Professional Documents
Culture Documents
VECTORS
RESTRICTION ENDONUCLEASES
Self-replicating DNA molecule that has the ability to carry another foreign DNA molecule into a host cell. Purpose - insert a fragment of DNA into a vector so that it can be replicated and grown into quantities suitable for study.
PLASMID
Chloramphenicol Acetyltransferase (CAT) Firefly Luciferase -Galactosidase Secreted Alkaline Phosphatase (SEAP) Human Growth Hormone (hGH) -Glucuronidase (GUS) Green Fluorescent Protein (GFP)
GENOMIC LIBRARY
LIBRARIES
cDNA LIBRARY
DNA complementary to mRNA - only the expressed genes of a particular cell.
8e.devbio.com/im ages/ch04/0405fi g2.jpg
EXPRESSION LIBRARY
GEL ELECTROPHORESIS
www.cbs.dtu.dk/staff/dave/roanoke/fig5_33.jpg
http://www.mun.ca/biology/scarr/Lab_5_gel_1999.gif
http://fig.cox.miami.edu/~cmallery/2 55/255tech/mcb3.32.SDS.elect.jpg Hall and Giaccia, Radiobiology for the Radiologist, 2006
RT PCR
NORTHEN BLOT
http://www.mcmaster.ca/inabis98/higuchi/noda0326/f3small.gif
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FAR-WESTERN BLOTTING
WESTERN BLOT
http://www.bioon.com/experiment/UploadFiles/200408/20040803131929294.gif
EXPRESSION MICROARRAYS
www.Affymetrix.com
www.Affymetrix.com
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www.Affymetri x.com
www.Affymetr ix.com
www.Affyme trix.com
www.Affymetrix.com
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www.Aff ymetrix.c om
SNP
http://ww w.ogt.co. uk/image s/humangeneexpressio n.gif www.seq.ubc.ca/deco de.ne-icelands-dna/
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PERSONALIZED MEDICINE
The use of detailed information about a patient's genotype in order to select a medication, therapy or preventative measure that is particularly suited specifically to that patient.
www.sanger.ac.uk
SNP ASSAYS
National.diagnostics.combined/images/2_3_2/gif
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MOLECULAR BEACONS
5 Nuclease TaqMan Assay
Clinical Science www.clinsci.org. Clin. www.clinsci.org. Clin. Sci. (2005) 109, 365Sci. 365379
Affymetrix
Illumina
www.Affymetrix.com
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http://www.biken.osaka-u.ac.jp/act/act_okabe_e.php
KNOCKOUT MICE
http://64.202.120.86/upload/image/articles/2006/biopen/biopen-elisa-schematic.jpg
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IMMUNOPRECIPITATION
www.biochem.arizona.edu/.../Am64./8a.gif
www.molecularstation.com
CHROMATIN IMMUNOPRECIPITATION
ChIP
ChIP-on-chip
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DNase I Footprinting
siRNA
miRNA
www.scq.ubc.ca/wpcontent/siRNA.gif
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COMET ASSAY
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