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GB15979-1995
2002-03-05
2002-09-01
GB159791995 1996
GB159791995
A G
GB 159792002
GB 15981-1995
4
4.1
4.2
4.3 1
GB 159792002
1
1)
cfu/g
cfu/g
2)
cfu/mL
cfu/g cfu/mL
200
100
200
100
20
200
100
10 000
20
200
100
10 000
20
200
100
10 000
20
20
1)
2)
4.4 1 90
90 1
4.5 1
5026
5026 1
4.5 250g/g
5
5.1 2 500 cfu/m3
2
GB 159792002
5.2 20 cfu/cm2
5.3 300 cfu/
6
6.1 (ATCC 9372)103
6.2 E6d(ATCC 27142)103
6.3 (ATCC 7953)103
7
7.1
7.1.1 3.1
7.1.2 A
7.1.3 B
7.1.4 C
7.1.5 D
7.2 E
7.3 F
8
8.1
8.2
8.3
9
9.1
9.2
9.3
5
9.4
9.5
9.6
3
GB 159792002
9.7
9.8
9.9 ()(
)
10
10.1
10.2
10.3
10.4
11
11.1
11.2
12
12.1
12.2
GB 159792002
A1
A1
A1
1
A2
1999
A3
1
2
A3
A3.1
A3.2
5
GB 159792002
A3.2.1
1g/10mL
371 24h
A3.2.2
A4
1999
GB 159792002
B1
12 14 14
12
100 3 10 g1g
200 mL
50mL
B2
B2.1
5 1 mL
45 15 20mL
352 48h
B2.2
(B1)
X1=A
K
(B1)
5
X1cfu/g cfu/mL
A5
K
100 100
B2.3
B2.3
2 2
1
B3
7
GB 159792002
B3.1
5mL 50mL 352 24 h
352 1824 h
12 352 24 h
B3.2
B4
B4.1
5 mL 50 mL SCDLP 352 1824 h
352 1824 h
130 s
23 352 24 h
35 mL
1 mol/L 1 mL
352 24 h
352 24h 4
10
42 42 2448 h
GB 159792002
B4.2
42
B5
B5.1
5 mL 50 mL SCDLP 352 24 h
12 352 2448 h
352 24 h
5 min
14 0.5 mL 24 h 0.5 mL 35
224 h
0.5 mL
B5.2
B6
B6.1
5 mL 50 mL 352 24 h
352 24 h
0.2 mL(0.01 g 5 mL )
0.8 mL 24 h 0.5 mL 0.25 0.25 mL
3522 min ( 10 min )
9
GB 159792002
2 h 24 h 24 h
0.04
352 1824 h
B6.2
B7
B7.1
5
1mL
45 1525 mL
252 7 357
B7.2
B2
X2=A
K
(B2)
5
X2cfu/g cfu/mL
B5
K
100 100
B7.3
B7.3
2 2
1
B8
B8.1
5mL 50mL 252 7
B8.2
10
GB 159792002
C1
20
5 5 10
C2
C2.1
C2.1.1 (ATCC 6538) (8099 ATCC 25922)
C2.1.2 ATCC 10231
314 (1824h) 5mL0.03mol/L
PBS PBS
C3
C3.1
C3.1.1
1)5mLPBS
2)5mL
3)5mL
4)5mL
5)5mLPBS
6) PBS
7)
8)
C3.1.2
1) 1
2) 2 1 345
3) 345 11049104cfu/
15
4) 68
11
GB 159792002
5) 3
C3.2
C3.2.1
24h PBS 100L
11049104cfu/
2.0cm3.0cm
4 4 4
100L 25
1020min 5mL
23 0.5mL 4045
15mL 352 48h
72h
3 C1
X3(A-B)/A100%
C1
X3
A
B
C3.2.2
90
C4
C4.1
24h PBS 100L 5mL
11049104cfu/ mL
2.0cm3.0cm5mL
4 4
100L
251020min0.5mL 5mLPBS
23 0.5mL 4045
15mL
352 48h 72h
3 C2
12
GB 159792002
X4(A-B)/A100%
C1
X4
A
B
C4.2
509090
C5
C5.1
1.0cm1.0cm 0.75g
0.75g 250mL 70mLPBS 5mL PBS
11049104cfu/mL
300r/min 1h
0.5mL PBS
3 C3
X5(A-B)/A100%.
