we planned to study the growth and survival potential of commonly isolated micro-organisms from patients with peritonitis in CAPD fluids and to study Gram-positive and Gram-negative infections to understand the pathogenesis of peritonitis by using gram specific PCR.

3 INTRODUCTION- Continuous ambulatory peritoneal dialysis (CAPD) was developed as an alternative treatment for end-stage renal disease[1]. The peritoneum serves as a dialysis membrane between blood and CAPD fluid. The most serious complication of CAPD is peritonitis. Since peritonitis can be caused by a variety of organisms, These included: CandidaAlbicans , Enterabacter sp., Escherichia cali , Enteracacci , Klebsiella pneumoniae, Prateus vulgaris , Pseudamonas aeruginosa , Pseudomanas sp., Serratia marcescens Staphylococcus aureus , S.epidermidis and Streptococcus viridans. Most episodes of peritonitis in CAPD patients are caused by Staphylacaccus epidermidis, S. aureus and Streptococcus sp. (2,3), thus implicating a cutaneous origin. Therefore, it seems that cutaneous gram-positive cocci are introduced into the peritoneal cavity during fluid changes.These organisms remain dormant until the fluid has changed enough chemically to allow their growth and subsequently peritonitis occurs. Endogenous sources of infection also occur. Transluminal migration of intestinal bacteria (Campylabacterjejuni) after alterations in the peritoneal or intestinal wall has been reported (4). The PD-fluids used in CAPD treatment have been assumed to be nontoxic in biological systems. As a consequence, minor attention has been paid to the chemically induced toxicity of these fluids. Instead,research has mainly focused on the adverse effects related to low pH and high osmolarity. Recently it was shown by Bronswijk et al [21 that PD-fluids can have acytotoxic influence on human mesothelial cells in vitro. Neither p11 exclusively, nor high glucose concentration, was suggested as the cause of the cytotoxicity found in that study. Furthermore,Topley et al [3] have suggested that PD-fluids with low pH may contain substances that inhibit inflammatory cells.Attention has also been drawn, by Henderson [4] to the fact thatPD-fluids could contain potentially harmful components as well as toxic contaminants. It was also pointed out that heat sterilization might induce decomposition and degradation of PDfluids.Since patients are treated several times daily and over many years, we believe that it would be of interest to test PD-fluids for general cytotoxicity. We conclude that the commercially available PD-fluids tested, regardless of brand, act cytotoxically on cultured fibroblasts.The toxicity might be related to the decomposition of the PD-fluids due to heat sterilization. Glucose is known to spontaneously decompose in various intermediates, for instance 5hydroxymethylfurfural, formicandlaevulinic acids. Furthermore, it has been suggested that the accumulation of glucose decomposition products may play a significant role in causing hypertonic infusion pain and that the degree of decomposition is related to both heat treatment and the age of the PD-fluid [4, 6]. However, all these degradation products have so far been assumed to be nontoxic It has been demonstrated that PD-fluids may suppress the production of superoxide anion from human neutrophils, The failure of peritoneal cells to react upon an inflammatory stimulus in the presence of PD-fluid might influence the cellular defence against bacterial infections. As some inflammatory cells in vitro regain their functions when the pH is neutralized [3], a possible explanation for the failure of the cells in peritoneum to react might be the low pH of the PD-fluid. In contrast, in vitro pre treatment of peripheral poiymorpho nuclear leukocytes (PMN) with an acidic buffer at pH5.3 did not suppress cellular responses [3], and acidic culturemedium (pH 5.5) was not toxic on mesothelial cells [2].

4 8. Detailed research plan Materials and methods: Study population: This prospective case- control study will be conducted in the Departments of Microbiology and Nephrology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow. Total 90 patients recruited for continuous ambulatory peritoneal dialysis (CAPD), and patients undergoing CAPD with history of recurrent / refractory peritonitis and an equal number of age and sex matched healthy controls will be enrolled for the study. Specimens: Blood- 4 ml (Total amount ) 1) In plain vial to separate sera for cytokine ELISA and stored at -80C till use Dialysate- whole drained bag 1) 50 ml for culture in case of suspected peritonitis 2) 2 ml in plan vial for cytokine ELISA and stored at -80C till use The growth kinetics of Escherichia coli, Enterococcus species, Staphylococcus aereus, S. epidermidis (coagulase negative Staphylococcus), Pseudomona aeruginosa and Candida species will be analyzed in sterile unused and used (dialysate), and infected (WBC > 100/dl) CAPD fluids. The above organisms will be chosen because they are the most common pathogens responsible for CAPD peritonitis and their growth kinetics differs markedly in different environmental conditions. The CAPD fluids will seeded with 2 x 104 CFU/ml of the above organisms and incubated at room temperature (RT) and 40 C, and cultured on a Muller Hinton agar at different time intervals (0, 4, 8 and 18 h) to quantify the viable organisms. Measurement of serum and dialysate cytokines: All sera from patients and controls will be collected and stored at -80C until use. Cytokines Will be estimated in sera and dialysate samples by sandwich ELISA using immunoassay kits according to the manufacturers instructions.

5 Molecular methods: DNA will be extracted from all culture negative samples using Qiagen DNA extraction kit (Qiagen, Company, Germany). The isolated DNA will be subjected to polymerase chain reaction (PCR) using universal bacteria specific primers (3, 4). PCR positive samples will be further subjected to Gram type specific primers for the differentiation of the etiologic agents into Gram positive and Gram negative (3, 4). The amplified products will be sequenced and subjected to blast search to identify agent at genus/ species level.

8. Facilities in equipment etc. available at the sponsoring Institution for the proposed investigation: Major equipments are available for carrying out the study at the sponsoring institute.
9. Budget requirements with detailed break-up and full justification Budget (in Rs.) 2 year 3rd year
nd

1 year Recurring: Culture Media including BACTEC Cytokine & ELISA kits Chemicals and primers for PCR/ RT-PCR, & sequencing Plastic & glassware Micropipettes Miscellaneous/travel etc. Total D Overhead expenses @15% of total budget. Total 25,000 1,00,000 90,000 25,000 25,000 25,000 2,90,000 43,500 3,33500

st

Total 75,000 3,00,000 2,10,000 75,000 25,000 75,000 7,60,000 1,20000 8,80000

25,000 1,00,000 60,000 25,000 25,000 2,35,000 35,250 2,70,250

25,000 1,00,000 60,000 25,000 25,000 2,35,000 35,250 2,70,250

Microbiological and molecular study of the collected samples

Collection of pus and MRI screening of the study subjects Recruitment and 0 Selection of patients 6 12 18 Time in months 24 30 36

Justification for Personnel expenses (Junior Research fellow): The JRF will conduct the

study, perform the various laboratory experiments, maintain and evaluate the data.
Justification for contingencies expenses: Various investigation costs are calculated based on the minimum charge schedule. Other contingency expenses are meant for other items and chemicals essential to carry out the project. Justification for MRI expenses: The presently working machine of MRI is on leased basis, and the charge is 4,000 per examination of which a major part goes to the leasing company. So we will have to pay for the MRI of subjects enrolled in study.

