Hongwei Yuan, PH HONGWEI YUAN, PH.D. 32423 Yosemite Lane, Temecula, CA 92592. hy13b0912@westpost.

net, (C) 951-312-7188 PROFESSIONAL SUMMARY * Over 10 years of immunoassay and protein purification and 7 years of cell biology and molecular biology working experience in both industry and academic. * Performed and supervised method development and validation for protein expression, protein purification and conjugation, in vitro diagnostic (IVD) immunoassay test, and cell based assay. * Solid experience in cell and molecular biology, mammalian cell culture, in vivo animal study, and immunoassays. * Experience in the release test of raw materials, finished products and stability samples. * Extensive knowledge of cGMP and FDA guidelines. Played key role in several drug abuse immunoassay test submissions and FDAas inspections. * Strong technical strength in DNA and RNA techniques (cloning, expression, RT-PCR, etc.) chromatography (HPLC, FPLC, ion exchange, affinity purification, etc), confocal microscope and other instruments. * Wrote SOPs, validation protocols and reports. Proven history of publications in scientific journals and presentations at scientific meetings. * Proven skills of the ability to plan and execute projects. * Expertise in applying quantitative methods: analyzes data, evaluates results, forms conclusions, and provides/implements process or document improvements. * Strong trouble-shooting capability, analytical skills and attention to detail. * Excellent organizational and documentation skills with multi-tasking ability. *

Experience of supervising junior research staff. * Good team player that can work independently. TECHNIQUES HPLC, FPLC, affinity purification, protein purification and conjugation, Western Blotting, preparative electrophoresis, SDS-PAGE, ELISA, RIA, staff supervisory, Microsoft office programs, Imaging and scanning software, Sigma Plot, Graph Pad, Photoshop, bioreactors, Biacore X100, Atto Pathway HT. In Vitro diagnostic device, lateral flow immunoassay, multiplex bead array, design control, projects management, Mammalian cell culture, cell based assay, in vivo animal experiment study, stem cell biology, RT-PCR, real time PCR, FACS, ELISPOT, FISH, DNA and RNA techniques, transfection, antibody preparation and characterization, immunolabeling, immunofluorescent assay, immunohistochemistry, light and fluorescent microscopy, confocal microscopy, chromatography EMPLOYMENT BACKGROUND/TRANSFERABLE ACCOMPLISHMENTS INTERNATIONAL IMMUNOLOGY CORPORATION, MURRIETA, CA MAY 2010 TO 2011 STAFF TECHNOLOGIST, Antigen Purification and Development. * Played key role in the purification of immunoglobulins and apolipoproteins using various chromatographic methods, such as ion-exchange, absorption, gel perfusion, and affinity purification. * Investigated and validated the methods for the activity assay of the purified protein from human serum. * Executed the instrument calibration and qualification. * Performed troubleshooting of protein chromatography and immunoassay methods. EMD CHEMICALS, INC. SAN DIEGO, CA FEBRUARY 2010 TO MAY 2010 SCIENTIST, Production Department * Assisted in process development and troubleshooting projects. * Extracted and purified recombinant proteins within predetermined time schedule. * Performed protein labeling with different dyes for purity and specificity immunoassay. *

Validated and troubled the SOPs. UNIVERSITY OF CALIFORNIA, RIVERSIDE, CA OCTOBER 2002 TO DECEMBER 2009 * Professor Manuela Martins-Green Laboratory is a wound healing lab that studies the mechanisms of normal and abnormal wound healing processes, including cigarette smoke induced atherosclerosis. POST DOCTORAL SCHOLAR September 2008 To December 2009 * Explored AMP kinase agonist in the alleviation of smoke induced atherosclerosis and liver damage. * Discovered that pomegranate juice can prevent prostate cancer metastasis by affecting cell migration, using cultured prostate cancer cell lines. * Conducted the effects of insulin and SDF1 on wound healing process in mouse model. * Researched the function of thymosin 4 in combination with anti-inflammatory agents in reversing asecond-handa cigarette smoke-induced impairment of cornea wound healing. * Constructed a 3D human artery model for early atherosclerosis research, using cultured primary human aorta endothelial cells, smooth muscle cells and fibroblasts. * Trained and supervised 5 lab members. GRADUATE STUDENT RESEARCHER October 2002 To August 2008 * Established the correlation between cigarette smoke toxicity and atherosclerosis in mouse model. * Elucidated the mechanism of SSW inducing fatty liver disease using cultured hepatocyte cell line. * Clarified that periendothelial cells in microvessels arise by differentiation from fibroblasts, not from stem cells. SYNTRON BIORESEARCH, INC., CARLSBAD, CA MAY 1997 TO SEPTEMBER 2002 RESEARCH SCIENTIST, SUPERVISOR

