BT Syllabus - 2009

UNIVERSITY OF CALICUT
B.TECH BIOTECHNOLOGY CURRICULUM And SYLLABUS

FROM 2009 ADMISSION ONWARDS
(1&2 SEMESTER 8TH SEMESTER)
B. TECH.
BIOTECHNOLOGY
1 & 2 SEMESTER
(Common for All Branches)
Hours / week Code Subject
L T P/D
Marks
Internal Endsem
End-sem durationhours
Credits
EN09 101 EN09 102 EN09 103
Engineering Mathematics I Engineering Mathematics II Engineering Physics
2 2 2 2 2 2 2 0 1 -
1 1 1 1 1 -
1 1 3 1 2 2 10
30 30 30 50 30 50 30 30 30 30 50 50 50
70 70 70 50 70 50 70 70 70 70 50 50 50
3 3 3 3 3 3 3 3 3 3 3 3 3
4 4 3 1 3 1 4 4 4 3 3 2 2 38
EN09 103(P) Physics Lab EN09 104 Engineering Chemistry
EN09 104(P) Chemistry Lab EN09 105 EN09 106 EN09 107 EN09 108 Engineering Mechanics Basics of Civil and Mechanical Engg. Basics of Electrical, Electronics & Communication Engg. Engineering Graphics
EN09 109(P) Computer Programming in C EN09 110(P) Mechanical Workshops EN09 111(P) Electrical and Civil Workshops Total
15 5
B. TECH. BIOTECHNOLOGY THIRD SEMESTER

Hours / week Code Subject L T Marks Semesterend Semester-end Examination duration hours Credits
P/D Internal
EN 09 301 EN 09 302 BT 09 303 BT 09 304 BT 09 305 BT 09 306 BT 09 307 (P) BT 09 308 (P)
Engineering Mathematics III Humanities &Communication skills Fluid flow operations Microbiology Biochemistry I Bioprocess calculations Microbiology Lab Biochemistry Lab Total
3 2 4 3 3 3
1 1 1 1 1 1
3 3
30 30 30 30 30 30 50 50
70 70 70 70 70 70 50 50
3 3 3 3 3 3 3 3
4 3 5 4 4 4 2 2 28
18
B. TECH. BIOTECHNOLOGY FOURTH SEMESTER

Code Subject L Hours/week T P/D Semester-end Marks Inter- Semester- Examination nal duration hours end Credits
EN 09 401 EN 09 402 BT 09 403 BT 09 404 BT 09 405 BT 09 406 BT 09 407 (P) BT 09 408 (P)
Engineering Mathematics IV Environmental Science Chemical Reaction Engg. Biochemistry II Cell Biology Mechanical Operations Fluid Flow and Mechanical Operations lab Bioanalytical techniques Lab Total
3 2 4 3 3 3 -
1 1 1 1 1 1 -
3 3
30 30 30 30 30 30 50 50
70 70 70 70 70 70 50 50
3 3 3 3 3 3 3 3
4 3 5 4 4 4 2 2 28
18
B. TECH. BIOTECHNOLOGY FIFTH SEMESTER

Hours/week Code Subject L T Semester-end Semester- Examination Credits P/D Interduration hours nal end Marks
BT 09 501 BT 09 502 BT 09 503 BT 09 504 BT 09 505 BT 09 506
Mass Transfer Operations Molecular Biology Enzyme Science and Engg Thermodynamics & Heat Transfer Operations Bioinformatics Process Instrumentation
4 3 3 3 3 2 18
1 1 1 1 1 1 6
3 3 6
30 30 30 30 30 30 50 50
70 70 70 70 70 70 50 50
3 3 3 3 3 3 3 3
5 4 4 4 4 3 2 2 28
BT 09 507 (P) Heat and Mass Transfer Lab BT 09 508 (P) Bioprocess Engg. Lab Total
B. TECH. BIOTECHNOLOGY SIXTH SEMESTER

Hours/week Code Subject L T Marks Semester-end Semester- Examination P/D Internal duration hours en d Credits
BT 09 601 BT 09 602 BT 09 603 BT 09 604 BT 09 605 BT 09 LXX
Genetic Engineering Bioprocess Engineering. Financial Management & Cost Estimation of Process Industries Process dynamics & control Food Biotechnology
4 3 3 3 2 3 18
1 1 1 1 1 1 6
30 30 30 30 30 30 50 50
70 70 70 70 70 70
3 3 3 3 3 3
5 4 4 4 3 4 2 2 28
Elective I Molecular Biology & Genetic BT 09 607 (P) Engg. Lab BT 09 608 (P) Bioinformatics Lab Total Elective I
1 2 3 4 5 BT BT BT BT BT 09 09 09 09 09 L 01 L 02 L03 L 04 L 05 Plant Biotechnology Animal Biotechnology Protein Engineering Metabolic Engineering Genomics & Proteomics
3 3 6
50 50
3 3
B. TECH.
BIOTECHNOLOGY
SEVENTH SEMESTER
Hours / week Code Subject L T Marks Credits Semester-end Semester- Examination P/D Internal duration hours end
BT 09 701 BT 09 702 BT 09 703 BT 09 704 BT 09LXX BT 09 LXX
Bioprocess Plant Design Downstream Processing Environmental Engineering Immunology & Immunotechnology Elective II Elective III
4 3 2 2 3 3 17
1 1 1 1 1 1 6
3 3 1 7
30 30 30 30 30 30 50 50 50
70 70 70 70 70 70 50 50
3 3 3 3 3 3 3 3
5 4 3 3 4 4 2 2 1 28
BT 09 707 (P) Downstream processing lab Reaction Engg. & Process BT 09 708 (P) Control Lab BT 09 709 (P) Project Total
B. TECH.
BIOTECHNOLOGY
EIGHTH SEMESTER
Hours/week Code Subject L T Marks Semester-end Semester- Examination Credits P/D Interduration hours nal end
BT 09 801 BT 09 802 BT 09 LXX BT 09 LXX
Plant operations, safety and safety Industrial Biotechnology and Biopharmaceuticals Elective IV Elective V
4 3 3 2 -
1 1 1 1 4
3 11 14
30 30 30 30 100 100 -
70 70 70 70
3 3 3 3 3
5 3 4 4 2 7 3 28
BT 09 805(P) Seminar BT 09 806(P) Project BT 09 807 Viva-voce Total
100
12
ELECTIVES
For ELECTIVE II ELECTIVE III ELECTIVE IV ELECTIVE V
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
BT 09 L06 Gene and stem cell therapy BT 09 L07 Molecular diagnostics BT 09 L08 Molecular pathogenesis BT 09 L09 Membrane separation technology BT 09 L10 Recombinant DNA technology BT 09 L11 DNA microarray technology BT 09 L12 Cancer biology BT 09 L13 Structural biology BT 09 L14 Molecular modelling and drug design BT 09 L15 Biosensors and bioinstrumentation BT 09 L16 Molecualar medicine BT 09 L17 Hazardous waste management BT 09 L18 Analytical techniques in biotechnology BT 09 L19 Energy Engineering BT 09 L20 Transport phenomena in bioprocess systems BT 09 L21 Design and analysis of bioreactors BT 09 L22 Modelling and simulation of process plants BT 09 L23 Nanobiotechnology BT 09 L24 Bioethics & Intellectual Property Rights BT 09 L25 Biomaterials
GLOBAL ELECTIVES FROM OTHER BRANCHES

1 2 3 4 5 6 7 8 9 10 ME 09 ME 09 PE PE PE CS CS CH CH IT 09 09 09 09 09 09 09 09 L 24 Industrial safety Engineering L 25 Energy engineering and management L23 Total quality management L 24 Industrial psychology L25 Entrepreneurship L23 Simulation and modeling L24 Computer based numerical methods L23 Nano materials and nanotechnology L25 Project engineering L24 Management information systems
EN09 101: Engineering Mathematics I

(Common for all branches) Teaching scheme 2 hours lecture and 1 hour tutorial per week Credits: 4
Objectives This course is addressed to those who intend to apply the subject at the proper place and time, while keeping him/her aware to the needs of the society where he/she can lend his/her expert service, and also to those who can be useful to the community without even going through the formal process of drilling through rigorous treatment of mathematics. Module I: Differential Calculus (15 hours) Indeterminate forms LHopitals rule Radius of curvature (Only Cartesian form) Center of curvature - Evolute Functions of more than one variables - Idea of Partial Differentiation Eulers theorem for Homogeneous functions Chain rule of Partial differentiation Application in errors and Approximations Change of variables Jacobians Maxima and Minima of functions of two variables Method of Legrange multipliers. Module II: Infinite Series (15 hours) Definition of Convergence and Divergence of Infinite series Ratio test Comparison test Raabes test Root test Series of positive and negative terms Absolute convergence Test for Alternating series Power series Interval of Convergence Taylors and Maclaurins series expansion of functions Leibnitz formula for the nth derivative of product of two functions Its use in Taylors and Maclaurins series expansions. Module III: Matrices (15 hours) Rank of a matrix Reduction of a matrix to Normal and Echelon forms System of Linear equations Consistency of System of Linear Equations Gauss elimination method System of Homogeneous Linear equations Solution of System of Homogeneous Linear equations by Gauss elimination method Eigen Values and Eigen Vectors Cayley-Hamilton Theorem Diagonalisation of a matrix using Eigen vectors Quadratic forms Definite, Semi-definite and Indefinite forms Matrix associated with a quadratic form Reduction to Canonical form by orthogonal transformation. Module IV: Fourier Series and Harmonic Analysis (15 hours) Periodic functions Trigonometric series Fourier series Euler Formulae Even and Odd functions Fourier series of Even and Odd functions Functions having arbitrary period Fourier series of Functions having arbitrary period Half-range expansions Numerical method for determining Fourier coefficients Harmonic Analysis. Reference books 1. Michael D Greenberg, Advanced Engineering Mathematics (2 nd Edition), Pearson Education Asia. 2. Wylie C.R and L.C. Barrent, Advanced Engineering Mathematics, McGraw Hill. 3. Kreyzig E., Advanced Engineering Mathematics, Wiley eastern. 4. Piskunov N., Differential and Integral calculus, MIR Publishers. 5. Ayres F., Matrices, Schaums Outline Series, McGraw Hill. 6. Sastry S.S., Advanced Engineering Mathematics-Vol. I and II., Prentice Hall of India. 7. Glyn James., Advanced Engineering Mathematics, 3/e, Pearson Education Asia. 8. Dr.ChandraMohan,Dr.Varegheese Philip, Engineering Mathematics I,II,III & IV , Sanguine Technical Publishers. 9. Peter V ONeil, Advanced Engineering Mathematics, Thomson India Edition. Internal Continuous Assessment (Maximum Marks-30) 60% - Degree Programme Syllabi - Combined First & Second Semesters B.Tech. Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc.
9
10% - Regularity in the class
10
University Examination Pattern PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions 4 x 5 marks=20 marks
Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions Two questions from each module with choice to answer one question. Maximum Total Marks: 70 4 x 10 marks=40 marks
EN09 102: Engineering Mathematics II

Objectives Mathematics is a necessary avenue to scientific knowledge which opens new vistas of mental activity. A sound knowledge of engineering mathematics is a sine qua non for the modern engineer to attain new heights in all aspects of engineering practice. This course provides the student with plentiful opportunities to work with and apply the concepts, and to build skills and experience in mathematical reasoning and engineering problem solving.
Module I: Ordinary Differential Equations (15 hours) Equations of first order Separable, Homogeneous and Linear Exact Equations Orthogonal trajectories Linear second order equations Homogeneous Linear equation of second order with constant coefficients fundamental system of solutions Solutions of the general Linear equations of second order with constant coefficients method of variation of parameters Cauchys equation. Module II: Laplace transforms (15 hours) Gamma and Beta functions Definitions and simple properties Laplace transform Inverse Laplace transform shifting theorems Transforms of derivatives and integrals Differentiation and integration of transforms Convolution theorem (No proof) Transform of Unit step function Transform of Impulse function transforms of periodic functions Solution of ordinary Differential equations using Laplace transform. Module III: Vector Differential Calculus (15 hours) Vector function of a Single Variable Differentiation of vector functions Scalar and Vector fields Gradient of Scalar fields Divergence and Curl of Vector Fields Physical meanings Relations between the vector differential operators.
University of Calicut
11
Module IV: Vector Integral Calculus (15 hours) Double and Triple integrals Their evaluation: Line, Surface and Volume integrals Greens Theorem Gauss Divergence Theorem Stokes Theorem (Proofs of these theorems are excluded) Line integrals independent of the Path.
Reference books 1. Michael D Greenberg, Advanced Engineering Mathematics (2nd Edition), Pearson Education Asia. 2. Wylie C.R and L.C. Barrent, Advanced Engineering Mathematics, McGraw Hill. 3. Kreyzig E., Advanced Engineering Mathematics, Wiley eastern. 4. Piskunov N., Differential and Integral calculus, MIR Publishers. 5. Ayres F., Matrices, Schaums Outline Series, McGraw Hill. 6. Sastry S.S., Advanced Engineering Mathematics-Vol. I and II., Prentice Hall of India. 7. Glyn James., Advanced Engineering Mathematics, 3/e, Pearson Education Asia. 8. Dr.ChandraMohan,Dr.Varegheese Philip, Engineering Mathematics I,II,III & IV , Sanguine Technical Publishers. 9. Peter V ONeil, Advanced Engineering Mathematics, Thomson India Edition.
Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
University Examination Pattern PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions 4 x 5 marks=20 marks
Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions Two questions from each module with choice to answer one question. Maximum Total Marks: 70 4 x 10 marks=40 marks
12
EN09 103: Engineering Physics

(Common for all branches) Teaching scheme 2 hours lecture per week Credits: 3
Objectives To impart the basic concepts and ideas in physics To develop scientific attitudes and enable the students to correlate the concepts of physics with the core programmes Module 1(15 hours) Interfernce of light-superposition of waves-conditions for interference-spatial coherence-temporal coherence-interference in thin films-Plane parallel films- -Colours of thin films in reflected and transmitted light- interference in wedge shaped films- Testing of optical flatness-Newtons Ringstheory and expression for the radius in reflected light.-Measurement of wavelength and refractive index. Diffraction of light-Fresnel and Fraunhoffer class of Diffraction-Fresnels halfperiod zoneFraunhofer Diffraction at a single slit-(Qualitative Analysis) -Diffraction Grating- simple theory of diffraction transmission grating- Determination of wavelength of monochromatic light using grating. Rayleighs criteria for resolution of spectral lines-Resolving power and dispersive power of grating- mathematical expressions. Crystal structurespace lattice-basis- translation vector- primitive lattice cell-unit cell-No.of lattice points per unit cell-simple cubic-bcc-fcc-latticeplanes and miller indices--spacing beween three dimensional lattice planes-cubic crystals-structure of sodium chloride Distance between adjacent atoms for NaCl crystal- Reciprocal lattice-X-ray diffraction and Braggs law use in crystal studies. Module II (15 hours) Polarisation of light-Plane polarised light-Production of polarised light- Double refraction-Optic axis and principle plane-Huyghens Explanation of double refraction in uniaxial crystals-positive and negative crystals--Nicol prism - construction and working -Quarter wave and half wave plateTheory of elliptically and circularly polarised light-Analytical analysis-production and detection of plane polarised,elliptically polarised and circularly polarised light-polaroids-Optical activityLaws of optical rotation-specific rotation-Laurents half shade polarimeter-Determination of concentration of sugar solution-Applications of polarised light. Laser-introduction--spontaneous and stimulated emission-principle of laser- properties of laserEinstein coefficients and the analysis of lasing conditions- Basic components of a laser-Different types of lasers- construction,working and applications of Ruby laser-Neodymium YAG laser- He- Ne laser- semiconductor laser-Applications of laser in medicine,industry,science and communication. Holography-basic principle-Comparison with ordinary photography-Recording and reconstruction of holograms-applications . Optical fibre--Basic structure of an optical fibre - step-index fibre and graded index fibrepropagation of light in an optical fibre-acceptance angle and acceptance cone- Numerical aperture of a step-index fibre-Numerical aperture of a graded index fibre-modes of propagation-step index monomode fibre-Multimode stepindex fibre- Graded multimode fibre-Attenuation in optic fibresfibre losses-material loss,scattering loss,absorption loss,leaky modes- dispersion in optical fibresApplications .
13
Module III (15 hours) Semi-conductor physics-energy bands in solids-classification of solids on the basis of energy band gap-Fermi level-intrinsic semi conductors- carrier (electron and hole concentration) in instrinsic semiconductors-Fermi level in intrinsic semiconductors-law of mass actionElectrical conductivity- Extrinsic semiconductors- N-type and P-type-Donor and acceptor states-Fermi level in extrinsic semiconductors. Semi-conductor devices-PN junction diode-Voltage-current characteristics of a PN junction-Static and Dynamic resistance of a diode-Zener diode-Avalanche breakdown and zener breakdownzener characteristics-voltage regulation using zener diode-construction,working and uses of tunnel diode , Light emitting diode varacter diode-Solar cell- liquid crystal display-applicationsBipolar junction transistor-Action of a transistor as an amplifier-characteristics of a npn transistor in CE Configuration-input resistance-output resistance- current amplification factor. Superconductivity-Introduction--transition temperature-Meissner effect-properties of super conductors.Types of superconductors-type 1 and type 2- AC Josephsons effect- DC Jospehsons effect- Flux quantisation-Squid-High temperature superconductors-Applications of super conductivity. Module IV (15 hours) Quantum mechanics-Introduction-origin of quantum theory-black body radiation and photo electric effect (brief ideas only)-matter waves- wave packet-uncertainty principle-(two forms)Time dependent Shrodinger equation for a free particle-Particle in force field and time dependent Schrodinger equation-Time independent schrodinger equation-Physical intrepretation of wave function-application -Particle in a Box (one dimensional) Energy eigen values and wave functions Ultrasonics-piezo electric effect-Magnetostriction effect-production of ultrasonics-properties of ultrasonics- ultrasonic diffractometer and determination of velocity of ultrasonics in a liquidApplication of ultrasonics in non destructive testing - Accoustics of building-reverberationAbsorption Coefficient- Sabines formula for reverberation time(Derivation)-Accoustic intensityloudness-decibel-phon-conditions for good acoustics(Qualitative study). Nanoscience-basic ideas of Nanoscience and nano technology-Nano clusters-carbon nanotubesproperties and applications-Future prospects and applications of Nanotechnology (Qualitative ideas) Text Books 1. Physics for Engineers-M.R.Seenivasan-New Age Publishers 1996 Edition. 2. A Text book of Engineering Physics-A.S.Vasudeva S.Chand publishers 2008 Edition 3. A Text book of Electronics-S.L.Kakani and K.C. Bhandari-New Age International(p) publishers 2000 Edition 4. Nanoscience and Technology-VS Muralidharan& A.Subramania-Ane Books Pvt.Ltd.2009 Edition Reference books. 1. Fundamentals Optics- Jenkins F.A. and White H.E. Mc Graw Hill Publication 2. Optics-Ajoy Ghatak- Tata McGraw-Hill Publishing companyLtd 3. Introduction to solid state physics- Charles Kittel-Wiley Eastern 4. Concepts of Modern Physics Arthur Beiser- Tata McGraw-Hill Publishing company Ltd 5. Lasers and non linear optics-B.B.Laud-Wiley Eastern 6. Introduction to Semi conductor materials and Devices-Tyagi M.S. Jhon wiley and Sons. 7. Nano:The essentials-T.Pradeep-Tata McGraw-Hill Publishing company Ltd. 8. Optical Fibres and Fibre Optic Communication Systems-Subir Kumar Sarkar-S.Chand Publishers 9. Engineering Physics-G.S.Raghuvanshi-Printice Hall of India 10. Text book of Optics-Brijlal and Subramanyam-S.Chand publishers 11. Modern Physics- Murukesan R-S.Chand and Co. 12. A Text book of Sound-N. Subramaniam &Brij Lal-Vikas publishing house Ltd.
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University Examination Pattern PART A: Short answer questions (one/two sentences) All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions 4 x 10 marks=40 marks Two questions from each module with choice to answer one question. Maximum Total Marks: 70 4 x 5 marks=20 marks 5 x 2 marks=10 marks
EN09 103(P) : Physics Lab

(Common for all branches) Teaching scheme 1 hour practical per week Objectives To develop scientific and experimental skills of the students To correlate the theoretical principles with application based studies. Credit: 1
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20.
Characteristics of Zener diode. Determination of band gap energy in a semi-conductor using a reverse biased p-n junction Voltage regulation using Zener diode. Static characteristics of a transistor in common emitter configuration Characteristics of photo diode Characteristics of a LED and wavelength of emitted radiation. Draw the characteristics of a solar cell. Wavelength of mercury spectral lines using diffraction grating and spectrometer. Determination of angle of a Quartz /calcite prism and hence determine the refractive indices of ordinary and extra ordinary rays in calcite or quartz prism Diameter of a thin wire or thickness of a thin paper by Air-wedge method. Wavelength of sodium light by Newtons Ring method. Radius of curvature of the lens by Boys method Specific rotation of cane sugar solution using polarimeter. To investigate the relationship between optical activity and wavelength. Wavelength of laser using Grating. Standardise the Grating using sodium light. To study the relation between the sine of the angle of diffraction and the wavelength of light. Resolving and dispersive power of Grating. To determine the angular divergence of a laser beam using He-Ne laser or diode laser. To measure the numerical aperture of an optical fibre. Wavelength and velocity of ultrasonic waves using ultrasonic diffractometer. Frequency of electrically maintained tuning fork (transverse and longitudinal modes)
15
(Minimum 12 experiments should be completed)
Reference books.
1.Practical physics with viva voice-Dr.S.L.Gupta and Dr.V.Kumar-pragati Prakashan Publishers 2.Experiments in Engineering Physics-M.N.Avadhanulu,A.A.Dani and
Internal Continuous Assessment (Maximum Marks-50) 50% - Laboratory practical and record 40% - Test 10% - Regularity in the class
End Semester Examination (Maximum Marks-50) 70% - Procedure and tabulation form, Conducting experiment, results and inference 20% - Viva voce 10% - Fair record
EN09 104: Engineering Chemistry

(Common for all branches)) Teaching scheme 2 hours lecture per week Credits: 3
Objectives To familiarise the students on application oriented themes like the chemistry of materials used in engineering discipline To focus the students on the chemistry of compounds resulting from pollution, waste generation and environmental degradation and to apply the knowledge in solving these current environmental problems effectively.
16
Module I (18 hours) Semi conductors Band theory intrinsic and extrinsic semiconductors conductivity in semiconductors Effect of temperature on conductivity - semi conductivity in non-elemental materials stoichiometric and nonstoichiometric semiconducting compounds ultrapure silicon production zone refining Introduction to Nanoscience carbon nanotubes and nanowires applications Liquid crystals classification Molecular ordering identification polymeric liquid crystals application of liquid crystals displays and thermography Water specification for various purposes- (industrial, domestic, drinking) Analysis of water Hardness, alkalinity Disadvantages of hard water determination of hardness- EDTA method softening lime soda, Ion exchange methods purification of water for domestic use Estimation of dissolved oxygen Module II (18 hours) Polymers classification Types of polymerization addition, condensation, co-polymerisation, co-ordination polymerization Mechanism cationic, anionic, free radical Polymerisation techniques Bulk, solution, suspension and emulsion Structure relation to properties Themoplastics PE, PVC, PS, PVA - Thermosetting Bakelite, UF, Silicones - Fibres Nylon 6, Nylon 66, Dacron - Natural rubber Vulcanisation - Synthetic rubber Buna S, Buna N, - Silicon rubber compounding Applications of polymers in Electrical and Electronic industry Lubricants Theories of friction Mechanism of lubrication Thick film, thin film, extreme pressure. Classification solid, liquid, semisolid properties viscosity, flash point, fire point, cloud and pour point, Aniline point, corrosion stability. Module III (12 hours) Electrochemistry single electrode potential Helmholtz double layer Nernst equation derivation types of electrodes (M/M+, M/MA/A-, M/A+, A2+, Pt/H2,H+, glass electrode) Electrochemical cells, concentration cells - salt bridge emf measurement Poggendorfs compensation method Electrochemical series applications storage cells Lead acid accumulator alkaline cells Nickel cadmium fuel cells H2/O2 fuel cell solar cells Chemical sensors. Acids and basis Lowry Bronsted and Lewis concepts. Concept of pH pH measurement using glass electrode Dissociation constants Buffer solution Henderson equation for calculation of pH Module IV (12 hours) Corrosion and its control theories of corrosion dry corrosion and wet corrosion galvanic series - corrosion of iron in acidic, neutral and basic conditions Differential aeration corrosion, stress corrosion galvanic corrosion Factors influencing corrosion. Corrosion protection self protecting corrosion products Pilling Bed worth rule- Coatings Organic (Paints and polymers) Inorganic Metallic (galvanizing, tinning, electroplating, cementation) Nonmetallic (phosphate, chromate, anodising, chemical oxide). Passivation of metals by chemical treatment protection by sacrificial anode Impressed current. Pollution Definitions classification of pollutants Effect on environment Air pollution Photochemical Smog Ozone depletion Chapman cycle of Ozone formation CFC dissociation and its reaction with Ozone Alternate refrigerants Thermal pollution Methods of control of air pollution - water pollution BOD, COD determination. Text Books 1. Jain and Jain (2007) Engineering Chemistry Dhanpat Rai Publishing Co. 2. Shashi Chawla (2006) A text Book of Engineering Chemistry Dhanpat Rai publishing Co. 3. Dr. Kochubaby Manjooran Modern Engineering Chemistry Kannantheri Publication, Kochi.
Reference Books 1. 2. 3. 4. 5. 6. B.R. Gowarikar et. al Polymer Science New Age International. S. Deswal and A. Deswal A basic course in Environmental Studies Dhanpat Rai publishing Co. A.K. De Environmental Chemistry New Age International. B.K. Sharma Electrochemistry Goel Publishing House. V. Raghavan Material Science and Engineering A First Course Prentice Hall of India Pvt. Ltd. V.S. Muraleedharan and A. Subramania Nano Science and Technology, Ane Books Pvt. Ltd., New Delhi.
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University Examination Pattern PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions Two questions from each module with choice to answer one question. Maximum Total Marks: 70 4 x 10 marks=40 marks 4 x 5 marks=20 marks
EN09 104(P): Chemistry Lab