8 BIODATA OF INVESTIGATOR (S) 1. Name: Dr. K. N. Prasad 2. Designation: Professor, 3. Complete Postal Address: Dept. of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow- 226014; Mobile- 09415011548; Fax05222668017; email- knprasad@sgpgi.ac.in. 4. Date of Birth: March 31, 1954 5. Educational Qualification: MD in Microbiology (Banaras Hindu University) 6. Research /Training experience: Duration Feb84 to Jan87 March87 to Oct87 Oct87 to Dec89 Jan 89 to June93 July 93 to June 97 July97 to Till date Institution Institute of Medical Sciences (BHU) Do MGIMS (Sevagram, Wardha, M.S.) SGPGIMS SGPGIMS Do Nature of work done Senior Resident & Post Graduate Student Research Associate Lecturer Assistant Professor Associate Professor Additional Professor

7. Research specialization (Major Scientific fields of interest) Enteric pathogens with special reference to Helicobacter pylori and Campylobacter species. Central nervous system (CNS) infections with special reference to neuro-cysticercosis and brain abscess. Antibiotic resistance in nosocomial pathogens. Infections in AIDS, transplants and continuous ambulatory peritoneal dialysis patients

8. Important recent publications (last 5 years with titles and references), including papers in press Book Chapter 1. Prasad KN, Agarwal J. Bacteria in Text book- Atlas of Intestinal Infections in AIDS. Dionisio D (editor), Springer- Verlag Italia 2003; p 271-286. 2. Prasad KN. Diagnosis of peritonitis and exit site infection in patients on continous ambulatory peritoneal dialysis- in Indian Peritoneal Dialysis Practice Guidelines 2007; p 55-62. Publications in International

9 3. Sinha R, Agarwal A, Prasad KN, Misra R. Sporadic enteric reactive arthritis and undifferentiated spondyloarthropathy in Asian North Indians: Evidence for involvement of Salmonella typhimurium. J Rheumatol 2003; 30: 105-13. 4. Azad R, Gupta RK, Kumar S, Pandey CM, Prasad KN, Husain N, Husain M. Is neurocysticercosis a risk facfor in coexistent intracranial disease? An MRI based study. J Neurol Neurosurgery Psychiatry 2003; 74:359-61. 5. Garg M, Misra MK, Chawla S, Prasad KN, Roy R, Gupta RK. Broad identification of bacterial type from pus by IH MR spectroscopy. European J Clin Invest 2003; 33: 518-24. 6. Prasad N, Gupta A, Sharma RK, Prasad KN, Gulati S, Sharma AP. The spectrum of bacterial peritonitis in CAPD patients in a developing country- is it different? Perit Dial Int 2003; 23:400-02 7. Singh M, Prasad KN, Yachha SK, Krishnani N. Genotypes of Helicobacter pylori in children with upper abdominal pain. J Gastroenterol Hepatol 2003; 18: 1018-23. 8. Shivastava R, Upreti RK, Jain SR, Prasad KN, Seth P, Chaturvedi UC. Suboptimal chlorine treatment of drinking water leads to selection of multidrug-resistant Pseudomonas aeruginosa. Ecotoxicol Environ Safety 2004; 58:277-83. 9. Prasad KN, Prasad N, Gupta Amit, Sharma RK, Verma AK, Ayyagari A. Fungal peritonitis in patients on continuous ambulatory peritoneal dialysis: a single centre Indian experience. J Infect 2004; 48: 96-101. 10. Sinha S, Prasad KN, Pradhan S, Jain D, Jha S. Detection of preceding Campylobacter jejuni infection by PCR in patients with Gullain-Barre syndrome. Trans Roy Soc Trop Med Hyg 2004; 98: 342-6. 11. Garg M, Gupta RK, Husain M, Chawla S, Chawla J, Kumar R, Rao SB, Mishra MK, Prasad KN. Brain abscess: Etiological categorization of bacteria with in vivo proton MR spectroscopy. Radiology 2004; 230:519-527. 12. Ghoshal U, Prasad KN, Singh M, Tiwari DP, Ayyagari A. A comparative evaluation of phenotypic and molecular methods for the detection of oxacillin resistance in coagulase negative staphylococci. J Infect Chemotherp 2004; 10: 86-89 13. Kamra P, Azad R, Prasad KN, Jha S, Pradhan S, Gupta RK. Infectious meningitis: prospective evaluation with magnetization transfer MR imaging. Brit J Radiol 2004; 77:387-94. 14. Mishra AM, Gupta RK, Jaggi RS, Reddy JS, Jha DK, Husain N, Prasad KN, Bihari S, Husain M. Role of diffusion-weighted imaging and in vivo proton MR spectroscopy in the differential diagnosis of ring enhancing intracranial cystic mass lesions. J Comput Assist Tomogr 2004; 28: 540-7. 15. Gupta RK, Hasan K, Mishra AM, Jha D, Husain M, Prasad KN, Narayan PA. Increased fractional anisotropy in brain abscess versus other cystic intracranial lesions. Am J Neruroradiol 2005; 26:1107-14. 16. Jain D, Sinha S, Prasad KN, Pandey CM. Campylobacter species and drug resistance in a north Indian rural community. Trans Roy Soc Trop Med Hyg 2005; 99: 207-14. 17. Gupta RK, M Prakash, Mishra AM, Husain M, Prasad KN, Husain N. Role of diffusion weighted imaging in differentiation of intracranial tuberculoma and tuberculous abscess from cystic granulomas-a report on more than 100 lesions. European J Radiol 2005; 55:384-92.

10 18. Mishra AM, Gupta RK, Saksena S, Prasad KN, Pandey CM, Rathore D, Purwar A, Rathore RKS, Husain N, Jha DK, Jaggi RS, Husain M. Biological Correlates of Diffusivity in Brain Abscess. Magn Reson Med 2005; 54:878-85. 19. Singh M, Prasad KN, Saxena A, Krishnani N, Yachha SK. Helicobacter pylori infection in children: a direct assessment from gastric biopsies. Trans Roy Soc Trop Med Hyg 2006; 100: 227-33. 20. Prasad KN, Chawla S, Prasad A, Tripathi M, Husain N and Gupta RK. Clinical signs for identification of neurocysticercosis in swine naturally infected with Taenia solium. Parasitol Int 2006; 55:151-4. 21. Singh M, Prasad KN, Saxena Ashish andYachha SK. Helicobacter pylori induces apoptosis of T- and B-Cell lines and translocates mitochondrial apoptosis-inducing factor to nucleus 2006;52:254-60. 22. Singh M, Prasad KN, Krishnani Narendra, Saxena Ashish and Yachha SK. Helicobacter pylori infection, histopathological gastritis and gastric epithelial cell apoptosis in children. Acta Pediatrica 2006; 95: 732-7. 23. Prasad KN, Mishra AM, Gupta D, Husain N, Husain M, Gupta RK. Analysis of microbial etiology and mortality in patients with brain abscess. J Infect 2006; 53:221-7. 24. Mishra AM, Reddy SJ, Husain M, Behari S, Husain N, Prasad KN, Kumar S, Gupta RK. Correlation of magnetization transfer ratio and fluid-attenuated inversion recovery imaging signal intensity with biological parameters in cystic intracranial mass lesions. J Magn Reson Imaging 2006; 24:52-6. 25. Trivedi R, Gupta RK, Hasan KM, Ho P, Prasad KN, Narayana PA. Diffusion tensor imaging in polymicrogyria- a report of three cases. Neuroradiology 2006; 48:422-7 26. Sinha S, Prasad KN, Jain D, Pandey CM, Jha S, Pradhan S. Preceding infections and anti-ganglioside antibodies in patients with Guillain-Barr syndrome: a single center prospective case-control study. Clin Microbiol Infect 2007; 13: 334-7. 27. Gupta RK, Haris M, Husain N, Husain M, Prasad KN, Pauliah M et al. Relative Cerebral Blood Volume is a Measure of Angiogenesis in Brain Tuberculoma. J Comput Assist Tomogr 2007; 31: 335-41. 28. Trivedi R, Malik GK, Gupta RK, Gupta A, Nath K, Prasad KN et al. Increased anisotropy in neonatal meningitis: an indicator of meningeal inflammation. Neuroradiology 2007; 49:767-75. 29. Prasad KN, Prasad A, Gupta RK, Pandey CM, Singh U. Prevalence and associated risk factors of Taenia solium taeniasis in a rural pig farming community of North India. Trans Roy Soc Trop Med Hyg 2007; 101: 1241-7 30. Husain N, Sharma S, Verma R, Shukla N, Gupta RK, Prasad KN. Multimodal Diagnostic Approach to Brain Abscess: Internet J Trop Med. 2007; 3: 1-6. 31. Luthra G, Parihar A, Nath K, Jaiswal S, Prasad KN, Husain N et al. Comparative evaluation of fungal, tubercular and pyogenic brai abscesses with convention, diffusion MR imaging and proton MR spectroascopy. Am J Neuroradiol 2007; 28:1332-8. 32. Gupta RK, Haris M, Husain N, Prasad KN et al. Relative cerebral blood volume is a measure of angiogenesis in brain tuberculoma. J Comp Assist Tomogr 2007:31; 355-41.