A research, development and manufacturing company of rapid in vitro immunodiagnostic test systems. * Performed troubleshooting and trained new chemists * Extracted and purified recombinant antigens that increased the sensitivity and accuracy of the tests. * Generated methods for making enzyme-antibody as well as small molecule-antibody conjugates. * Developed Quick-Pac whole blood test, including Mononucleosis, HCV, Drug abuse tests, etc. * Supervised instrument calibration and qualification program. * Played key role in FDA 510(k) submissions for IVD drug abuse tests. * Wrote and reviewed SOPs. INSTITUTE OF RADIATION MEDICINE, CHINESE ACADEMY OF MEDICAL SCIENCES, Tianjin, China SEPTEMBER 1989 TO MARCH1997 A leading research institute of experimental nuclear medicine and radiation medicine in China, with focus on using immunoassay technologies and methodologies in nuclear medicine. GROUP LEADER, May 1995 To March 1997 * Motivated and supervised five researchers. * Responsible for planning, managing, and reporting the progress of two research projects. * Engineered the ScFv fragment of antibody for the in vivo radio immunoassay. * Increased the image quality of radio immunoimmaging methods for the diagnosis of acute myocardial infarction and thrombi. * Conducted antibody directed thrombolysis and increased the efficiency by 2.7 times in mouse model. RESEARCH ASSOCIATE, September 1989 To May 1995

* Prepared monoclonal antibodies and characterized them using ELISA, electrophoresis, etc. * Operated and maintained HPLC, FPLC chromatography system for protein purification. * Performed and supervised over 10 method validations/verifications for the purified antigens and antibodies. * Studied radio labeling methods of the antibodies for radio immunoassay test kits. EDUCATION Ph. D.,Cell, Molecular, and Developmental Biology, University of California, Riverside, CA (2008). B. Sc., Biochemistry, Shandong University,Jinan, China. (1989). HONORS AND AWARDS * Outstanding Teaching Assistant, University of California, Riverside, 2006 - 2007. * Dissertation Research Grant, University of California, Riverside, 2006 - 2007 * Poster award, California Tissue Engineering Conference, September 2006 * Deans Fellowship package, University of California, Riverside, 2002 - 2007 PUBLICATIONS/ARTCILES SELECTED PUBLICATIONS * Dai, Q.*, Yuan, H.*, & Martins-Green, M. A 3-D multi-cellular human arterial wall culture system for the study of blood vessel biology and disease. Submitted to Int J Biochem Cell Biol. *Equal contribution * Yuan, H., Ma,C., Moinet, L., Sato, N. and Martins-Green, M. Thymosin 4 in combination with anti-inflammatory agents reverses asecond-handa cigarette smoke-induced impairment of cornea wound healing. Invest Ophthalmol Vis Sci. 2010, 51:2424-2435. *

Yuan, H., Shyy, J. Y., & Martins-Green, M. Second-hand smoke stimulates lipid accumulation in the liver by modulating AMPK and SREBP-1. J Hepatol 2009, 51: 535-547. Published with editorial. * Njauw, C. N.*, Yuan, H. *, Zheng, L., et al. Origin of periendothelial cells in microvessels derived from human microvascular endothelial cells. Int J Biochem Cell Biol 2008, 40: 710-720. *Equal contribution * Yuan, H., Wong, L. S., Bhattacharya, M., et al. The effects of second-hand smoke on biological processes important in atherogenesis. BMC Cardiovasc Disord 2007, 7:1.