(Common for all branches)
Teaching scheme 1 hour practical per week Credit: 1
Objectives To equip the students with the working knowledge of chemical principles, nature and transformation of materials and their applications. To develop analytical capabilities of students so that they can understand the role of chemistry in the field of Engineering and Environmental Sciences 1. Estimation of ion in Mohrs salt using standard K2 Cr2O7 2. Estimation of iron in a sample of iron ore 3. Estimation of copper in a given sample of brass
4. 5. 6. 7. 8. 9. 10. 11. 12.
Estimation of total hardness in a given sample of water using EDTA. Estimation of chloride ions in domestic water Determination of dissolved oxygen present in a given sample of water (Winklers Method) Determination of available chlorine in a sample of bleaching powder Determination of flash point and fire point of an oil using Pensky Martens flash point apparatus Determination of EMF of a cell by Poggendorfs compensation method Preparation of buffers and standardization of pH meter Estimation of iron, chromium, lead and Cadmium in water Colourimetrically Preparation of urea formaldehyde and phenol formaldehyde resin
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(Minimum 8 experiments should be completed) Reference Books A.I. Vogel A text book of Quantitative Analysis ELBS, London. Dr. Sunita Rattan Experiments in Applied Chemistry S.K. Kataria and Sons, New Delhi.
EN09 105: Engineering Mechanics

Objectives To acquaint with general method of solving engineering problems. To illustrate the application of the methods learned in Mechanics in practical engineering problems. Units: System International Module I (16 hours) Introduction to engineering mechanics - units - dimensions - vector and scalar quantities - laws of mechanics - elements of vector algebra - important vector quantities - equivalent force systems -
translation of a force to a parallel position - resultant of a force system - simplest resultant of special force systems - distributed force systems - equations of equilibrium - free body diagrams free bodies involving interior sections - general equations of equilibrium - problems of equilibrium - static indeterminacy. (Both vector and scalar formulations are to be introduced to solve problems.) Module II (16 hours) Friction laws of friction simple contact friction problems. Introduction to structural mechanics - trusses - analysis of simple trusses - method of sections method of joints. Properties of simple and composite plane areas and curves first moment and centroid theorems of Pappus-Guldinus - second moment of plane and composite areas parallel and perpendicular axis theorems polar moment of inertia of area product of inertia and principal axis (conceptual level treatment only). Moment of inertia of a rigid body and lamina (derivation of MI for cylinder, rod and sphere). Module III (14 hours) Kinematics of particles - rectilinear motion - curvilinear motion motion of a projectile tangential and normal acceleration Kinetics of particles - rectilinear motion curvilinear motion - Newtons second law DAlemberts principle motion on horizontal and inclined surfaces motion of connected bodies. Work, power and energy work-energy equation transformation and conservation of energy impulse and momentum. Module IV (14 hours) Kinematics rigid bodies - rotation of a rigid body about a fixed axis - plane motion of a rigid body instantaneous center Kinetics rigid bodies - equations of motion of a rigid body rotating about a fixed axis - rotation under the action of a constant moment - DAlemberts principle equations of motion for general plane motion - principle of work and energy.
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Text Books th 1. Shames I.H, Engineering Mechanics - Statics and Dynamics, 4 ed.,Prentice-Hall of India, New Delhi, 2001 nd 2. Hibbeler R. C. , Engineering Mechanics, Vol.I statics, Vol II Dynamics, 2 ed., Pearson Education, Delhi, 2004. th 3. Timoshenko S. and Young D. H., Engineering Mechanics, 4 ed., McGraw Hill International Edition, Singapore, 1956. Reference Books 1. Beer F.P and Johnston E.R., Vector Mechanics for Engineers - Vol.1 Statics and Vol.2 rd Dynamics, 3 ed., Tata McGraw Hill, New Delhi, 2000. th 2. Meriam J.L and Kraige L.G., Engineering Mechanics - Vol.1 Statics and Vol.2 Dynamics, 5 ed., Wiley Student Edition, Kundli, 2004 3. Rajasekharan S. and Sankarasubramanian G., Engineering Mechanics Statics and Dynamics, rd 3 ed., Vikas Publishing House, Delhi, 2005 Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
20
University Examination Pattern PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions Two questions from each module with choice to answer one question. Maximum Total Marks: 70 4 x 10 marks=40 marks 4 x 5 marks=20 marks
EN 09 106: Basics of Civil and Mechanical Engg.

(Common for all branches)) Credits: 4
Section1: Basicsof CivilEngineering

Teaching scheme 1 hour lecture per week Objective To give a basic knowledge of the topics in Civil Engineering. (In- depth treatment is not required) Module I (15 hours) Scope of Civil Engineering- Role of Civil Engineers in nation building. Brief description of Engineering properties and applications of the following construction materials (i) Lateritic stone (ii) brick (iii) cement (iv) sand (v) Rubble & Crushed stone (vi) Timber (vii) Iron & steel. (Study on laboratory tests not expected, detailed manufacturing processes of materials not expected). Stone and brick masonry construction- bonds used in general constructions- Cement mortar and Cement Concrete - Properties and applications- Reinforced Cement Concrete Fundamentals points to be observed during masonry construction and concreting. (Only brief description is expected).
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Module II (15 hours) Introduction to Surveying- brief description of the following instruments (i) chain and accessories (ii) Dumpy level (iii) Theodolite. Use of levelling instrument for determining reduced levels of various stations- Simple problems on Levelling - use of theodolite for measuring horizontal angles Simple problems on horizontal distance and plane area. (Only brief description is expected).
Building drawing- plan, section and elevation of a single room building with RCC roof (sketching in the paper/note book only is expected).
Type and functions of the following structural components of buildings (i) Foundation (ii) Wall (iii) Column (iv) Beam (v) Slab (vi) Arch & Lintels (vii) Plane Trusses. Geometric, structural, and functional features of Roads, Bridges and Dams.
Text Books 1. L.S.Jayagopal and R. Rudramoorthy-Basic Civil and Mechanical EngineeringVikas Publishing house Pvt Ltd, New Delhi New-110014. Reference Books 1. Rangwala. S. Engineering Materials, Charator book stall, Anand 2. Arora. K.R. Surveying Vol I and Vol II, Standard Book house, 3. Punmia. B.C- Building Construction, Laxmi Publications 4. Rajput. R.K.Engineering Materials, S. Chand and Company 5. Balagopal. T.S. Prabhu et.al Building Drawing and Detailing, Spades.
University Examination Pattern for Section 1 Note: Section 1 and Section 2 are to be answered in separate answer books PART A: Short answer questions (one/two sentences) 2 x 2 marks=4 marks 1 x 1 mark = 1 mark All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. 2 x 5 marks=10 marks
PART B: Analytical/Problem solving questions Candidates have to answer two questions out of three. There should be at least one question from each module and not more than two questions from any module.
PART C: Descriptive/Analytical/Problem solving questions 2 x 10 marks=20 marks Two questions from each module with choice to answer one question.
22 Section2: BasicsofMechanical Engineering

Teaching scheme 1 hour lecture and 1 hour tutorial per week Objectives To impart the basics of thermodynamics, heat engines, thermal power plants, hydraulic machines and power plants, renewable energy power plants etc To impart the basics of Power transmission elements and basic manufacturing processes. Only preliminary understanding of the subject is to be imparted, as this is a basic course. Module I (Basic Thermodynamics and applications) (16 hours) Basic Thermodynamics- Concept of temperature, Zeroth law, heat and work, First law, Internal Energy concept, Second law (Statement and explanation only) Heat engine, refrigerator and heat pump _ Concepts of entropy - Thermodynamic cycles -Carnot cycle, Otto cycle, Diesel cycle, Brayton cycle. Internal Combustion Engines Classification - SI and CI engines, Two stroke and Four stroke engines, Carburetted and MPFI engines, CRDI engines. Working principles only. Power plants layout and working of Gas turbine Power plants, Steam power plants, Diesel power plants and Nuclear power plants. Vapour compression and Air refrigeration systems Concept of Ton of Refrigeration and COP, simple cycle and schematic diagram only. Brayton cycle- derivation of efficiency of the cycle simple problems Renewable and non renewable sources of energy Fuels and their properties coal, LSHS, FO, LNG , HSD, Biodiesel and biogas layout and working principles of solar, wind, tidal, OTEC, Geothermal, power plants. Module II (Hydraulic machines and Manufacturing systems) (14 hours) Hydel power plants and pumps.- Hydraulic Turbines - Classification, construction, working and applications. Hydraulic pumps - Classification, Reciprocating and centrifugal pumps, Priming, multistage pumps, pumps like vane, gear and jet pumps (working principle and applications only). Basic Power transmission systems- Belt and chain drives, expressions for power transmitted, belt tension, & coefficient of friction relationshipsgears and gear trains - Rack & pinion, Slider crank mechanism, eccentric mechanism (basics only) Basic manufacturing processes- Casting (Sand and die casting processes), Forging (open and closed die forging & net shape forging), Rolling (2 and 3 roll process), Extrusion (Direct, indirect and hydrostatic extrusion), Welding (SMAW & Oxy fuel welding) Reference Books 1. A textbook on Internal Combustion Engines Mathur and Sharma, Dhanpat Rai & Sons 2. Elements of Mechanical Engineering Roy and Choudhary 3. Power plant engineering P.K. Nag, Tata Mc Graw Hill 4. Basic Mechanical Engineering Benjamin Internal Continuous Assessment (Maximum Marks-15) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
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University Examination Pattern for Section 2 Note: (Section 1 and Section 2 are to be answered in separate answer books) PART A: Short answer questions (one/two sentences) 2 x 2 marks=4 marks 1 x 1 mark = 1 mark All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. 2 x 5 marks=10 marks
PART C: Descriptive/Analytical/Problem solving questions 2 x 10 marks=20 marks Two questions from each module with choice to answer one question. Maximum Total Marks: 35
EN09 107: Basics of Electrical, Electronics & Communication Engineering

(Common for all branches) Teaching Scheme 2 hours lecture and 1 hour tutorial per week Credits: 4
Section1:BasicsofElectricalEngineering
Objective To provide the basic concepts and an overview of Electrical Engineering. Module I (15 hours) Kirchoffs laws solution of series-parallel circuits with DC excitation. (2Hrs) Magnetic circuits MMF, flux, reluctance comparison of magnetic and electric circuits. (3 Hrs) Faradays laws Lenzs law statically and dynamically induced EMF self and mutual inductance coefficient of coupling. (3 Hrs) Single phase AC circuits generation of sinusoidal EMF cycle, frequency, time period average and RMS values form factor and peak factor of sine wave only analysis of simple R,L,C circuits reactance and impedance active, reactive and apparent power power factor. (5Hrs) 3-phase circuits generation of 3-phase AC voltage Star and Delta connection voltage & current relationships in star and delta (balanced only). (2 Hrs) Module II (15 Hours) (Basic Concepts only) Single phase transformer construction principle of operation EMF equation transformation ratio ideal transformer only. (3 Hrs) DC motor and generator constructional details EMF equation of dc generator - shunt, series and compound (schematics only) applications of dc motors. (3 Hrs)
3-phase induction motor squirrel cage and wound rotor type constructional details rotating magnetic field (concepts only) - principle of operation slip applications. (3 Hrs) 3-phase synchronous generators constructional details salient-pole and cylindrical rotor type principle of operation. (3 Hrs) Basic structure of ac power system (1Hr) Electrical estimation of small residential building (quantity of materials only) (2Hrs) Text Books 1. Edward Hughes, Electrical Technology, Pearson Education 2. Vincent Del Toro, Electrical Engineering Fundamentals , Pearson Education Reference Books 1. Kothari & Nagrath, Theory & problems of Basic Electrical Engineering, Tata McGraw Hill 2. Ashfaq Husain, Fundamentals of Electrical Engineering, Dhanpat Rai & Co. 3. J.B. Gupta, A course in electrical installation, estimation & costing, S.K. Kataria & Sons
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25 Section2: BasicsofElectronicsandCommunicationEngineering
Objectives To impart knowledge about basic electronic and digital systems To give basic ideas about various communication systems (Only system level block diagram approach, no analysis required) Module I (14 hours) Amplifiers: Principle of electronic amplifiers Block diagram representation Classification Significance of input impedance, output impedance, output power, power gain, voltage gain and frequency response noise in amplifiers cascaded amplifiers concept of differential amplifiers and operational amplifiers open loop and closed loop systems effect of negative feedback concept of oscillators.(7 Hours) Digital Systems : Logic gates logic states Boolean algebra algebraic logic minimisation generating logic diagram from Boolean expression introduction to TTL and CMOS logic programmable logic devices . (4 Hours) Measurements and Data Acquisition Systems : Working and block diagram of CRO sensors actuators principle of digital voltmeter concept of multiplexing principle of ADC and DAC .. (3 Hours) Module II (16 hours) Radio Communication : Modulation - Principle Of AM & FM block diagrams of transmitters wave forms band width principle of AM & FM demodulation - comparison of AM &FM principle of super heterodyne receiver block diagram. (4 Hours) Radar and Navigation : principle of Radar Radar equation [ Derivation not required ] block schematics of pulsed Radar and continuous wave Radar applications of Radar introduction to navigational aids. (4 Hours) Communicational Systems : principle of microwave communication frequency band repeaters block diagrams principle of satellite communication systems transponder block diagram of optical communicational systems principle of light transmission through fibre advantages of optical communication basic principles of cellular communications concepts of cells frequency reuse handoff roaming principle of GSM , CDMA, GPRS technologies . (8 Hours) Text Books 1. Neil Storey, Electronics; A Systems Approach Pearson Education, 2nd Ed., New Delhi 2. Santhiram Kal.Basic Electronics-Devices, Circuits & IT fundamentals, PHI, New Delhi 3. Louis E Frenzel,Principles of Electronic Communication systems, Tata McGraw Hill, New Delhi 4. William Stallimgs.Wireless Communications & Networks, Pearson Education, New Delhi 5. David A Bell.Electronic Instrumentation & Measurements, PHI, New Delhi Internal Continuous Assessment (Maximum Marks-15) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
B.Tech. Degree Programme Syllabi - Combined First & Second Semesters
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EN09 108: Engineering Graphics

(Common for all branches)
Teaching scheme 3 hours drawing per week

Objectives
Credits: 3
By going through the contents student will be able to: Understand systems of drawing. Produce orthographic drawing of points, lines and solids. Produce isometric views of any object. Develop skill to produce perspective views of any object.
Develop skill to convert the pictorial views of simple engineering objects into orthographic views. Module I (9 Hours; 1 Drawing Exercise) Drawing instruments and their use - Different types of lines - Lettering and dimensioning Scales - Familiarization with current Indian Standard Code of practice for general engineering drawing Construction of Conic sections - Construction of Cycloid, Involutes and Helix (For internal work assessment only, not for University Examination) Module-II (24 Hours; 4 Drawing exercises) a) Introduction to projections - Systems of projections - Vertical, Horizontal and Profile planes - Principles of first and third angle projections - Projections of points in different quadrants - Orthographic projections of straight lines parallel to both reference planes Perpendicular to one of the reference planes - Inclined to one and parallel to other reference plane - Inclined to both the reference planes and occupied in one quadrant Traces of lines - True length and inclination of a line with reference planes - Line occupied
in more than one quadrant - Line inclined to the two reference planes but parallel to the profile plane. b) Projections of plane lamina of geometrical shapes - Plane lamina parallel to one of the reference planes - Inclined to one and perpendicular to the other reference plane - Inclined to both the reference planes - Inclined to the two reference planes but perpendicular to the profile plane. Module- III (18 Hours; 3 Drawing exercises) a) Projections of Polyhedra, Solids of revolution and Frustums - Projections of solids with axis parallel to one and inclined to the other reference plane - Axis inclined to both the reference planes - Projections of solids on auxiliary planes (Solids to be drawn: Cube, Prisms, Pyramids, Tetrahedron, Cone and Cylinder) b) Sections of solids - Sections by cutting planes parallel to the reference planes - Cutting plane inclined to one and perpendicular to other reference plane - True shape of the section by projecting on auxiliary plane (Solids to be drawn: Cube, Prisms, Pyramids, Tetrahedron, Cone and Cylinder) Module- IV (15 Hours; 3 Drawing exercises) a) Intersection of surfaces - Methods of determining the curve of intersection - Line method Cutting plane method - Axes of the solids perpendicular to each other and one of them is perpendicular to vertical plane and other is perpendicular to horizontal plane - Intersection of two prisms - Intersection of two cylinders Intersection of cylinder and cone. b) Development of surfaces of solids - Method of parallel line and radial line developments Development of Polyhedra, Cylinder, Cone and sectioned solids - Development of solids having hole or cut - Development of Elbow and T-joint. Module- V (15 Hours; 3 Drawing exercises) a) Introduction to isometric projection - Isometric scale - Isometric views - Isometric projections of Prisms, Pyramids, Cylinder, Cone, Spheres, sectioned solids and combination of them. b) Introduction to perspective projections - Classification of perspective views - Visual ray and vanishing point method of drawing perspective projection - Perspective views of plane figures such as polygons and circles - Perspective views of solids like Prisms, Pyramids and Cube. c) Introduction to multiview projection of objects - The principle of the six orthographic views Conversion of pictorial views of simple engineering objects into orthographic views. Module- VI (9 Hours; 2 Drawing exercises) a) Introduction to Computer Aided Drafting (CAD) - Preparation of engineering drawings by using any software capable of drafting and modeling - Creation of simple figures like polygon and general multiline figures - Drawing of front view and top view of solid like Prism, Pyramid and Cylinder and dimensioning - Drawing of front view and top view of objects from pictorial view. b) Conventional representation of threaded fasteners - Drawing of nuts, bolts, washers and screws - Locking arrangements of nuts - Bolted and screwed joints - Foundation bolts. (For internal work assessment only, not for University Examination) NOTE: All drawing exercises mentioned above are for class work. Additional exercises where ever necessary may be given as home assignments
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Text Books 1. John.K.C, Engineering graphics, Jet Publications, Thrissur 2. P.I.Varghese, Engineering Graphics, VIP Publications, Thrissur 3. Bhatt.N.D, Elementary Engineering Drawing, Charotar Publishing House, Delhi 4. K.N.Anilkumar, Engineering Graphics, Adhuth Narayanan Publishers, Kottayam
Reference Books.
Internal Continuous Assessment (Maximum Marks-30) 60% - Drawing exercises (Best 12 sheets) 30% - Tests (minimum 2) 10% - Regularity in the class
University Examination Pattern No question from modules I and VI Answer THREE questions from Part A and any TWO questions from Part B. All questions carry equal marks PART A Q 1 Two questions (a) and (b) of 14 marks each from module 3 x 14 marks= II, one from module II (a) and one from module II(b), with 42 marks choice to answer any one. Q II Two questions (a) and (b) of 14 marks each from module III, one from module III(a) and one from module III(b), with choice to answer any one. Q III Two questions (a) and (b) of 14 marks each from module IV, one from module IV(a) and one from module IV(b), with choice to answer any one. Q IV 3 Questions (a), (b) and (c) of 14 marks each from module 2 x 14 marks = V, one from module V(a), one from module V(b) and one 28 marks from module V(c), with choice to answer any two. Maximum Total Marks=70
PART B
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EN09 109(P): Computer Programming in C

(Common for all branches) Teaching scheme 1 hour lecture and 1 hour practical per week Credits: 3
Objectives To impart the basic concepts of computer and information technology To develop skill in problem solving concepts through learning C programming in practical approach. Module I (7 hours) Introduction to Computers: CPU, Memory, input-output devices, secondary storage devices, Processor Concepts - Evolution and comparative study of processors. Machine language, assembly language, and high level language. Inside a PC, Latest trends and technologies of storage, memory, processor, printing etc. Concept of Program and data, System software - BIOS, Operating SystemDefinition-Functions-Windows, and Linux. Compilers and assemblers. Application software. Definition and scope of IT, Computer networks, LAN, WiFi, Internet Services. Module II (6 hours) Basic elements of C: Flow chart and algorithm Development of algorithms for simple problems. Structure of C program Operators and expressions Procedure and order of evaluation Input and Output functions. While, do-while and for statements, if, if-else, switch, break, continue, goto, and labels. Programming examples. Module III (7 hours) Functions and Program structures: Functions declaring, defining, and accessing functions parameter passing methods Recursion Storage classes Extern, auto, register and static. Library functions. Header files C pre-processor. Example programs. Arrays: Defining and processing arrays passing arrays to functions two dimensional and multidimensional arrays application of arrays. Example programs. Module IV (10 hours) Pointer: Concepts, declaration, initialization of pointer variables Pointers and functions pointers and arrays Pointers and structures Command line arguments Dynamic memory allocations. Example programs. Structures, unions, and file handling: Structures declaration, definition and initialization of
structures Nested structures Arrays of structures Structures and function. Union typedef. Concept of a file File pointer File operations. Basic concepts of linked lists. Example programs. Lab Exercises 1. Lab Practice Familiarization of OS- DOS, Windows and Linux Simple OS commands Creation of folders/directories, copying and deleting files etc. Simple shell programming (3 Hrs) 2. Lab praise- identifying the hardware components inside a computer (2 hrs) 3. Lab Practice/Demo - Editor, compiler, linker, loader (with a simple C program) (3 Hrs) 4. Lab Practice- Common application softwares DTP & Office suite, Presentation slides, pdf and ps reader (5 Hrs)
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5. Usage of INTERNET for academic purposes, ftp, torrent demo (2 hours) 6. Programming exercises in C covering the following topics (15 hours) (a) Functions (b) Arrays (c) Pointers (d) Structures and unions (e) File handling
Text Books nd 1. B.Gottfried, Programming with C, 2 ed, Tata McGraw Hill, New Delhi, 2006 2. P. Norton, Peter Nortons Introduction to Computers, 6th ed., Tata McGraw Hill, New Delhi, 2004. 3. B. W. Kernighan, and D. M. Ritchie, The C Programming Language, Prentice Hall of India, New Delhi, 1988 4. E. Balaguruswamy, Programming in ANSI C, 3 rd ed., Tata McGraw Hill, New Delhi, 2004 Reference Books nd 1. K. N. King. C Programming: A Modern Approach, 2 ed., W. W. Norton & Company, 2008 2. P. Norton, Peter Nortons Computing Fundamentals, 6th ed., Tata McGraw Hill, New Delhi, 2004. 3. S .Kochan , Programming in C, CBS publishers & distributors 4. M. Meyer, R. Baber, B. Pfaffenberger. Computers in Your Future, 3rd ed., Pearson Education India Internal Continuous Assessment (Maximum Marks 50) 40% 20% 30% 10% Laboratory practical and record Test(s) Assignments such as home work, term-project, programming exercises, etc. Regularity in the class
End Semester Examination Pattern (Maximum Marks 50) The examination shall be conducted in two sections, theory section of 1 hour duration and practical section of 2 hours duration. Section 1 (Theory) This will be a common test; question paper shall be set jointly by external and internal examiners. PART A: Short answer questions 5 x 1 marks=5 marks All questions are compulsory. There should be at least one question from each module. PART B: Descriptive/Analytical/Problem solving questions Two questions from each module with choice to answer one question. Total Marks: 25 Section 2 (Practical) (Total Marks: 25)
B. 70% - Algorithm/Procedure, Writing and executing C-program, Results/Inference
4 x 5 marks=20 marks
20% - Viva voce 10% - Record
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EN09 110(P): Mechanical Workshops

(Common for all branches) Teaching scheme 2 hours practical per week Credits: 2
Objectives
To inculcate engineering aptitude, confidence and experience towards technical skills To train the students mentally and physically for industries To impart knowledge and technical skills on basic manufacturing methods
A) Carpentry: study of tools and joints plaining, chiselling, marking and sawing practice, Different joints B) Fitting: study of tools, chipping, filing, cutting, drilling, tapping, male and female joints, stepped joints C) Smithy: study of tools, forging of square prism, hexagonal bolt D) Foundry: study of tools, sand preparation, moulding practice E) Sheet Metal work: study of tools, selection of different gauge sheets, types of joints, trays and containers F) Plumbing Practice: study of tools, study of pipe fittings, pipe joints, cutting, threading and laying practice.
Note : For end-semester examination, the student shall be examined in any one of the first five trades (A-E)
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EN09 111(P) Electrical and Civil Work shops

(Common for all branches) Teaching Scheme 2 hours practical per week Credits: 2
Section1:ElectricalEngineeringWorkshop
Objective To impart a basic knowledge of electrical circuits, machines and power systems.
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.
Familiarization of various types of Service mains Wiring installations Accessories and house hold electrical appliances. Methods of earthling- Measurement of earth resistance- Testing of electrical installationsPrecautions against and cure from electric shock Practice of making different joints( Britannia, Married and T- Joints) on copper/ aluminium bare conductors. Wiring practice of a circuit to control two lamps by two SPST switches. Wiring practice of a circuit to control one lamp by two SPDT switches. Wiring practice of a circuit to control one fluorescent lamp and one three pin plug socket. Wiring practice of a main switch board consisting of ICDP switch, DB, MCBs and ELCBs. Familiarization of various parts of electrical motors and wiring of three phase and single phase motor with starter. Familiarization of energy meter and measurement of energy consumption by a single phase load. Familiarization of various electrical and electronic components such as transformers, resistors, AF and RF chokes, capacitors, transistors, diodes, ICs and PCB. Assembling and soldering practice of single phase full wave bridge rectifier circuit with i) capacitor circuit ii) regulator IC
33 Section2: CivilEngineeringWorkshop
Objectives To provide experience on plotting, measuring/determining horizontal distances, level differences between stations and horizontal angles.
To provide experience on setting out for small buildings, masonry construction, plumbing work and model making.
1. Chain Surveying - Study of chain and accessories, Plotting one side of a building/ Five or six points in the field using chain and cross-staff 2. Compass surveying (Study of compass, Plotting one side of a building/Five or six points in the field using compass 3. Levelling - Study of levelling instruments, Determination of reduced levels of five or six points in the field. 4. Theodolite - Study of Theodolite, Measuring horizontal angles 5. Setting out practice 6. Brick Masonry 7. Plumbing - Demonstration of plumbing fixtures-Exercise in joints 8. Model making of simple solids
Internal Continuous Assessment (Maximum Marks-25) 50% - Laboratory practical and record 40% - Test 10% - Regularity in the class End Semester Examination (Maximum Marks-25) 70% - Procedure, conducting experiment, results, tabulation, and inference 20% - Viva voce 10% - Fair record
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EN09 301: ENGINEERING MATHEMATICS III

