SANJA GLISIC, PH.D.

West Allis, WI 53227 414 545 0681 (evening hours) sglisic@hotmail.com http://www.linkedin.com/in/sanjaglisic

SR.SCIENTIST MOLECULAR BIOLOGY/GENETICS

Objective: Accomplished researcher with extensive experience in fields of molecular genetics, immunogenetics, functional genomics and immunology, seeking a senior scientist position. Core expertise: More than 20 years of laboratory experience Experienced in molecular genetics techniques, like PCR and variants, genotyping, cloning, sequencing, DNA, RNA, RT-PCR, Western blot, Northern blot, Fluorescent Activated Cell Sorter (FACS) technology, cell culture techniques, radioactive and non-radioactive stainings, immunoassays Knowledgeable of strategic thinking, research planning and budget planning and executions, analyzing results, writing reports and publications, and authoring research proposals based on generated results. Thrives in independent and collaborative lab settings and works well with staff at all levels and across disciplines. Displays comprehensive knowledge of interplay of cell types, genes and environmental factors and impact on development of complex diseases, specifically Type 1 Diabetes and atherosderosis. Capable of managing technicians and research associates; mentored students. Published in several leading scientific journals; repeatedly recognized for contributions to current research activities. Valued and respected professional who prioritizes tasks effectively and adapts easily to challenging projects, priorities, and research focus.

SUMMARY OF ACCOMPLISHMENTS 38 peer-reviewed publications Developed dual fluorescent-based method for apoptosis with broad application using FACS technology and Tregspecific fluorescent-based apoptosis method with increased Treg specificity Developed genotyping PCR strategy for HCV, CMV and B.burgdorferii detection in patient samples (blood, urin, liquor, tissues) PROFESSIONAL EXPERIENCE
MEDICAL COLLEGE OF WISCONSIN, Milwaukee, WI 2003-Present DEPT. OF PEDIATRICS - MAX MCGEE NATIONAL RESEARCH CENTER FOR JUVENILE DIABETES Assistant Professor, 2008-Present Investigate apoptosis of CD4+CD25+high T-cells (regulatory T-cells or TREGS, cell subset crucial in immune homeostasis) and association of apoptosis and genetic (MHC) background in Type I diabetes (T1D). Characterize TREG cells and research mechanisms of spontaneous apoptosis of TREGS in human T1D, using gene expression microarrays. Create inducible regulatory T-cells (iTREGS) from effector T-cells, characterize by comparing gene expression and functional behavior to naturally occurring and expanded TREGS. Assess reported decreases in regulatory function of TREGS in pediatric subjects with T1D. Apply TREGS apoptosis as risk assessment tool; evaluate role of TREGS apoptosis in autoimmune diseases. Develop and utilize specific apoptosis stain combination to detect that increased TREG apoptosis decreased function in recent-onset T1D subjects and in multiple autoantibody-positive unaffected subjects. Research Scientist, 2005-2008 Conducted research on apoptosis of CD4+CD25+high T-cells (regulatory T-cells) and pathways in diabetes. Produced regulatory T-cells for T1D therapeutic purposes and worked with recent-onset T1D subjects as most informative group of subjects for understanding disease pathogenesis. Developed precise criteria for existence and cessation of honeymoon period.

Postdoctoral Fellow, 2003-2005 Researched differences in CD4+CD25+ T-cell population properties between diabetic patients and controls. Investigated apoptosis methods suitable for human PBMCs; determined apoptosis signaling pathways increased in diabetes versus control subjects. UNIVERSITY OF NOTRE DAME, Notre Dame, IN 2000-2003 W.M.KECK CENTER FOR TRANSGENE RESEARCH Postdoctoral Research Fellow Developed and executed investigations targeting vector generation for TAFI and EPCR genes and plasminogen mutated forms generation at DNA level and protein production of generated mutated forms. INSTITUTE OF NUCLEAR SCIENCES, Vinca, Belgrade, Yugoslavia 1988-2000 INSTITUTE FOR RADIOBIOLOGY AND MOLECULAR GENETICS Principle Scientist, 1998-2000 Developed novel method for Hepatitis C Virus (HCV) genotyping. Conducted genetic studies of association of gene polymorphisms (ApoE, ACE I, apo(a)) in healthy Serbian population and in patients. Research Associate, 1992-1998 Investigated association of ApoB gene polymorphisms in normolipidemic Serbian population and serum lipid levels. Research Assistant, 1988-1992 Supported research investigations focused on screening of ApoB 3end polymorphisms gene in normolipidemic Serbian population and association with total and LDL cholesterol.

EDUCATION
UNIVERSITY OF BELGRADE - FACULTY OF NATURAL SCIENCES AND MATHEMATICS, Yugoslavia 1998 Ph.D. in Molecular Biology and Physiology-Genetics, 1998 Ph.D. equivalent to U.S. - evaluation performed by Globe Language Services, New York, NY, 2003; evaluation performed by World Education Services (WES), New York, NY 2010. Master of Science in Molecular Biology and Physiology, 1992 Awarded Graduate Fellowship, Government Department for Science and Technology, 1989-1991 Awarded Travel Award from EEMS Conference, Toulouse, France, 1997 Bachelor of Science in General Biology, 1988

PROFESSIONAL AFFILIATIONS
American Diabetes Association Serbian Genetics Society European Environmental Mutagenesis Society

JOURNAL AND GRANT REVIEWER

Ad hoc NIH reviewer: NIH Special Emphasis Panel/Scientific Review Group 2009/01 ZRG1 IMM-G (02) M, 2009 and 2010/01 ZRG1 IMM-G (02) M, 2010 Reviewer for Advances in Medical Sciences, 2008-present Reviewer for Clinical and Experimental Immunology, 2008-present Reviewer for PLoS ONE, 2010-present

LANGUAGES
Fluent in English and Serbian, reads and writes in German

Complete list of grant support, publications, and abstracts available upon request.