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General Information
Glycated proteins are formed post-translationally from the slow, nonenzymatic reaction between glucose and amino groups on proteins. For hemoglobin, the rate of synthesis of Hemoglobin A1c (HbA1c) is principally a function of the concentration of glucose to which the erythrocytes are exposed. HbA1c is a clinically useful index of mean glycemia during the preceding 120 days, the average life span of erythrocytes. Carefully controlled studies have documented a close relationship between the concentrations of HbA1c and mean glycemia, while routine determinations of blood glucose by patients or by their healthcare providers are not considered as reliable as HbA1c to quantify mean glycemia. Concentrations of other blood-based glycated proteins (e.g., glycated serum or plasma proteins, "fructosamine") also reflect mean glycemia, but over a much shorter time than HbA1c: 15-30 days and 60-120 days, respectively.
Diabetes
Principle
The i-CHROMATM HbA1c is based on fluorescecnce immunoassay technology. It uses competition immunodetection method. Whole blood from blood specimen is added to the mixture of hemolysis buffer and detection buffer which results in hemolysis of red blood cells. The mixture containing HbA1c from the hemolyzed red blood cells and fluorescence-labeled HbA1c peptides from detection buffer is loaded onto the sample well of Test Device and migrates the nitrocellulose matrix of test strip by capillary action. HbA1c of whole blood competes with fluorescence-labeled HbA1c peptides for binding sites on HbA1c antibodies of nitrocellulose matrix. Thus, the concentration of HbA1c antigen in blood specimen shows inversely proportional relationship with intensity of fluorescence of HbA1c-peptides. The result is displayed on i-CHROMATM Reader in units of percentage.
Diabetes
i-CHROMA
TM
Contents
Contents box
Test Device
1 ID chip
1 Manual
Press "Select"
10 times
Collect 5ul of sample directly from a fingertip or prepared specimen using a capillary tube.
Drop the sample containing capillary into the tube containing the mixture of hemolysis buffer and detection buffer. (from step #5)
the mixture of buffer and the sample blood has to be used within 30 seconds.
Shake the tube 10 times or more until the blood is completely out of collection capillary :
sample window
Hemoglobin window
the mixture onto the sample well 10 Transferslowly. of the Test Device
11
Collect 100ul of the mixture (of sample and buffers from step #8)
12
Transfer the mixture onto the Hemogloin Window of the Test Device slowly.
12 minutes
13
Wait 12 minutes.
14
Place the Test Device on the holder and push all way back.
15
Press "Select"