(COMMON FOR ALL BRANCHES) Teaching scheme 3 hours lecture and 1 hour tutorial per week Credits: 4
Objective This course provides a quick overview of the concepts and results in complex analysis that may be useful in engineering. Also it gives an introduction to linear algebra and Fourier transform which are wealth of ideas and results with wide area of application. Module I: Functions of a Complex Variable (13 hours) Functions of a Complex Variable Limit Continuity Derivative of a Complex function Analytic functions Cauchy-Riemann Equations Laplace equation Harmonic Functions Conformal Mapping Examples: Zn, sinz, cosz, sinhz, coshz, (z+1/Z ) Mobius Transformation. Module II: Functions of a Complex Variable (14 hours) Definition of Line integral in the complex plane Cauchys integral theorem (Proof of existence of indefinite integral to be omitted) Independence of path Cauchys integral formula Derivatives of analytic functions (Proof not required) Taylor series Laurent series Singularities and Zeros Residues Residue Integration method Residues and Residue theorem Evaluation of real integrals. Module III: Linear Algebra (13 hours) - Proofs not required Vector spaces Definition, Examples Subspaces Linear Span Linear Independence Linear Dependence Basis Dimension Ordered Basis Coordinate Vectors Transition Matrix Orthogonal and Orthonormal Sets Orthogonal and Orthonormal Basis GramSchmidt orthogonolisation process Inner product spaces Examples. Module IV: Fourier Transforms (14 hours) Fourier Integral theorem (Proof not required) Fourier Sine and Cosine integral representations Fourier Transforms Fourier Sine and Cosine Transforms Properties of Fourier Transforms. Text Books Module I: Erwin Kreysig, Advanced Engineering Mathematics, 8e, John Wiley and Sons, Inc. Sections: 12.3, 12.4, 12.5, 12.6, 12.7, 12.9 Module II: Erwin Kreysig, Advanced Engineering Mathematics, 8e, John Wiley and Sons, Inc. Sections: 13.1, 13.2, 13.3, 13.4, 14.4, 15.1, 15.2, 15.3, 15.4 Module III: Bernaed Kolman, David R Hill, Introductory Linear Algebra, An Applied First Course, Pearson Education. Sections: 6.1, 6.2, 6.3, 6.4, 6.7, 6.8, Appendix.B.1 Module IV: Wylie C.R and L.C. Barrett, Advanced Engineering Mathematics, McGraw Hill. Sections: 9.1, 9.3, 9.5
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University Examination Pattern PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions 4 x 5 marks=20 marks Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions 4 x 10 marks=40 marks Two questions from each module with choice to answer one question. Maximum Total Marks: 70
Reeference books 1. H S Kasana, Complex Variables, Theory and Applications, 2e, Prentice Hall of India. 2. John M Howie, Complex Analysis, Springer International Edition. 3. Shahnaz bathul, Text book of Engineering Mathematics, Special functions and Complex
Variables, Prentice Hall of India.
4. Gerald Dennis Mahan, Applied mathematics, Springer International Edition. 5. David Towers, Guide to Linear Algebra, MacMillan Mathematical Guides. 6. Howard Anton, Chris Rorres, Elementary Linear Algebra, Applications Version, 9e, John Wiley
and Sons.
7. Anthony Croft, Robert Davison, Martin Hargreaves, Engineering Mathematics, 3e, Pearson
Education.
8. H Parthasarathy, Engineering Mathematics, A Project & Problem based approach, Ane Books
India. 9. B V Ramana, Higher Engineering Mathematics, McGrawHill. 10. Sarveswara Rao Koneru, Engineering Mathematics, Universities Press. 11. J K Sharma, Business Mathematics, Theory and Applications, Ane Books India. 12. John bird, Higher Engineering Mathematics, Elsevier, Newnes. 13. M Chandra Mohan, Vargheese Philip, Engineering Mathematics-Vol. I, II, III & IV., Sanguine Technical Publishers. 14. N Bali, M Goyal, C Watkins, Advanced Engineering Mathematics, A Computer Approach, 7e, Infinity Science Press, Fire Wall Media. 15. V R Lakshmy Gorty, Advanced Engineering Mathematics-Vol. I, II., Ane Books India. 16. Sastry S.S., Advanced Engineering Mathematics-Vol. I and II., Prentice Hall of India. 17. Lary C Andrews, Bhimsen K Shivamoggi, Integral Transforms for Engineers, Prentice Hall of
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EN09 302: HUMANITIES AND COMMUNICATION SKILLS

(COMMON FOR ALL BRANCHES) Teaching scheme 2 hours lecture and 1 hour tutorial per week Objectives To identify the most critical issues that confronted particular periods and locations in history To identify stages in the development of science and technology To understand the purpose and process of communication To produce documents reflecting different types of communication such as technical descriptions, proposals ,and reports To develop a positive attitude and self-confidence in the workplace and To develop appropriate social and business ethics. Maximum Total Marks: 70 Credits: 3
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Module I (14 hours) Humanities, Science and Technology: Importance of humanities to technology, education and society- Impact of science and technology on the development of modern civilization. Contributions of ancient civilization: Chinese, Indian, Egyptian and Greek. Cultural, Industrial, Transportation and Communication revolutions. Advances in modern India: Achievements in information, communication and space technologies. Module II (16 hours) Concept of communication: The speaker/writer and the listener/reader, medium of communication, barriers to communication, accuracy, brevity, clarity and appropriateness Reading comprehension: Reading at various speeds, different kinds of text for different purposes, reading between lines. Listening comprehension: Comprehending material delivered at fast speed and spoken material, intelligent listening in interviews Speaking: Achieving desired clarity and fluency, manipulating paralinguistic features of speaking, task oriented, interpersonal, informal and semi formal speaking, making a short classroom presentation. Group discussion: Use of persuasive strategies, being polite and firm, handling questions and taking in criticisms on self, turn-taking strategies and effective intervention, use of body language. Module III (16 hours) Written Communication : Note making and taking, summarizing, notes and memos, developing notes into text, organization of ideas, cohesion and coherence, paragraph writing, ordering information in space and time, description and argument, comparison and contrast, narrating events chronologically. Writing a rough draft, editing, proof reading, final draft and styling text. Technical report writing: Synopsis writing, formats for reports. Introductory report, Progress report, Incident report, Feasibility report, Marketing report, Field report and Laboratory test report Project report: Reference work, General objective, specific objective, introduction, body, illustrations using graphs, tables, charts, diagrams and flow charts. Conclusion and references Preparation of leaflets, brochure and C.V.
Module IV (14 hours) Human relations and Professional ethics: Art of dealing with people, empathy and sympathy, hearing and listening. Tension and stress, Methods to handle stress Responsibilities and rights of engineers- collegiality and loyalty Respect for authority Confidentiality conflicts of interest Professional rights, Rights of information, Social responsibility. Senses of ethics variety of moral issues Moral dilemma Moral autonomy Attributes of an ethical personality right action self interest
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PART1. Meenakshi Raman and (one/two sentences) Technical Communication- Principles and A: Short answer questions Sangeeta Sharma, 5 x 2 marks=10 marks All questions are compulsory. There Practice Oxford University press, 2006 should be at least one question from and module and not more than two questions 2. Jayashree Suresh eachB S Raghavan, Professional Ethics, S Chand and Company Ltd, 2005 from any module. 3. Subrayappa, History of Science in India, National Academy of Science, India 4. R C Bhatia, Business Communication, Ane Books Pvt. Ltd, 2009 PART5. Sunita Mishra and C Muralikrishna, Communicatin Skils for Engineers, marks=20 marks B: Analytical/Problem solving questions 4 x 5 Pearson Education, 2007. Candidates have to answer four questions out of six. There 6. Jovan van Emden and Lucinda Becker, Effective Communication for Arts and Humanities should be at least one question from each module and not more Students, Palgrave macmillam, 2009 than two questions from any module. 7. W C Dampier, History of Science, Cambridge University Press 8. Vesilind, Engineering, Ethics and the Environment, Cambridge University Press PART9. Larson E, History of Inventions, Thompson Press India Ltd. C: Descriptive/Analytical/Problem solving questions 4 x 10 marks=40 marks 10. Bernal J.D, Science in History, Penguin Books Ltd to answer one Two questions from each module with choice 11. Encyclopedia Britannica, History of Science, History of Technology question. 12. Brownoski J, Science and Human Values, Harper and Row Maximum Total Marks: 70 Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
Reference Books
University Examination Pattern PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. Analytical/Problem solving questions 4 x 5 marks=20 marks Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module.
PART B:
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BT09 303 FLUID FLOW OPERATIONS

Teaching Scheme: 4 hrs lecture and 1 hr tutorial per week Objectives:
To impart the basic concepts of fluid statics and dynamics To study the basic equations of flow To study about the metering and pumping of fluids To study about the flow of fluids through packed beds and fluidized beds
Credits: 5
Module 1 Introduction to fluid mechanics. Definition of fluid. Physical properties of fluids density, specific weight, specific gravity, compressibility, surface tension, vapour pressure and viscosity. . Absolute and kinematic viscosity. . Units and dimensions of the properties. Variation of properties with temperature and pressure. Rheology of fluids. Classification of fluids. Fluid statics and applications Pascals law. Hydrostatic equilibrium in gravity and centrifugal fields. Barometric equation. Principle of manometers. Different types of manometers. Principles of continuous gravity and centrifugal decanters. Introduction to fluid flow phenomenon. Reynolds experiment. Reynolds number. Classification of flow. Module II Basic equation of fluid flow. Equation of continuity. Equation of motion. Euler equation. Bernoulli equation. Momentum equation. Kinetic energy and fluid friction correction factors. Laminar flow of incompressible fluids in pipes and conduits. Shear stress and velocity distribution in circular channels. The friction factor, Hagen-Poiseuille equation. Darcy and Weisbach equation. Concept of equivalent diameter. Friction factor chart, Friction losses from change in velocity and direction and loss due to pipe fittings. Turbulent flow, universal velocity distribution equations, friction factor- Reynolds number relationship, Nikuradse- Carman Equation, average velocity, Blassius equation ( derivation not required), Prandtl law. Module- III Transportation and metering of fluids. Pipes and pipe standards, tubings, pipe joints, flange, expansion joints, valves, automatic control valves. Different types of pumps. Description and comparison. Detailed study of centrifugal pumps. Velocity diagrams, Developed head. Volumetric flow rate relation. Various losses. characteristic curves, NPSH. Cavitation, pump priming. Description of rotary pumps, reciprocating pumps, jet pumps, air lift, and diaphragm pumps. Comparison and application of venturimeter, orifice meter, pitot tube, rotameter, weirs and notches.
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Module IV Flow past immersed bodies: Drag coefficient, flow through packed bed. Ergun equation. Kozney- Carman equation. Blake Plummer equation. Design of packed beds. Motion of particles through fluids. Motion from gravitational and centrifugal fields. Terminal settling velocity. Stokes law. Intermediate law-Newton law. Free and hindered settling Fluidization. The phenomenon of Fluidization, Minimum fluidizing velocity, Advantages and disadvantages of fluidized beds. Industrial applications. Text /References:
1. Mccabe and Smith : Unit operations in chemical Engg McGraw - Hill. 2. Christie J Geankoplis Transport process and Unit Operations Prentice Hall of India. 3. Streeter Fluid Mechanics. McGraw Hill 4.
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BT 09 304 MICROBIOLOGY
Teaching Scheme: 3 hrs lecture and 1 hr tutorial per week Objectives: Credits: 4
To develop knowledge of the nature and characteristics of microorganisms To evaluate the role of microbes in public health and various industries
Module 1 History and development of microbiology. Microbial diversity. Principles of microbial taxonomy. Morphology ultra structure and reproduction of bacteria, actinomycetes, blue green algae, yeasts, fungi, algae and viruses. Microscopy-Bright field microscopy fluorescence microscopy, phase contrast microscopy. Electron microscopy. Theory of staining microorganisms. Simple staining. Differential staining- Gram staining, Acid fast staining, spore staining. Special staining techniques- Flagella staining. negative staining. Sterilization-Control of microorganisms by physical and chemical agents. Module II Nutritional requirements of microorganisms, Nutritional types of bacteria - Formulation of growth medium and different types of media- Synthetic media, complex media Selective media, differential media, enrichment media, enriched media. Pure culture techniques- Spread plate, Pour plate and streak plate, preservation and maintenance of cultures. Module III Microbial growth growth curve, generation time. Batch culture, Fed batch culture and continuous culture, Synchronous culture- techniques adopted to generate synchronous culture, Measurement of microbial growth, Enumeration techniques- cell numbers and cell mass. Influence of environmental factors on growth Module IV Microbiological analysis of water- Test for coliforms, Microbial flora of soil. Interactions among soil microorganisms - Mutualism, commensalisms, predation, parasitism, amensalism, competition, symbiosis, Use of micro organisms as biofertilizer and bioinsecticide Microbial spoilage of foods and preservation of foods-Physical and chemical methods, Food borne diseases and intoxications Text/Reference Books 1. M.J.Pelczar Et Al:Microbiology, Tata Mcgraw Hill 2. Prescott Et Al:Microbiology, Mcgraw Hill, USA 3. Tauro, Kapoor &Yadav; An introduction to microbiology, Wiley Eastern Ltd.
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BT 09 305 BIOCHEMISTRY I
Teaching Scheme: 3 Hrs lecture and 1 hr tutorial Credits: 4
To understand the fundamental aspects of life To attain knowledge of the elemental composition of biomolecules
Module 1 Introduction to biochemistry. A historical perspective. General features of biomolecules. Carbohydrates: structure and properties of monosaccharides, oligosaccharides and polysaccharides, Ring structure and mutarotation. Homo and heteropolysaccharides. Mucopolysaccharides. Sialic acids. Bacterial cell wall polysaccharides. Glycoproteins, membrane glycoproteins and their biological functions. Blood group substances. Module II Aminoacids and proteins: structure, nomenclature and properties of aminoacids. General reactions of aminoacids. Peptide bond. Classification of proteins, Basic understanding of primary, secondary, tertiary and quaternary structure of proteins. Denaturation and renaturation. Enzymes: Nomenclature and classification of enzymes. Activation energy and transition state enzyme activity, active sites, theories of enzyme specificity. Role of effectors and cofactors.
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Module III
Lipids - classification and structure,essential fatty acids- glycerides,hydrolysis of fats,structure and properties of phospholipids and glycolipids. Prostaglandins- structure, biological properties. Cholesterol- structure and biological importance.
Module IV Nucleic acids: structure and properties of Purine and pyrimidine bases. Nucleosides and nucleotides. Structure of nucleic acids. Base pairing role. Structure and functions of DNA and RNA Double helical model of DNA structure. A, B and Z DNA.Vitamins: Chemistry and biological functions of fat soluble Vitamin A, Vitamin D, Vitamin K and Vitamin E. Water soluble Vitamins: B complex and Vitamin c. Biological functions. Text books / References: 1. A.L. Lehninger: Principles of Biochemistry CBS publications. 2. E.L.Smith, R.L.Hill et al: Principles of Biochemistry, Vol I, MC Graw Hill Book Company. 3. Lubert Stryer: Biochemistry, W.H.Frceman co 4. Donald Voet, Judith.G.Voet: Biochemistry, Wiley and sons.
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BT 09 306 BIOPROCESS CALCULATIONS.

Teaching Scheme: 3 Hrs lecture and 1 hr tutorial per week Credits: 4
To study the laws regarding gas ,liquid and vapour To develop understanding about material balance and energy balances To study the stoichiometry and thermodynamics of microbial growth and product formation
Module 1 Overview of process industry and bioprocess industry. Definition of unit operations and unit processes. Units and dimensions. SI Units. Fundamental concepts of stoichiometry like mole concept, mass fraction, mole fraction, volume fraction, average molecular weight. Concentration of liquids and solutions. Fundamental laws for gas, vapour, and liquid , vapour pressure of pure liquids, effect of temperature on vapour pressure, Classius Claperon equation, Antoine equation. Ideal gas law, Dalton's law, Amagats law. Real gas properties, Van der Waals equation, critical state, reduced variables, compressibility factor, vapour liquid equilibria, Raoults law, and Henrys law. Humidity and saturation. Wet bulb and dry bulb temp. humidity chart Module -II Material balances. Law of conservation of mass. Material balance without chemical reactions. Total and component balances, concept of steady state, batch and continuous process. Tie element basis for calculations. Recycling and bypassing operations. Material balance with chemical reactions. Concept of excess reactant, limiting reactant, conversion, yield, degree of completion. Module-III Energy balance. Law of conservation of energy. Components of energy balance equation. Heat and work. Internal energy. Concept of Enthalpy and heat capacity. Enthalpy changes. Heat effects accompanying chemical reactions. Standard heats of reaction, combustion and formation. Hesss law. Effect of temperature on standard heats of reaction. Steady state energy balance calculations. Steam tables. Module -IV Stoichiometry of microbial growth and product formation. Stoichiometric calculations elemental balances, degree of reduction. Yield coefficients. Biomass yield. Product stoichiometry. Theoretical oxygen demand. Maximum possible yield. Thermodynamics of microbial growth. Heat of reaction with oxygen as electron acceptor and with oxygen not the electron acceptor. Energy balance equation for cell culture. Fermentation energy balance equation.
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References: 1. David M Himmelblau Basic principles and calculations in Chemical Engg Prentice Hall India. 2. V.Venkatarmani & N.N.Ananthraman Process calculation Prentice Hall India. 3. B I Bhatt & S.M Vora Stoichiometry Tata McGraw Hill. 4. Michael L Shuler & Fikret Kargi Bioprocess Engg. Basic Concepts Prentice - Hall India. 5. Pauline M Doran Bioprocess Engineering Principles Academic Press.
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BT 09 307 (P) MICROBIOLOGY LABORATORY

Teaching Scheme: 3 hours practical per week Objectives Credit: 2
To attain knowledge about the morphology and in vitro cultivation of microorganisms To study about the microbial analysis of food water and soil
1. Sterilization techniques; Wet heat, dry heat, filtration, disinfection 2. Preparation of culture media, cotton plugging and sterilization 3. Culturing of microorganisms: broth, agar, pure culture, streak Plate, pourplate,Spread plate isolation and preservation of bacterial culture. 4. Identification of microorganisms: Staining techniques, Simple staining,Gram staining,spore, capsule, fungal staining, andbiochemical testCarbohydrate Fermentation,IMViC, TSI, Urease Test. 5. Quantification of microorganisms: counting microscopy, nephelometry /turbidometry, total N or dry weight. 6. Growth curve of bacteria 7. Microbiological analysis of water, 8. Food microbiology: milk, fermented food. Salmonella in poultry 9. Factors affecting the bacterial growth: effect of temperature and pH 10. Clinical microbiology: Normal mouth flora,Antibiotic Assay 11. Microbial population in soil 12. Isolation of nitrogen fixing organisms. Sessional work assessments Lab Practical and Record Two tests (2 x 10) Regularity Total marks = = = = 60% 30% 10% 50
Semester end examination Fair record Viva voce Procedure and tabulation form, Conducting experiments and results Total marks
= = = =
10% 20% 70 % 50
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BT 09 308 (P) BIOCHEMISTRY LABORATORY

Teaching Scheme: 3 hours per week Objective: To study about the qualitative and quantitative analysis of biomolecules 1. Units, Volume and weight measurements, concentration units, pH measurement, preparation of buffers. 2. Qualitative tests for carbohydrates and amino acids 3. Estimation of reducing sugars by the Benedicts method. 4. Quantitative estimation for amino acids-Ninhydrin method. 5. Protein estimation by Biuret/Folins/Bradford method. 6. Acid hydrolysis of proteins and estimation of amino acids by ninhydrin, OPA and PTH. 7. Extraction of lipids 8. Saponification of fats 9. Estimation of cholesterol 10. Estimation of nucleic acids: Precipitation by sodium sulphate, test for ribose and deoxyribose 11. Hydrolysis of ester using papain 12. Trypsin digestion of proteins Sessional work assessments Lab Practical and Record Test/s Regularity Total marks = = = = 60% 30% 10% 50 Credits: 2
Semester End examination Fair record = Viva voce = Procedure and tabulation form, Conducting experiments and results = Total marks =
10% 20% 70 % 50
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FOURTH SEMESTER EN09 401A: Engineering Mathematics IV (Common for ME, CE, PE, CH, BT, PT, AM, and AN)
Teaching scheme 3 hours lecture and 1 hour tutorial per week Credits: 4
Objective The use of probability models and statistical methods for analyzing data has become common practice in virtually all scientific disciplines. Two modules of this course attempt to provide a comprehensive introduction to those models and methods most likely to be encountered and used by students in their careers in engineering. A broad introduction to some important partial differential equations is also included to make the student get acquainted with the basics of PDE.
Module I: Probability Distributions (13 hours) Random variables Mean and Variance of probability distributions Binomial Distribution Poisson Distribution Poisson approximation to Binomial distribution Hyper Geometric Distribution Geometric Distribution Probability densities Normal Distribution Uniform Distribution Gamma Distribution. Module II: Theory of Inference (14 hours) Population and Samples Sampling Distribution Sampling distribution of Mean ( known) Sampling distribution of Mean ( unknown) Sampling distribution of Variance Interval Estimation Confidence interval for Mean Null Hypothesis and Tests of Hypotheses Hypotheses concerning one mean Hypotheses concerning two means Estimation of Variances Hypotheses concerning one variance Hypotheses concerning two variances Test of Goodness of fit. Module III: Series Solutions of Differential Equations (14 hours) Power series method for solving ordinary differential equations Legendres equation Legendre polynomials Rodrigues formula Generating functions Relation between Legendre polynomials Orthogonality property of Legendre polynomials (Proof not required) Frobenius method for solving ordinary differential equations Bessels equation Bessel functions Generating functions Relation between Bessel functions Orthogonality property of Bessel functions (Proof not required). Module IV: Partial Differential Equations (13 hours) Introduction Formation of PDE Complete Solution Equations solvable by direct integration Linear PDE of First order, Legranges Equation: Pp + Qq = R Non-Linear PDE of First Order, F(p,q) =0 , Clairauts Form: z = px + qv + F(p,q) , F(z,p,q) =0 , F 1(x,q) = F2(y,q) Classification of Linear PDEs Derivation of one dimensional wave equation and one dimensional heat equation Solution of these equation by the method of separation of variables DAlemberts solution of one dimensional wave equation.
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Text books Module I: Richard A Johnson, CB Gupta, Miller and Freunds Probability and statistics for Engineers, 7e, Pearson Education- Sections: 4.1, 4.2, 4.3, 4.4, 4.6, 4.8, 5.1, 5.2, 5.5, 5.7 Module II: Richard A Johnson, CB Gupta, Miller and Freunds Probability and statistics for Engineers, 7e, Pearson Education- Sections: 6.1, 6.2, 6.3, 6.4, 7.2, 7.4, 7.5, 7.8, 8.1, 8.2, 8.3, 9.5 Module III: Erwin Kreysig, Advanced Engineering Mathematics, 8e, John Wiley and Sons, Inc.Sections: 4.1, 4.3, 4.4, 4.5 Module IV: N Bali, M Goyal, C Watkins, Advanced Engineering Mathematics, A Computer Approach, 7e, Infinity Science Press, Fire Wall Media- Sections: 16.1, 16.2, 16.3, 16.4, 16.5, 16.6, 16.7, 16.8, 16.9 Erwin Kreysig, Advanced Engineering Mathematics, 8e, John Wiley and Sons, Inc. Sections: 11.2, 11.3, 11.4, 9.8 Ex.3, 11.5 Reference books 18. William Hines, Douglas Montgomery, avid Goldman, Connie Borror, Probability and Statistics in Engineering, 4e, John Wiley and Sons, Inc. 19. Sheldon M Ross, Introduction to Probability and Statistics for Engineers and Scientists, 3e, Elsevier, Academic Press. 20. Anthony Croft, Robert Davison, Martin Hargreaves, Engineering Mathematics, 3e, Pearson Education. 21. H Parthasarathy, Engineering Mathematics, A Project & Problem based approach, Ane Books India. 22. B V Ramana, Higher Engineering Mathematics, McGrawHill. 23. Sarveswara Rao Koneru, Engineering Mathematics, Universities Press. 24. J K Sharma, Business Mathematics, Theory and Applications, Ane Books India. 25. John bird, Higher Engineering Mathematics, Elsevier, Newnes. 26. M Chandra Mohan, Vargheese Philip, Engineering Mathematics-Vol. I, II, III & IV., Sanguine Technical Publishers. 27. Wylie C.R and L.C. Barret, Advanced Engineering Mathematics, McGraw Hill. 28. V R Lakshmy Gorty, Advanced Engineering Mathematics-Vol. I, II, Ane Books India. 29. Sastry S.S., Advanced Engineering Mathematics-Vol. I and II, Prentice Hall of India. 30. Michael D Greenberg Advanced Engineering Mathematics, Pearson Education.
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University Examination Pattern
PART A: Short answer questions (one/two sentences) All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions Two questions from each module with choice to answer
EN09 402: Environmental Science (Common for all branches)