C1
X5
A
B
C5.2
11049104cfu/mL 10
26
C6
C6.1
C6.1.1 1
C6.1.2 5457 14 3740 3
75
C6.2
13
GB 159792002
C3 C4 C5
54 C3 C4 C5
37 C3 C4 C5
14
GB 159792002
D
D1
D2
()
24 h 4.6
D3
(FID)
Chromosorb 101 HP6080 2m 3 mm120
150
150
35 mL/min
35 mL/min
350 mL/min
108 kPa
D4
D4.1
100 mL ()
10 mL 100 mL 10 mL 100 mL(
10 mL 90 mL )
23 ( 1 00010 000 )
C=
273
44 10 6
(D1)
3
22.4 10 K 273 + t
c g/mL
k
15
GB 159792002
t
D4.2
2 2 g 5 mL
4h 30 min
2mL
D4.3
1.0 mL 2
L 2
()
D4.4
(g)()
() A(g)(D2)
X =
A
M
V( )
V()
(D2)
Xg/g
Ag
Mg
V()mL
V()mL
16
GB 159792002
E1
E1.1
30 m2 1 m 30 m2
5 4 1 m
( 9 cm)()
5 min
E1.2
352 24 h
6 h 352 48h
E1.3
Y1 =
A 50000
(E1)
S1 t
y1cfu/m3;
A
S1cm2;
tmin
E2
E2.1
5cm5cm
10 10mL
10
10mL
E2.2
6h 1mL
352 48 h
17
GB 159792002
Y2 =
Y3 =
A
10
S2
(E2)
A
10
S3
.(E3)
y2cfu/cm2;
A
S2cm2;
y3cfu/
E2.3
B
18
GB 159792002
F
F1
F1.1 (ATCC 9372) 51055
106cfu/ 600mg/L30mg/L 542 6010
90 D 2.55.8 min7.5 min58 min
F1.2 10
352
24 h 7
103
F2
F2.1 E601(ATCC 27142) 51055106
cfu/ 90 D10 1.7kGy
F2.2 5 3
352 24 h 7
103
F3
GB159811995
19
GB 159792002
G1
10g
3g
5g
15g20g
1 000mL
pH 7.27.4121
15min
G2
20 g
()
5g
10 g
0.04
25 mL
1 000 mL
pH 7.4 50 mL
115 15 min
G3
20 g
10 g
0.04
25 mL
1 000 mL
pH 7.4 10 mL
115 15 min
20
GB 159792002
G4 (EMB)
10 g
10 g
2g
17 g
2 Y
20 mL
0.65
10 mL
1 000 mL
pH 7.1121 15 min
55
G5 SCDLP
17 g
3g
5g
2.5 g
2.5 g
1g
80
7g
1 000 mL
() pH 7.27.3
121 20min 80 25
G6
3g
10 g
5g
0.3 g
20 g
1 000 mL
21
GB 159792002
pH 7.47.6115 20 min
55
G7
20 g
1.4 g
10 g
18 g
()
10g
1 000 mL
pH 7.4
115 20 min
G8
3g
5g
120 g
1 000 mL
10 g
3g
2g
0.5 g
1 000 mL
pH 7.2
115 20 min
G10
22
GB 159792002
100 mL
()
10 mL
55 10 mL
G11
10 g
5g
5g
10 g
0.2
12 mL
1000 mL
pH 7.4
115 20 min
G12
10g
5g
5g
10g
1 000mL
pH 7.27.4121 15min
G13
3.8 1 4 3 000 r/min 5 min
G14
10 g
40 g
20 g
1 000 mL
700 mL 300 mL
115 15 min 0.1g/L 0.03g/L
23
GB 159792002
G15
10 g
5g
3g
1 000 mL
pH 7.27.4115 30 min
G16
10 g
5g
1 000 mL
1g
95
20 mL
80 mL
1g
2g
300 mL
95
(1)
0.25 g
95
10 mL
90 mL
(2)
24
GB 159792002
10g 95 100mL 10mL 5
90mL 10mL 90mL
G18 0.03 mol/L PBSpH7.2
2.83 g
1.36 g
1 000 mL
25