Photometric colorimetric test for Magnesium with Lipid Clearing Factor (LCF) PACKAGE SIZE E10010 2 x 100 ml Complete

Test Kit

METHOD Magnesium ions in an alkaline medium form a colored complex with xylidyl blue. The absorbance increase is proportional to the magnesium concentration in the sample. Glycoletherdiamine-N, N, N', N'-tetraacetic acid (GEDTA) is used as masking agent for calcium ions. CONTENTS 2 x 100 ml Color reagent CAPS GEDTA Xylidyl blue Sodium azide Activators 1 x 3 ml Standard Magnesium (II) Sodium azide

CV % 2.16 2.19 Linearity The test is linear up to a magnesium concentration of 5 mg/dl or 0.25 mmol/l. Dilute samples with a higher concentration 1 + 1 with physiological saline (0.9%). Multiply the result by 2. 5. Correlation coefficient (r) = 0.941 6. Sensitivity: 0.043A on mg/dl 7. Detection limit: 0.02 mg/dl 8. Interferences: The test is not influenced by lipemic sera or bilirubin concentrations up to 20 mg/dl. Do not use hemolytic sera due to the high magnesium concentration in erythrocytes. These characteristics have been obtained using a manual procedure. DIAGNOSTIC CHARACTERISTIC The magnesium blood plasma homeostasis is the resultant of the process of intestinal magnesium absorption, bone turnover and renal clearance. Aldosterone increases and the parathormone reduce magnesium excretion with urine. NOTES 1. For professional use only. 2. For in vitro use only. 3. Adaptation sheets for automatic analyzers can be made available on request. 4. Chemicals residues and remains should be according to local law regulation. 5. Additional equipment: photometer or automated chemistry analyzer able to read at Hg 546, 520 nm. 6. [RGT] and {STD] contains sodium azide. Do not swallow. Avoid contact with skin and mucous membranes. 7. Contaminated glassware is the greatest source of error. Disposable plastic ware is recommended for the test 8. Lipemic specimens usually generate turbidity of the sample/reagent mixture which leads to false high results MAGNESIUM test avoids these false high results through its built-in Lipid Clearing Factor (LCF). The LCF clears a turbidity caused by lipemic specimens up to 2000 mg/dl triglycerides. REFERENCES 1. Mann, C.K., and Yoe, J.H., Anal.Chem. 28, 202-205 (1956) 2. Mann, C.K., and Yoe, J.H., Anal.Chim.Acta 16, 155-160 (1957) 3. Bohuon, C., Clin. Chim. Acta 7, 811-817 (1962) 4. G. Weiss, Diagnostische Bewertung von Laborbefunden; J.F. Lehmanns Verlag Mnchen (1976)

49 mmol/l 0.13 mmol/l 0.09 mmol/l 0.095 % 2.5 mg/dl or 1.03 mol/l 0.095 %

REAGENT PREPARATION AND STABILITY [RGT] and [STD] are ready for use. [RGT] and [STD] are stable even after opening up to the stated expiry date when stored at 2-25C. Avoid contamination. SPECIMEN Serum, plasma (do not use EDTA-plasma!), liquor and urine. Bring the urine to pH 3 - 4 by adding some drops of conc. hydrochloric acid. Dilute further 1 + 4 with distilled water. Multiply the result by 5. ASSAY Wavelength: Optical path: Temperature: Measurement: 520 nm, Hg 546 nm 1 cm 20-25C against reagent blank

PIPETTING SCHEME Pipette into cuvettes Reagent blank Sample / [STD] Sample / [STD] --10 l Distilled water 10 l --[RGT] 1000 l 1000 l Mix, incubate for 10 minutes at 20-25C. Measure the absorbance of the sample and [STD] against the reagent blank within 60 minutes (A). CALCULATION OF THE MAGNESIUM CONCENTRATION C = 2.50 x [mg/dl] C = 1.03 x [mmol/l] NORMAL VALUES Serum, plasma 1.9 - 2.5 mg/dl 0.8 - 1.0 mmol/l Liquor 2.5 - 3.5 mg/dl 1.0 - 1.5 mmol/l Urine 1 - 10 mg/dl 0.4 - 4.1 mmol/l 24 h urine 50 - 150 mg / 24 h 2.0 - 6.2 mmol / 24 h These values are for orientation purpose; each laboratory should establish its own reference range. QUALITY CONTROL All control sera with values determined by this method can be used. We recommend using our HUMATROL quality control sera, based on animal sera or our SERODOS based on human sera. PERFORMANCE CHARACTERISTICS Reagent blank wavelength 546 nm absorbance 1.400 2. Precision intra assay Level I II N 15 15 Mean concentration mg/dl 2.29 4.01 CV% 1.69 1.85 Precision inter assay Level I II N 15 15 Mean concentration mg/dl 2.34 4.15 or

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Z.Jakubowski,J.Kabata.L.Kalinowski,M.Szczepaska-Konkel, S.Angielski Laboratory tests in every day practice. 1996 MAKmed Gdask ISO 15223 Medical devices Symbols to be used with medical device labels, labelling and information to be supplied. Explanation of symbols instructions Consult
for use Batch code Use by

Caution, consult accompanying documents For in vitro diagnostic use Temperature limitation

Manufacturer Catalog number

Update: 01.08.2006 [JPW] Print: 10.08.2006 [JPW]

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Sp. z o. o.

Budowlanych 68 80-298 Gdansk POLAND

tel. (48 58) 556 52 46 (48 58) 556 52 31 fax (48 58) 341 12 49

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