Objectives To understand the problems of pollution, loss of forest, solid waste disposal, degradation of environment, loss of biodiversity and other environmental issues and create awareness among the students to address these issues and conserve the environment in a better way. Module I (8 hours) The Multidisciplinary nature of environmental science, Definition-scope and importance-need for public awareness. Natural resources, Renewable and non-renewable resources: Natural resources and associated problems-forest resources: Use and over exploitation, deforestation, case studies. Timber extraction, mining, dams and their defects on forests and tribal people.water resources: Use and over utilization of surface and ground water, floods ,drought ,conflicts over water, dams-benefits and problems.- Mineral resources: Use and exploitation,environmental effects of extracting and using mineral resources, case studies.- Food resources: World food problems, changes caused by agriculture over grazing, effects of modern agriculture, fertilizer-pesticide problems, water logging,salinity,case studies.-Energy resources: Growing energy needs, renewable and non-renewable energy resources, use of alternate energy resources, Land resources: Land as a resource, land degradation, man induced land slides, soil erosion and desertification. Module II (8 hours) Ecosystems-Concept of an ecosystem-structure and function of an ecosystem producers, consumers, decomposers-energy flow in the ecosystem-Ecological succession- Food chains, food webs and Ecological pyramids-Introduction, types, characteristics features, structure and function
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of the following ecosystem-Forest ecosystem- Grassland ecosystem Desert ecosystem-Aquatic ecosystem(ponds, streams, lakes, rivers, oceans , estuaries) Biodiversity and its consideration Introduction- Definition: genetic , species and ecosystem diversity-Biogeographical; classification of India value of biodiversity: consumptive use, productive use, social ethical , aesthetic and option values Biodiversity at Global, national , and local level-India at mega diversity nation- Hot spot of biodiversity-Threats to biodiversity: habitat loss, poaching of wild life, man , wild life conflicts Endangered and endemic species of India-Conservation of biodiversity : In-situ and Ex-situ conservation of biodiversity. Module III (10 hours) Environmental pollution Definition-Causes, effects and control measures of Air pollution-m Water pollution soil pollution-Marine pollution-Noise pollution-Thermal pollution-Nuclear hazards-Solid waste management: Causes, effects and control measures of urban and industrial wastes-Role of an individual in prevention of pollution-pollution case studies-Disaster management: floods, earth quake, cyclone and landslides-Environmental impact assessment Module IV (10 hours) Environment and sustainable development-Sustainable use of natural resources-Conversion of renewable energy resources into other forms-case studies-Problems related to energy and Energy auditing-Water conservation, rain water harvesting, water shed management-case studies-Climate change, global warming, acid rain, ozone layer depletion, nuclear accidents and holocaust-Waste land reclamation-Consumerism and waste products-Reduce, reuse and recycling of productsValue education. Text Books 1. Clark, R.S.Marine pollution, Clanderson Press Oxford. 2. Mhaskar A. K. Matter Hazrdous, Techno-science Publications. 3. Miller T. G. Jr., Environmental Science, Wadsworth Publishing Co. 4. Townsend C., Harper J, Michael Begon, Essential of Ecology, Blackwell Science 5. Trivedi R. K., Goel P. K., Introduction to Air Pollution, Techno-Science Publications. Reference Books. 1. Raghavan Nambiar,K Text book of Environmental Studies,Nalpat Publishers Kochi 2. Bharucha Erach, Biodiversity of India, Mapin Publishing Pvt. Ltd., Ahmedabad 380 013, Email: mapin@icenet.net 3. Cunningham, W.P., Cooper, T.H., Gorhani, E & Hepworth, M.T. 2001Environmental encyclopedia Jaico publ. House Mumbai 1196p 4. Down to Earth, Centre for Science and Environment 5. Hawkins, R.E. Encyclopedia of Indian Natural History, Bombay Natural History Society, Bombay 6. Mckinney, M.L. & School, R.M. 1996. Environmental Science system & Solutions, Web enhanced edition, 639p. 7. Odum, E.P. 1971. Fundamentals of Ecology. W.B.Saunders Co. USA, 574p 8. Rao, M.N. & Datta, A.K 1987. Waste Water treatment. Oxford & IBH Publ. Co. Pvt. Ltd., 345p 9. Survey of the Environment, The Hindu Magazine 10. Wagner.K.D. 1998. Environmental Management. W.B. Saunders Co. Philadelphia, USA 499p
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BT 09 403 CHEMICAL REACTION ENGINEERING

Teaching Scheme: 4 Hrs lecture and 1 hr tutorial Objectives: credits: 5
To impart the basic concepts of reaction kinetics To develop knowledge for design of ideal reactors To study about non-isothermal reactor design To study about the fundamentals of non-ideal reactors and heterogeneous catalytic reactors.
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Module I Introduction to chemical reaction engineering. Classification of chemical reactions and reactors. Variables affecting the rate of reaction. Definition of reaction rate. Kinetics of homogeneous reaction. Concentration dependent term and temperature dependent term. Interpretation of batch reactor data. Constant volume batch reactor. Varying volume batch reactor. Module - II Introduction to reactor design. Ideal reactors for a single reaction. Design for single reaction and multiple reactions. Multiple reactor systems. Size comparison of reactors. Recycle reactor. Autocatalytic reactor. Module III Heat effects in reactors. Non isothermal reactor design. General graphical design procedure. Energy balance for batch, mixed flow and plug flow reactor isothermal, adiabatic and nonadiabtic operation. Optimum temperature progression. Multiple steady states, criteria for stability of reactors. Basics of non-ideal flow. Residence time distribution. Measurement of the RTD. Pulse and step input . C,E,F curves. Calculation of mean residence time. RTD in ideal reactors.RTD in laminar flow reactor. Segregation model and conversion in non-ideal reactors ( Dispersion and tanks-inseries models are not included). Module IV Heterogeneous catalytic processes. Classification of catalysts, promoters, inhibitors, catalyst poisons, Adsorption. Rates of adsorption, desorption, and surface reaction. Rate equations for fluid-solid catalytic reactions.. Mass Transfer between fluid and catalyst surface. Internal transport effects. Effectiveness factor for a straight cylindrical catalyst pore for a first order reaction. Commercially significant types of heterogeneous catalytic reactors (description only) like fixed bed reactor, trickle bed reactor, moving bed reactor, fluidized bed reactor, slurry reactor. Text book/References 1. Octave Levenspiel Chemical Reaction Engg Wiley student Education. 2. H.Scott Fogler Elements of Chemical Reaction Engg. Pearson Prentice Hall. 3. J.M.Smith Chemical Engineering Kinetics McGraw Hill International.
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BT 09 404
Teaching Scheme:
BIOCHEMISTRY II
Credits: 4
3 hrs lecture and 1 hr tutorial per week Objectives:
To impart complete understanding of biochemical processes associated with the living cell To enable the students to see how metabolic pathways communicate with each other
Module I Bioenergetics: Redox potential, biological oxidation, respiratory control. Hub of metabolism: glycolysis: reactions, energetics and regulation.TCA Cycle: reactions, energetics and regulation. Gluconeogenesis pathway: Significance, reactions and regulation. Glycogen metabolism. Maintenance of blood glucose levels. Oxidative phosphorylation: Electron transport chain mitochondria and components of electron transport system (Role of complexes I-IV, FIFO ATPase) Chemiosmotic coupling, uncouplers and inhibition of oxidative phosphorylation. Pentose phosphate pathway: significance, reactions and regulation. Module II Metabolism of amino acids: Essential and non-essential amino acids, overview of degradation and biosynthesis of amino acids. Transamination reactions,biosynthesis of aromatic amino acids
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and biosynthesis of cysteine, histidine and serine. linkage with the citric acid cycle. Urea cycle: reactions, regulation and its
Module III Metabolism of lipids: fatty acid oxidation, ketone bodies, ketosis, biosynthesis of fatty acids and triglycerides. Biosynthesis of phospholipids. Biosynthesis of Cholesterol and significance. Module IV Metabolism of nucleic acid: Denovo biosynthesis of Purine and pyrimidine nucleotides, regulation of Purine and pyrimidine nucleotide biosynthesis. Salvage pathways of Purine and pyrimidines. Formation of deoxyribonucleotides. Catabolism of purines and pyrimidines.
Tex books/References 1. Lehninger Principles of biochemistry, David L.Nelson and Michal M Gox, W.H.Freeman and co. 2. Donald Voet, Judit G Voet, Charlotte W Pratt Fundamentals of biochemistry. 3. Lubert Stryer, Biochemistry. W.H.Freeman & Co. 4. Robert K.Murray, Daryl K.Grammer.Harpers Illustrated Biochemistry. McGraw Hill
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BT 09 405
Teaching Scheme: 3 hrs lecture and one hr tutorial per week Objectives:
CELL BIOLOGY
Credits: 4
To impart knowledge of the components of cells and how cells work To study the cells : their physiological properties, their structure, the organelles they contain, life cycle interaction, division and death at microscopic and molecular level
Module I Discovery of cells. Basic properties of cells. Cell theory. Cell complexity. Different classes of cells. Prokaryotic & Eukaryotic system Prokaryotic & Eukaryotic cells. Importance of carbon and water. Plasma membrane structure and function. Cytoplasm and cytoskeleton, Microtubules, microfilaments and intermediate filaments . Module II Structure and functions of Nucleus, Endoplasmic reticulum, Golgi complex, Lysosomes, Peroxisomes, Chloroplast & Mitochondria. Photosynthesis- introduction to photosystems , light reaction and dark reaction. Protein glycosylation, sorting and transport. Module III Overview of the cell cycle, Interphase. Mitosis, Meiosis and cytokinesis. Animal cell and yeast cell division. Cell cycle control and check points. General characteristics of cell differentiation. Historical events in cell differentiation Cytoplasmic determinants. Nucleoplasmic interactions. Stem cell differentiation and its biological importance. Module IV Passive and active transport, Permeases, Na+/K+ pump, ATP ase pumps, Lysosomal & vacuolar membrane, ATP dependent proton pumps. Co-Transport Symport. Antiport. Transport into prokaryotic cells. Endocytosis and Exocytosis. Receptors: Cytosolic, Nuclear & Membrane bound receptors. Examples and types of receptors. Concept of secondary messengers, cAMP, cGMP
Text/References books 1. Geoffrey M cooper and Robert E Hansman the cell A molecular approach. ASM press. 2. De Robertis and De Robertis. Cell and Molecular Biology Waverly Pvt Ltd. 3. Gerald Karp Cell and Molecular Biology Wiley publishers. 4. Kimball.T.W Cell Biology Wesley publisher. 5. Becker, Kleinsmith, Harden The world of Cell. Pearson Publishers.
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B T 09 406 MECHANICAL OPERATIONS

To impart the basic concepts of size reduction and the knowledge about the size reduction equipments To study about the various mechanical separation operations
Module I Characterization of solid particles. Sieve analysis, particle size distribution , cumulative and differential methods of analysis,mean diameter, specific surface area Microscopic counting, pipette analysis. hydrometer analysis. Size reduction, equipment used for primary and secondary stage size reduction. jaw crusher, hammer mill, ball mill, rod mill, disk attrition mill, fluid energy mill. Laws of size reduction. Selection of equipments. Industrial screen, effectiveness of screens, capacity of screens. Closed and open circuit grinding. Wet and dry grinding. Module II Filtration. Constant rate filtration and constant pressure filtration. Batch and continuous filtration. Sand filter, plate and frame filter press, leaf filter, rotary vaccum filter, incompressible cake, cake porosity, filter aids, methods of application, optimum time cycle. Principle of centrifugal filtration. Suspended batch centrifuge. Automatic batch centrifuges. Module III Sedimentation. Settling theory. Equipment for sedimentation thickeners. Clarifier and thickener design. Kynch theory. Determination of thickener area. Sedimentation principles. . Equilibrium sedimentation. Sedimentation coefficient. Equivalent time. Production centrifuges tubular bowl centrifuges. Ultra centrifugation. Flocculation and sedimentation. Disk stack bowl centrifuge centrifugation theory. Cell recovery in a disk-stack centrifuge. Module IV Cross flow filtration (CFF) Micro filtration and ultra filtration. Applications of CFF. Types of membranes permeate flux for ultra filtration, concentration polarization in ultra filtration, Micro filtration. Filter media in cross flow filtration. Equipments in cross flow filtration. Membrane fouling. Scale up and design of cross flow filtration. Batch and continuous ultra filtration system.
Text/References McCab&Smith: Unit operations in Chemical Engg McGraw Hill Christe J Geankoplis Transport Process and Unit Operations. Prentice Hall of India. Badger and Benjaro, Introduction to Chemical Engineering , Mc Graw Hill
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BT 09 407 (P)
Teaching Scheme: 3 hours practical per week Objective:
FLUID MECHANICS & MECHANICAL OPERATIONS LAB

Credits 2
To study about various fluid flow experiments, particle analysis and mechanical separation experiments
1. Characteristic curves of a centrifugal pump and determination of maximum efficiency. 2. Determination of coefficient of discharge of orifice meter and venturi meter. 3. Drag coefficient of a falling sphere in fluid. 4. Pressure drop of liquid passing through a packed bed. 5. Pressure drop characteristics of a fluidized bed. 6. Losses in pipe fittings, expansion and contraction. 7. Ball mill verification of the laws of crushing.
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8. Sieve analysis-determination of particle size. 9. Effectiveness of a screen 10. Pipette analysis 11. Determination of the mean specific cake resistance and compressibility factor using a batch leaf filter. 12. Batch sedimentation in a slurry 13. Flocculation screening 14. Cross flow filtration. 15. Study of Jaw crusher 16. Study of rotary vaccum filter.
Sessional work assessments Lab Practical and Record Test/s Regularity Total marks
= = = =
60% 30% 10% 50
Semester End examination Fair record Viva voce Procedure and tabulation form, Conducting experiments and results Total marks
= = = =
10% 20% 70 % 50
BT 08 408(P)
BIOANALYTICAL TECHNIQUES LAB

credits 2
To provide experience in various bioanalytical techniques in biotechnolog y

1. Precision, accuracy and validity of an experiment. Analysis and presentation of data. 2. Colorimetry and spectrophotometry 3. Verification of Beer-Lamberts law-using UV-Vis spectrophotometer. a. Change in absorbance with concentration of potassium permanganate. b. Absorption maxima change in absorbance in potassium permanganate with wavelength.
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c. Concentration of two components in a binary mixture. Absorption of light by potassium dichromate and potassium permanganate. d. Change in absorbance of albumin and DNA solution with wave length. Absorption spectra of nucleotides Separation of amino acids by paper chromatography Determination of Rf value. Extraction of lipids and separation using thin layer chromatography Column chromatography Determination of molecular weight of an enzyme by gel filtration. Separation of protein by SDS page. Separation of nucleic acids by agarose gel electrophoresis Biotin labeling of cellular constituents.
4.
5.
6. 7.
8. 9.
10. 11.
= = = =
60% 30% 10% 50
= = = =
10% 20% 70 % 50
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FIFTH SEMESTER
BT09 501 MASS TRANSFER OPERATIONS

To impart the basic concepts of mass transfer To develop an understanding of different separation processes like distillation,extraction,drying and crystallisation
Module-I Classification of mass transfer operations Diffusional mass transfer Ficks law. One component transferring to non-diffusing component and equimolar counter diffusion diffusivity estimation. The mass transfer coefficient. Dimensionless groups in mass transfer. Theories of mass transfer. Film theory, Penetration theory, surface renewal theory, the boundary layer theory. Momentum, heat and mass transfer analogies. Interphase mass transfer. The two-film theory, the overall mass transfer coefficient. General features of equipment for mass transfer Tray tower, packed tower, bubble column, wetted wall tower, and spray tower. Module II Basic concepts of distillation vapor liquid equilibrium, the Raoults law, Relative volatility, Deviations from ideality. Flash vapourisation of a binary mixture, simple distillation, steam distillation, continuous fractionation, McCabe-Thiele method, Ponchon, - Savarit method, minimum reflux ratio. Total reflux, optimum reflux ratio. Module III Liquid liquid extraction applications, Liquid liquid equilibrium. Solvent selection, Design calculations for stage wise extraction, single stage and multi stage operation, crosscurrent and countercurrent operations, liquid liquid extraction equipment. Solid liquid extraction, (leaching). Contacting equipments. Solid liquid extraction equilibrium. Batch contact. Crosscurrent and countercurrent extraction calculations. Super critical fluid extraction. Module-IV Drying of wet solids. Classification of drying equipment. Drying equipment. Drying calculations cross circulation drying and through circulation drying. Material and energy balance in a continuous dryer. Freeze drying. Drying time in a continuous counter current dryer. Crystallization. Principles of crystallization. Solid-liquid phase equilibrium, Nucleation and crystal growth. Effect of impurities on crystal formation Fractional crystallization, caking of crystals. Crystallization equipment. Adsorption: Adsorption Isotherm, Freudlich, Langmuir and BET equation. Application to single stage and multistage operation.
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Text book/References 1. Treybal Mass Transfer operations McGraw Hill International 2. McCabe-Smith Unit operations of Chemical Engg McGraw Hill International 3. Binay K Dutta Mass Transfer and Separation processes. 4. Badger and Benjaro, Introduction to Chemical Engineering, Mc- Graw Hill
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BT 09 502 MOLECULAR BIOLOGY

Teaching Scheme: 3 Hrs Lecture and 1 hr tutorial per week Objectives: Credit: 5
To get a proper understanding about the central dogma of the molecular mechanism To study the analytical techniques used in molecular biology
Module I Introduction to genetics: Mendelian inheritance. Segregation at one locus. Segregation at two loci genetic mapping. Structure of DNA. Nucleosome. Chromatin organization. Chromosome structure. Plasmids types of plasmid. Structural differences between DNA and RNA. DNA replication: Enzymes involved. Mechanism of replication. Regulation damage and repair, rolling circle replication. Plasmid replication Telomerase. Module II Concept of the gene: Gene structure and architecture. Central dogma. Genetic code: An overview of genetic code Fidelity of translation, Wobble hypothesis. Ambiguity of genetic code. Deviation from standard. Genomes and mapping :Genomes, ploidy and chromosome number. Physico-chemical property of the genome. Genome organization, gene mapping. Physical mapping. Comparative genomics. Module III Gene transfer in bacteria: conjugation, transformation, transduction. Gene expression. Role of RNAs, enzymes and other factors. Mechanism of transcription and translation in prokaryotes and eukaryotes. Post- translational modifications. Regulation of gene expression regulation of transcription. Different mechanisms Regulatory proteins. Induction and repression. Concept of operon. . Examples (Lac and Trp operon) Positive and negative regulations. Post-transcriptional regulation. Module IV Mutation: Mutagenesis. Types of mutation. Classes of mutagens Replica plating. Mutation in genetic analysis. Site directed mutagenesis Molecular biology Techniques Electrophoresis (Agarose gel and PAGE). Blotting techniques (Southern, Northern, Western) PCR and variants.
Text Books/References 1. Freifelder Molecular Biology James and Bartletyt Publishers. Ire 2. Banjamin Levin: Genes VI, Oxford University Press, Oxford, New York. 3. Bruce Albert and James, D Watson: Molecular Biology of the cell. Garland publishing.
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BT09 503 ENZYME SCIENCE & ENGG.

Teaching Scheme: 3 Hrs lecture and 1 hr tutorial per week Objective: Credit: 4
To impart the basic concepts of enzymes and the reactors involved in free and immobilized enzyme system To understand the kinetics and physicochemical characteristics of enzymes
Module I Classification of enzymes, commercial application of enzymes in food, pharmaceutical and other industries. Enzymes for analytical and diagnostic applications. Production and purification of crude enzymes. Extracts from plant, animal and microbial sources. Methods of
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characterization of enzymes. cellulose using enzymes. Development of enzymatic assays. Hydrolysis of starch and
Module-II Mechanism of Enzyme action, Concept of active site, enzyme-substrate complex and enzyme action, Simple enzyme kinetics with one and two substrates. Michaelis-Menten kinetics. Evaluation of parameters in the Michaelis-Menten kinetics Equation. Types of inhibition. Influences of pH, temperature, fluid forces, chemical agents and irradiation on enzyme activity, deactivation kinetics. Module III Enzyme immobilization. Physical and chemical techniques for enzyme immobilization adsorption, matrix entrapment, encapsulation, cross linking, covalent binding. Advantages and disadvantages of different immobilization techniques. Over view of application of immobilized enzyme systems. Mass transfer effects in immobilized enzyme systems. Analysis of film and pore diffusion effects on kinetics of immobilized enzyme reactions. Effectiveness factors. Module IV Batch Operation of a stirred reactor Time course for batch enzyme reaction. Continuous operation in a stirred tank reactor. Immobilized enzyme reaction in a CSTR and plug flow reactor. Design of immobilized enzyme reactors packed bed, fluidized bed and membrane reactors. Enzyme biosensors, application of enzymes in analysis, design of enzyme electrodes and their application in industry, health care and environment.
Text books/References 1. Zubay G, Biochemistry, Maxwell Macmillon International Education 2. Gerharts, W, Enzymes in industry Production and application. 3. Tailer, R.F. Protein Immobilization Fundamentals and applications. 4. Pauline M Doran Biopriocess Engg. Principles Academic press 5. James E Bailey & David F Ollis Biochemical Engineering Fundamentals Mc Graw Hill Book Company.
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PART A: Short answer questions (one/two sentences) All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions
Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions Two questions from each module with choice to answer 4 x 10 marks=40 marks
BT 09 504
THERMODYNAMICS AND HEAT TRANSFER

Credit: 4
Teaching Scheme: 3 Hrs lecture and 1 hr tutorial per week Objective:
To impart the basic concepts of thermodynamics and heat transfer To study the design of various types of heat exchangers
Module I Introduction and fundamental concepts of thermodynamic terms. First law of thermodynamics. Work equivalent of heat. Classification of energy, thermodynamic state function and path function. Enthalpy and specific heat. Application of first law to steady state flow processes and reversible process. Limitations of first law. Statement of second law. Entropy. Heat reservoirs, heat engines and heat pumps. Applications of the laws of thermodynamics to refrigeration, liquefaction process. Module II Modes of heat transfer: conduction, convection and radiation. Heat transfer by conduction. Fouriers law. Thermal conductivity Steady state conduction through a composite solid. Steady state conduction through cylinder and spheres Heat transfer coefficient: Convective heat transfer and the concept of heat transfer coefficient. Overall heat transfer without heat transfer between fluids separated by a plane wall. Heat transfer between fluids separated by a cylindrical wall.
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Module III Forced convection. Flow over a flat plate. Thermal boundary layer. Dimensionless groups in heat transfer. Correlations for the heat transfer coefficient. Laminar flow through a circular pipe. Turbulent flow through a circular pipe. Flow through a non-circular duct. Heat transfer with cocurrent and counter current operations. LMTD. Momentum and heat transfer analogy. Radiation heat transfer basic concepts. Blackbody radiation. Plancks, Wiens, Stefan Boltzmann and Kirchoffs Laws. Grey body. Boiling liquids and condensing vapours basic ideas. Module IV Heat exchangers. Construction of a shell and tube heat exchanger. Fouling of a heat exchangerthe fouling factor. Design of a double pipe heat exchanger. Design of shellandtube heat exchanger. Cleaning of heat exchangers. Compact heat exchangesplate heat exchangers, and spiral heat exchangers. Thermal design of an agitated vessel. Design of cooling coils. Steam jacketing and heat transfer fluids.
Text book/References 1. Binoy K Duttaa Heat Transfer Principles and applications Prentice - Hall of India. 2. McCabe and Smith Unit operations of Chemical Engg McGraw Hill International editions. 3. Badger and Banchero Introduction to Chemical Engg. McGraw Hill International Edition. 4. Kern D Q, Process Heat Transfer, Mc Graw Hill 5. Smith J M Van Ness H V, Introduction to Chemical Engg Thermodynamics, Mc Graw Hill 6. J P Holman, Heat Transfer ,9th edn , Tata Mc Graw Hill Edn
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BT 09 505
Teaching Scheme:
BIOINFORMATICS
Credits: 4
3 hours lecture and 1 hour tutorial per week Objective:
To understand the fundamental principles of bioinformatics and using the knowledge to tackle various research problems in molecular biology
Module I Scope of Bioinformatics Internet basics, www, protocols FTP Telnet HTTP, Elementary commands in UNIX, Introduction to PERL (Basic commands, pattern and string matching), BIOPERL (Programme, to transcribe and translate nucleotide sequences). Installation of bioperl and its applications. Module II Introduction of databases, Biological databases and their ruse, Databanks nucleotide databanks Genbank, NCBI, EMBI, DDBJ Protein databanks sequence databanks PIR, SWISSPROT, TrEMBL structural databases PDB, SCOP, CATH, SSEP, CADB, Pfam and GDB. Data base search, sequence submission, Sequin, Bankit, Sakura, Database search FASTA-BLAST.
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Module III Sequence alignment, Pairwise alignment Dynamic programming, Multiple sequence alignment. Methods of MSA, Clustal W, Phylogenetic analysis. Tree building methods Distance, Parsimony, Maximum likelihood, Phylogenetic Software, Phylip. Scoring Matrix PAM, BLOSUM. Module IV Special topics in bioinformatics Methods for prediction of secondary and tertiary structures of proteins knowledge based structure prediction: fold recognition, ab initio methods for structure prediction, Comparative protein modeling, Gene predictions, Genscan, Precustes, Grail, DNA Mapping and sequencing. Map alignment Shotgun DNA sequencing Sequence assembly. Protein structure visualization. Reference: 1. Andreas D.Baxevanis, B.F.Francis Oouellette, Bioinformatics, Wiley. 2. Dam Gusfield. Algorithms on Strings Trees and Sequences, Cambridge University Press. 3. D.Mount, Bioinformatics Sequence Analysis. Cold Spring Harbor Laboratory.
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BT 09 506
Teaching Scheme:
PROCESS INSTRUMENTATION
Credits: 3
2 hours lecture and 1 hour tutorial per week Objectives:
To impart the basic ideas about instruments To evaluate the operating principles of different instruments To study the instrumentation and control of bioprocesses
Module I Introduction Definition of instrumentation Concept of an instrument. Functional elements and functions of an instrument Classification of instruments. Static and dynamic characteristics of measuring instrument. Accuracy, reproducibility, sensitivity, static error, dead zone, dynamic error, fidelity, lag, speed of response. Sensing elements - various types, sensors for temperature, pressure and fluid flow, transducers, their principles and working, transmission methods, indicating and recording means. Temperature measurements, temperature scales, basic principles and working of thermometers-mercury-in-glass thermometers, resistance thermometers, thermocouples, optical pyrometers, radiant pyrometers, ranges of different types of temperature measuring instruments. Sources of errors and precautions to be taken in temperature measurements. Module II Pressure measurement. Principles of working of manometers. Various types of manometers. McLeod gauge, Knudsen gauge, bourdon gauge, bellow, diaphragm. Transducers of electrical and mechanical types. Piezo-electric manometers, thermal conductivity gauges, ironisation gauge, high pressure measuring instrument. Density measurement using constant volume hydrometer and air pressure balance method Gas density detector and gas specific gravity measuring system. viscosity measurement. Measurement of pH, Liquid level measurements. Module III Flow measurement using head type flow meters based on differential pressure measurement. Orifice meter, venturimeter, flow nozzle and pitot tube. Electromagnetic flow meters. Variable area meters like rotameter. Mechanical flowmeters of positive displacement type like rotating disk and turbine type and anemometers. Moisture content determination by thermal drying. Instruments for measuring humidity like hygrometer, psychrometer, and dew-point apparatus. Composition analysis using spectroscopic methods like absorption, emission and mass spectrometers. Gas analysis by thermal conductivity, polarography and chromatography. Module IV Instrumentation for bioprocesses. Physical and chemical sensors for the medium and gases. Medium chemical sensors. Gas analysis. On line-sensors for cell properties. Offline
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analytical methods. Measurement of medium properties. Analysis of cell population composition. Flow cytometry. Data analysis, data smoothing and interpolation. State and parameter estimation. Development of P&I diagrams for flow systems, level, PH control. Temperature control, heat exchangers, distillation columns and reaction systems. Text Books/References 1. D.P.Eckman. Industrial instrumentation, Wiley Eastern 2. Fribance, Industrial Instrumentation fundamentals, TMH Edition 3. R.K.Jain. Mechanical and Industrial Measurements, Khanna Publishers. 4. Bailey & Ollis. Biochemical Engineering Fundamentals McGraw Hill Book Company 5. Beckworth and Buck: Measurement Systems
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BT 09 507(P)
HEAT AND MASS TRANSFER LAB