11 33. Nath K, Husain M, Trivedi R, Kumar Raj, Prasad KN, Rathore RKS, Gupta RK. Clinical implications of increased fractional anisotropy in meningitis associated with brain abscess. J Comput Assist Tomogr 2007; 3:888-893. 34. Malik GK, Trivedi R, Gupta A, Singh R, Prasad KN, Gupta RK. Quantitative DTI assessment of periventricular white matter changes in neonatal meningitis. Brain Dev 2007 (in press) 35. Gupta RK, Nath K, Prasad A, Prasad KN, Husain M, Rathore RKS et al. In vivo demonstration of neuroinflammatory molecule expression in brain abscess with diffusion tensor imaging. Am J Neuroradiol 2007; 29: 326-32. 36. Kumar M, Gupta RK, Nath K, Rathore RKS, Bayu G, Trivedi R, Husain M, Prasad KN, Tripathi R P, Narayana P A. Can We Differentiate True White Matter Fibers from Pseudofibers inside Brain Abscess Cavity using Geometrical DTI metrics? NMR BioMed 2007 Nov 30: (in press). 37. Prasad A, Prasad KN, Gupta RK. Nath K, Pradhan S, Tripathi M, Pandey CM. Neurocysticercosis in Patients with Active epilepsy from Pig Farming Community of Lucknow District, North India. Trans Roy Soc Trop Med Hyg 2008 (accepted). 38. Prasad A, Gupta RK. Nath K, Pradhan S, Tripathi M, Pandey CM, Prasad KN. What Triggers Seizures in Neurocysticercosis?- A MRI Based Study in Pig Farming Community from a District of North India. Parasitol Int 2008 (in press). 39. Prasad A, Prasad KN, Yadav A, Gupta RK, Pradhan S, Jha S, Tripathi M, Husain M. Lymphocyte transformation test: a new method for diagnosis of neurocystierosis. Diagn Microbiol Infect Dis 2008 (accepted) 40. Jain D, Prasad, KN, Sinha S. and Husain N. Differences in virulence attributes between cytolethal distending toxin positive and negative Campylobacter jejuni strains J Med Microbiol, 2007(in press, DOI 10.1099/jmm.0.47317-0) 41. Saxena A, Prasad KN, Ghoshal UC, Bhagat MR, Krishnanai N, Husain N. Polymorphism of -765G > C COX-2 is a risk factor for gastric adenocarcinoma and peptic ulcer disease in addition to Helicobacter pylori infection: A study from North India. World J Gastroenterol 2008 (accepted) 42. Saxena A, Prasad KN, Ghoshal UC, Bhagat MR, Krishnanai N, Husain N. Association of Helicobacter pylori and Epstein-Barr virus with gastric cancer and peptic ulcer disease. Scand J Gastroenterol 2008 (accepted) 43. Singh M, Prasad KN, Yachha SK. Elongated Helicobacter pylori in gastric mucosa of children associated with gastric disease. J Ped Infect Dis; 2008 (in press) 44. Prasad KN, Saxena A, Ghoshal UC, Bhagat MR, Krishnanai N. Analysis of Pro12Ala PPAR gamma polymorphism and Helicobacter pylori infection in gastric adenocarcinoma and peptic ulcer disease. Annals Oncol 2008 (in press). Publications in National Journals 45. Prasad KN, Agarwal J, Nag VL, Verma AK, Dixit AK, Ayyagari A. Cryptococcal infection in patients with clinically diagnosed meningitis in a tertiary care centre. Neurology India 2003; 51: 354-6. 46. Verma AK, Prasad KN, Singh M, Dixit AK, Ayyagari A. Candidaemia in patients of a tertiary care hospital from India. Indian J Med Res 2003; 17:122-128.

12 47. Wakhlu A, Agarwal V, Dabadghao S, Prasad KN, Nityanand S. Nocardiosis in patients of chronic idiopathic thrombocytopenic purpura on steroids: a report of two cases. J Assoc Physicians India 2004; 52:591-3. Total Publications: 101 (International- 61, National- 40) 9. Financial support received Title of project 1. From ICMR Past 1. Campylobacter infection and GuillainBarre syndrome as Principal Investigator 2. Magnitude of intestinal taeniasis and systemic cysticercosis in pig farming community of Lucknow District 3. Role of antiganglioside antibodies and IgG Fcreceptor (Fc R) polymorphisms in development of Campylobacter related Guillain-Barre syndrome 1. Prevalence of neurocysticercosis in asymptomatic rural pig farming community from high endemic area 2. Immunopathogenesis of Campylobacter jejuni induced paralysis resembling GuillainBarr syndrome in chickens Helicobacter pylori in children: its prevalence, role as pathogen and genotypic characterization Role of cell cycle regulatory genes in the pathogenesis of Helicobacter pylori infection Total amount of grant Rs 2, 22,800.00 per annum Rs. 3,94,580.00 Per annum Total period of support with date Three years; February, 2001 Three years; May 2003

Rs. 1,54,500.00

One year; February 2005

Present

Rs. 9,20,511.00 per annum

Three years; November 2007

Rs. 4,29,540.00 per annum Rs. 2,72,000.00

Three years; November 2007 Two years; August, 2001 Two years; May, 2007

2. From other sources (UP-CST) Past Present

Rs 1,70,000.00 per annum

13

Rakesh K. Gupta
Department of Radiodiagnosis Sanjay Gandhi Postgraduate Institute of Medical Sciences Raebareli Road, Lucknow-226014 India Tel: 91 (0522) 2668700, 2668900, Ext: 2599 (Off), 2600 (Resi) Fax: 91 (0522) 2668017, 2668973 Telex: 535-411-SGPG-IN

