DOC # 100356

REV D

ANAEROBE SYSTEMS

Product Insert
TRYPTIC SOY-SERUM BACITRACIN VANCOMYCIN AGAR (TSBV)
Products:
AS-648 Tryptic Soy-Serum Bacitracin Vancomycin Agar (TSBV) 4 plates / pkg

Intended Use
TSBV is an enriched selective medium for the isolation and presumptive identification of Actinobacillus actinomycetemcomitans.

Summary
TSBV medium contains bacitracin and vancomycin at a concentration that inhibits most gram-positive and most gram-negative
anaerobes, except for Actinobacillus actinomycetemcomitans among others. This medium is prepared, dispensed and packaged
under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Formula
Tryptic Soy Agar 40.0 g
Yeast Extract 1.0 g
Bacitracin 75.0 mg
Vancomycin 5.0 mg
Horse Serum 100.0 ml
Distilled Water 1000.0 ml
Final pH 7.0 +/- 0.2 at 25 degrees C.

Precautions
For IN VITRO DIAGNOSTIC USE only. Approved biohazard precautions and aseptic techniques should be observed when using
this product. This product is for use only by properly-trained and qualified personnel. Sterilize all biohazard waste prior to
disposal.

Storage and Shelf Life

Storage: Upon receipt, store at room temperature in original container until use. Avoid overheating or freezing. Do not use
medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination.
The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration
date shown on the container.
Shelf Life: 90 days from date of manufacture.

Procedure
Specimen Collection: Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and
processing. Consult appropriate references for detailed instructions concerning collection and transport of anaerobes.
Methods for Use: TSBV should be inoculated directly with clinical material or a broth that has been previously inoculated from
clinical material. Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an anaerobic
atmosphere and incubated at 35-37oC for 18 – 48 hours. Additional periods of incubation may be necessary to recover some
anaerobes. Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

Page 1 of 2 Anaerobe Systems

15906 Concord Circle Morgan Hill, CA 95037 408 782 7557 Fax 408 782 3031
http://www.anaerobesystems.com
 DOC # 100356
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Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator /
autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

Limitations
TSBV will not provide complete information for identification of bacterial isolates. Additional test procedures and media are
required for complete identification. It is recommended that a non-selective medium such as Anaerobic Brucella Blood Agar
also be inoculated from clinical specimens to assure growth of all species present. Consult reference materials for additional
information.

Quality Control
This medium should support good growth of A. actinomycetemcomitans found in clinical infections.
The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.
Organism Tested ATCC # Results Time
(Hours)
Actinobacillus actinomycetemcomitans 29522 Growth 24 – 48 h
Actinomyces viscosus 43146 No Growth
Fusobacterium nucleatum 25586 Growth 24 – 48 h
Propionibacterum acnes 9619 No Growth
Clostridium difficile 9689 No Growth
Enterococcus faecalis 29212 No Growth

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by
the end user.
If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be
incubated anaerobically and aerobically for 48 – 96 hours.
If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC
organisms be evaluated for growth/inhibition.
Organism Expected Growth
A. actinomycetemcomitans 24-48 hrs
A. viscosus Inhibited
F. nucleatum 24-48 hrs
E. faecalis Inhibited

Physical Appearance: TSBV should appear translucent yellow in color.

References
1. Dowell, V. R., Jr. and T. M. Hawkins. 1974. Laboratory Methods in Anaerobic Bacteriology. CDC Laboratory
Manual. USDHEW C. D. C. Atlanta, GA 30333.
2. Engelkirk, P. G., Duben-Engelkirk, J., Dowell, V. R. 1992. Principles of Practices of Clinical Anaerobic
Bacteriology. Star Publishing Co., Belmont, CA 94002.
3. Holdeman, L. V., F. P. Cato and W. E. C. Moore. 1977. Anaerobe Laboratory Manual. Virginia Polytechnic Institute
and State University. Blacksburg, VA 24061.
4. Slots, J. 1982. Selective Medium for Isolation of Actinobacillus actinomycetemcomitans. Journal of Clinical
Microbiology. April 1982, p. 606 – 609.
5. Somer-Jousimies, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. and S. M. Finegold. 2002.
Wadsworth – KTL Anaerobic Bacteriology Manual. Star Publishing Co., Belmont, CA 94002
6. NCCLS. Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third
Edition. (2004). NCCLS document M22-A3. NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.

Revision Date: 10/16/04

Page 2 of 2 Anaerobe Systems

15906 Concord Circle Morgan Hill, CA 95037 408 782 7557 Fax 408 782 3031
http://www.anaerobesystems.com