Credits: 2
To study about the experiments in heat and mass transfer

1. Conduction -Determination of thermal conductivity 2. Heat transfer through composite wall 3. Heat transfer in natural conviction. 4. Heat transfer in forced convection 5. Double pipe heat exchanger 6. Shell and tube heat exchanger 7. Simple distillation 8. Steam distillation 9. Sieve place distillation column 10.Solid -liquid extraction Bonnotto type 11.Solid-liquid extraction- packed bed type 12.Ternary liquid equilibrium 13. Leaching simple leaching, cross current and counter current leaching. 14. Rotary dryer 15. Adsorption isotherms 16. Batch crystallizer
= = = =
60% 30% 10% 50
Semester End Examination Fair record Viva voce Procedure and tabulation form, Conducting experiments and results Total marks
= = = =
10% 20% 70 % 50
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BT 09 508 (P)
Teaching Scheme: 3 hours practical per week Objectives:
BIOPROCESS ENGINEERING LAB

Credits: 2
To do experiments based on enzymes and microbial cells To study about bioreactors and mass transfer effects
1. Enzyme isolation and assay of enzymatic activity. 2. Estimation of Michaelis Menten parameter effect of pH and temperature on enzyme activity. 3. Techniques of enzyme immobilization 4. Bio-conversion studies with immobilized enzyme reactors. 5. Culturing of different types of micro organisms (Bacteria, Yeast, fungi) used in the production of commercially imported products. 6. Formulation of simple and complex culture media 7. Estimation of biomass, substrate and product analysis 8. Study of growth, substrate utilization and product formation kinetics in shake flask cultures 9. Growth of micro organisms-estimation of Monod parameters 10. Production of secondary metabolite in synthetic and complex industrial media. 11. Mass transfer rate determination in bio reactors dynamic gassing out method. 12. Oxygen transfer rate in diffused air system (aeration unit) 13. Aerobic bioreactor 14. Anaerobic bioreactor 15. Screening of process variable-Single dimensions research, Plackett Burman design 16. Study of rheology of fermentation broth and power determination
= = = =
60% 30% 10% 50
= = = =
10% 20% 70 % 50
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SIXTH SEMESTER BT 09 601 GENETIC ENGINEERING

Teaching Scheme: 4 hours lecture and 1 hour tutorial per week Objectives: Credits: 5
To impart the basic concepts of genetic engineering To impart the knowledge of various techniques involved in genetic engineering
Module- I Introduction to genetic engineering. Tools of genetic engineering. Restriction enzymes. DNA modifying enzymes. Principles of molecular cloning. Sources of DNA for cloning. Isolation of DNA. Mechanism of DNA cleavage, and end modifications. Mechanism of DNA joining. CDNA cloning. Screening strategies. Module - II Salient features of vectors. Plasmids (plasmid biology, purification of plasmid DNA, Desirable properties of plasmid closing vehicles) Bacteriophages (Bateriophage ) others (cosmids BACS, PACS, YACS) Eucaryotic vectors. Expression vectors. Shuttle vectors. Ti plasmid of agrobacteria. DNA transfer to host: Chemical transfection, lipofection, electroporation, microinjection, gene gun, uses of viruses. Module - III Expression of closed genes: Rationale for expression. Stability of expression vector. Functionability of expression system. Expression signals. Expression strategies. Native and fusion proteins. Construction of libraries: c DNA library, c DNA synthesis, proportion, construction uses. CDNA library Vs genomic DNA library. Sequencing; Maxim Gilbert sequencing, Sanger sequencing, Shortgun approach. Module - IV Application of r DNA technologies: Nucleic acid sequences as diagnostic tools. Applications in SAGE, EST, FISH, Micro array, Restriction mapping, blot analysis southern, Northern and western blot. PCR and its applications. Molecular markers: RFLP, RAPD, AFLP, gene cloning in medicine (insulin Blood clotting factor VIII) High level.
Text Books/References Old RW, Primose SB Principles of gene manipulation-An introduction to genetic engineering. Blackwell Scientific Publications, 1993. T.A.Brown. gene cloning. B.R.Glick & Jack J Pasternak Molecular Biotechnology.
BT 09 602 BIOPROCESS ENGINEERING

Teaching Scheme: 3 hours lecture and 1 hour tutorial Objectives: Credits: 4
To impart knowledge on the design analysis, monitoring, modelling and simulation aspects of a bioreactor To strengthen the knowledge of the design, operation and stability analysis of a bioreactor
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Module - I Introduction to fermentation process. Batch growth. Quantifying cell concentration Kinetics in batch culture. Environmental conditions on growth. Monod model. Growth kinetic with plasmid instability. Product formation kinetics. Structured and unstructured models. Thermal death kinetics of cell and Spores. Continuous culture. Monod chemostat model. Module - II Media design for fermentation Medium requirements and formulation. Antifoams. Medium optimization. Medium sterilization batch and continuous sterilization. Spiral heat exchanger. Sterilization of air. Inoculum preparation for industrial fermentation operation of a typical aseptic aerobic fermentation process. Module - III The oxygen requirements of industrial fermentation. Oxygen supply. The determination of KLa values . The sulphite oxidation method. Gassing out method. Factors affecting oxygen transfer rate in fermenters like bubble size, gas hold-up, gas velocity, temperature, pressure etc. Power required for sparged and agitated vessels. The relationship between power consumption and operating variables. Role of shear in stirred fermenters. The structural components of the fermenter involved in aeration and agitation. Module - IV Basic functions of a fermenter. Bioreactor configurations. Design of stirred batch fermenter, chemostat, chemostat with cell recycle, fed batch fermenter. Plug flow reactor, packed bed, bubble column, fluidized bed bioreactor, trickle bed reactor, Immobilized cell bio-reactor. Air lift formenter. Reactors for animal cell and plant cell. Scale up of fermentors. Solid state fermentation Text Books/References 1. P.F Stanbury, A. Whitaker And S.J Hall. Principles of Fermentation Technology Aditya Books (P) Ltd, New Delhi. 2. James E Bailey & David F Ollis Bio chemical Engineering Fundamentals Second Edition. McGraw Hill Book Company. 3. Pauline M Doran Bio process Engineering Principles Academic Press 4. Michael L Shuler & Fikret Kargi. Bio process Engineering Basic concepts. Prentice Hall of India Pvt Ltd.
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BT 09 603 FINANCIAL MANAGEMENT AND COST ESTIMATION OF PROCESS INDUSTRIES

Teaching Scheme: 3 hours lecture and one hour tutorial per week Objectives: Credits: 4
To impart the basic concepts of financial management of process industries To impart knowledge on the cost estimation based on plant and products
Module - I Overview of financial management. Financial decisions. Capital budgeting decision. Financing decision. Dividend decision. Current asset management. Time value of money and equivalence. Compounding and discounting. Current worth and future worth of cash flows. Interest: simple interest. Compound interest. Continuous interest. Nominal and effective interest rates cost of capital. Depreciation and taxes. Nature of depreciation. Method of determining depreciation. Straight line method. Sinking fund method. Declining balance method. Double declining balance method. Sum of digits method. Units of production method. Comparison of depreciation methods. Taxes. Effect of taxes on depreciation methods. Taxes. Effect of taxes on
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depreciation method. Present worth after taxes. Inflation. Cost comparison under inflation. Capital recovery factor capitalized cost.
Module - II Capital budgeting appraisal methods. Investment evaluation. Mathematical methods for profitability evaluation. Pay back period. Accounting rate of return. Discounted cash flow methods. Net present value. Internal rate of return. Payback reciprocal and rate of return. Profitability index. Comparison between NPV and IRR methods. Ranking mutually exclusive projects, Capital rationing. Financing decision. Financial leverage. Effect of financial leverage on share holders earnings. Earnings per share. Return on equity. EBIT EPS analysis Graphic representation. Risk and financial leverage. Measures of financial leverages. Module - III Capital requirements and cost of production of process plants. Fixed capital and working capital. Estimation of capital investment. Direct costs and indirect costs. Types of capital cost estimated cost indices. Nelson refinery construction index. Material cost indices. Process equipment cost index. Labour cost index. Equipment costs. Williams six-tenth factor. Cost components in capital investment. Purchased equipment. Installation Instrumentation and controls. Piping. Electrical items. Buildings. Yard improvements. Service facilities. Health safety and environment functions land and development. Engineering and supervision. Legal expenses. Construction expenses. Contractors fee. Contingencies. Methods of estimating capital investment. Estimation of revenue. Estimation of total product cost. Manufacturing costs General expenses. Direct production costs. Administrative expenses. Distribution and marketing costs. Research and development costs. Module - IV Profitability. Breakeven and minimum cost analysis. Types of costs. Variables and fixed costs. Economic production charts. Differential analysis of economic production charts. Critique in the use of break-even and minimum cost analysis. Financial statements. Balance sheet. Profit and loss account. Ratios used for analyzing balance sheet and profit and loss account. Text Books/Reference books. 1. Petere & Timmerhaus Plant Design and Economics for chemical Engineers edition, McGraw Hill 2. I.M Pandey Financial Management . Vikas Publishing House Pvt Ltd. 3. F.C Jellen Cost and Optimization Engineering. 4. Schweyer Process Engineering Economics 5. Vilbrandt & Dryden Chemical Engg Plant Design.
5th
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PART A: Short answer questions (one/two sentences)
All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions 4 x 5 marks=20 marks
BT 09 604 PROCESS DYNAMICS AND CONTROL

To impart the basic ideas of chemical process control To study the methods of analysis of process systems
Module I General introduction of a process control system. Classification of variables in a chemical process. Design elements of a control system. Control aspects of a complete chemical plant. Introduction to mathematical Modelling. Basics and examples of mathematical modelling. Linearization of non-linear system (both single variable and many variables). Deviation variables. Laplace transforms - transforms definitions. Lalace transforms of derivatives and integrals. Problems. Solution of linear differential equations using Laplace transforms. Inversion of Laplace transforms. Transfer functions. Poles and zero of a transfer function. Qualitative analysis of the response of a system. Dynamic behaviour of first order systems. Study of different first order systems. Problems. Dynamic behaviour of higher order systems. Different examples
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Module II Concept of feedback control. Types of feedback controllers. Block diagrams. Effect of proportional integral, derivative and composite control. Control actions on the dynamic response of a system. Notion of stability. Characteristic equation. Routh Hurwitz criterion for stability. Problems. Root Locus analysis. Module III Design of feedback controllers. Brief outline. Simple performance criteria Timeintegral performance criteria, selection of the type of feedback controller. Controller turning ZN tuning. Cohen Coon Tuning. Problems. Frequency response analysis of linear process. Bode diagram, Nyquist plots. Bode stability criteria. Nyquist stability criterion. Problems. Module IV A general introduction to advanced control systems. Dead time compensation, inverse response, cascade control, selective control systems, split-range control, feed forward control, ratio control, adaptive control, inferential control. Introduction to direct digital control systems. Supervisory control. Distributed control system. Process control in bioprocess systems. Direct regulatory control. Cascade control of metabolism. Advanced control strategies. Programmed batch bioreactor. Design and operating strategies for batch plants and continuous process control. Text Books/References Stephanopoulos Introduction to Chemical process Control. Coughanower & Koppel Process Systems analysis and Control. James E Bailley & David F.Ollis, Biochemical Engg. Fundamentals Mc Graw Hill.
Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class University Examination Pattern
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BT 09 605 FOOD BIOTECHNOLOGY

Teaching Scheme: 2 hours lecture and 1 hour tutorial per week Objectives: Credits-3
To impart the basic concepts of food biotechnology To impart knowledge on food processing and preservation techniques and packaging techniques
Module - 1 Role of micro organisms in manufacture and spoilage of fermented products, Cereals, Pulses, Nuts and Oil seeds, Fruits and Fruit products, Vegetables and Vegetable Products, Fish and Meat products. Adulteration in foods milk, pulses and others, Rules and regulations Module - II Microbiological role in food process operation and production: new protein foods: SCP; mushroom; food yeasts, algal proteins. Fermentation as a, method of preparing and preserving foods. Food additives like colouring, flavors and vitamins. Organisms and their use in pickling, alcoholic beverages and other products. Module - III Mechanism of enzyme functions and reactions in process techniques: starch and sugar conversion process or baking by amylases; de-oxygenation and desugaring by glucose oxidase; beer mashing and chill- proofing or cheese making by proteases and various other enzymes, catalytic actions in food processing. Process wastes: whey; molasses; starch substances and other food wastes for bioconversion to useful products. Module - IV Introduction to Food Packaging, interaction of food material with packaging material, preservation of food products. Genetically modified and transgenic food development processing- nutritional and economic aspects.
Reference Books 1. Roger A., Gordon B., and John T., Food Biotechnology 2. Lindsay, Willis, Biotechnology, Challenges for the flavour and food industries, Elsvier Applied Science. 3. W.C. Frazier: Food Microbiology (II edition or later) Mcgraw Hill Book Company, New York (1968) 4. Gustavo F Gutierrez-Lepez, Gustavo V. Barbosa-Canovas Food Science and Food
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PART A: Short answer questions (one/two sentences) All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions Two questions from each ELECTIVE 1 module with choice to answer
BT 09 L01
Teaching Scheme:
PLANT BIOTECHNOLOGY
Credits: 4
To understand the important aspects of biotechnology and plant science To make the students understand the concepts of transgenic plants and the application of gene based techniques
Module - I An outline of molecular biology: DNA Replication, Transcription and fundamentals of recombinant DNA technology, Gene regulation. Agrobacterium and plant genetic engineering: Agrobactrium medicated gene transfer and cloning. Types of plant vectors and their use in gene manipulation
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Module - I An outline of molecular biology: DNA Replication, Transcription and fundamentals of recombinant DNA technology, Gene regulation. Agrobacterium and plant genetic engineering: Agrobactrium medicated gene transfer and cloning. Types of plant vectors and their use in gene manipulation Module - II Plant viruses: Classification diagnosis remedy- viruses as a tool to deliver foreign DNA. Developmental aspects of rhizobium: Legume Symbiosis, Symbiotic: Nitrogen fixation. Regulation of nif and mod gene. Module - III Molecular aspects of diseases susceptibility and resistance: Transposable elements, factors influencing disease resistances and susceptibility RFLP. Transgenics, herbicide tolerance, insect resistance, viral resistance stress tolerance development of diseases resistance plants by introducing Bacillus thuringiensis genes. Module IV Fundamentals of plants tissues culture, plant regeneration organogenesis. Role of tissue culture in rapid clonal production, production of pathogen free plants and synthetic seeds. Protoplast technology: isolation: culture and plant regeneration, protoplast fusion, identification and characterization of somatic hybrids, application of protoplast technology. Hairy root culture.
References Dodds J.H Plant Genetic Engineering, Cambridge University Press Mental S.H, Mathews J.A, Mickee R.A Principles of Plant Biotechnology an Introduction to Genetic Engineering in Plants. Blackwell Scientic Publications.
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Internal Continuous Assessment (Maximum Marks-30) University Examination Pattern
BT 09 L02
Teaching Scheme:
ANIMAL BIOTECHNOLOGY
Credits: 4
3 hours lecture 1 hour tutorial per week: Objectives:
To understand the important aspects of biotechnology used in animal science To understand about the production of animal cell based vaccines and the concept of transgenic animals
Module - I Animal Biotechnology and its scope. History and development of cell culture. Manipulation of reproduction in animals and improvements of livestock Artificial insemination. In vitro fertilization technology.
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Module - II Physical requirements for growing animal cell culture. Culture media for animals cell culture BSS, Serum containing and Serum free media- advantages and disadvantages, growth factors, Initiation of cell culture. Isolation and desegregation of explants. Development of primary culture. Growth curve of animal cell in culture. Commonly used cell lines. Preservation and characterization of animal cells. Cytotoxicity and viability assays. Module - III Organ culture techniques, advantages and applications. animals cells. Transplantation of cultures cells. Methods for cell general methods for production, advantages and ethical issues. selection and antibiotic resistances. Hybridoma technology of antibodies. Introduction to cloning and gene technology. Methods for Transfection of fusion. Transgenic animalsSelectable markers, HAT production of monoclonal
Module- IV Production of vaccines and special secondary metabolic (insulin, growth hormones and interferons, T- plasminogen activator, blood factor VIII). Scaling up of animal cell cultures. Bioreactors for animal cell culture and its optimization.
Reference Books Bernur R Pasternak J.J., Molecular Biology, principles and Applications in recombinant DNA, Panimia Publishing Cooperation. M.M Ranga, Animal Biotechnology, second Edition, Agrobios India M.M. Ranga, Tansgenic animals, Agrobios India. P.R Yadav, rajiv Tyagi, Biotechnology of Animals tissues, Discovery Publishing House R.C Dubey, A Text Book of Biotechnology, S Chand & Company.
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BT 09 L 03
Objectives:
PROTEIN ENGINEERING
Credits: 4
3 hours lecture and 1 hour tutorials per week
To impart advance knowledge on how to engineer proteins through a detailed study of protein structure, its characteristic properties and its significance in biological systems
Module - I Protein Bond interactions in protein structure; primary structure and its determination; secondary structure and its prediction methods; tertiary structure and domain in proteins; proteins folding pathways; quaternary structures; methods to determine 3D structures; X-ray crystallography and NMR method; post translational modifications. Module - II DNA binding proteins; Prokaryotic transcription factors, Helix-turn-Helix motif in DNA binding, Eucaryotic transcription factors, zinc fingers; Membrane proteins; General Characteristics, Transmembrane segments, Bacteriorhodopsin and photosynthetic reaction centres.
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. Module - III Epidermal growth factor. Insulin and PDGF receptors and their interactions with effectors: Protein phosphorylation; immunoglobulins; classes and its biological functions; Enzymes serine proteases, Ribonuclease, Lyzozyme. Module - IV Protein design principles and examples; Methods in Proteins engineering; Immunotoxins; mechanism and its applications; Drug designing; structure based approach, receptor based approach.
References: 1. Moody PCE and AJ Wilkinson Protein Engineering IRL Press oxford 2. Cerighton TE Proteins, Freeman WH. 3. Branden C Toozer R Introduction of Protein structure. Garland 1993. 4. Voet D., Voet G., Bio chemistry , III Edition, John Wiley and Sons, 2001 5. Walsh, Protein Biotechnology and biochemistry, 2nd ed., Wiley Publications. Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
PART A: Short answer questions (one/two sentences) All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions
.
Two questions from each module with choice to answer
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BT 09 L 04
Teaching Scheme:
METABOLIC ENGINEERING
Credits: 4
3 hours lecture and 1 hour tutorials per week Objectives:
To understand how to regulate the metabolic pathways of bioconversion To understand the catabolite regulation pathways and also the primary metabolite synthesis pathways
Module - I Review of cellular metabolism (Transport processes, fuelling reactions, biosynthesis, growth energetic) Review of cellular stoichiometry. Regulation of metabolic pathways: Levels of regulation of enzymatic activity (overview of kinetics, reversible and irreversible inhibitions, allosteric enzymes and cooperativity) regulation of enzymes concentration (Control of transcription and translation example with respect of lacoperon and catabolite repression)- Global control- regulation of metabolic networks (Branch point classification, coupled reactions and global currency metabolities and energy regulation) Module - II Metabolic engineering in practice: Concept of directed cellular energy utilization analytical and synthetic elements of metabolic engineering targets of metabolic engineering. Metabolic Pathway analysis (Typical case study: Lysine Biosynthesis) Strategies for redirecting branched and linear pathways: (Alteration of feed back regulation; limiting accumulation of end product feed back resistant mutants, alteration of permeability). Module - III Metabolic Flux Analysis: Concept and utility of MFA Theory case studies over determined systems experimental determination of MFA by isotope labeling applications of MFA: Case studies- concept & fundamentals of metabolic control analysis (Basic concept only). Module - IV Application of pathway manipulations: Strategies for overproduction of primary metabolites. Strategies for overproduction of secondary metabolites (precursor effects, prophophase idiophase relationship, enzyme induction, feed back regulation.) Bioconversions: (ME concepts applied in process decisions for enhanced bioconversion). Examples of pathway manipulations: Enhancement of product yield (alcohol, amino acids) extension of substrate ranges (lignocelluloses utilization) extension of product spectrum (antibiotic, biopolymers) - improvement of cellular properties (alteration of metabolism, enhanced efficiency and yield, genetic stability).
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Text Books/References
1
2. 3
G Stephanopoulos et al; Metabolic Engineering principles & Methodologies T. Scheper R Faurie, J. ThommelAdvance in Biochemical engineering Biotechnology: Microbila production of L Aminoacid Jens Hoiriis Nielsen, Sabine Arnold: Biotechnology for the future.
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BT 09 L 05
Teaching Scheme:
GENOMICS AND PROTEOMICS
Credits: 4
To provide an advanced knowledge of gene expression and gene therapy To understand the various technologies of gene mapping, proteomic techniques and new target identification for drug discovery
Module I Principles of gene expression- Genome Mapping Human Genome Project Genomes of other organisms Role of genomics in drug discovery and development peptide nucleic acid technology. Module - II Genomics in Biopharmaceutical Industry Functional Genomes Pharmacogenetics Genomics in relation to molecular diagnosis
Module - III Molecular therapeutic technologies Gene Therapy new targets for drug discovery Module - IV Proteomics: Proteomic Techniques Pharmaceutical Applications Proteomics in drug discovery in human. Role of animal models in identification of genes for disorders knockout mice.
References S. Sahai, Genomics and Proteomics, Functional and Computational Aspects, Plenum Publications.
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PART A: Short answer questions (one/two sentences) marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions marks Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions marks Two questions from each module with choice to answer
5 x 2 marks=10
4 x 5 marks=20
4 x 10 marks=40
BT09 607(P) MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB

Teaching Scheme: 3 hours practical per week Objectives: Credits: 2
To do experiments for study of DNA structures and RNA structures

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Isolation of genomic DNA from eukaryotic cells. Isolation of RNA from eukaryotic cells Isolation of proteins from eukaryotic cells Isolation of genomic DNA from prokaryotic cells Isolation of plasmid DNA from prokaryotic cells Restriction mapping of plasmid DNA Gel electrophoretic separation of DNA and molecular weight determination Gel electrophoretic separation of RNA Gel electrophoretic separation of proteins Transblot analysis of DNA Gel extraction of DNA PCR amplification of DNA
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13. 14. 15. 16. 17. 18. Conjugation in E.coli Transformation in E.coli (Preparation of competent cells) Induction of lac operon (expression of beta galactosidase and assay) Cloning of DNA into plasmid vector. Electroporation of DNA ELISA
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60% 30% 10% 50
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BT 09 608 (P)
Teaching Scheme: Three hours practical per week Objectives:
BIOINFORMATICS LAB
Credits: 2
To understand the fundamental principles of bioinformatics and using the knowledge to tackle various research problems
1. Unix commands 2. PERL programming 3. Biological databases i. Nucleotide sequence databases ii. Protein sequence databases iii. Protein structure databases. 4. Sequence Analysis blastn, blastp, blast2, fasta 5. Multiple sequence alignment and phylogenetic interpretation Clustal, Phylip, Phylodraw. 6. Gene prediction-Genscan, ORF finder, Genmark 7. Protein prediction-Conserved domain databases, Protparam, Signalp, Motif 8. Molecular visualization Rasmol, Cn3D, Swiss PDB Viewer 9. Structure prediction GOR, nnpredict, Swissmodel server 10. Structure alignment and docking Calpha match, VAST, SAT, Hex 11. Primer design-Primer3.
Sessional work assessments Lab Practical and Record Ttest/s Regularity Total marks
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Semester End Examination pattern Fair record Viva voce Procedure and tabulation form, Conducting experiments and results Total marks
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SEVENTH SEMESTER BT 09 701