Marital status : Married

Telegram: AYURVIGYAN
E-mail: rgupta@sgpgi.ac.in : rakeshree@hotmail.com

Nationality Date of Birth

: Indian : August 22, 1955

Place of Birth : Delhi, India M.B.B.S. Delhi University 1978 1983 Medical Officer

M.D. (Radio-diagnosis) Delhi University April 83 - July 84

Hindu Rao Hospital, Delhi

Aug 84 - Oct 89 Oct 89 - June 94

INMAS, Delhi SGPGIMS, Lucknow

Scientist C Associate Professor Additional Professor Professor

July 94 -June 2003 SGPGIMS, Lucknow July 2003- till date SGPGIMS, Lucknow

Research Grants 1. Intramural PI: Central nervous system appearance in congenital sporadic hypothyroidism and endemic iodine deficiency cretins on MRI. (SGPGIMS, Lucknow) Grant completed. 2. Extramural PI: Ex vivo, in vitro, in vivo proton MR spectroscopy of different stages of neurocysticercosis of humans and animals. (DST, New Delhi). Grant completed. 3. Extramural PI: Finger printing of causative bacteria in brain abscess using different magnetic resonance techniques in humans and animal model. (ICMR, New Delhi) Grant in progress. 4. Extramural PI: Quantification of angiogenesis in brain tuberculomas with dynamic contrast MRI. (DST, New Delhi) Grant started. 5. Exramural PI: understanding fetal brain development through DTI with histologic correlation. Sanctioned by DBT. List of publications (2004 onwards): 1. Husain M, Jha D, Thaman D, Husain N, Gupta RK. Ventriculostomy in a tumor involving the third ventricular floor. Neurosurg Review; 27(1):70-72; 2004.

14 2. Chawla S, Gupta RK, Husain N, Kumar R, Garg M, Kumar S. Prediction of viability of neurocysticercosis with proton magnetic resonance spectroscopy and its correlation with histopathology. Life Science 16;74(9):1081-1092; 2004. 3. Garg M, Gupta RK, Husain M, Chawla S, Chawla J, Kumar R, Rao SB, Misra MK, Prasad KN. Brain abscess: etiological categorization of with in vivo proton MR spectroscopy. Radiology 230: 519-527; 2004. 4. Kamra P, Azad R, Prasad KN, Jha S, Pradhan S, Gupta RK. Infectious meningitis: prospective evaluation with magnetization transfer MR imaging. Brit J. Radiol. 77:387-394; 2004. 5. Agarwal A, Raghav S, Husain M, Kumar R, Gupta RK. Epilepsy with focal cerebral calcification: Role of magnetization transfer MR imaging. Neurology India. 52:197-199:2004. 6. Chawla S, Kumar S, Roy R, Garg M, Kumar R, Gupta RK. Metabolite pattern of cysticercus cyst in different tissue locations of swine using proton MR spectroscopy. Parasite 11:161-167;2004. 7. Kumar A, Gupta RK, Thomas MA, Alger JR, Wyckoff N, Hwang S. Biophysical changes in normal appearing white matter and subcortical nuclei in late-life major depression detected using magnetization transfer. Psychiatric Res 15; 130(2):13140; 2004. 8. Agarwal M, Chawla S, Husain N, Jaggi RS, Husain M, Gupta RK. Succinate more than acetate differentiates degenerating cysticercus cysts from anaerobic abscesses in brain on in vivo proton MR spectroscopy. Neuroradiology 46:211215; 2004. 9. Chawla S, Husain N, Kumar S, Pal L, Tripathi M, Gupta RK. Correlative MR imaging and histopathology in porcine neurocysticercosis. J Magn Reson Imaging; 20(2):208-15;2004. 10. Mishra AM, Gupta RK, Jaggi RS, Reddy JS, Jha DK, Husain N, Prasad KN, Behari S, Husain M. Role of diffusion-weighted imaging and in vivo proton MR spectroscopy in the differential diagnosis of ring enhancing intracranial cystic mass lesions. J Comput Assist Tomogr 28:540-547; 2004. 11. Prakash M, Gupta RK, Jha D, Mishra AM, Jaggi RS, Husain M. Brain abscess: MRI demonstration of rare cause of Intraventricular Hemorrhage. European Journal of Radiology 51: 9193; 2004. 12. Rajasekar D, Datta NR, Gupta RK, Rao SB. A graphical user interface for automatic image registration software designed for radiotherapy treatment planning. Medical Dosimetry 29:239-246; 2004. 13. Prakash M, Kumar S, Gupta RK. Diffusion-weighted MR imaging in Japanese encephalitis. J Comput Assist Tomogr 28:756-761, 2004. 14. Deepshikha S, Gupta RK. Magnetic resonance imaging in medicine. Proc Indian National Sci Acad 70:547-554, 2004. 15. Chawla S, S Kumar, Gupta RK. Marker of parasitic cysts on in vivo proton magnetic resonance spectroscopy: is it succinate or pyruvate? J Magn Reson Imaging 20: 1052-53, 2004. 16. J Kishore, Pradhan S, Gupta RK. Neurocysticercosis in clinically suspected and MRI proven cases. Indian J Pathol Microbiol 47(2):290-294, 2004. 17. Jaggi RS, Husain M, Chawla S, Gupta A, Gupta RK. Diagnosis of bacterial cerebellitis: diffusion weighted imaging and proton MR spectroscopy. Pediatric Neurol 32:72-74, 2005.

15 18. Gupta RK, Rao AM, Mishra AM, Chawla S, Raja Sekar D, Venkatesan R. Diffusion-weighted EPI with magnetization transfer contrast. Magn Reson Imaging 23:35-39, 2005. 19. Binesh N, Huda A, Bugbee M, Gupta RK, Rasgon N, Kumar A, Green M, Han S, Thomas MA. Adding another spectral dimension to 1H MR spectroscopy of hepatic encephalopathy. J Magn Reson Imaging 21:398-405, 2005. 20. Husain M, Jha DK, Agarwal S, Husain N, Gupta RK. Conical working tube: a special device for endoscopy surgery of herniated lumbar disc. J Neurosurg Spine 2:265-270, 2005. 21. Gupta RK, Hasan KM, Mishra AM, Jha D, Husain M, Prasad KN, Narayana PA. High fractional anisotropy in brain abscesses versus other cystic intracranial lesions Am J Neuroradiol 26:1107-1114, 2005. 22. Himmelreich U, Accurso R, Malik R, Dolenko B, Somorjai RL, Gupta RK, Gomes L, Mountford CE, Sorrell TC. Identification of Staphylococcus aureus brain abscesses: rat and human studies with 1H MR spectroscopy. Radiology 236(1):261-170, 2005. 23. Saxena S, Prakash M, Kumar S, Gupta RK. Comparative evaluation of magnetization transfer contrast and fluid attenuated inversion recovery sequences in brain tuberculoma. Clinical Radiology 60:787-793, 2005. 24. Hasan KM, Gupta RK, Kanabar BP, Santos RM, Wolinsky JS, Narayana PA. Diffusion tensor fractional anisotropy of the normal appearing seven segments of corpus callosum in healthy adults and relapsing- remitting MS patients. J Magn Reson Imaging 21(6): 735-43, 2005. 25. Gupta RK, Agarwal A, Agarwal S, Mohan P, Husain M, Alger JR, Pandey CM, Datta D. Reversal of acute human brain ischemic injury by lysine induced therapeutic angiogenesis: a pilot study. Internet J Neurology 4(1): 2005. 26. Gupta RK, Hasan KM, Trivedi R, Pradhan M, Das V. Narayana PA. Noninvasive demonstration of neuronal migration and cortical maturation in developing human cerebrum with diffusion tensor imaging. J Neuroscience Res 81(2):172-178, 2005. 27. Husain M, Jha D, Rastogi M, Husain N, Gupta RK. Role of neuroendoscopy in the management of patients with tuberculous meningitis hydrocephalus. Neurosurg Review 28(4):278-283, 2005. 28. Gupta RK, Prakash M, Mishra AM, Husain M, Prasad KN, Husain N. Diffusion weighted imaging of intracranial tuberculoma and tuberculous abscess. European J Radiology 55(3): 384-392, 2005. 29. Mishra AM, Gupta RK, Saksena S, Prasad KN, Pandey CM, Rathore D, Purwar A, Rathore RKS, Husain N, Jha DK, Jaggi RS, Husain M. Biological correlates of diffusivity in brain abscess. Magn Reson Med. 54:878-885, 2005. 30. Gupta RK, Saksena S, Agarwal A, Hasan KM, Husain M, Gupta V, Narayana PA. Diffusion tensor imaging in late posttraumatic epilepsy. Epilepsia 46(9):1465-1471, 2005. 31. P Ranjan, Mishra AM, Kale R, Saraswat VA, Gupta RK. Cytotoxic edema is responsible for raised intra-cranial pressure in fulminant hepatic failure: in-vivo demonstration using diffusion weighted MRI in human subjects. Metabolic Brain Diseases 20(5);181-192, 2005. 32. Rathore RKS, Gupta RK, K Raman, Rathore DKS. Fourier trigonometric compression in magnetic resonance imaging. International Journal of Tomography & Statistics. 3; (JJ05) 44-80, 2005.

