Ultra Fast Speed

Fusion of Ultra Fast MRM and Ultra Fast Polarity Switching An Ultra Fast Scan Speed of 15,000 u/sec 500 MRMs per Second, the Most Ever Possible

High Reliability
Robust System Provides Long-term Data Stability UFsweeperTM Technology Dramatically Minimizes Cross Talk Excellent Linearity with Wide Dynamic Range

User-Friendly
Easy Maintenance for Minimized Instrumetn Downtime Single-vendor Solution Provides Seamless Operation Quantitation Browser for Effective Multi-analyte Quantitation

CE - 20V - 40V

26 pharmaceutical compounds were analyzed using Shimadzus Synchronized Survey ScanTM (SSS) shown in Figure 1. In this mode, full scan measurement are rapidly followed by automated product ion scanning. High-speed polarity switching (15 msec) and rapid scan rates (15000u/sec) allow multiple collision energies to be employed for unknowns even with narrow peak widths. This enables molecular weight confirmation from the Q3 scan data and also generates structural fragmentation information from the same peak. A total of 26 pharmaceutical compounds were evaluated. Figure 2 shows the analytical result for Cilostazol and Warfarin. As illustrated in Table 1, all compounds were detected in either positive mode, negative mode, or both, demonstrating the LCMS-8030s effectiveness for drug discovery and synthesis confirmation.
40000000 40000000 30000000 20000000 10000000 0 0.0 Inten. (x10,000,000) 1.0 2.0 min 1.0 10000000 1:TIC(+) 2:TIC(+) 3:TIC(+) 4:TIC(-) 5:TIC(-) 6:TIC(-) 1:TIC(+) 2:TIC(+) 3:TIC(+) 4:TIC(-) 5:TIC(-) 6:TIC(-)

20V 40V

Fig. 1 MS method using SSS function Table 1 Detection of 26 compounds

Compound 1 Atenolol 2 Procaine 3 Lidocaine 4 Atropine 5 Yohimbine 6 Chlorpheniramine 7 Propranolol MW 266.16 236.15 234.17 289.17 354.19 274.12 259.16 249.17 264.18 255.16 279.16 266.18 280.19 263.17 277.18 454.28 236.09 230.14 308.08 342.04 369.22 346.12 284.07 308.10 322.01 360.13 Detection Positive Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No No Negative No No No No Yes No No No No No No No No No No No No No No No Yes Yes No Yes Yes Yes

Cilostazol

30000000

Warfarin

20000000

0 0.0 Inten. (x10,000,000) 1.0 2.0 min

8 Alprenolol 9 Tetracaine 10 Diphenhydramine

370.2
1.0

307.2

Q3 Scan(+)
C20H27N5O2 MW :369.46

0.5

Q3 Scan(-)
C19H16O4 MW :308.33

11 Doxepin 12 Desipramine 13 Imipramine

0.0 200 Inten. (x1,000,000) 300 400 m/z

0.0 200 Inten. (x100,000) 7.5 300 400 m/z

14 Nortriptyline 15 Amitriptyline 16 Verapamil 17 Carbamazepine 18 Isopropylantipyrine 19 Alprazolam

2.0

125.1

Product ion scan CE -20V

288.1 176.1 370.2

5.0

Product ion scan CE 20V

161.1 250.0 57.3

307.1

1.0

55.2

2.5

0.0 100 Inten. (x1,000,000) 200 300 m/z

0.0 100 Inten. (x100,000) 200 300 m/z

20 Triazolam 21 Cilostazol 22 Nifedipine 23 Diazepam 24 Warfarin 25 Chloramphenicol

55.1
1.0

Product ion scan CE -40V

125.1
1.0

57.4 117.0

Product ion scan CE 40V

161.1 206.1 250.1
200 300 m/z

0.5

83.1
0.0 100

146.0
0.0 200 300 m/z 100

Fig. 2 Analytical results (Cilostazol, Warfarin) Table 2 Analytical Conditions : Shim-pack XR-ODSII (2.0 mmI.D. 50 mmL.) : 5 mmol/L ammonium formate-water : acetonitrile : 5 %B (0 min) 95 %B (3-3.50 min) 5 %B (3.51-5 min) Flow rate : 0.3 mL/min Injection volume : 5 uL CDL temperature : 250 C Block heater temperature : 400 C Column Mobile phase A Mobile phase B Gradient program

26 Nitrendipine

Nebulizing gas flow Drying gas flow Column temperature Collision energy Q1/Q3 resolution Scan type Scan range Ionization mode Scan speed

: 1.5 L/min : 10 L/min : 40 C : pos 20V, 40V neg 20V, 40V : Unit/Unit : Q3 scan - product ion scan : m/z 200-500 : ESI positive and ESI negative : 3333 u/sec