Professional Documents
Culture Documents
THE BACTERIA
AND ARCHAEA
Prepared by
Brenda Leady, University of Toledo
1 reprod
Copyright (c) The McGraw-Hill Companies, Inc. Permission required for
One of the most prominent features of the
bacteria and archaea is their diversity
Only 1% of newly discovered species
have been cultured in the lab
Most species are known only as distinctive
molecular sequences
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Prokaryotes
Lack nuclei and other cellular features typical
of eukaryotes
Domain Archaea
Domain Bacteria (or Eubacteria)
4
Domain Archaea
Possess a number of features in common
with the eukaryotic nucleus and
cytoplasm, suggesting common ancestry
Histones
Membrane linkages different from those in
eukaryotes or bacteria
More resistant to heat and other extreme
conditions
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Extremophiles
Can occupy habitats with very high salt
content, acidity or methane levels, or high
temperatures
Methanopyrus grows in deep-sea thermal
vents at 98°C
Sulfolobus grows in hot springs at pH3
Halophiles
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Domain Archaea
Kingdom Crenarchaeota
Sulfolobus and others that grow in extreme
hot or cold
Kingdom Euryachaeota
Methane producers and extreme halophiles
Kingdom Korarchaeota
Hot springs
Kingdom Nanoarchaeota
Hyperthermophiles
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Domain Bacteria
50 or so bacterial phyla
Structural and metabolic features of half
unknown
Many more bacteria favor moderate
conditions
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Proteobacteria
Amazing diversity of form and metabolism
5 major subgroups
α-proteobacteria
Ancestors of mitochondria, Rhizobium,
Agrobacterium
β-proteobacteria
Nitrosomonas, Neisseria
γ-proteobacteria
Vibrio, Salmonella, Escherichia coli
δ -proteobacteria
Myxobacteria, bdellovibrios
ε –proteobacteria
Helicobacter 11
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Cyanobacteria
Photosynthetic bacteria abundant in fresh
waters, oceans and wetlands and on
surfaces of arid soils
Named for blue-green or cyan color
The only prokaryotes that generate
oxygen as a product of photosynthesis
Gave rise to plastids of eukaryotic algae
and plants
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Display the greatest structural diversity
found among bacterial phyla
Singlecells or colonies
Filaments
Essential ecological roles in producing
organic carbon and fixing nitrogen
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Horizontal gene transfer
Also known as lateral gene transfer
Movement of one or more genes from one
species to another
Contrasts with vertical gene transfer from
parent to progeny
Horizontal gene transfer increases genetic
diversity
Influences the methods used to infer the
phylogeny of bacteria and archaea
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Can result in large genetic changes
At least 17% of the genes present in the
common human gut inhabitant E. coli
came from other bacteria
Allowing new metabolic processes to be
acquired despite lacking the sexual
processes typical of eukaryotes.
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Potential to interfere with human efforts to
deduce evolutionary relationships
Molecular systematists employ ribosomal
RNA (rRNA) genes and other sequences
thought to less often move horizontally
and thus more accurately reflect patterns
of vertical inheritance
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Important concepts
Bacteria and Archaea evolved from a common
ancestor
Eukaryotic nucleus and cytoplasm likely arose in
an ancient archaeal organism
Mitochondria and plastids originated from
proteobacteria and cyanobacteria by
endosymbiosis
Bacteria and archaea are amazingly diverse, but
many phyla and species lack scientific names
because microbiologists know so little about
them 19
Structure and motility
Bacteria and archaea share small size,
rapid growth, and simple cellular structure
Bacteria and archaea are 1–5 μm in
diameter
(most plant and animal cells are between 10
and 100 μm in diameter)
Small cell size limits the amount of
materials that can be stored within cells
but allows faster cell division
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Cellular structure
Prokaryotic cells are
much simpler than
eukaryotic cells
Thylakoids –
ingrowths of plasma
membrane that
increase surface area
for photosynthesis
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Magnetosomes –
magnetite crystals
Compass like
Helps to locate low-
oxygen habitats
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Other examples of cell structure
complexity
Nucleus-like bodies from plasma membrane
invaginations
Cellular proteins similar to eukaryotic tubulin
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Cell shape and arrangement
5 major shapes
Spheres – cocci
Rods – bacilli
Comma-shaped – vibrios