16 33. Tripathi M, Nath SS, Gupta RK. Paraplegia following intracord injection during attempted epidural steroid injection in the awake patient. Anesthesia Analgesia 101:1209-1211, 2005. 34. Shukla R, P Vinod, S Prakash, Phadke RV, Gupta RK. Subarachnoid haemorrhage as a presentation of cerebral venous sinus thrombosis. J Assoc Physicians India 54: 42-44, 2006. 35. Datta S, Rao SB, He R, Wolinsky JS, Gupta RK, Narayana PA. Segmentation and quantification of black holes in multiple sclerosis. Neuroimage 29:467-474, 2006. 36. Kale RA, Gupta RK, Saraswat VA, Hasan KM, Trivedi R, Mishra AM, P Ranjan, Pandey CM, Narayana PA. Demonstration of interstitial cerebral edema with diffusion tensor MR imaging in type C hepatic encephalopathy. Hepatology 43:698-706, 2006. 37. Prasad KN, Mishra AM, Gupta D, Husain N, Husain M, Gupta RK. Analysis of microbial etiology and mortality in patients with brain abscess. J Infect. 2006; 53(4):221-7. 38. Agarwal S, Nag P, Sikora S, Prasad TL, Kumar S, Gupta RK. Fentanylaugmented MRCP. Abdom Imaging 2006 Sep-Oct;31(5):582-7 39. Prasad KN, Chawla S, Prasad A, Tripathi M, Husain N, Gupta RK. Clinical signs for identification of neurocysticercosis in swine naturally infected with Taenia solium. Parasitol Int 2006 Jun;55(2):151-4. 40. Trivedi R, Gupta RK, Hasan KM, Hou P, Prasad KN, Narayana PA. Diffusion tensor imaging in polymicrogyria- a report of three cases. Neuroradiology 2006 Jun;48(6):422-7. 41. Rao SB, Datta S, He R, Mehta M, Gupta RK, Wolinsky JS, Narayana PA. Unified approach for multiple sclerosis lesion segmentation on brain MRI. Ann Biomed Eng 2006;34(1):142-51. 42. Rajan Syal, Raj Kumar, Jain VK, Mishra AM, Gupta RK. Unusual clinical and MRI features of a cerebellopontine angle medullo epithelioma: case report and review of literature. Pediatr Neurosurg 2006;42(5):299-303. 43. Trivedi R, Gupta RK, Agarawal A, Hasan KM, Getaneh Bayu, Divya Rathore, Ram Kishore S Rathore, Narayana PA. Assessment of white matter damage in subacute sclerosing panencephalitis using quantitative diffusion tensor MRI. Am J Neuroradiol 2006;27(8):1712-6. 44. Mishra AM, Reddy SJ, Husain M, Behari S, Husain N, Prasad KN, Kumar S, Gupta RK. Correlation of magnetization transfer ratio and fluid-attenuated inversion recovery imaging signal intensity with biological parameters in cystic intracranial mass lesions. J Magn Reson Imaging 2006 Jun 9;24(1):52-56. 45. Reddy JS, Mishra AM, Behari S, Husain M, Rastogi M, Gupta RK. Role of diffusion-weighted imaging in the differential diagnosis of intracranial cystic mass lesions: a report of 147 lesions. Surgical Neurology 2006 Sep;66(3):246-50. 46. Gupta RK, Saksena S, Hasan KM, Agarwal A, Haris M, Pandey CM, Narayana PA. Focal wallerian degeneration of corpus callosum in large middle cerebral stroke: serial diffusion tensor imaging. J Magn Reson Imaging 2006;24 (3):549555. 47. Haris M, Gupta RK, Husain N, Hasan KM, Husain M, Narayana PN. Measurement of DTI metrics in hemorrhagic brain lesions: possible implication in MRI interpretation. JMRI 2006;24(6):1259-68.

