LabChip | EZ Reader Series

Benchtop Readers for Enzymatic Assays and Selectivity Profiling

The LabChip EZ Reader Series is Calipers next generation microfluidic instrumentation for drug screening. Using cutting edge microfluidic technology, the LabChip EZ Readers enable you to perform a broad range of enzymatic assays on a single integratable platform. The precision of microfluidics allows researchers to detect both strong and weak inhibitors with high accuracy, and routinely identify drug candidates that alternative technologies miss.

LabChip EZ Reader Series

Mobility Shift Assay Technology- The Caliper Advantage...

Utilizing Calipers widely adopted mobility shift microfluidic assay technology, the LabChip EZ Reader II is a bench top instrument that analyzes enzyme activity by sipping reactions from 96 or 384 well microtitre plates into a Caliper LabChip. The data signature is generated by the shift in mobility of non-phosphorylated peptide substrates and phosphorylated products by electrophoresis in the chip and detected via LED induced fluorescence (Figure 1). The height of the fluorescent signal reveals the extent of the reaction (Figure 2). Data is analyzed using LabChip EZ Reader software, which calculates the relative heights of the substrate and product peaks, and reports the product/(product+substrate) peak ratio (P/(P+S)). LabChip EZ Reader supports 4-sipper chips and is designed for compound profiling using ProfilerPro kit plates. LabChip EZ Reader II supports both 4 and 12-sipper chips making this the ideal instrument for secondary screening and MOA.

Real-Time Kinetic Analysis

The flexibility of the LabChip EZ Reader Series allows its use throughout the drug discovery process including assay development, primary screening, selectivity screening, generation of Structure-Activity Relationships (SARs) and Mechanism of Action (MOA). Most important therapeutic enzymatic targets such as kinases, proteases, phosphatases, histone deacetylases (HDAC), phosphodiesterases (PDE), and acyl-transferases can be developed using the LabChip EZ Readers. Real-Time Kinetic Data Mechanism of Action Studies Kinase Profiling Reproducable High Quality Data No Radioactivity No Antibodies

Figure 1. Shift in mobility of non-phosphorylated peptide substrate and phosphorylated product when separated in the chip by electrophoresis and detected via LED induced fluorescence used for HDAC assay development.

Figure 2. Height of the signal reveals the extent of the reaction.

LabChip EZ Reader Series

A Truer Understanding of Enzyme Behavior

Once compounds are identified, Mechanism of Action (MOA) studies are necessary to characterize and compare the potential drug candidates. The ability to follow reaction rates in real time greatly facilitates the determination of inhibition mechanisms and the calculation of disassociation constants. Characterization of enzyme activity through accurate determination of substrate Km and inhibitor Ki requires the ability to measure the rates of product formed as a result of an enzymatic reaction. Many of the methodologies used Determination of to endpoint assays resulting in apparent kinetic for identifying active compounds that modulate activity are limited ATP-Competitive Inhibition measurements. Using LabChip EZ Reader II, researchers may monitor enzyme reactions over time, resulting in more detailed data using less reagents.
Determination of ATP-Competitive Inhibition

Advantages of Microfluidic Technology with Real-Time Kinetic Analysis

Assay development and optimization is simplified and accelerated by the ability to follow reactions in real time. Initial rates are used to calculate actual Km and Ki values using real-time kinetics. Enzyme reaction linearity is precisely measured. Measurement of both product and substrate concentration improves data quality by providing: - Ratiometric data analysis - Lower false positive and negative artifacts - Detection of low potency inhibitors
Irreversibility of ERBB4 Inhibition

Irreversibility of ERBB4 Inhibition

LabChip EZ Reader Series Supports ProfilerPro Kinase Profiling Kits

Profile up to 12 compounds against 48 kinases in one day... in your lab!
Simple, Easy & Robust... ProfilerPro kits consist of
a matched pairs of 384 well kinase assay and substrate plates. The assay plates each contain 24 different kinases, pre-dispensed and frozen. The substrate plates contain the appropriate fluorescent labeled peptides and ATP. The final ATP concentration in each reaction is at the ATP Km. The kit also contains all reagents required and complete protocols.

LabChip EZ Reader
Height Width Depth Weight Power PC Controller Monitor

Specifications
464 mm (18.28 in) 472 mm (18.60 in) 649 mm (25.57 in) 45.5 kg (100 lbs) 100/110/230 VAC 50-60 Hz 2.4/2.0/2.0 AMPS 115/230 VAC 50-60 Hz 5.0/2.5 AMPS 100/240 VAC 1.5 AMPS Note: Adequate space of 200 mm (8 in) must be provided in front of instrument for plate loading. Right hand side of the instrument must provide sufficient clearance to allow for reagent access.

472 mm (18.60 in)

649 mm (25.57 in)

464 mm (18.28 in)

Front View

Side View

Part Numbers
123224 122919 760407 760404 760373 760374 760367 760394 LabChip EZ Reader and Controller LabChip EZ Reader II and Controller LabChip EZ Reader and LabChip EZ Reader II 4 Sipper Chip LabChip EZ Reader II 12 Sipper Chip ProfilerPro Kinase Panel Kit - plate 1 ProfilerPro Kinase Panel Kit - plate 2 Separation Buffer (400 mL) ProfilerPro Four Sipper Chip with 3X 400 mL Separation Buffer

Corporate Headquarters 68 Elm Street Hopkinton, MA 01748-1668 Tel: 1.508.435.9500 / 1.877.LabChip Fax: 1.508-435-3439 Email: cust.support@caliperLS.com www.caliperLS.com
2007 Caliper Life Sciences. All rights reserved. Caliper, LabChip, EZ Reader are tradenames and/or trademarks of Caliper Life Sciences, Inc. EZ-BR-01 Aug07