BIOPROCESS PLANT DESIGN
To impart the basic concepts of mechanical and process design of process plants To impart design principles for bioreactor design
Module - I Introduction. General design information for chemical process plants and bioprocesses. Development of flow sheet. Piping and instrumentation diagram and its description. Mechanical design of process equipment. Introduction to the design of cylindrical and spherical vessels for internal and external pressures. Design of heads, closures and supports. Design of tall vessels. Pipe line design. Materials of construction for process and bioprocess plants. Module - II Detailed process design of double pipe heat exchanger, shell and tube heat exchangers, distillation columns, rotary and tray dryers. Module III Design principles (no detailed design; design approach only) for the following: Stirred tank bioreactor, bubble column fermenter, airlift fermenter, fluidized bed bioreactor, photo bioreactor, packed column bioreactor, plug flow reactor. Fermenter power input calculations, sparger design Design of sterilizers batch and continuous (Use of chemical Engineers Handbook (both 7th and 8th edition) by Perry& Chilton and relevant attested copies of datas are permitted in the examination hall.)
Text Books 1. Perry & Chilton (Ed) Chemical Engineers Handbook (7th and 8th edn.) 2. Peters and Timmerhaus: Plant design and Economics for Chemical Engineers 3. Joshi, M.V Process Equipment design. 4. S.B Thakore, B.I Bhatt, Introduction to Process Engineering and Design, The McGraw Hill Companies. 5. Michael L Schuler & Fikret Kargi Bioprocess Engineering Prentice Hall of India Pvt Ltd. 6. Pauline M Doran Bioprocess Engineering Principles Academic Press.
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BT 09 702
Teaching Scheme:
DOWNSTREAM PROCESSING
Credit: 4
To impart knowledge of downstream processing operations To attain knowledge of the applications of the above operations in the bioprocess industries
Module I Role of downstream processing in biotechnology Role and importance of downstream processing in biotechnological processes Problems and requirements of bioproduct purification.
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Economics of downstream processing in Biotechnology cost cutting strategies characteristics of biotechnological mixtures process design criteria for various classes of bioproducts (high volume , low value products and low volume, high value products)physicochemical basis of bio separation processes. Module - II Primary separation and recovery process:Cell disruption methods for intracellular products(sonication, beadmills, homogenizers etc) removal of insolubles biomass ( and particulate debris) separation techniques- flocculation and sedimentation centrifugation and filtration methods. Chemical and enzymatic lysis. Module - III Enrichment operations: Membrane based separations micro and ultra filtration theory- design and configuration of membrane separation equipment applications .Reverse osmosis Precipitation methods (with salts, organic solvents and polymers, extractive separations, aqueous two phase extraction, supercritical extraction In situ product removal integrated bioprocessing. Module - IV Product Resolution /Fractionations :Chromatographics techniques: Principles of Adsorption chromatography. Affinity chromatographic separation process, GC, HPLC, FPLC, Process configurations (packed bed, simulated moving beds) Hybrid separation technologies (membrane chromatography, electro chromatography etc) Product polishing: Gel Permeation chromatography, dialysis, crystallization, pervaporation Case studies for downstream processing of Industrial Bioproducts High Volume/Low Value Products (Citric acid/Penicillin) and Low Volume/High value Products (Recombinant proteins.)
Text books 1. Product recovery in Bioprocess Technology, BIOTOL, Series VCH, 1990. 2. P.A Belter E.L Cussler and Wei-Shou Hu, Bioseperations Downstream Processing for Biotechnology, Wiley Interscience Publication, 1988. 3. R.K Scopes, Berlin, protein Purification: Principles and Practices, Springer, 1982. References: 1. Wankat P.C, rate controlled separations, Elsevier, 1990. 2. Asenjo J.M Seperation processes in Biotechnology, 1993, Marcel Dekkere Inc 3. Bioseperations by Siva Shankar PHI Publications.
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BT09 703
Teaching Scheme:
ENVIRONMENTAL ENGINEERING
Credits: 3
2 hours lecture and 1 hour tutorial Objectives:
To impart basic concepts of air pollution, water pollution and pollution due to solid waste To impart knowledge about the design of equipment for controlling air and water pollution and pollution due to solid waste To study the waste treatment of major industries
Module - I Sources and classification of waste water. Physical, chemical and biological characteristics of waste water. Waste water sampling and analysis. Waste water microbiology
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Air pollution. Sampling and analysis of air pollutants. Air pollution control methods and equipment. Setting chambers, cyclone separators, fabric filters, electrostatic precipitator, wet scrubber. Control of gaseous emission absorption and adsorption. Noise pollution monitoring and control methods. Module - II Wastewater treatment methods. Preliminary treatment, primary treatment. Secondary treatment. Design of activated sludge process. Aeration of activated sludge. Tricking filter, biotower, rotating biological contactor. Aerobic fluidized bed bioreactor. Chemical precipitation, coagulation, sedimentation. Design of clarifier. Disinfection. chlorination and ozonation, ultra violet light, activated carbon adsorption, Membrane method of wastewater treatment. Sewage treatment and disposal. Module - III Anaerobic digestion. Slow rate and high rate biomethanation. Anaerobic filter. Anaerobic contact process. Anaerobic fluidized bed bioreactor. Design of upflow Anaerobic Sludge Blanket (UASB) process. Sludge treatment and disposal Solid waste treatment composting (aerobic, anaerobic and vermi). Sanitary landfill. Incineration. Design of an incinerator. Recovery and recycling. Soil bioremediation. Module IV Wastewater treatment for industrial waste. Treatment methods for effluents from pulp and paper mill, dairy, distillery, tannery, food and allied industries, Edible oil refinery, soap and detergent industry, textile mill, cane sugar industry, rubber industry, drugs and pharmaceutical industry. slaughter house and meat processing industry. Common Effluent treatment Plants. Biomedical waste management. Hazardous waste management. Text Books/References 1. Met calf & Eddy Waste water Engg, disposal & Reuse McGraw Hill 2. Peavy Environmental Engg McGraw Hill 3. Rao M.N Air Pollution Tata McGraw Hill 4. S.P Mahajan Pollution control in Process Industries Tata MGgraw Hill Publishing Company.
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5 x 2 marks=10
4 x 5 marks=20
4 x 10 marks=40
BT 09 704
Teaching Scheme:
IMMUNOLOGY AND IMMUNOTECHNOLOGY

credits: 3
To impart knowledge on the immune system To impart knowledge on immunity to infection and molecular immunology
Module - I The Immune System: Introduction hematopoiesis and blood cell formation cells of the immune system lymphocytes their origin and differentiation antigens their structures and classification- complement and their biological functions. Module - II Humoral immunity: Structure and function of immunoglobulin Immunoglobulin classes and subclasses- genetic control of antibody production. B-Iymphocytes, their generation, activation and differentiation. Cellular immunology Major histocompatibility complex, Antigen processing and presentation-Tcell receptor-Tcell maturation, activation and differentiation, cytokines and the role in immune response. Module - III Immunity to infection: Hypersensitivity reactions Gell and Coombs classification IgE mediated hypersensitivity- antibody meditated hypersensitivity- immune complex meditated
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hypersensitivity delayed type hypersensitivity. Transplantation: Graft rejection evidence and mechanisms of graft rejection immunosuppressive drugs HLA and disease Auto immunity: Auto antibodies in humans-pathogenic mechanisms-experimental models of autoimmune diseases-treatment of autoimmune disorder. Module - IV Molecular Immunology: Preparation of vaccines application of recombinant DNA technology for the study of the immune systems-catalytic antibodies-immunotherapy with genetically engineered antibodies. Current topics in Immunology: Hybridoma technique and monoclonal antibody production Diagnotics methods: Immunodiffussion, immunoelectrophoresis. Radioimmunoassay ELISA, Western blot.
Reference Books 1. Janis Kuby, Immunology, W.H Freeman & Company. 2. Roitt I.M., Brostoff J and Male D.K Immunology Mosby Publication 3. Ivan I., Immunological Methods manual, academic Press.
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BT 09 LXX ELECTIVE II
Teaching Scheme: 3hours lecture and 1 hour tutorial per week Credits: 4
Any one from BT 09 L06 to BT09 L25 or Global Electives listed at the last with maximum of one global elective for one semester
BT 09 LXX ELECTIVE III
Teaching Scheme: 3 hours lecture and 1 hour tutorial per week Credits: 4
BT 09 707 (P) DOWNSTREAM PROCESSING LAB

Teaching Scheme: 3hours practical per week Objectives: Credits: 2
To conduct experiments in downstream processing operations

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Cell disruption techniques Filtration Centrifugation Sedimentation Leaching Membrane based filtration Ultra Filtration and Micro Filtration Protein precipitation methods and its recovery Two-phase aqueous extraction Liquid chromatographic techniques Electrophoretic separation techniques Dialysis Crystallisation Drying
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BT 09 708 (P)
Teaching Scheme: 3 Hrs practical per week Objectives:
REACTION ENGINEERING AND PROCESS CONTROL LAB

Credits: 2
To conduct experiments in reaction engineering and process control

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Kinetics of hydrolysis of esters Determination of activation energy Batch reactor Stirred tank reactor Plug flow reactor Fixed bed reactor Fluidized bed reactor Recycle bed reactor UV photo reactor RTD in CSTR Time constant of manometer Calibration of thermo couple Dynamics of liquid level systems-interacting and non-interacting Measurement of level by capacitance method Characteristics of P.I.D controller Control valve characteristics
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Semester End examination Fair record Viva voce Procedure and tabulation form, Conducting experiments and results
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BT 09 709 (P)
PROJECT
1 hour practical per week Objectives
Credit:1
To judge the capacity of the students in converting the theoretical knowledge into practical systems/investigative analysis.
Project work is for duration of two semesters and is expected to be completed in the eighth semester. Each student group consisting of not more than five members is expected to design and develop a complete system or make an investigative analysis of a technical problem in the relevant area. Project evaluation committee consisting of the guide and three/four faculty members will perform the screening and evaluation of the projects. Each project group should submit project synopsis within three weeks from the start of the seventh semester. Project evaluation committee shall study the feasibility of each project work before giving consent. Literature survey is to be completed in the seventh semester
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. Students should execute the project work using the facilities of the institute. However, external projects can be taken up in reputed industries, if that work solves a technical problem of the external firm. Prior sanction should be obtained from the head of the department before taking up external project work and there must be an internal guide for such projects. The objective of the project is to test the ability of the student to coordinate the entire knowledge of biotechnology engineering and to judge the students capacity in the design of plant/ process system. The project can be experimental or design based. The students are required to prepare the project report on a complete process showing the selection of alternatives, preparation of flow sheet, bioprocess calculations and detailed design calculations of the major items of equipments. The project should include mechanical design, capital cost; product cost estimation, profitability, breakeven analysis, plant location and lay out. The project selected should be an industrial problem. Any laboratory experimental data generated may be used for the design of the industrial plant. The assessment shall be based on individual and group performance. Each student has to submit an interim report of the project at the end of the 7th semester. Members of the group will present the project details and progress of the project before the committee at the end of the 7th semester. 50% of the marks is to be awarded by the guide and 50% by the evaluation committee.
InternalContinuous Assessment
20%- Technical relevance of the project 40%- Literature survey and data collection 20%- Progress of the project and presentation 10%- Report 10%- Regularity in the class
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EIGHTH SEMESTER BT 09 801

PLANT OPERATIONS, SAFETY AND BIOSAFETY
To impart the basic concepts of safety procedures carried out in chemical process plants and bioprocess plants To impart knowledge on safety and biosafety guidelines
Module - I Operational Parameters in a process plant, duties and responsibilities of operator, supervisor and process engineer and manager in a process plant. Raw materials scheduling, start-up, shut down. Common operational problems in process plants,Trouble shooting methods. The role of preventive maintenance and break-down maintenance. Plant utilities water, power, steam, air and fuels. Elementary aspects of quality management systems, Environment management systems-benefits of EMS certification, Requirements of EMS-Environmental policy, planning, implementation and operation, checking and corrective action management review. Occupational Health and safety Management Systems:- BS 8800, OHSAS 18001 & 18002, ISO 9000. Good Manufacturing Practice in industry. Module - II Chemical hazards, Toxic chemicals-dusts, gases, fumes, mists, vapours and smoke. Exposure evaluation. The concept of threshold limit, chronic and acute exposure effects. Safety equipments in chemical plants working principles. Safety in chemical reactions and storage and explosive or flammable dust, gases, vapours etc. Identification of hazards. Chemistry of fire, composition of combustion flame, heat, fire, gases, smoke, ignition temperature, LFL UFL-Flash point, Fire point. Spontaneous combustion. Classification of fires, flammability principles. Fire prevention, Fire protection in process plants. Fire and Explosion rating of process plants- Introduction to the modeling of fire explosion and toxic gas dispersion, pool fire, torch, BLEVE, HAZOP and HAZAN. Event probability and failure frequency analysis (Fault and Event Tree analysis). Designing for safety, emergency planning and disaster management.
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Module - III Biosafety guidelines and regulations, FAO, USDA & DBT guidelines on biosafety. Containment of equipment and apparatus in biotechnology industry and research, Good laboratory Practices. Biosafety levels- Containment in BSL-1, BSL -2, BSL-3, BSL-4 levels, design requirements and standard microbiological laboratory practices in each level. Design for Good Laboratory Practices, Waste disposal, shipping transportation and treatment of bio-hazardous materials and waste products. Decontamination of industrial and laboratory wastes:- agents, selection and methods for decontamination. Module IV Hazards of genetic engineering, bio-safety for human health and environment, social and ethical issues pertaining to genetic engineering, bio-safety in relation to transgenic research, rDNA guidelines and applications. Bio-safety and cartagene protocol, Environmental monitoring of GM crops and organisms. Risk assessment of GM organisms and crops released into the environment. References: 1. F.P Lease: Loss prevention in Process plants worth, London 2. G.L Wells: Safety in Process Plant Design, IChem E/ Godwin 3. Comprehensive Bio technology Vol IV, Murray Moov Young. 4. D.A Shapton and R.G Board: Safety in microbiology, Academic Press London.
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BT 09 802
Teaching Scheme:
INDUSTRIAL BIOTECHNOLOGY AND BIO PHARMACEUTICALS
2 Hrs lecture and 1 hour tutorial per week Objectives:
Credits: 3
To describe the various technologies involved in manufacture of industrial products in biotechnology To give knowledge about the manufacture of major biopharmaceuticals
Module I A review of industrial fermentation and enzymatic processes and products. Role of a bioprocess engineer in bioprocess industry. Outline of the various unit operations involved in the upstream and downstream operation of a bioprocess plant. Process flow sheeting. A survey of organisms. Isolation and improvement of microbial strains, mutation and mutant selection. Recombination protoplast fusion, recombinant DNA technology for overproduction of primary metabolites and secondary metabolites, general fermentation process economics and costing of products. Good manufacturing practice. Module - II Production of citric acid, gluconic acid, lactic acid, acetic acid, ethanol, acetone/butanol, glutamic acid, lysine, pencillins, cephalosporin, tetracyclins, griseofulvin, bakers yeast, alcoholic beverages, high-fructose corn syrup. Module - III Vitamins B12, riboflavin, protease, amylase, glucoamylase, glucose isomerase, rennet, catalase, lipase, xanthan gum, dextran, PHA, PHB, aspartame, nisin, SCP. Module - IV Insulin, Interferon, erythropoietin, streptokinase, lymphokinase, interleukin, blood factor VIII, monoclonal antibodies, vaccines.
Text Books/references 1. Gary Walsh Biopharmaceuticals: Biochemistry and biotechnology John Willey & Sons Ltd 2. L.E Casida Industrial Microbiology New Age International Publishers 3. S.N Jogdand Bio pharmaceuticals Himalaya Publishing House. 4. Mooray Muyoung Comprehensive Bio technology Pergames press. 5. Prescott and Dunn: Industrial Microbiology CBS Publishers.
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5 x 2 marks=10
4 x 5 marks=20
4 x 10 marks=40
BT 09 LXX ELECTIVE IV
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BT 09 LXX ELECTIVE V
BT 09 805 (P)
Teaching scheme: 3 hours per week Objective :
SEMINAR
Credits: 2
To assess the ability of the student to study and present a seminar on a topic of current relevance in biotechnology engineering and allied areas.
It enables the students to gain knowledge in any of the technically relevant current topics and acquire the confidence in presenting the topic. The student will undertake a detailed study on the chosen topic under the supervision of a faculty member, by referring papers published in reputed journals and conferences. Each student has to submit a seminar report, based on these papers; the report must not be reproduction of any original paper. A committee consisting of three/four faculty members will evaluate the seminar.
Internal continuous assessment 20% - Relevance of the topic and literature survey 50% - Presentation and discussion 20% - Report 10% - Regularity in the class and participation in the seminar
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BT 09 806 (P) PROJECT

Teaching scheme 11 hours practical per week Total Credits: 7 Credits for interim evaluation: 2 Credits for final evaluation: 5
This project work is the continuation of the project initiated in seventh semester. The performance of the students in the project work shall be assessed on a continuous basis by the project evaluation committee through progress seminars and demonstrations conducted during the semester. Each project group should maintain a log book of activities of the project. It should have entries related to the work done, problems faced, solution evolved etc.
There shall be at least an interim evaluation and a final evaluation of the project in the 8th semester. Each project group has to submit an interim report in the prescribed format for the interim evaluation. Each project group should complete the project work in the 8th semester. Each student is expected to prepare a report in the prescribed format, based on the project work. Members of the group will present the relevance, design, implementation, and results of the project before the project evaluation committee comprising of the guide, and three/four faculty members specialised in biotechnology engineering and allied areas. 50% of the marks is to be awarded by the guide and 50% by the evaluation committee.
Internal continuous assessment 40% -Design and development 30% -Presentation and demonstration of results 20 % -Report 10 %- Regularity in the class
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BT 09 807 (P)
VIVA VOCE
Credits: 4
Objective :
To examine the knowledge acquired by the student during the B.Tech. course, through an oral examination
The students shall prepare for the oral examination based on the theory and laboratory subjects studied in the B.Tech. Course, , seminar, and project. There is only university examination for viva-voce. University will appoint two external examiners and an internal examiner for viva-voce. These examiners shall be senior faculty members having minimum five years teaching experience at engineering degree level. For final viva-voce, candidates should produce certified reports of, seminar, and project (two interim reports and main report). If he/she has undergone industrial training/industrial visit/educational tour or presented a paper in any conference, the certified report/technical paper shall also be brought for the viva-voce.
Allotment of marks for viva-voce shall be as given below.
Assessment in viva-voce 40 %- Subject 30% - Project 20%- Seminar 10%- Industrial training/ Industrial visit/ Papers presented at national level Maximum marks : 100
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ELECTIVES
For Elective II, III, IV and V in the seventh and eighth semesters.
BT 09 L06
Teaching Scheme:
GENE AND STEM CELL THERAPY

Credits :4
3hours lecture and 1 hour tutorial per week Objectives:
To impart knowledge on the transfer and expression of genetic materials To obtain a basic knowledge of the treatment of diseases using gene and stem cell therapy Prerequisite: No prerequisite
Module I Stem cell :Introduction stem-cells-properties and importance of stem cells sources of stem cells- adult, embryonic, cord blood stem cells-classification of stem cells-multi-potent, pluripotent, toti-potent, uni-potent stem cells, clinical application of stem cells. Module - II Stem cell therapy :Overview of stem cell therapy -harvesting of stem-embryonic, fetal and adult stemcell therapy- therapeutic cloning-current and potential stem cell treatments. Module - III Gene therapy:Understanding gene therapy types of gene therapy germ line gene therapy and somatic gene therapy-vectors in gene therapy-viral and non viral methods-using stem cells for gene therapy-developments in gene therapy-clinical applications. Module - IV Ethics of gene and stem cell therapy : Stem cell research-stem cell problems-concerns about stem cells-immunological challenges for stem-controversy and safety of stem cells-problems and ethics of gene therapy. Reference Books 1. Stem cell biology and gene therapy: Edited by Peter J. Quesenberry, Gary S. Stein, Bernard Forget. 2. Stem cells by ariff bongso, Eng Hin Lee, Sydney (FRW) Brenner 3. Embryonic stem cells by Kursad Turksen 4. Cell therapy by George Morstyn, William Sheridan.
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BT 09 L07
Teaching Scheme:
MOLECULAR DIAGNOSTICS
Credits :4
3hours lecture and 1 hour tutorial per week Objective:
To obtain a clear concept of molecular diagnostic procedures for diagnosing illnesses in sick people
Prerequisite: No prerequisite Module - I Host pathogen interactions in disease process; Protective immune response in Bacterial, Viral and Parasitic diseases; Cancer; Inappropriate immune response; Disease pathology and clinical spectrum; Clinical diagnosis of diseases; Molecular Genetics of the host and the pathogen
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Module II Biochemical disorders; Immune, Genetic and Neurological disorders; Molecular techniques for analysis of these disorders; Assays for the Diagnosis of inherited diseases; Bioinformatics tools for molecular diagnosis.
Antibody based diagnosis; Monoclonal antibodies as diagnostic reagents; Production of monoclonal antibodies with potential for diagnosis; Diagnosis of bacterial, viral and parasitic diseases by using ELISA and Western blot. Module III Isolation of DNA; purification and analysis; DNA sequencing and diagnosis; PCR and Array based techniques in diagnosis; single nucleotide polymorphism and disease association; Two dimensional gene scanning. Module IV Isolation of proteins and other molecules associated with disease; Process and their profiling for diagnosis; 2Danalysis of such proteins by sequencing individual spots by Mass spectrometry; Protein Micro array; Present methods for diagnosis of Specific diseases like Tuberculosis, Malaria and AIDS; Ethics in Molecular Diagnosis.
Texts/References: Campbell, M.A and Heyer L.J., Discovering Genomics, Proteomics and Bioinformatics, 2nd Edition, CSHL Press, Pearson/Benzamin Cummings San Francisco, USA, 2007. Andrew Read and Dian Donnai, New clinical Genetics, Scion Publishing Ltd, Oxfordshire, UK, 2007. James W Goding, Monoclonal antibodies: Principles and Practice, 3rd Edition, Academic Press, 1996.
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BT 09 L08
Teaching Scheme:
MOLECULAR PATHOGENESIS
Credits: 4
To impart the basic ideas of molecular methods used in pathology for noting the causes of diseases To understand the paradigms and future challenges in molecular pathogenesis
Prerequisite: No prerequisite Module I Introduction to pathogenesis, attributes of microbial pathogenicity, components of microbial pathogenicity. Population genetics of Microbial pathogenesis, methods to detect genetic diversity and structure in nature population, epidemiology, cryptic diseases.
Module II Host defences against pathogens, clinical importance of understanding of host defences, components of the host surface defences systems like mucosa and the defences systems of the eye, mouth, respiratory tract etc., components of the systemic defence like the tissue and blood.
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Module III Virulence and virulence factors, colonizing virulence factors, virulence factors damaging the host tissues, virulence genes and regulation of the virulence genes. Experimental methods to study host pathogen interaction, selecting the pathogen model, measurement of virulence, identification of potential virulence factors, modulation of immune response by vaccines, properties of vaccines, other immuno modulators. Module -IV Paradigms of Pathogenesis:Diphteria disease by colonization Disease without colonization, C.Botulinum and Staph aureus Cholera Intestinal infections, Shigella and E.coli infections Salmonella infections Fungal infections. Future Challenges: a. Gastric and duodenal ulcers-are they due to infections? Lyme disease and Syphills unsolved mystery. Legionnaires disease-aftermath of comforts b. Tuberculosis and other mycobacterial infection-re-emerging with vengeance. Rheumatie fever and glomerulo nephrits still a question to be solved. References 1. Iglewski B.H and Clark V.L Molecular basis of Bacterial Pathogenesis. 2. Talaro K and Talaro A. Foundation in Microbiology. W.C Brown Publishers, 1993. 3. Roitt K. and Talaro A. Foundations in Microbiology. W.C Brown Publishers, 1993. 4. Roitt 1, Essentials of Immunology, 8th edition. Blackwell Scientific Publishers, 1994. 5. Austyn.J.M and wood K.J Principles of Cellular and Molecular immunology. Oxford University Press 1993
Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class University Examination Pattern
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BT 09 L09
Teaching Scheme:
MEMBRANE SEPARATION TECHNOLOGY

Credits: 4
To impart knowledge on the uses of different membranes for separation procedures To study membrane separation techniques Prerequisite: No prerequisite
Module I Introduction: Separation process, introduction to membrane processes definition of a membrane, classifications membrane processes. Preparation of synthetic membranes: Types of membrane materials, preparation of synthetic membrane, phase inversion membranes, preparation technique for immersion precipitation, and preparation technique for composite membranes. Module II Characterization of membranes: introduction, membrane characterization. Characterization of porous membranes, characterization of non-porous membranes. Transport in membranes: introduction, driving forces, non equilibrium thermodynamics, transport through porous, non-porous and ion exchange membranes. Module-III Membrane Processes: Introduction, osmosis, pressure driven membrane processes. , Micro filtration, membranes for micro filtration, industrial applications, Ultra filtration, membranes for ultra filtration, industrial applications, Reverse osmosis and Nano filtration: membranes for reverse osmosis and nanofiltration, industrial applications, Electrically Driven processes. Introduction, electro dialysis, process parameters, membranes for electro dialysis, applications, Membrane electrolysis, Biopolar membranes, Fuel Cells. Concentration driven membrane processes: gas separation in porous and non porous membranes, membranes for gas separation, applications, pervaporation, membranes for pervaporation, applications, dialysis, membranes for dialysis applications, liquid membranes: aspects, liquid membrane development, choice of the organic solvent and carrier, applications, introduction to membrane reactors. Module IV Polarization phenomenon and fouling: introduction to concentration polarization, turbulence promoters, pressure drop, gel layer model, osmotic pressure model, boundary layer resistance model, concentration polarization in diffusive membrane separators and electro dialysis, membrane fouling, method to reduce fouling, compaction. Module and process design: Introduction, plate and frame module spiral wound module, tabular module, capillary module, hollow fiber module, comparison of module configurations.
References
119 1. S.P.Nunes , K.V, Peinemann, Membrane Technology in the chemical industry Wiley-VCH 2. Rautanbach and R.Abrecht, membrane Process, John Wiley & Sons. 3. J.G.Crespo, K.W.Bodekes, Membrane Processes in separation and Purification, Kuwer
Academic Publications. 4. Transport processes and Unit Operations by C.J.Geankoplis.
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BT 09 L10
Teaching Scheme:
RECOMBINANT DNA TECHNOLOGY