17 48. Verma A, Gupta RK, Kumar S, Mohan S, Baijal SS. Non-invasive imaging of persistent primitive hepatic venous plexus using Doppler sonography and magnetic resonance imaging. European Journal of Radiology 60:69-73, 2006. Malik GK, Trivedi R, Gupta RK, Hasan KM, Hasan Mu, Gupta A, CM Pandey,. Narayana PA. Serial Quantitative Diffusion Tensor MRI of the Term Neonates with Hypoxic-Ischemic Encephalopathy (HIE). Neuropediatrics 2006; 37:337-43. 50. Gupta Arun, Malik GK, Gupta Amit, Saksena Sona, Gupta RK. MRI in complete cerebellar and corpus callosum agenesis. Pediatric Neurosurgery 2007; 43:29-31. 51. Tripathi M, Nath SS, Kumar V, Gupta RK. Magnetic resonance imaging helped detect the precise cause of post intubation hoarseness presenting as vocal cord paralysis. Anaesth Analg. 2007; 104:470-1. 52. Rumboldt Z, Thurnher MM, Gupta RK. Central nervous system infections. Seminars in Roentgenol 2007; 42:62-91 53. Srivastava AK, Gupta RK, Haris M, Ray M, Kalita J, Misra UK. Cerebral venous sinus thrombosis: Developing an experimental model. J Neurosci Methods. 2007 Apr 15; 161(2):220-2 54. Husain M, Rastogi M, Jha DK, Husain N, Gupta RK. Endoscopic transaqueductal removal of fourth ventricular neurocysticercosis using angiographic catheter. Neurosurgery 2007 Apr;60(4 Suppl 2):249-53. 55. Datta S, Sajja BR, He R, Gupta RK, Wolinsky JS, and Narayana PA. Segmentation of gadolinium enhanced lesions on MRI in multiple sclerosis. J Magn Reson Imaging 2007 May;25(5):932-7. 56. Husain M, Jha D, Rastogi M, Husain N, Gupta RK. Neuro-endoscopic management of intraventricular neurocysticercosis. Acta Neurochirurgica (Wien). 2007; 149:341-346. 57. Pauliah M, Saxena V, Haris M, Husain N, Rathore RKS, Gupta RK. Improved T1-Weighted Dynamic Contrast Enhanced MRI to probe microvascularity and heterogeneity of human glioma. Magn Reson Imaging 2007 May 7; [Epub ahead of print] 58. Datta S, Sajja BR, He R, Gupta RK, Wolinsky JS, Narayana PA. Segmentation of gadolinium-enhanced lesions on MRI in multiple sclerosis. J Magn Reson Imaging. 2007; 25:932-7. 59. Gupta RK, Haris M, Husain N, Husain M, Prasad KN, Pauliah M, Srivastava C, Tripathi M, Rastogi M, Behari S, Rathore D, Rathore RKS. Relative cerebral blood volume is a measure of angiogenesis in brain tuberculoma and its therapeutic implications. JCAT 2007; 31:335-341. 60. Prasad N, Gulati S, Gupta RK, Sharma K, Gulati K, Sharma RK, Gupta A. Spectrum of radiological changes in hypertensive children with reversible posterior leucoencephalopathy. Br J Radiol. 2007; 80:422-9. Epub 2007 Mar 28. 61. Husain N, Sharma S, Verma R, Shukla N, Gupta RK, Prasad KN, Husain M. Multimodal Diagnostic Approach to Brain Abscess. The Internet Journal of Tropical Medicine. 2007. Volume 3 Number 2. 62. Luthra G, Parihar A , Nath K , Jaiswal S , Prasad KN, Husain N, Husain M, Singh S, Behari S, Gupta RK Comparative Evaluation of Fungal, Tubercular and Pyogenic Brain Abscesses with Conventional, Diffusion MR Imaging and Proton MR Spectroscopy. American Journal of Neuroradiology 2007; 28:1332-8. 49.

18 63. Trivedi R., Malik G.K., Gupta R.K., Gupta A., Nath K., Prasad K.N., Purwar A., Rathore D, Rathore R.K.S., Narayana P.A.. Increased cortical anisotropy in Neonatal Meningitis-An indicator of meningeal inflammation. Neuroradiology 2007; 49: 767-775. 64. Jain KK, Mittal SK, Kumar S, Gupta RK. Imaging Features of CNS Fungal Infections. Neurology India 2007; 55:241-50. 65. Prasad KN, Prasad A, Gupta RK, Pandey CM and Singh U. Prevalence and associated risk factors of Taenia solium taeniasis in a rural pig forming community of North India. Transactions of Royal Society of Tropical Med and Hygiene.2007, 101:1241-1247. 66. Singh A, Haris Md, Rathore DKS, Purwar A, Sarma MK, Bayu G, Husain N, Rathore RKS, Gupta RK. Quantification of physiological (endothelial permeability, fractional volume of leaky space) and hemodynamic (cerebral blood flow and cerebral blood volume) indices using T1 dynamic contrast enhanced MRI in intracranial mass lesions. JMRI 2007; 26:871-80. 67. Saksena S, Rai V, Saraswat VA, Rathore RKS, Purwar A, Kumar M, Thomas MA, Gupta RK. Diffusion Tensor Imaging and In Vivo Proton MR Spectroscopy in Patients with Fulminant Hepatic Failure. Journal of Gastroenterology and Hepatology 2007 Oct 9; [Epub ahead of print]. 68. Tripathi M, Chawla S, Gupta RK. Propofol infusion anaesthesia for MRI in swine. Ind Vet Journal 2007; 84:932-935. 69. Nath Kavindra, Husain Mazhar, Trivedi Richa, Kumar Raj, Prasad Kashi N, Rathore RKS, Gupta RK, Clinical Implications of Increased Fractional Anisotropy in Meningitis associated with Brain Abscess. JCAT 2007 November/December;31(6):888-893 70. Poonawalla AH, Hasan KM, Gupta RK, Nelson FM, Ahn CW, Wolinsky JS, Narayana PA Diffusion Tensor Imaging of Cortical Lesions in Multiple Sclerosis. Radiology 2008 Jan 14; [Epub ahead of print] 71. Gupta RK, Nath Kavindra, Prasad Amit, Prasad Kashi N, Husain Mazhar, Rathore RK.S, Husain Nuzhat, Srivastava Chhitiz, Khetan Prakash, Trivedi Richa, Narayana Ponnada A. In vivo Demonstration of Neuroinflammatory Molecules Expression in Brain Abscess with Diffusion Tensor Imaging. AJNR 2007 Nov 7; [Epub ahead of print]. 72. Husain N, Shukla N, Kumar R, Husain M, Chaturvedi Arun, Agarwal Girdhar G, Gupta RK. ELISA in the evaluation of therapeutic response to albendazole in neurocysticercosis. Journal of Infection 2007 Nov 5; [Epub ahead of print]. 73. Kumar M, Gupta RK, Nath K, Rathore RKS, Bayu2 G, Trivedi R, Husain M, Prasad KN, Tripathi RP, Narayana PA. Can We Differentiate True White Matter Fibers from Pseudofibers inside Brain Abscess Cavity using Geometrical DTI metrics? NMR In Biomed 2007 Nov 30; [Epub ahead of print] 74. Malik GK, Trivedi R, Gupta A, Singh R, Prasad KN, Gupta RK. Quantitative DTI Assessment of Periventricular White Matter changes in Neonatal Meningitis. Brain & Development 2007 Nov 12; [Epub ahead of print] 75. Prasad A, Gupta RK, Pradhan S, Tripathi M, Luthara G, Pandey CM, Prasad KN. What triggers seizures in neurocysticercosis? - A MRI based study in pig farming community from a district of North India. Parasitology International. 2007,Dec 8; [Epub ahead of print]

