You are on page 1of 1

Qualitative Errors in HPTLC

http://www.interchromforum.com/html/ql_err_hptlc.html

Home

The qualitative errors in HPTLC are less critical as in HPLC althoug chromatographic separation power is much smaller than available for mo latter offers a total peak separation capacity of around 50 (baseline sepa Standard HPTLC reaches a total peak separation capacity of about 15. Thus spot overlapping exists most often.

Lets have a look onto the planar chromatogram l beauty of a visualization by fluorescence. It show information. But in case of wider ranging concent immediately reach limits especially when these s partially overlap. There is of course full spectral signal power avail enhance even very weak peak signals which is no elution chromatography as in HPLC or with forced

This page concentrates on a classical systematic error in HPTLC: the falsified quality value Rf. By the Rf value HPTLC users express qualitative re The linear Rf value for substance x = s / f See figure 1 below.

f = length of the mobile phase path developed from start to front. s = the path length the substance x was moved until the HPTLC run stopped.

The main reason to concentrate here in all details on the Rf value error is the fact allows to use HPTLC-k-values which are comparable with HPLC-k-values. If the m phases in HPLC are exactly equal in HPTLC, the HPLC-k-value corresponds with th Although both differ by value it becomes evident that the two liquid chromatograp support each other qualitatively. Due to the very limited separation efficiency of b modern micro gas chromatography a both sides support of these sister techniques serious systematic qualitative errors. As the life combination of HPLC with HPTLC bridge to gap error trouble, an equal language for both techniques is mandatory fails.

For qualitative HPTLC and qualitative HPLC isocratic separation techniques are pre qualitative HPLC data are time or volume based. The best quality value is k = resi stationary phase over the residence time in the mobile phase: k = ts / tm .

It is not immediately visible, that HPTLC data are also time based. But this is show

Especially helpful in HPTLC is the comparison and the simultaneous use of linear, circular HPTLC. Circular techniques are not available in HPLC. NOTE that HPLC is substances which chemisorb or very strongly adsorb in the stationary phase, that HPLC-k-values of over 1000. Especially circular HPTLC with enlarged separation p with higher HPTLC-k-values can help HPLC users. Substances with very large HPLC-k-values r the HPLC column, that is: remain invisible. HPTLC allows to make substances with k-values visible Whilst a HPTLC information about substances with extreme k-valu quantitation techniques towards quantitative inner standard modes, anti circular H avoid HPLC errors hidden under peaks with very small k-values. Circular HPTLC techniques are important for HPLC users . NOTE: -PLC IS CIRCULAR. A complete top level HPTLC working place needs quit
1 of 1 16/01/2555 18:25

You might also like