NAME:

KEV
Exam 2

Bubble in your answersheetwith the correct choicein eachcase(45 points) the 1. On the graphs below,which line represents additionof a NON-COMPETITIVE INHIBITOR to the originalcondition(line 1)?

binding? 2. Which statement NOT true of the sequential is modelof allosteric on a. a subunitcanexist in eithertenseor relaxedforms depending the circumstances b. binding of an inhibitor to one subunitmakesbinding of inhibitor to other subunits easier.but doesnot fullv convertthe other subunitsto the tenseform -.A' bindingof activator othersubunits easier, to makes to f c) bindingof activator onesubunit Yut doesnot affectsubstrate bindingin any of the units the binding may be saidto oostabilize" relaxedform d. activatorand substrate oostabilize" tense the form e. inhibitorbindingmay be saidto 3. Whichof thefollowingis mostlikely to be phosphorylated? (CHz)NHr*) d. Ala (R: CH3) ALys (R: : CH2OH) e. they areall equallylikely to be (b)Ser (R phosphorylated E cly (R: H) 4. a. b. c. Which is an saturated fatty acid? oieicacid:18:l-Ae acid;18:2-Ae'12 linoleic 12' linolenicacid;18:3-Ae' 15 rr' d. arachidonic acid;20:4-A5'8' 14 6) norr. of the above

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from cholestelol UV light? via 5. Which vitamin is biosynthesized a. vitaminA @vitamin D e. vitamin E b. vitamin B c. vitamin C 6. Which is an exampleof primary activetransport? across membrane of a cell out a. COzmoves the into b. Ozmovesacross membrane a cell the proteinpermease a cell into c. glucose moveswith the assistance themembrane of ([glu]"* > [glu]'") ([glu]*t < [glu]in, and is into d. glucose co-transported a cell via a Na-/glu symporter
([Na-]ou, > [Na-]in)

out @Nu* is transported of a cell via Na*/K*-ATPase

7. Which of the diagramsbelow represents D-glucose? a.

| H-oH

cHo

b.

qHo

Ho-tH

gHo

HtoH Hotn n-*-oH r-*-or

CH2OH

d.

cHo

e.

H--Fon Ho-l-r-r no*n ,-J-on

CHrOH

i-

| H-1-oH
Hc I H-J-oH

cHo

n - -|--onn-forl r,#t I I n*on no*n n**

CH2OH

lllll

no*n
CH2OH

no*"
CH2OH

phosphorylase kinase? 8. Which of the following activates a. cAMP @ proteinkinase phosphorylase e. UDP-glucose b, glycogen synthase c. glycogen (glycosidic linkageis galactose-B-1,4-glucose), of which 9. In the disaccharide cx-lactose the followin g is ll1ftl trae? d. neithermonosaccharide can a" the galactose in the o position is muiargtatg is @m. glucose in the crposition e- bothmonosaccharides mutarotate can i. the anomeric the crpositionis in lockedinto place 10.Which species the electon donorin the reactionshown: is NAD* + CH:CHzOH-r NADH + H* + CH3CHO d. NAD* a. CH:CHO e. insufficientinformation b. NADH

@urcHroH

BCH 361 Hendrickson metabolism? 11.Which general statement TRUE regarding is and reactions is a. catabolism the sumof metabolic anabolic process which releases energy is a b. anabolism generally reductive which requiresenergy c. catabolismis generallya reductiveprocess which releases energy @catabolism is generallyan oxidativeprocess which requires energy is an e. anabolism generally oxidativeprocess 12.For a system equilibrium,which of the following MUST be true? at
a .AG > 0

b .a G< 0

d. AGo'= 0 e. AG"' < 0

@lc: o
13.The formationof which intermediate occurswith a large,negative in glycolysis? AG d, 3-phosphoglycerate a. fructose-6-phosphate (6) glucose-6-phosphate e. glyceraldehyde-3-phosphate phosphoenolpyruvate i of 14.Which is NOT a productof the net reaction glycolysis? a. 2ATP @itu"tut" Y ail of these products b. 2 NADH are c. 2 pyruvate NOT a typeof regulation phosphofructokinase? 15.Which does of a. activationby fructose-6-phosphate d. activatedby AMP b. inhibited by citrate oftheseareaccurate O "ll c. inhibitedby ATP Answer.thequestions below; showcalculationswhere relevantfor partial credit (below),draw c-lactose(galactose-B-1,4-glucose). (10 points)Givengalactose 1.

BCH 361 Hendrickson plot 2. (15 points)Giventhe Lineweaver-Burk shownbelow,calculate andVmax Km for eachcondition. Indicatewhetherthe inhibitor is competitive non-competitive. or plot Sketch Michaelis-Menton that corresponds the data. (Note: your MM plot does a to not needto includenumbers;simply indicateKm andVmax, andget the basicshape of plot correct). Labelall axes. the
Inhibitor

o(t G
---

ntercept -5.31 --

-' V nr . * ' t/y-,nt -- 8 ,l q r t o -l/.-' t = -',/,o,Lq:

( ,0 1 ,q)

K^

A 1 .7 Lx t o-L (, u0, ?

lN lhg

- l/- s.st= l.81xlo-r (o -ttt\ Lr^'

xlo-L Vuar = 8.11

I cr*Pennvt
ffrt tou.

o?-ro
1rul".rb

V
K^ K^(t"t",5) D^S)

fsl

BCH 361 Hendrickson glucose makeglucose-6-phosphate. to 3. (15 points)ATP canbe usedto phosphorylate glven the following: What is the net reaction? What is AGo' for the reaction, + glucose Pi + glucose-6-phosphate, AGo'= 13.8kJ/mol ADP + Pi + ATP,AGo':30.5 kJ/mol R = 8 . 3 1 4 Jl mo l K .T =2 9 8 K

> AT? t SLr,tcos4

bGl rrn,' l3.t .5l*l LS/,r,toL -16'+ lc1l"+ol ' - 3o:5

'

.i ;t:

"r '

o;*;*

BCH 361 Hendrickson below. 4. (15 points) Fill in the blankintermediates ADDITIONALLY: and l) Providethe names thethreemajorenzrymes, clearlyindicatewhich step of they catalyze 2) Indicatethe step(s)whereATP is required,andhow manyATP arerequiredPER GLUCOSE whereATP is produced, how manyATP areproduced and 3; Indicatethe step(s) PERGLUCOSE and 4) Indicatethe step(s)whereNADH is produced, how manyNADH are produced PERGLUCOSE

Lrrt,,) / PN
AT

gluc.'sc

f;("P

Vvroutnftoa-

[tr*> nNP /
/\

AtP --

t FaP
r

i
-----+

prn

lhrctose-I .6-bisphosphatc

bHrflp

grrccrartrcrrrdc-.1-ph.r'h.rr. NA,S* (

| \. lal*t^) v v

A,5? Pil)r'

' GlYcua{+-\'3'btsPho.S

I Y

L>

Gr*,-)

^JAs\1

| +
-phosplxrg lvccratc

ccrrrtc !-phosplroglr t

I
I

phosphocnolpr'rur'atc -

trt*A Hl

Linnr*e i pu.
PYnrvruK