19 Haris M, Gupta RK, Husain M, Srivastava C, Singh A, Rathore RKS, Saksena S, Behari S, Husain N, Pandey CM, Prasad KN. Assessment of Therapeutic Response on Serial Dynamic Contrast Enhanced MR Imaging in Brain Tuberculomas. Clinical Radiology (in press). 77. Singh I, Haris M, Husain M, Husain N, Rastogi M, Gupta RK. Role of Endoscopic Third Ventriculostomy in Patients with communicating Hydrocephalus- An Evaluation by MR Ventriculography. Neurosurg Review (in press). 78. Verma A, Saraswat VA, Radha Krishna Y, Nath K, Thomas MA, Gupta RK. Role of 1H-MR Spectroscopy in the Differentiation of Acute on Chronic Liver Failure from Acute Liver Failure. Liver International (in press). 79. Gupta RK, Jain KK, Kumar S. Imaging of non-specific (non-herpetic) acute viral infections. Neuroimaging Clin North Am (in press). 80. Husain N, Awasthi S, Haris M, Gupta RK, Husain M. Vascular endothelial growth factor as a marker of disease activity in neurotuberculosis. J Infect 2007 Dec 22 [Epub ahead of print]. 81. Haris M, Kumar S, Raj MK, Das KJM, Sapru S, Behari S, Rathore RKS, Narayana PA, Gupta RK. Serial diffusion tensor imaging to characterize radiation induced changes in normal appearing white matter following radiotherapy in patients with adult lowgrade gliomas. Radiation Medicine (in press). 82. Haris M, Husain N, Singh A, Husain M, Srivastava S, Srivastava C, Behari S, Rathore RKS, Saksena S, Gupta RK. Dynamic contrast enhanced derived cerebral blood volume correlates better with leak correction than with no correction for vascular endothelial growth factor, micro vascular density and grading of astrocytoma. JCAT (in press). 83. Guleria S, Gupta RK, Saksena S, Chandra A, Srivastava RN, Husain M, Rathore RKS, Narayana PA. Retrograde wallerian degeneration of cranial corticospinal tracts in cervical spinal cord injury patients using diffusion tensor imaging. J Neuroscience Research (in press). 84. Nath K, Saraswat VA, Krishna YA, Thomas MA, Rathore RKS, Pandey CM, Gupta RK. Quantification of cerebral edema on diffusion tensor imaging in acute-on-chronic liver failure due to acute hepatitis A or E superimposed on liver cirrhosis. NMR in Biomed (in press). 85. Prasad A, Prasad KN, Gupta RK, Nath K, Pradhan S, Tripathi M, Pandey CM. Neurocysticercosis in patients with active epilepsy from pig farming community of Lucknow District, North India. Transactions of Royal Society of Tropical Med and Hygiene.2008. (in press). 86. Prasad A, Prasad KN, Yadav A, Gupta RK, Pradhan S, Jha S, Tripathi M and Husain M. Lymphocyte Transformation Test: A new method for diagnosis of Neurocysticercosis. Diagnostic Microbiology and Infectious Diseases.2008. (in press). 87. Trivedi R, Rathore RKS, Gupta RK. Clinical applications of diffusion tensor imaging - A pictorial assay. Indian J Radiol and Imaging 2008 (in press). 76.

20

CURRICULUM VITAE
Name: Address:

DR. MAZHAR HUSAIN

2/182. Vishwas Khand, Gomtinagar, Lucknow-226010, India Phone: Office: 91-522-2257450, ext.238 Resi: 91-522-2307171 / 2308077 Fax: 91-522-2257606 Mobile: 91-9415021729 E-mail: mazharhusain@hotmail.com mazharhusainlucknow@yahoo.com Date of Birth: August 8th, 1952

Present Position:
Head of Department & Professor of Neurosurgery, King George's Medical University, Lucknow-226003, India. Chief Consultant Neurosurgeon, GM & Associated Hospitals, Lucknow, India.

Qualifications:
B.Sc. (Hons) 1970, A.M.U., Aligarh, India. M.B,B.S. 1975, King Georges Medical College. University of Lucknow, India. M.S. (General Surgery) 1979. King George's Medical College, University of Lucknow, India. MCh. (Neurosurgery) 1983. King George's Medical College. University of Lucknow, India.

Professional experience:
Junior, Senior &Chief Resident : Dept of Gen Surgery, King George Medical College, Lucknow, India 21 Jan 77 to 17 July 79 Chief Resident in Neurosurgery King George Medical College, Lucknow, India. 18 July 80 to 27 June 83 Senior Resident in Neurosurgery Dr. R.M.L. Hospital. New Delhi, India. 28 Dec 83 to 26 Feb 84 Lecturer in Neurosurgery J.N. Medical College. Aligarh, India. 27 Feb 84 to 20 June 84 Lecturer in Neurosurgery King George Medical College. Lucknow, India. 21 June 84 to 31 Dec 85 Assistant Prof of Neurosurgery King George Medical College. Lucknow, India. 01 Jan 86 to 17 July 89 Associate Prof of Neurosurgery King George Medical College. Lucknow, India. 18 July 89 to 16 Apr 97 Professor, Department of Neurosurgery- King George Medical College. Lucknow, India 16 April 97 till date Head, Department of Neurosurgery King George Medical College. Lucknow, India. l8 Oct 94 till date

Licentiate Exam:
Passed ECFMG examination of July 21, 1976, Certificate No. - 256-601-C dated April 21st 1977.

Research Publications (2004 onwards):

21

1. 2. 3.

4. 5.

6. 7.

8.

9. 10.

11.

12. 13. 14.

Husain M, Jha D, Thaman D, Husain N, Gupta RK.--Ventriculostomy in a tumor involving the third ventricular floor. Neurosurgical Review; 2004; 27:70-72 Nirala AP, Husain M, Vatsal DK, A retrospective study of multiple interbody grafting and long segment strut grafting following multilevel anterior cervical decompression. British Journal of Neurosurgery, 2004 Jun; 18(3):227-32. Gupta C, Husain M, Kumar M, Kohli N, Tiwari V, Vatsal DK, Nirala AP, Transcranial Doppler sonography evaluation in patients with vasospasm following subarachnoid haemorrhage.Journal of the Indian Medical Association;2004; 102(4): 191-196. Garg. M, Gupta RK, Husain M, Chawla S, Chawla J, Kumar R, Rao SB,Misra MK, Prasad KN, Brain Abscess:Etiologic Characterization with in Vivo Proton MR Spectroscopy. Radiology 2004; 230:519-527 CRASH trial collaborators (Husain M -NC-India). Effect of intravenous corticosteroids on death within 14 days in 10008 adults with clinically significant head injury (MRC CRASH trial): randomised placebo-controlled trial. Lancet 2004; 364: 13211328. Agarwal A, Raghav S, Husain M, Kumar R, Gupta R,-- Epilepsy with focal cerebral calcification: Role of magnetization transfer MR imaging. Neurology India 2004 : 52-2: 197-199 Agarwal M, Chawla S, Husain N, Jaggi RS, Husain M, Gupta RK. Higher succinate than acetate levels differentiate cerebral degenerating cysticerci from anaerobic abscesses on in-vivo proton MR spectroscopy. Neuroradiology. 2004 Mar;46(3):211-5. Mishra AM, Gupta RK, Jaggi RS, Reddy JS, Jha DK, Husain N, Prasad KN, Behari S, Husain M. Role of diffusion-weighted imaging and in vivo proton magnetic resonance spectroscopy in the differential diagnosis of ring-enhancing intracranial cystic mass lesions. J Comput Assist Tomogr. 2004 JulAug;28(4):540-7. Husain M, Jha D, Agarwal S, Husain N, Gupta RK, Conical Working Tube: A special device for endoscopic surgery of lumbar disc herniation. Journal of Neurosurgery-Spine, 2005; 3:265-270 Gupta RK, Agarwal A, Agarwal S, Mohan P, Husain M, Alger JR, Pandey CM, Datta D. Reversal of Acute Human Brain Ischemic Injury By Lysine Induced Therapeutic Angiogenesis: Preliminary Results Of A Pilot Study. The Internet Journal of Neurology. 2005. Volume 4 Number 1. Edwards P, Arango M, Balica L, Cottingham R, El-Sayed H, Farrell B, Fernandes J, Gogichaisvili T, Golden N, Hartzenberg B, Husain M. Final results of MRC CRASH, a randomised placebo-controlled trial of intravenous corticosteroid in adults with head injury-outcomes at 6 months. Lancet. 2005; 365:1957-1959. Gupta RK, Hasan KM, Mishra AM, Jha D, Husain M, Prasad KN, Narayana PA. High fractional anisotropy in brain abscesses versus other cystic intracranial lesions. AJNR Am J Neuroradiol. 2005 May; 26(5):1107-14. Husain M, Rastogi M, Jha DK. Endoscopic Third Ventriculostomy through the Interfornicial Space Pediatric Neurosurgery 2005;41:165-167 Husain M, Jha DK, Rastogi M, Husain N,Gupta RK. Role of neuroendoscopy in the management of patients with tuberculous meningitis hydrocephalus. Neurosurg Review. 2005, 28(4):278-283.