Spiral-shaped – spirochaetes are flexible
while spirilli are rigid
Some occur as single cells, pairs,
filaments
Important diagnostic features
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Mucilage
Composed of polysaccharides,
protein, or both
Secreted from cells
Functions
Evade host defenses
Hold colony together – biofilms
Dental plaque
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Cell-wall structure
Maintain cell shape and help protect
against attack
Also help avoid lysis in hypotonic solutions
Archaea and some bacteria use protein
Most bacteria use peptidoglycan
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Gram stain
Gram positive
Relatively thick peptidoglycan layer
Purple dye held in thick layer
Cells are stained purple
Vulnerable to penicillin that interferes in cell wall
synthesis
Gram negative
Less peptidoglycan and a thin outer envelope of
lipopolysaccharides
Lose purple stain but retain final pink stain
Cell are stained pink
Resists penicillin and requires other antibiotics 29
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Motility
Move to favorable conditions
Respond to chemical signals
Swim, twitch, glide or adjust floatation
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Flagella
Swimming
Different from
eukaryotic flagella
Like an outboard boat
motor
Differ in number and
location of flagella
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Pili
Twitch or glide
Threadlike cell
surface structures
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Gas vesicles
Cyanobacteria
Adjust buoyancy
Move up or down in water column
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Binary Fission
Divide by splitting in two
Basis for widely used method of detecting
and counting bacteria in samples
Place measured volume of sample into plastic
dishes of agar
Single cells will form visible colonies
Can also use fluorescent dye that binds
bacterial DNA to directly count bacteria
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Surviving harsh conditions
Akinetes
Develop when stressed
Can germinate into metabolically active cells
under favorable conditions
Aquatic filamentous cyanobacteria
Endospores
Tough protein coat
Amazingly long dormant span
Bacillus anthracis, Clostridium botulinum,
Clostridium tetani
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Obtaining genetic material
Transduction
Via viral vector
Transformation
Via uptake of DNA from environment
Conjugation
Via mating with another cell
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Nutrition and metabolism
More diverse types of metabolism than
any other group of organisms
Can be classified by
Nutrition
Response to oxygen
Presence of specialized metabolic processes
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Nutrition classification
Autotrophs
Produce all or most of their own organic compounds
Photoautotroph – uses light as energy source for
synthesis of organic compounds from CO2 or
H2S
Chemoautotrophs – use energy obtained from
chemical modification of inorganic compounds to
synthesize organic compounds
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Heterotrophs - organisms that require at
least one organic compound, and often
more
Photoheterotroph – able to use light
energy to make ATP but they must take in
organic compounds from the environment
Chemoorganotroph – must obtain organic
molecules for both energy and carbon
source 44
Classification by oxygen response
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Classification by special metabolism
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Gene Expression Studies Revealed How
Cyanobacteria Fix Nitrogen in Hot Springs
Thermal pools display multicolored microbial
mats
Composed of diverse nutrition types
In Yellowstone, Synechococcus are the only
photoautotrophs
High temperatures allowed few nitrogen
fixers
Synechococcus was producing its own fixed
nitrogen
Tracked process using gene expression
Ecological roles
Carbon cycle
Producers synthesize organic compounds
used by other organisms as food
Decomposers (saprobes) break down dead
organisms to release minerals for reuse
Methanogens make methane
Methanotrophs consume methane
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Symbiotic roles
Mutualism
Association beneficial to both partners
Many aquatic protists depend on bacterial
partners for vitamins
Parasitism
One partner benefits at the expense of the
other
Pathogens – cholera, leprosy, tetanus,
pneumonia, Lyme Disease, etc.
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Industrial and other roles
Dairy products (cheese and yogurt)
Vinegar, amino acids, enzymes, vitamins,
insulin, vaccines, antibiotics, etc.
Useful in treating wastewater, industrial effluent,
and other harmful substances
Bioremediation
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The Daly Experiments Revealed How Deinococcus
radiodurans Avoids Radiation Damage