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(BS20001)
What is Respiration?
The process of converting Food Energy into Chemical Energy (ATP).
Photosynthesis vs Respiration
Photosynthesis
Produces food Stores energy Uses water Uses CO2 Releases O2 Occurs in light Occurs only in cells containing chloroplasts
Respiration
Uses food Releases energy Produces water
Produces CO2 Uses O2 Occurs at all time Occurs in all cells
Glucose
Oxygen
Carbon dioxide
Water
Energy
Glucose
Oxygen
Carbon dioxide
Water
What is ATP?
Energy currency of the cell Adenosine Triphosphate 5-Carbon sugar (Ribose) Nitrogenous base (Adenine) 3 Phosphate groups The chemical bonds that link the phosphate groups together are Covalent high energy bonds When a phosphate group is broken down to form ADP and Pi, small packets of energy are released.
Intermembrane space
Cellular Respiration
34
GLYCOLYSIS
Glyco = sweet Lysis= splitting
Embden-Meyerhoff-Parnas (EMP) Pathway Anaerobic (does not require Oxygen) 10 steps all occurring in cytosol or cytoplasm
GLYCOLYSIS
Glycolysis Summary
Takes place in the Cytosol (cytoplasm) Doesnt Use Oxygen
Pyruvate dehydrogenase
Krebs cycle
Each turn of the Krebs Cycle also produces 3NADH, 1FADH2, 1ATP and 2CO2 Therefore, For each Glucose molecule, the Krebs Cycle produces 6NADH, 2FADH2, 2ATP and 4CO2
Consists of 5 complexes: Complex I (NADH dehydrogenase) Complex II (Succinate dehydrogenase) Complex III (Ubiquinone-Cytochrome bc1 complex) Complex IV (Cytochrome oxidase) Complex V (ATP synthase)
Chemiosmosis
The steps that transport protons from Intermembrane space to matrix establishing a proton chemiosmotic gradient. It is an energy-coupling mechanism that uses energy stored in the form of an H+ gradient across a membrane to generate ATP.
F1
The F0 complex making up the proton pore is composed of three subunits, a, b, and c, in the proportion ab2c10-12. The F1 has nine subunits of five different types (33 ) Matrix The subunits undergoes three three coformational chages ( -empty, -ADP and ATP). The proton gradient causes the enzyme to release the newly synthesized ATP from its surface.
F0
Intermembrane space
ATP synthase
F1
F0
ATP Synthesis
Inner mitochondrial membrane is impermeable to protons. Proton can reenter the matrix only through proton-specific channels (F0). The proton-motive force that drives protons back into the matrix provides the energy for ATP synthesis, catalysed by the F1 complex associated with F0.
NADH
Pyruvate decarboxylase
Lactate dehydrogenase
Lactic acid fermentation occurs in animal muscle cells, some fungi and bacteria to make yogurt
Fermentation
Occurs when O2 NOT present (anaerobic) Requires NADH generated by glycolysis Called Lactic Acid fermentation in muscle cells, some fungi and bacteria, produces lactic acid)
Fate of Glucose
Glucose
glycolysis (10 successive reactions) anaerobic conditions anaerobic conditions
2 Pyruvate
O2 aerobic conditions 2CO2
2 Lactate
Fermentation to lactate in vigorously contracting muscle, in erythrocytes, and in some microorganisms
4CO2 + 4H2O
Animal, plant, and many microbial cells under aerobic conditions
efficiency
2.2%efficiency
Food
Polysaccharides
Fats
Proteins
Sugars
Glycerol
Fatty acids
Glycolysis
AcetylCoA
Krebs Cycle
Electron Transport