I. Title: Experiment 1: Microscopy II.

Objectives: to be familiarized with the parts of the microscope to be able to know how to use the microscope III. Materials Compound microsope Specimen Cedar oil IV. Procedure Turn the revolving nosepiece to LPO. Place the microscope slide on the stage and fasten it with the stage clips. Using the coarse adjustment, lower the objective lens down as far as it will go. Look through the eyepiece and adjust the illuminator and diaphragm for the great est amount of light. Slowly turn the coarse adjustment. Followed by fine adjustment to sharpen the im age. Move the microscope slide around so that the image is in the center of the field of view. Draw the image seen under the microscope. Turn the revolving nosepiece to HPO. Sharpen image by adjusting fine adjustment. Draw the image seen. Put a drop off cedar oil at the specimen. Turn the revolving nosepiece to OIO. Sharpen image by adjusting fine adjustment. Draw the image seen. V. Results and observations Specimen: Clostridium botulinum

LPO HPO OIO VI. Post lab questions 1. Differentiate resolving power and magnifying power. 2. Why is oil necessary when using the OIO? 3. In microbiology, which is the most commonly used objective? Why? 4. What happens to the side of lens and the light as you increase the magni fication? VII. Answers for the post lab questions. 1. The smallest angular separation that can be unambiguously distinguished is called the resolving power. While magnifying power is relevant only when an eyepiece is used to magnify the image for visual inspection. The angular size of the virtual image seen by the observer will be larger than the actual angular s ize of the object. 2. Because light is refracted every time it passes through a medium with a different refractive index, (air to glass or vice versa).Immersion oil has been formulated so that it has a refractive index identical to that of glass. Thus th ere is no refraction of light when it passes from glass to oil and vice versa. 3. OIO because it can view the specimen (microbes) 1000X, much clearer than LPO and HPO. 4. The side of the lens becomes thicker as you increase the magnification a nd the light that passes through is being refracted. References: http://suite101.com/article/viewing-bacteria-under-oil-immersion-a62401 telescope: Resolving and Magnifying Power Infoplease.com http://www.infoplease. com/ce6/sci/A0861462.html#ixzz1xNsVsLFF