Credits :4
To impart the basic concepts of recombinant DNA technology To study the application of recombinant DNA technology
Prerequisite: No prerequisite
Module I Introduction of recombinant DNA into a host (Bacteria, plants & animals by various methods) Transformation in vitro packaging into phages transfection reporter genes and gene targeting. Recombinant selection and Screening use of probes RNA, DNA and DNA nucleic acid hybridization southern, northern blotting colony and plaque hybridization Screening by immunochemical methods. Module II Salient features of prokaryotic and eukaryotic expression systems fusion proteins secreted proteins in vitro gene expression. Polymerase chain reaction (PCR) basic reactions inverse PCR RT PCR-RACE applications of PCR. Mutagenesis deletion mutagenesis oligonucleotide directed mutagenesis PCR based mutagenesis Site directed mutagenesis and its applications. Module III Chemical method of Maxam & Gilbert enzymatic (dideoxy chain termination) method of Sanger automated sequencing. Restriction mapping DNA fingerprinting chromosome walking chromosome jumping. Module IV Safety aspects of recombinant DNA technology. Diagnostics pathogenesis genetic diversity RELP analysis and DNA finger printing. Therapeutic proteins, novel proteins, vaccine, antibodies, herbicide resistance, insecticides. CaPO4 coprecipitation electroporation lipofection microinjection. Transgenic mouse transgenic fish Antisense technology and applications. References 1. Watson.J.D GTliman, N, Recombinant DNA. Scientific American Books, W.H.Freeman and Co.New York. 2. Bemur, R.Pastmek.J.J, Molecular Biology Principles and Applications in Recombinant DNA, Panima Publishing Cooperation, New Delhi.
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Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc 10% - Regularity In the Class.
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BT 09 L11
Teaching Scheme:
MICRO ARRAY TECHNOLOGY

Credits: 4
To impart a basic knowledge on gene chips To give ideas on the use of gene chips to diagnose diseases
Prerequisite: No prerequisite Module-1 Introduction to Genetics and Molecular Biology. Affymetrix Microarray Technologies, cDNA,
Module II Data Acquisition, Normalization and Outlier Detection; dChip, Affymetrix Microarray Suite, RNA Significance testing and Gene filtering. Threshold methods, t-test, fold change SAM, false positive rate, false discovery rate. Module III Cluster Analysis and Graphical tools: hierarchical clustering, k-means clustering, PAM. Prediction methods: class prediction, k-nearest neighbor, linear discriminant analysis, gene voting, random forest. Module IV Multivariate statistical techniques: Principal components, multidimensional scaling. Gene Co-Expression Network Analysis and Systems Biology.
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BT 09 L12
Teaching Scheme: 3hours lecture and 1 hour tutorial per week Objectives:
CANCER BIOLOGY
Credits :4
To study the principles of carcinogenesis To study the various treatments of cancer
Prerequisite: No prerequisite Module I Fundamentals of Cancer Biology:Regulation of Cell cycle, mutations that cause changes in signal molecules, effects on receptor, signal switches, tumour suppressor genes, modulation of cell cycle in cancer. Different forms of cancers, Diet and cancer.
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Module II Principles of Carcinogenesis:Chemical Carcinogenesis, Metabolism of Carcinogenesis, Principles of Physical Carcinogenesis, X-Ray radiation mechanisms of radiation Carcinogenesis. Module III Principles of Molecular Cell Biology of Cancer : Oncogenes, Identification of Oncogenes, Retroviruses and Oncogenes, detection of Oncogenes. Oncogenes/Proto Oncogene activity. Growth factors related to transformation. Clinical significances of invasion, heterogeneity of metastatic phenotype. Metastatic cascade. Basement Membrane disruption. Three step theory of Invasion, Proteinases and tumour cell invasion. Module - IV New Molecules for Cancer Therapy: Different forms of therapy, Chemotherapy, Radiation. Therapy, Detection of Cancers, Prediction of aggressiveness of cancer, advances in cancer detection. References 1. Maly B.W.J Virology a practical approach. IRL Press. Oxford, 1987. 2. Dunmock N.J.and Primrose S.B., Introduction to Modern Virology. Blackwell Scientific Publications, Oxford, 1988. 3. An Introduction top Cellular and Molecular Biology of Cancer, Oxford Medical Publications., 1991.
Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class niversity Examination Pattern
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BT 09 L13
Teaching Scheme:
STRUCTURAL BIOLOGY
Credits :4
To study the structures of biomolecules and their interactions To impart knowledge on the study of biomolecular structures and their functions
Prerequisite: No prerequisite Module -I Introduction : Levels of structures in Biological macromolecules, the chiralitys of biomolecules, proteins, nucleic acids, carbohydrates and lipids, cofactors, vitamins and hormones. Conformational Analysis :Forces that determine protein and Nucleic acid structure, basic problems. Polypeptide chains; geometric, potential energy calculations, observed values for rotation angles, hydrogen bonding, hydrophobic interactions and water structures; ionic interactions, disulphide bonds. Module II Protein folding :Types of proteins and interactions that govern protein folding, protein structure. The protein globule and hydrophic interaction, organized folds, folding mechanisms, membrane proteins, helix-coil transitions. Bimolecular interactions: Molecular recognition, supramolecular interactions, Functional importance of Protein protein and protein-nucleic acid interactions. Specific and nonspecific DNA protein complexes. Module III Structural Analysis of Macromolecules : Prediction of protein structure; Sequence structure relationships, Nucleic acids; general characteristics of nucleic acid structure, geometric, glycoside bond rotational isomers backbone rotational isomers and ribose puckering forces stabilizing ordered forms, base pairing, base stacking; tertiary structure of nucleic acids. Kinetics of Ligand interactions:Biochemical Kinetics studies, uni-molecular reactions, simple bimolecular multiple intermediates, steady state kinetics, catalytic efficiency relaxation spectrometry, ribonuclease as an example.
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Module IV Techniques for the Study of Biological Structure & Function 1: Size and shape of micro molecules photons, chromophore, transition dipole moments, absorbance, and concentration. Circular dichroism ,molecular chirality and structural transitions of macromolecules, methods of direct visualization macromolecules as hydrodynamic particles macromolecular diffusion ultra centrifugation viscometry. Techniques for the Study of Biological Structure & Function II :X-ray crystallography: determination of molecular structures, X-ray fiber diffraction electron microscopy: neutron scattering light scattering, NMR spectroscopy. Text Book
1. Tinoco, I,Jr, Sauer.K.Wang, J.C & Puglisi, J.D (2001) Physical Chemistry Principles and
Applications in Biological sciences, 4th ed. Prentice Hall. References: 1. Introduction to Protein Architecture, by A.M.Leak 2. Introduction to Protein Structure, by Banden and Tooze.
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BT 09 L14
Teaching Scheme:
MOLECULAR MODELLING & DRUG DESIGN

Credits: 4
To impart knowledge on various molecular modeling structures To impart knowledge on analog and structure based drug design
Prerequisite: No prerequisite Module I Introduction to Molecular Modelling:Introduction to molecular modelling. Use of models. Areas of application Single molecule calculation, assemblies of molecules. Reaction of the molecules. Drawbacks of mechanical models as compared to graphical models. Co ordinate systems twomatrix, potential energy surface. Quantum Mechanics:Postulates of quantum mechanics, electric structure calculations, abinitio, semi-empirical and density functional theory calculations, molecular size versus accuracy. Approximate molecular orbital theories. Module II Empirical Force Field Models: Molecular Mechanisms, energy calculations, Bond stretch, angle bending, tensional term Electrostatic interaction Van der Waals interactions. Miscellaneous interaction. Molecular Dynamics: Introduction, molecular Dynamics using simple models. Dynamics with continuous potentials. Constant temperature and constant dynamics. Conformation searching. Systematic search applications to protein folding. Module III Comparative protein modeling :Modelling by Homology the alignment, construction of frame work, selecting variable regions, side chain placement and refinement, validation of protein models Ramchandran plot, threading and ab initio modelling. Analog based drug design:Introduction to QSAR, lead module linear and nonlinear modelled equations, biological activities, physicochemical parameter and molecular descriptions, molecular modeling in drug discovery. Module IV Structure based drug design : 3D pharmacophores, molecular docking, De Novo Ligand design, free energies and solvation, electrostatic and non electrostatic contribution to free energies.
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Further applications on the design of new molecules: 3D data base searching and virtual screening. Source of data, molecular similarity and similarity searching, combinatorial libraries generation and utility. Text Books 1. Principles and applications of modeling by Leach 2. Molecular modeling by the Hans Peter Heltie & Gerd Falkens, VCH. References 1. Chemical Applications of Molecular Modelling by Jonathan Goodman. 2. Computational Chemistry by Guy H, Grant & W Graham Richards, Oxford University.
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BT 09 L15
Teaching Scheme:
BIOSENSORS AND BIOINSTRUMENTATION

Credits: 4
3hours lecture and 1 hour tutorial per week Objective s:
To study the basic types of different instruments used for monitoring and controlling parameters in bioreactors To explain the working of the above instruments Prerequisite: No prerequisite
Module I Basic concept of biosensors, biomolecules used as sensors, devices used in biosensors, methods of preparation of biosensors, principles of bioelectronics involved in bioinstrumentation. Module II Unmediated and mediated enzyme electrodes; basic techniques-Enzyme immobilization, protective membranes, instrumentation, ferrocene based glucose sensor, ferrocene based cholesterol biosensor. Applications of enzyme biosensors. Module III Principles, construction of microbial biosensors, Immobilization of microbes, electrochemical devices; application of microbial sensors. Immunoelectrodes; basic concept. Alkaline phosphates labeled immunoassays, glucose oxidase in electrochemical immunoassays. Immunoassays using enzymatic amplification electrodes. Coupling of immunoassays with enzymatic recycling electrodes. Module IV Transducers: Optical transducers, fluorescence transducers, Acoustic transducer ,Acoustic wave device, Acoustic wave device sensors for studying bimolecular interactions.Consuctimetric and imedimetric devices. Polarizable and nonpolarizable electrodes,acoustic, plasmon resonance, holographic and micro engineered sensors for monitoring low molecular weight analytes, proteins, DNA and whole cells. Text Book 1. Scragg.A.H-Bioreactors in Biotechnology Edited .by Ellis Horwood Ltd. England 1991.
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BT 09 L16
Teaching Scheme:
MOLECULAR MEDICINE
Credits: 4
To impart knowledge on molecular medicine To know the techniques used for treatment of diseases
Prerequisite: No prerequisite Module I Basic biochemistry, molecular biology and genetics relevant to Molecular Medicine. Human genome: implications and applications. Single Nucleotide Polymorphism.
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Module II Gene therapy as a potential tool to cure human diseases Recombinant molecules in medicine. Transgenic and knock out animal models. Module III Stem cell research and its application in human health. Intellectual property right issues and ELSI (Evaluation of the Ethical, Legal and Social Implications program). Module IV Personalised medicine. Introduction to system biology, system medicine and translational medicine. References: 1. Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine. Ganten, Dettv; Ruckpaul, Klaus 2. Travelling Around the Human Genome: An in Situ investigation, Bertarand Jordan. 3. Principles of Molecular Medicine, J.Larry Jameson 4. Molecular Medicine: An Introductory Text, R.J.Trent 5. Molecular Medicine, Alan David Blair Malcolm 6. Molecular Medicine: Insight into the Cellular and Molecular Basis of Disease Published by johns Hopkins University Press. 7. Introduction to Molecular Medicine, Dennis W.Ross Pounds.
PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions 4 x 5 marks=20 marks Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions 4 x 10 marks=40 marks Two questions from each module with choice to answer
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BT 09 L17
Teaching Scheme:
HAZARDOUS WASTE MANAGEMENT

Credits :4
To study the different types of hazardous wastes found in industries and other types of environment To gain knowledge of the methods used for hazardous waste treatment
Prerequisite: No prerequisite Module I Classification of hazardous waste-Hazardous waste designation system-Hazardous Waste (Management and Handling) Rules-European and US and Indian Acts. Preparation of a waste inventory-procedure and considerations-specific and non specific sources hazardous waste numbers and codes. Module II Generator requirement-transporter requirements-treatment, storage requirements-ground water monitoring The hazard ranking system-prioritization of actions-contingency plans-liabilities
and
disposal
Module III Hazardous waste minimization-benefits-elements of effective waste minimization programmewaste audit-waste exchange-recycling Module IV Treatment technologies-Physical, Chemical and Biological Treatment-Management of specific recyclable hazardous waste like precious metals, lead acid batteries. Land disposal-land treatment-deep well injection-the secure land fill-construction. Reference 1. Davis, M.L and Cornwell, D.A. Introduction to Environmental Engineering, McGraw Hill. 2. Liu I (Ed), Environmental Engineers Handbook, Lewis publishers. Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
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BT 09 L18 ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY

To study the various analytical techniques used in biotechnology
Prerequisite: No prerequisite Module I Photometry and spectrophotometry: The Beer-Lambert Law, percentage transmittance and absorbance, photoelectric colorimeters; spectrophotometers-types, UV visible, IR, atomic absorption, NMR and mass spectrophotometers. Module II Chromatography: Partition chromatography-mobile and stationary phases-paper chromatography-solvent systems-development of Rf value-ascending and descending techniquestwo dimensional chromatography-thin layer chromatography. Column chromatographypreparation of columns-gradient elution-analysis of fraction and elution profiles-ion exchange chromatography-preparation and activation of ion exchange materials-affinity chromatography- separation of macromolecules-gas chromatography and high performance liquid chromatograph (HPLC).
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Module III Electrophoresis-paper and gel electrophoresis-immuno electrophoresis-enzyme linked immuno absorbent assay (ELISA)-isoelectric focusing-two dimensional electrophoresis-capillary electrophoresis. Dialysis-separating membranes-factors affecting dialysis-gelfiltrationultriafiltration-application of filtration techniques. Differential centrifugation-preparation of cellular organelle and other materials: disintegration of cells, density gradient centrifugation; analytical ultracentrifuge-determination of molecular weight. Module IV Radio isotope techniques-radioactive disintegration-radioactive isotopes used in biologydetection of radioactivity-Geiger counters- -labelling of biological material with radioactive isotope-scintillation counting-liquid scintillation counters-autoradiography. Text Books/Reference 1. T.G.Cooper:Tools of Biochemistry, : Analytical Biochemistry, Longman 2. D.Holme and H Peck-Analatical Biochemistry, Longman 3. Willard Merrit and Deana Settle: Instrumental Methods of Analysis, CBS Publishers & Distributors. Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
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BT 09 L19 ENERGY ENGINEERING

To impart the knowledge of energy sources and their harnessing technologies. To understand about energy audit and conservation in industries
Prerequisite: No prerequisite Module I Energy-units of energy-conservation factors-general classification of energy-world energy resources and energy consumption-Indian energy resources and energy consumptionenergy crisis-energy alternatives-electrical energy from conventional energy resources-internal combustion engines-steam turbines-gas turbines-hydro turbines (thermodynamic cycles not included)-nuclear reactors-thermal, hydel and nuclear power plants(process outlines only)efficiency, merits and demerits of the above power plants, combined cycle power plants-fluidized bed combustion-small hydropower. Module II Solar energy-solar thermal systems-flat plate collectors-focussing collectors-solar water heatingsolar cooling-solar distillation-solar refrigeration-solar dryers-solar pond-solar thermal power generation-solar photovoltaic systems-solar cells-solar photovoltaic power generation-solar energy application in India-energy plantations. Wind energy-types of wind mills-types of wind rotors-darrieus rotor and gravian rotor-wind electric power generation-wind power in Indiaeconomics of wind farm-ocean wave energy conversion-ocean thermal energy conversion-tidal energy conversion-geothermal energy conversion. Module III Biomass energy resources-thermochemical and biochemical methods of biomass conversion,combustion-gasification-pyrolysis-biogas production-ethanol-fuel cellalkaline fuel cell-phosphoric acid fuel cell-molten carbonate fuel cell-solid oxide fuel cell-solid polymer electrolyte fuel cell-magneto hydro dynamics-open cycle and closed cycle systems-magneto dynamic power generation-energy storage routes like thermal energy storage, chemical, mechanical, electrical storage. Module-IV Energy conservation in chemical process plants.- energy audit- energy saving in heat exchangers, distillation columns, dryers, ovens, furnaces and boilers- steam economy in chemical plants- energy conservation in petroleum, fertilizer and steel industriescogeneration, pinch technology- recycling for energy savingelectrical energy conservation in chemical plants, energy conservation in bioprocess plants- environmental aspects of energy use.
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Reference Books 1. Bansal N.K., Kleeman M.and Meliss M., Renewable energy sources and conversion tech., Tata McHraw H. 2. Pandey G.N.A Text book on energy systems and engineering, Vikas publishing house. 3. Rao, S, & Parulekar B.B, Energy Technology, Khanna publishers 4. Rai G.D., Non-conventional energy sources, Khanna publishers 5. Nagpal G.R., Power plant Engineering, Khanna publishers
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BT 09 L20 TRANSPORT PHENOMENA IN BIOPROCESS SYSTEMS

Teaching Scheme: 3 hours lecture and 1 hour tutorial per week Objectives: Credits :4
To impart the basic concepts of transport phenomena in process systems To develop a better understanding about momentum transfer, heat transfer and mass transfer
Prerequisite: No prerequisite Module I Momentum transfer: Momentum transfer in bioprocess, comparison with other transport processes, effect of flow properties in momentum transfer and oxygen mass transfer. Oxygen transport: Oxygen transport to microbial cultures-Gas liquid mass transfer fundamentals, oxygen requirement of microbial cultures. Oxygen requirements of microbial cultures oxygen mass transfer fundamentals. Oxygen transfer and oxygen demand. Module-II Oxygen transport: Oxygen transfer by aeration and agitation Determination of oxygen mass transfer coefficient by various methods including dynamic gassing out and oxygen balance methods. Momentum transport by agitation: Power requirements and mixing characteristics of ungassed and gassed systems. Concept of power number, use of monographs. Defining impeller Reynolds number for Newtonian and non-Newtonian fluids. Concept of aeration rate to calculate impeller power requirement of gassed systems. Module III Mixing: Mixing and bioreaction interactions-flow regimes with and without baffles, various types of impellers and mixing equipment. Scale up: Scale up criteria for mixing equipment. Application of mixing in bioprocessing. Module _IV Heat transfer I: Various modes of heat transfer. viz conduction, convection and radiation. Mechanism of heat transfer by conduction. Fouriers law .conductive heat transfer through a series of resistances. Heat transfer II: Analogy between heat, mass and momentum transfer. Application of heat transfer in bioprocesses.
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Text Books Introduction to Biochemical Engineering, D.G.Rao. Tata Mc Hill (2005) Bioprocess Engineering Principles Paul M.Doran. Academic press (1995) Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
BT 09 L 21 DESIGN AND ANALYSIS OF BIOREACTORS

Teaching Scheme: 3hours lecture and 1 hour tutorial per week Objectives: Credits: 4
To impart the basic concepts of different type of bioreactors used in bioprocesses
To gain knowledge about the design of such bioreactors

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Module I Ideal bioreactors Fed-batch reactor, Enzyme catalyzed reactions in CSTR, CSTR reactors with recycle and wall growth, ideal plug flow tubular reactor. Module II Reactors with non-ideal mixing. Mixing times in agitated tanks. Residence time distributions models for non ideal reactor. Mixing-bioreaction interactions. Module III Multiphase bioreactors. Conversion of heterogeneous substrates. Packed bed, bubblecolumn, fluidized bed and trickle bed reactors. Module IV Animal and plant cell reactor technology. Environmental requirements for animal cell cultivation. Reactors for large-scale production using animal cells. Plant cell cultivation.
References 1. Bailley & Ollis Biochemical Engg. Fundamentals McGraw Hill 2. Pauline M Doran Bioprocess Engineering Principles Academic Press.
Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class. University Examination Pattern
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BT 09 L22
Teaching Scheme:
MODELLING AND SIMULATION OF PROCESS PLANTS
Credits: 4
To impart the basic ideas on the modelling and simulation of process plans To represent processes in the form of mathematical models to simplify their design
Prerequisite: No prerequisite Module I Basic modelling principles-uses of mathematical modelling-classification of modelling techniques-fundamental laws-energy equations-continuity equation-equations of motion-transport equations-equations of state-equilibrium states and chemical kinetics-examples. Module II Mathematical models for chemical engineering systems-continuous flow tanks-enclosedenclosed vessel-mixing vessel-mixing vessel mixing with reaction-reversible reaction-steam jacketed vessel-boiling of single component liquid-open and closed vessel-continuous boiling system-batch distillation. Module III Gas flow systems-hydraulic transients between two reservoirs-reaction kinetics-general modelling scheme-liquid phase CSTR-batch reactor-ideal binary distillation column-distributed systems-jacketed tubular reactor-laminar flow in a pipe-counter current heat exchanger. Module IV Digital simulation-numerical integration-Euler and fourth order Runge Kutta methods- simulation of gravity flow tank CSTR in series-non isothermal CSTRbinary distillation column-batch reactor References 1. Luben W.L., Process Modelling Simulation and Control for Chemical Engineers McGraw Hill 2. Franks RGE Mathematical Modelling in chemical engg John Wiley 3. Biquette WB Process Dynamics- Modelling analysis with simulation
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BT 09 L23 NANOBIOTECHNOLOGY
Objective:
To impart basic ideas on nanoparticles To impart knowledge on the use of bionanoparticles and their applications in biotechnology
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Module I Introduction to Nanobiotechnology and Nanomedicine, Visualization and manipulation on nanoscale. Atomic Force Microscopy, Magnetic Resonance Force Microscopy, Scanning Probe Microscopy, Nanoscale Scanning Electron Microscopy, Optical Imaging with a Silver Superlens. Module II QuantumDots, Gold Nanoparticles, Lipoparticies, Assembly of Nanoparticles into Micelles, Biomedical applications of self-assembly of nanoparticles, Paramagnetic and superparamagnetic nanoparticles, Fluorescent nanoparticles. Module III Bacterial structure relevant to nanobiotechnology, Cubosomes, Dendrimers, DNANanoparticle Conjugates, DNA Octahedron, Fullerenes, Nanoshells, Carbon Nanotubes, Nanopores, Nano structured Sillicon. Module IV Molecular motors, Nanoparticles for molecular diagnostics, Nanobiosensors, Nanopharmaceuticals, Nanoparticle Based Drug Delivery, Nanostructures for Tissue Engineering/Regenerative. Medicine, Ethical safety, and regulatory issues of nanomedicine.
References 1. Nanobiotechnology: Bioinspired Devices and Materials of the Future: Oded Shoseyov and llan Levy. 2. Nanomaterials and Nanosystems for Biomedical Applications: M.Reza Mozafari. 3. The Handbook of Nanomedicine, Kewal K.Jain 4. Bio Nanotechnology, Elisabeth S.Pappazoglou, Aravind Parthasarathy 5. Biomedical Nanostructures, Kenneth E.Goonsalves, Craig R.Halberstadt, Cate T. Laurecin, Lakshmi S.Nair. Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
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BT 09 L24 BIOETHICS & INTELLECTUAL PROPERTY RIGHTS

