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Rin
[M]
++ +
ufb
feedback control error
plant
[log10 Hz]
output
start [mV]
0 -60
steady-state
target
+
gpas
start post-stim steady-state 100 m
gKdr
Biologically plausible models of control systems that underlie neuronal homeostasis require sensor mechanisms that are known to exist in the cell. Intracellular calcium concentration is often favoured because many calcium-dependent signalling pathways are present in neurones, and calcium buffers are known to interfere with homeostatic compensation in a variety of experimental settings (Turrigiano et al., 1994; Berridge et al., 2003; Davis, 2006). Error signals can be transformed by the control process in a variety of ways. Some of the possibilities are illustrated in the table (right). Homeostasis requires a negative feedback control; three common examples are proportional control (which delivers a signal proportional to the error signal), integral control (signal is proportional to the accumulated, or integrated error) and bang-bang control (which generates a fixed on or off signal once the error crosses a target threshold). All three mechanisms are biologically plausible, however integral control is favoured because it eliminates steady-state error. In addition, integral control can be implemented with a straightforward model of the dynamics of conductance regulation (outlined below). Bang-bang control is an idealized model of processes which exhibit threshold behaviour, and might plausibly describe processes such ion channel expression. We focussed on integral control, but explored bang-bang control in the context of noise.
m ho
profile path
t ic sta eo ?
Controller
Characteristics
Feedforward
Reaches target state if tuned, does not compensate for external disturbances. Moves toward target state but never reaches it. Partially compensates for external disturbances. Reaches target state with a lag and/or overshoot. Compensates for external disturbances Fast response but oscillates about target state. Compensates for external disturbances.
Proportional
Which controller?
The error signal and the control mechanism itself exist in a noisy environment. This is especially true in dendrites, which are subjected to a large amount of electrical noise and where noise due to the stochastic nature of biochemical signalling is substantial (Cannon et al., 2010; Kotaleski and Blackwell, 2010). We investigated the performance of an integral controller with the same parameters as that used in our neuronal model in the presence of noise (Ornstein-Uhlenbeck process, cut-off frequency = 100 Hz). Interestingly, the integral controllers performance compares poorly with a much cruder bang-bang controller under conditions of high noise (below) and exhibits far stronger frequency dependence (below right).
10
0
Integral
Bang-bang
We used a multi-compartment model of a reconstructed CA1 pyramidal cell (Migliore et al., 2005) implemented in NEURON (Hines and Carnevale, 1997). From this model, we kept the passive leak and voltage-gated sodium and (delayed-rectifying) potassium conductances and introduced simplified calcium dynamics. Membrane conductances were modified to model activity-dependent homeostasis by introducing first-order calcium-dependence in the maximal conductance with a slow timeconstant (100 s). The details of the model are summarized below. Standard Hodgkin-Huxley style model:
dV Cm = g pas (V E pas ) + g Na (V ) + g Kdr (V ) dt
integral
The model
bang-bang
no noise
noise present
SNR = 2 (~ 3 dB)
10
r ( x ) = g r 10kr x ,
6 r ( x ) 2
Mean-squared error
10
-5
Mean-squared error
Controller transformation:
10
-10
10
-10
Conductance regulation:
dg r = r ([Ca]i Cr ) (implicit integral control) dt
Ca = 100 ms mM1
C = 10 4 mM Veq = 65 mV E pas = 60 mV
10
10
-15
-20
10
-2
10
-1
10
10
10
-5
10
-3
10
-1
10
Random excitatory synaptic input was introduced to the apical dendrites at a mean (Poisson) rate of 10 Hz and with a lognormal distribution in conductance (mean = SD = 100 nS, Erev = 0 mV). The figure (below) shows a sample of activity, with the dendrites of the model cell pseudo-coloured according to membrane potential
Noise/signal ratio
40
[mV]
-60
Vm
10 ms
References
Berridge MJ, Bootman MD & Roderick HL. (2003) Nat Rev Mol Cell Biol 4, 517-529. Cannon RC, O'Donnell C & Nolan MF. (2010) Plos Comp Biol. 6. Davis GW. (2006) Annu Rev Neurosci 29, 307-323. Hines M & Carnevale T. (1997) Neural Computation 9, 11791209 Kotaleski JH, Blackwell KT (2010) Nat Rev Neurosci. 11:239. Migliore M, Ferrante M & Ascoli G. (2005) J. Neurophysiol. 94:6. Turrigiano G, Abbott LF & Marder E. (1994) Science. 264, 974-977.
[Ca]i C