22 15. Gupta RK, Saksena S, Agarwal A, Hasan KM,Husain M, Gupta V, Narayana PA. Diffusion tensor imaging in late posttraumatic epilepsy. Epilepsia. 2005; 46(9):1465-71. 16. Gupta RK, Prakash M, Mishra AM, Husain M, Prasad KN, Husain N. Role of diffusion weighted imaging in differentiation of intracranial tuberculoma and tuberculous abscess from cysticercus granulomas-a report of more than 100 lesions. Eur J Radiol. 2005; 55(3):384-92. 17. Mishra AM, Gupta RK, Saksena S, Prasad KN, Pandey CM, Rathore RK, Husain N, Jha DK, Jaggi RS, Husain M. Biological correlates of diffusivity in brain abscess. Magn Reson Med. 2005; 54(4):878-85. 18. Husain M, Jha DK, Rastogi M. Angiographic catheter: unique tool for neuroendoscopic surgery. Surgical Neurology. 2005; 64(6):546-9. 19. Jaggi RS, Husain M, Chawla S, Gupta A, Gupta RK. Diagnosis of bacterial cerebellitis: diffusion imaging and proton magnetic resonance spectroscopy. Pediatr Neurol. 2005 Jan; 32(1):72-4. 20. Prasad KN, Mishra AM, Gupta D, Husain N, Husain M, Gupta RK. Analysis of microbial etiology and mortality in patients with brain abscess. J Infect. 2006 Oct; 53(4):221-7 21. Husain M, Rastogi M, Ojha BK, Chandra A, Jha D, Endoscopic transoral surgery for craniovertebral junction anomalies. Journal of Neurosurgery-Spine, 5:367-373, 2006 22. Mishra AM, Reddy SJ, Husain M, Behari S, Husain N, Prasad KN, Kumar S, Gupta RK. Correlation of magnetization transfer ratio and fluid-attenuated inversion recovery imaging signal intensity with biological parameters in cystic intracranial mass lesions. J Magn Reson Imaging 2006 Jun 9; 24(1):52-56. 23. Reddy JS, Mishra AM,Behari S, , Husain M, Gupta V, Rastogi M, Gupta RK. The role of diffusion-weighted imaging in the differential diagnosis of intracranial cystic mass lesions: a report of 147 lesions. Surgical Neurology. 2006 Sep; 66(3):246-50. 24. Gupta RK, Haris M, Husain N, Husain M, Prasad KN, Pauliah M, Srivastava C, Tripathi M, Rastogi M, Behari S, Rathore D, Rathore RKS. Relative cerebral blood volume is a measure of angiogenesis in brain tuberculoma and its therapeutic implications. J Comput Assist Tomogr. 2007 May/June;31(3):335341. 25. Haris M, Gupta RK, Husain N, Hasan KM, Husain M, Narayana PA, Measurement of DTI metrics in hemorrhagic brain lesions: possible implication in MRI interpretation. J Magn Reson Imaging. 2006 Dec;24(6):1259-68. 26. 78. Ojha B.K. Sharma, M.C. Rastogi, M. Chandra, A. Husain, M. Husain, N., Dumbbell-Shaped Paraganglioma of the Cervical Spine in a Child. Pediatric Neurosurgery 2007;43:60-64. 27. Husain M, Jha D, Rastogi M, Husain N, Gupta RK, Neuro-endoscopic management of intraventricular neurocysticercosis (NCC). Acta Neurochirurgica (Wien). 2007 Apr;149(4):341-346. 28. Husain M, Rastogi M, Jha DK, Husain N, Gupta RK. Endoscopic transaqueductal removal of fourth ventricular neurocysticercosis using angiographic catheter. Neurosurgery 2007 Apr;60(4 Suppl 2):249-53. 29. Gupta RK, Haris M, Husain N, Husain M, Prasad KN, Pauliah M, Srivastava C, Tripathi M, Rastogi M, Behari S, Singh A, Rathore D, Rathore RK. Relative cerebral blood volume is a measure of angiogenesis in brain tuberculoma. J Comput Assist Tomogr. 2007 May-Jun;31(3):335-41.

23 30. Luthra G, Parihar A, Nath K, Jaiswal S, Prasad KN, Husain N, Husain M, Singh S, Behari S, Gupta RK. Comparative evaluation of fungal, tubercular, and pyogenic brain abscesses with conventional and diffusion MR imaging and proton MR spectroscopy. AJNR Am J Neuroradiol. 2007 Aug;28(7):1332-8. 31. Husain N, Husain M, Rao P. Pituitary tuberculosis mimicking idiopathic granulomatous hypophysitis.Pituitary. 2007 Sep 5 32. Singh I, Haris M, Husain M, Husain N, Rastogi M, Gupta RK. Role of Endoscopic Third Ventriculostomy in Patients with communicating Hydrocephalus-An Evaluation by MR Ventriculography. Neurosurg Review (in press). 33. Nath Kavindra, Husain Mazhar, Trivedi Richa, Kumar Raj, Prasad Kashi N, Rathore RKS, Gupta RK. Clinical Implications of Increased Fractional Anisotropy in Meningitis associated with Brain Abscess. JCAT (in press). 34. Gupta RK, Nath Kavindra, Prasad Amit, Prasad Kashi N, Husain Mazhar, Rathore Ram K.S, Husain Nuzhat, Srivastava Chhitiz, Khetan Prakash, Trivedi Richa, Narayana PA. In vivo Demonstration of Neuroinflammatory Molecules Expression in Brain Abscess with Diffusion Tensor Imaging. AJNR (in press). 35. Husain N, Shukla N, Kumar R, Husain M, Chaturvedi Arun, Agarwal Girdhar G, Gupta Rakesh K. ELISA in the evaluation of therapeutic response to albendazole in neurocysticercosis. Journal of Infection (In-press)

Research Projects:
Current Projects: Principal investigator: MR correlates of post traumatic epilepsy, ICMR, New Delhi. Co-investigator: 1) ELISA in the Assessment of Therapeutic Response in Neurocysticercosis. IndiaCLENUSAID project. 2) Fingerprinting of causative bacteria in bacteria in brain abscess using different Magnetic Resonance techniques in humans and animal models. ICMR, New Delhi. Completed Projects: (1995-2004) Co Investigator: 1. Immunohistochemical expression of P53 in Human CNS Malignancies. (Department of Science &Technology, New Delhi) 2. An evaluation of ELISA in the diagnosis and follow-up of Neurocysticercosis. (UP Council of Science Technology, Lucknow) 3. Immunoblot Analysis of Cysticercus fasciolaris antigen in the diagnosis of Neurocysticercosis. (Indian Council of Medical Research, New Delhi , 1998-2000) 4. National: Coordinator: CRASH-Trial Corticosteroid randomization after significant head injury Multicentric International placebo controlled double blind clinical trial to asses the role of high dose methylprednisolone in the outcome of head injury patients. Conducted by London School of Hygiene and Tropical Medicine, funded by Medical Research Council UK.