To impart knowledge on bioethics and intellectual property rights To study the various ethical issues occurring in biotechnology
Prerequisite: No prerequisite Module I Biotechnology and Bioethics. Ethical biotechnology? (Rights, Confidentiality, Animal Rights, Environmental Ethics, Decision Making) Ethical Aspects of Designer Babies, genetic screening and prenatal testing issues of ethics in biomedicine. Transgenic plants. The debates of GM foods. Terminator technology, Ethical, issues of the Human Genome Project. Ethical issues in pharmaceutical drug research. Orphan drugs. Module II Intellectual Property Rights Development and need for IPR in knowledge based industries. Various types of intellectual Property Rights with examples (Trademarks, Copyrights, Industrial Designs, Patents, Geographical Indicators etc) Objectives of the patent system Basic principles and general requirements of Patents (Novelty, Utility Non obviousness. Etc) and tenets of patent law Product and Process Patents)
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Module III The patenting process in India Exercising and enforcing of Intellectual Property Rights. Rights of IPR owner Brief overview of patent filing in India. Criteria for patent infringement Various Amendments to Patent Law in India. Comparison of Patent Law in India and the US. International conventions and treaties: TRIPS. Evolution and present status. WIPO and its functioning. CBD Treaty. Paris and Berne Conventions Enforcement and Dispute Settlement in WTO Patent Cooperation Treaty IPR and WTO regime. Module IV Biotechnological inventions and patent law patentable subjects and protection in biotechnology. The patentability of microorganisms Diamond vs. Chakrabarty Case Bioprospecting & Biopiracy (Case studies of Neem / Turmeric / Arogyapacha of Kani Tribals in Kerala/Rosy Periwinkle of Madagascar)-Traditional knowledge Systems (TKS) Options for protection of Traditional Knowledge Systems. Need for Sui Generics Systems. TKS and the National and International Arena. Biodiversity and Farmers rights IPR and Plant Genetic Resources Plant Breeder Rights .UPOV Treaty. Text Books 1. Ethical Issues in Biotechnology. Edited by Richard Sherlock and John D.Morrey. 2002 Publishers Lanham, Md: Rowman and Littlefield. 2. J.Rehm and G.Reed, Biotechnology, Second Edition, Multi Volume Treatise, Volume 12 Legal Economic and Ethical Dimensions, VCHPublishers. 3. Prabuddha Ganguli Intellectual Property Rights-Unleashing the Knowledge Economy. Tata McGraw Hill Publishing Company Limited, New Delhi. 4. Beier, F.K, Crespi, R.S and Straus, T.Biotechnology and Patent protection Oxford and IBH Publishing Co. New Delhi. 5. Sasson A, Biotechnologies and Development, UNESCO Publications. 6. Jeffrey M.Gimble, Academia to Biotechnology, Elsevier, Academic Press. Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class University Examination Pattern
PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions 4 x 5 marks=20 marks Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions Two questions from each module with choice to answer 4 x 10 marks=40 marks
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BT 09 L25 BIOMATERIALS
To study the structure and characteristics of different types of biomaterials of natural and synthetic origin To give an idea on the effective uses of these materials
Prerequisite: No prerequisite Module I Structure of solids. Review of basic concepts. Biomaterials, definition, classification. Polymers, metals, alloys, ceramics and composites, physical, chemical and mechanical aspects of bulk and surface properties of metallic ,polymer and ceramic biomaterials (in vivo and in vitro ) Corrosion studies. Structure property relation. Characterisation of biomaterials. Bulk analysis-XRD, FTIR, SEM, TGA etc. Surface analysis-XPS, SIMS, AES, STM etc. Module II Hard tissue replacement implant: orthopaedic implants (hip, knee), dental implants, adhesives and sealants. Soft tissue replacement implant. Skin implant, burn (wound), dressings/ synthetic skin, dialysis membranes, scaffolds, vascular implants, heart valve implants. Artificial kidneys and livers. Sutures, biomaterials for gene delivery. Hydrogel as stimuli- sensitive biomaterials, ophthalmologic implants, biomaterials for drug delivery Module III Blood and tissue compatibility of biomaterials and their in vitro and in vivo assessment. Tissue response to biomaterials. Importance of interfacial tissue reaction ( eg. Ceramic bone tissue reaction ). Qualification of implant ( in vivo and in vitro ) Blood materials interaction. Mineralization and encrustation, microbialbiofilm formation, bacterial adhesion toxicology, degradation of biomaterials in biological environments. toxicity of biomaterials, acute and chronic toxicity studies. Implant associated infection Module IV Biopolymers, definition, plant and animal biopolymers- polynucleotide, polyamides, polysaccharides, polyisoprene, lignin, polyphosphate and poly hydroxyl alkanoates. Application and chemical synthesis of super absorbent polmers, polyethylene glycol, polypropylene glycol, poly tetra methylene glycol, polyglycerine. Bioplastics and environment, commercial bioplastics. Natural fibers like silk, wool, flax, jute, linen, cotton, sisal, bamboo. Biocomposite- properties and applications Text books/ references 1 Ratner, Hoffman, Schoen Biomaterial science- an introduction to materials in medicine Academic press 2 Park .J.B. Biomaterials- science and engineering, Plenum press 3 Sharma C.P., Szycher.M Blood compatible materials and devices Technomic publishing company 4. R. M. Johnson, R. M. Mwaikambo, Tucker Biopolymers Rapra technology.
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Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions 4 x 10 marks=40 marks
Two questions from each module with choice to answer
GLOBAL ELECTIVES
1. 2.
ME 09 L23 ME 09 L25 L23 L24 L25 L23 L24 L23 L25 L24
Industrial Safety Engineering Energy Engineering and Management Total Quality Management Industrial Psychology Entrepreneurship Simulation and Modeling Computer based Numerical Methods Nanomaterials and Nanotechnology Project Engineering Management Information Systems
3. PE 09 4. PE 09 5. PE 09 6. CS 09 7. CS 09 8 CH 09
9. CH 09 10. IT 09
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ME09 L23: Industrial Safety Engineering

Teaching scheme Credits: 4 3 hours lecture and I hour tutorial per week Objectives To provide on concept of safety in industry, principle of accident prevention, major hazards, consequences and concept of reliability. Pre-requisites: Nil Module I (14 Hours) Introduction to the concept of safety-Need-safety provisions in the factory Act-Laws related to the industrial safety-Measurement of safety performance, Safety Audit, Work permit system, injury and accidents-Definitions-Unsafe act unsafe condition- causes, investigations and prevention of accidents, hazards, type of industrial hazards-nature, causes and control measures, hazard identifications and control techniques-HAZOP, FMEA,FMECA etc. Module II (14 Hours) Concept of Industrial hygiene, programmes-Recognition Evaluation- Control, Noise- source effects and noise control, exposure limits standards, Hearing conservation programmes, Fire fire load-control and industrial fire protection systems, Fire Hydrant and extinguishers, Electrical Hazards, protection and interlock-Discharge rod and earthling device, safety in the use of portable tools. Module III (13 Hours) Logics of consequence analysis-Estimation-Toxic release and toxic effects-Threshold limit values, Emergency planning and preparedness, Air pollution-classification- Dispersion modeling -pollution source and effects- -control method and equipments-Gravitational settling chamberscyclone separators-Fabric filter systems-scrubbers etc. Module IV (13 Hours) Concept of reliability-Definition-Failure rate and Hazard function, System reliability modelsseries, parallel systems, reliability hazard function for distribution functions-exponential-normal lognormal-weibull and gamma distribution.
Text books 1. Thomas J. Anton, Occupational Safety and Health Management, McGraw Hill 2. Ian T.Cameron & Raghu Raman, Process Systems Risk Management, ELSEVIER Academic press. 3. C.S.Rao, Environmental Pollution Control Engineering, New Age International Limited 4. L. S. Srinath, Reliability Engineering, East west Press, New Delhi
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Reference books 1. Frank E. McErloy,P.E; C.S.P, Accident Prevention Manual for Industrial Operations,NSC Chicago. 2. Lees F.P, Loss Prevention in Process Industries, Butterworths, New Delhi. 3. BHEL,Occupational Safety Manual, Tiruchirappalli.
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ME09 L25: Energy Engineering and Management

Objectives To provide knowledge on energy conservation and management. To impart the basics of renewable energy technology Pre-requsites: Nil Module I (13 hours) Energy and environment: Introduction fossil fuel reserves world energy consumption green house effect global warming renewable energy sources environmental aspects utilization energy prices energy policies Module II (14 hours) Energy conservation: Industrial energy use energy surveying and auditing energy index energy cost energy conservation in engineering and process industry, in thermal systems, in buildings and non conventional energy resources schemes. Module III (14 hours) Energy technologies: Fluidized bed combustion fluidized bed boilers waste heat recovery systems heat pump and refrigerators wind energy collectors and storage systems insulated pipe work systems. Module IV (13 hours) Energy management: Energy management principles energy resources management energy management information systems computerized energy management. Costing techniques cost optimization optimal target investment schedule financial appraisal and profitability. Text Books 1. W. R. Murphy, G. Mc Kay, Energy Management, Butterworths, London Reference Books 1. O. Callaghn, Design and Management for energy conservation, Pergamon Press, Oxford 2. D. Merick, Energy - Present and Future Options, vol 1 and 2, John Wiley and Sons 3. N. A. Chaigier, Energy Consumption and Environment, McGraw Hill
150
PE09 L23: Total Quality Management

Objectives To impart knowledge on the concept of quality tools for analysing quality statistical tools in quality acceptance sampling life tests Module I (14 hours) Definition of quality-internal and external customers- vision statement mission statements objectives goals targets- evolution of TQM Defining TQM stages in TQ M implementation-TQM models Module II (14 hours) SWOT analysis-strategic planning-customer focus-quality function deployment-customer satisfaction measurement-seven new management tools-Deming wheel-zero defect concept-bench marking-six sigma concepts-failure mode and effect analysis-poke yoke
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Module III (13 hours) Five S for quality assurance-quality circle philosophy-failure rate analysis-mean failure rate-mean time to failure (MTTF)-Mean time between failure (MTBF)-hazard models-system reliabilityavailability- maintenance Module IV (13 hours) Quality and cost-characteristics of quality cost-micro analysis of quality cost-measurement of quality-TQM road map- ISO 9000 series certification-ISO 9001:2000 certification-ISO 14000 certification-QS 9000 auditing-Quality auditing- quality awards Text Books 1. L Suganthi, Anand A Samuel, Total Quality Management, PHI 2. Lt.Gen. Lal H, Total Quality Management, Wiley Eastern Limited Reference Books 1. Greg Bounds, Beyond Total Quality Management, McGraw Hill Publishers 2. Menon H G, TQM in New Product Manufacturing, McGraw Hill Publishers
University Examination Pattern PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. Analytical/Problem solving questions 4 x 5 marks=20 marks Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module.
PART B:
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PE09 L24: Industrial Psychology

Objectives To give awareness on the Human and Industrial Psychology
Module I (14 hours) Introduction- psychology as a science- area of applications study of individual- individual differences- study of behaviour- stimulus- response behaviour- heredity and environment- human mind- cognition- character- thinking- attention- memory- emotion- traits- attitude- personality Module II (14 hours) Organizational behaviour- definition development- fundamental concept- nature of peoplenature of organization an organizational behaviour system- models- autocratic model- hybrid model- understanding a social-system social culture- managing communication- downward, upward and other forms of communication Module III 13 hours) Motivation- motivation driver- human needs- behavior modification- goal setting- expectancy model- comparison models- interpreting motivational models- leadership- path goal model- style contingency approach Module IV (13 hours) Special topics in industrial psychology- managing group in organization- group and inter group dynamics- managing change and organizational development- nature planned changeresistance- characteristic of OD-OD process Text Books 1. Davis K. & Newstrom J.W., Human Behaviour at work, Mcgraw Hill International Reference Books 1. Schermerhorn J.R.Jr., Hunt J.G &Osborn R.N., Managing Organizational Behaviour, John Wiley 2. Luthans, Organizational Behaviour, McGraw Hill, International 3. Morgan C.t.,King R.A.,John Rweisz &John Schoples, Introduction to Psychology, McHraw Hill 4. Blum M.L. Naylor J.C., Harper & Row, Industrial Psychology, CBS Publisher Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
153
University Examination Pattern PART A: Short answer questions (one/two sentences) 5 x 2 marks=10 marks All questions are compulsory. There should be at least one question from each module and not more than two questions from any module. PART B: Analytical/Problem solving questions 4 x 5 marks=20 marks Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions 4 x 10 marks=40 marks Two questions from each module with choice to answer one question.
Maximum Total Marks: 70
PE09 L25: Entrepreneurship

Teaching scheme 3 hours lecture and 1 hour tutorial per week Objectives To give an idea on entrepreneurial perspectives Credits: 4
Module I (14 hours) Entrepreneurial perspectives- understanding of entrepreneurship process- entrepreneurial decision process- entrepreneurship and economic development- characteristics of entrepreneurentrepreneurial competencies- managerial functions for enterprise. Module II (14 hours) Process of business opportunity identification and evaluation- industrial policy- environmentmarket survey and market assessment- project report preparation-study of feasibility and viability of a project-assessment of risk in the industry Module III (13 hours) Process and strategies for starting venture- stages of small business growth- entrepreneurship in international environment- entrepreneurship- achievement motivation- time management creativity and innovation structure of the enterprise- planning, implementation and growth
154
Module IV (13 hours) Technology acquisition for small units- formalities to be completed for setting up a small scale unit- forms of organizations for small scale units-financing of project and working capital-venture capital and other equity assistance available- break even analysis and economic ratios technology transfer and business incubation Text Books 1. Harold Koontz & Heinz Weihrich, Essentials of Management, McGraw hill International 2 Hirich R.D. &Peters Irwin M.P., Entrepreneurship, McGraw Hill 3. Rao T.V., Deshpande M.V., Prayag Mehta &Manohar S. Nadakarni, Developing Entrepreneurship a Hand Book, Learning systems 4. Donald Kurado & Hodgelts R.M., Entrepreneurship A contemporary Approach, The Dryden Press 5. Dr. Patel V.G., Seven Business Crisis, Tata McGraw hill Timmons J.A., New venture Creation- Entrepreneurship for 21st century, McGraw Hill International 6. Patel J.B., Noid S.S., A manual on Business Oppurnity Identification, selections, EDII 7. Rao C.R., Finance for small scale Industries 8. Pandey G.W., A complete Guide to successful Entrepreneurship, Vikas Publishing Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class
155
CS09 L23 : Simulation and Modelling

Teaching scheme 3 hours lecture and 1 hour tutorial per week Objectives To teach the students how to reproduce real-world events or process under Credits: 4
controlled laboratory conditions, using mainly mathematical models.

Module I (10 hours)
Introduction - systems and models - computer simulation and its applications -continuous system simulation - modeling continuous systems - simulation of continuous systems discrete system simulation - methodology event scheduling and process interaction approaches - random number generation -testing of randomness - generation of stochastic variates - random samples from continuous distributions - uniform distribution exponential distribution m-Erlang distribution - gamma distribution - normal distribution - beta distribution - random samples from discrete distributions - Bernoulli - discrete uniform -binomial - geometric and poisson
ModuleII(12 hours)
Evaluation of simulation experiments - verification and validation of simulation experiments - statistical reliability in evaluating simulation experiments -confidence intervals for terminating simulation runs - simulation languages -programming considerations - general features of GPSS - SIM SCRIPT and SIMULA. ModuleIII(15 hours) Simulation of queueing systems - parameters of queue - formulation of queueing problems - generation of arrival pattern - generation of service patterns -Simulation of single server queues - simulation of multi-server queues -simulation of tandom queues.
Module IV (15 hours)
Simulation of stochastic network - simulation of PERT network - definition of network diagrams - forward pass computation - simulation of forward pass -backward pass computations - simulation of backward pass - determination of float and slack times determination of critical path - simulation of complete network - merits of simulation of stochastic networks. Reference Books 1. C. Deo N., System Simulation And Digital Computer, Prentice Hall of India. 2. Gordan G., System Simulation, Prentice Hall of India. 3. Law A.M. & Ketton W.D., Simulation Modelling and Analysis, McGraw Hill.
156
Internal Continuous Assessment (Maximum Marks-30) 60% - Tests (minimum 2) 30% - Assignments (minimum 2) such as home work, problem solving, group discussions, quiz, literature survey, seminar, term-project, software exercises, etc. 10% - Regularity in the class Note: One of the assignments shall be computer based simulation of continuous systems using any technical computing software One of the tests must be computer based (practical).
157
CS09 L24 : Computer Based Numerical Methods

Teaching scheme 3 hours lecture and 1 hour tutorial per week Objectives To impart the basic concepts of mathematical modelling of problems in science and engineering and to know procedures for solving different kinds of problems. To understand the various numerical techniques which provide solutions to non linear equations, partial differential equations etc that describe the mathematical models of problems. Module I (13 hours) Errors in numerical computation - mathematical preliminaries - errors and their analysis - machine computations - computer software. Algebraic and Transcendental Equations - bisection method - iteration method - method of false position - rate of convergence - method for complex root - Mullers method quotient difference method - Newton-Raphson method. ModuleII(13 hours) Interpolation introduction - errors in polynomial interpolation - finite differences - decision of errors Newtons formula for interpolation. Gauss, Sterling, Bessels, Everetts Formula - interpolation by unevenly spaced points - Lagrange interpolation formula - divided difference - Newtons general interpolation formula. ModuleIII(13 hours) Numerical Integration and Differentiation introduction - numerical differentiation - numerical integration - trapezoidal rule - Simpson 1/3 rule - Simpson 3/8 rule - Booles and Weddles rules - Euler-Maclariaun formula - Gaussian formula - numerical evaluation of singular integrals. Module IV (13 hours) Statistical Computations - frequency Chart - method of least square curve fitting procedures - fitting a straight line - curve fitting by sum of exponential - data fitting with cubic splines - approximation of functions. Regression Analysis - linear and nonlinear regression - multiple regression - statistical quality control methods. Text Books 1. E. Balagurusamy, Numerical Methods, Tata McGraw-Hill Pub.Co.Ltd, New Delhi, 1999. 2. C.F. Gerald and P.O. Wheatley, Applied Numerical Analysis, 6th Ed., Pearson Education Asia, New Delhi, 2002. Credits: 4
Reference Books
1. P. Kandasamy, K. Thilagavathy and K. Gunavathy, Numerical Methods, S.Chand Co. Ltd., New
Delhi, 2003.
2. R.L. Burden and T.D. Faires, Numerical Analysis, 7th Ed., Thomson Asia Pvt. Ltd., Singapore,
2002.
3. Shastri, Introductory methods of numerical analysis, Prentice Hall International. 4. V. Rajaraman, Introduction to Numerical Methods, Tata McGraw Hill.
158
CH09 L23 NANOMATERIAL AND NANOTECHNOLOGY

Teaching scheme 3 hours lecture & 1 hour tutorial per week Objectives To impart the basic concepts of nanotechnology To develop understanding about application of nanomaterials. No Pre-requisites Module 1 (13 Hours) Introduction to nanotechnology, nanoscale, electromagnetic spectrum, top down and bottom up approach, particle size, chemistry and physics of nanomaterials, electronic phenomenon in nanostructures, optical absorption in solids, quantum effects. Credits: 4
159
Module 2 (13 Hours) Nanomaterials, preparation of nanomaterials like gold, silver, different types of nano-oxides, Al2O3, TiO2, ZnO etc. Sol-gel methods, chemical vapour deposition, ball milling etc. Carbon nanotubes, preparation properties and applications like field emission displays. Different types of characterization techniques like SEM, AFM, TEM & STM. Module 3 (13 Hours) Nanocomposites, nanofillers, high performance materials, polymer nanocomposites, nanoclays, nanowires, nanotubes, nanoclusters etc. Smart materials, self assembly of materials, safety issues with nanoscale powders. Module 4 (13 Hours) Nanomanipulation, Micro and nanofabrication techniques, Photolithography, E-beam, FIB etc. Nanolithography., softlithography, photoresist materials. Introduction to MEMS, NEMS and nanoelectronics. Introduction to bionanotechnology and nanomedicines. References: 1. Nanocomposite science and technology, Pulikel M. Ajayan, Wiley-VCH 2005 2. Nanolithography and patterning techniques in microelectronics, David G. Bucknall, Wood head publishing 2005 3. Transport in Nanostructures, D.K. Ferry and S.M. Goodmick, Cambridge university press 1997. 4. Optical properties of solids, F. Wooten, Academic press 1972 5. Micro and Nanofabrication, Zheng Cui, Springer 2005 6. Nanostructured materials, Jackie Y. Ying, Academic press 2001 7. Nanotechnology and nanoelectronics, W.R, Fahrner, Springer 2005 8. Nanoengineering of structural, functional and smart materials, Mark J. Schulz, Taylor & Francis 2006. 9. Hand book of Nanoscience, Engineering, and Technology, William A. Goddard, CRC press 2003. 10. Nanoelectronics and Information Technology, Rainer Waser, Wiley-VCH 2003. 11. The MEMS Handbook Frank Kreith, CRC press 2002.
160
CH09 L25 PROJECT ENGINEERING

Teaching scheme 3 hours lecture & 1 hour tutorial per week Objectives To impart the basic concepts of project management No Pre-requisites Module 1 (13 hours) Scope of project engineering - the role of project engineer - R & D - TEFR - plant location and site selection - preliminary data for construction projects - process engineering - flow diagrams plot plans - engineering design and drafting Module 2 (13 hours) Planning and scheduling of projects - bar chart and network techniques - procurement operations - office procedures - contracts and contractors - project financing - statutory sanctions Module 3 (13 hours) Details of engineering design and equipment selection I - design calculations excluded - vessels heat exchangers - process pumps - compressors and vacuum pumps - motors and turbines - other process equipment Credits: 4
Module 4 (13 hours) Details of engineering design and equipment selection II - design calculations excluded - piping design - thermal insulation and buildings - safety in plant design - plant constructions, start up and commissioning
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References: 1. Rase & Barrow, Project Engineering of Process Plants, John Wiley 2. Peter S. Max & Timmerhaus, Plant design and economics for chemical engineers. 3. Mc Graw Hill (2002). 4. Srinath L. S., PERT AND CPM. affiliated east press pvt. Ltd., new york (1973) 5. Perry J. H.,Chemical engineering handbook 7TH ed. Mc Graw Hill ( 1997). 6. JELLEN F. C., Cost and optimization in engineering. Mc Graw Hill (1983). 7. Frederick B. Plummer, Project Engineering, BH 8. Ernest E. Ludwig, Applied project engineering and management, Gulf Pub. Co., (1988)
162
IT09 L24: MANAGEMENT INFORMATION SYSTEMS

Teaching scheme 3 hours lecture and 1 hour tutorial per week Objectives This course will introduce the methods and the influence of the information systems in management milieu and use MIS as an effective tool in management and decision making. Module - I: (12 hours) Information systems - functions of management - levels of management - framework for information systems - systems approach - systems concepts - systems and their environment effects of system approach in information systems design - using systems approach in problem solving - strategic uses of information technology Module - II: (10 hours) An overview of computer hardware and software components - file and database management systems - introduction to network components - topologies and types - remote access - the reasons for managers to implement networks - distributed systems - the internet and office communications Module - III: (14 hours) Application of information systems to functional - tactical and strategic areas of management, decision support systems and expert systems Module - IV: (16 hours) Information systems planning - critical success factor - business system planning - ends/means analysis - organizing the information systems plan - systems analysis and design - alternative application development approaches - organization of data processing - security and ethical issues of information systems Text Books 1. Robert Schultheis & Mary Sumner, Management Information Systems-The Manager's View, Tata McGraw Hill. Credits: 4
Reference Books 1. Laudon K.C. & Laudon J.P, Management Information Systems - Organization and
Technology, Prentice Hall of India 2. Sadagopan S, Management Information Systems, Prentice Hall of India 3. Basandra S.K, Management Information Systems, Wheeler Publishing. 4. Alter S, Information Systems: A Management Perspective, Addison Wesley. 5. Effy Oz, Management Information Systems, Thomson, Vikas Publishing House.
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Internal Continuous Assessment (Maximum Marks-30) University Examination Pattern 60% - Tests (minimum 2) PART Assignments (minimum 2) such as home work, problem solving, group2discussions, marks 5 x marks=10 quiz, 30% - A: Short answer questions (one/two sentences) literature survey, seminar, term-project, software exercises, etc. All questions are 10% - Regularity in the class compulsory. There should be at least one
question from each module and not more than two questions from any module.
PART B: Analytical/Problem solving questions
Candidates have to answer four questions out of six. There should be at least one question from each module and not more than two questions from any module. PART C: Descriptive/Analytical/Problem solving questions Two questions from each modu le with choice to answer 4 x 10 marks=40 marks

BT Syllabus - 2009

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UNIVERSITY OF CALICUT

B.TECH BIOTECHNOLOGY CURRICULUM And SYLLABUS

(1&2 SEMESTER 8TH SEMESTER)

EN09 101 EN09 102 EN09 103

Engineering Mathematics I Engineering Mathematics II Engineering Physics

EN09 103(P) Physics Lab EN09 104 Engineering Chemistry

B. TECH. BIOTECHNOLOGY THIRD SEMESTER

B. TECH. BIOTECHNOLOGY FOURTH SEMESTER

B. TECH. BIOTECHNOLOGY FIFTH SEMESTER

BT 09 501 BT 09 502 BT 09 503 BT 09 504 BT 09 505 BT 09 506

B. TECH. BIOTECHNOLOGY SIXTH SEMESTER

BT 09 601 BT 09 602 BT 09 603 BT 09 604 BT 09 605 BT 09 LXX

BT 09 701 BT 09 702 BT 09 703 BT 09 704 BT 09LXX BT 09 LXX

BT 09 801 BT 09 802 BT 09 LXX BT 09 LXX

BT 09 805(P) Seminar BT 09 806(P) Project BT 09 807 Viva-voce Total

GLOBAL ELECTIVES FROM OTHER BRANCHES

EN09 101: Engineering Mathematics I

EN09 102: Engineering Mathematics II

EN09 103: Engineering Physics

EN09 103(P) : Physics Lab

(Minimum 12 experiments should be completed)

EN09 104: Engineering Chemistry

EN09 104(P): Chemistry Lab

4. 5. 6. 7. 8. 9. 10. 11. 12.

EN09 105: Engineering Mechanics

EN 09 106: Basics of Civil and Mechanical Engg.

Section1: Basicsof CivilEngineering

22 Section2: BasicsofMechanical Engineering

EN09 107: Basics of Electrical, Electronics & Communication Engineering

EN09 108: Engineering Graphics

Teaching scheme 3 hours drawing per week

EN09 109(P): Computer Programming in C

20% - Viva voce 10% - Record

EN09 110(P): Mechanical Workshops

EN09 111(P) Electrical and Civil Work shops

EN09 301: ENGINEERING MATHEMATICS III

EN09 302: HUMANITIES AND COMMUNICATION SKILLS

BT09 303 FLUID FLOW OPERATIONS

BT 09 306 BIOPROCESS CALCULATIONS.

BT 09 307 (P) MICROBIOLOGY LABORATORY

BT 09 308 (P) BIOCHEMISTRY LABORATORY

EN09 402: Environmental Science (Common for all branches)

University Examination Pattern

BT 09 403 CHEMICAL REACTION ENGINEERING

University Examination Pattern

3 hrs lecture and 1 hr tutorial per week Objectives:

University Examination Pattern

University Examination Pattern

B T 09 406 MECHANICAL OPERATIONS

University Examination Pattern

FLUID MECHANICS & MECHANICAL OPERATIONS LAB

60% 30% 10% 50

BIOANALYTICAL TECHNIQUES LAB

To provide experience in various bioanalytical techniques in biotechnolog y

60% 30% 10% 50

BT09 501 MASS TRANSFER OPERATIONS

University Examination Pattern

BT 09 502 MOLECULAR BIOLOGY

University Examination Pattern

BT09 503 ENZYME SCIENCE & ENGG.

THERMODYNAMICS AND HEAT TRANSFER

Teaching Scheme: 3 Hrs lecture and 1 hr tutorial per week Objective:

University Examination Pattern

3 hours lecture and 1 hour tutorial per week Objective: