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GENE-TRAK®
Listeria DLP Assay

Results

Positive Negative Positive Negative

Control Control Sample Sample

Product Specifications
Sensitivity: 1-5 CFU/25g
Testing time: 2 hours (after 40-48 hour enrichment)
Tests per kit: Up to 98
Approvals: AOAC-RI #981201
Intended Use
GENE-TRAK Listeria Direct Labeled Probe (DLP) Assay provides for Materials Required But Not Provided
the detection of Listeria spp. in food and environmental samples. Available from Neogen*
1. GENE-TRAK photometer set
The Test 2. Test tube racks
The DNA hybridization test employs Listeria-specific DNA probes 3. Dipstick holders
directly labeled with horseradish peroxidase and a colorimetric detec- 4. Wash basins with lids
tion system for the detection of Listeria in food samples following broth 5. Dilution chart
culture enrichment. A sample is considered negative for the presence of 6. Pipettes (1 mL and 10 mL)
Listeria if the absorbance value obtained is less than or equal to the estab- 7. Incubator at 35°C
lished cutoff value for the assay. A sample is considered presumptively 8. 12 x 75 mm glass test tubes
positive for the presence of Listeria if the absorbance value obtained is 9. Minute timer
greater than the established cutoff value of the assay. 10. Micropipette and tips, 100 – 1000 µL range (optional)
11. Modified LCA
Test Procedure
1. Enrich food samples. Not available from Neogen
2. Add 0.2 mL of enriched samples, negative control and positive 1. Blender or homogenizer
control to separately labeled 12 X 75 mm glass tubes. 2. Culture bottles for sample enrichments
3. Add 0.1 mL pretreatment reagent and 0.1 mL of lysis reagent 3. Phosphate-buffered Listeria enrichment broth
to each sample tube. Shake for 5 seconds and incubate at 37°C 4. Phosphate-buffered saline
for 15 minutes. 5. UVM-1 broth
4. Add 0.1 mL of denaturing solution and incubate the tubes at 6. UVM-2 broth
37°C for 5 minutes. 7. Modified UVM-2 broth
5. Add 0.1 mL of neutralizing solution, 0.4 mL hybridization solu- 8. Sterile cotton swabs
tion and 0.1 mL Listeria probe solution to each tube. 9. Water bath or heater block capable of maintaining 37°C
6. Place dipsticks into sample tubes. Raise and lower dipsticks 10 10. Graduated cylinder
times to mix, and incubate at 37°C for 1 hour. 11. Absorbent paper
7. Wash dipsticks two times for 1 minute each at room tempera- 12. Sterile tubes with caps for 1 mL volume
ture. * See Equipment and Accessories, pages 91–95
8. Transfer dipsticks to tubes containing 0.75 mL of substrate-
chromogen and incubate at room temperature for 30 minutes.
9. Remove dipsticks and add 0.25 mL stop solution to each tube.
10. Read results using a GENE-TRAK photometer.
See package insert for complete instructions
Ordering Information
Prod.# Product description
6706 GENE-TRAK Listeria DLP Assay - up to 98 tests

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