Professional Documents
Culture Documents
ARVIND KUMAR
Current position: Senior Scientist 1, Plant Breeder Drought, Aerobic Rice
M.Sc. 1992:
B.Sc. 1989:
Work experience: Jan 19, 2010 present : Jan 19, 2007 Jan 18, 2010: July 1, 2006 - Jan 18, 2007 : Oct 30,2004 - Jun 30, 2006 : Jan 25, 1995 - Oct 29, 2004 : Nov 4, 1992 - Jan 24, 1995 :
Senior Scientist I, PBGB, IRRI Senior Scientist, PBGB, IRRI Senior Scientist, IGKV, India Post Doc Fellow, IRRI Scientist/Senior Scientist, IGKV Research Associate, IGKV
Research highlights: Varieties developed/ germplasm registered: 21 Genes/QTLs identified : 15 (7 genes for gall midge resist., 8 QTLs for DT) Book Chapters : 19 Manuscripts in peer reviewed journals : 76 Thesis Advisor : Post Doctoral: 5; Ph.D.: 7; M.S.: 5
Awards: 1. Young Scientist Award 96-97 from Madhya Pradesh Council of Science & Technology, India 2. Best Scientist Award of IGKV, Raipur for year 2002
GSR
Afrida Abu Hassan
Increased water uptake- not always due to root depth, lateral roots and/or root hairs play important role also
Major drought yield QTLs are true and effective Effect of individual QTLs 300-500 kg ha-1, requires pyramiding of three QTLs to get 1.2 ton or more yield advantage Major QTLs with effect in the multiple improved genetic background exists, need to put in more efforts to identify something like sub 1 Vandana, Way Rarem, IR64 improved for yield under drought. DTY QTLs introgression in Swarna, Swarna sub 1 under progress As we move to deliver molecular breeding products in project driven research, efficient testing facility at IRRI required
Sahod Ulan 1 Hardinath 1 Sahbhagi dhan BRRI dhan 56 Sookha dhan 3 Sookha dhan 1 Sookha dhan 2
IR79913-B-176-B-4 IR81412-B-B-82-1
IR81023-B-116-1-2 IR72667-16-1-B-B-3 IR74963-262-5-1-3-3 IR79971-B-191-B-B IR79971-B-227-B-B
105 120
115 115 125 110 113
90 107
130 100 100 115 114
2.000
1.500
NON STRESS index values of top 20 entries tested from 2008-2010
1.000
0.500
0.500 0.000
1 2 3 4 5 6 7 8 9 1011121314151617181920
0.000
1 2 3 4 5 6 7 8 9 1011121314151617181920
3.500 3.000
2.500
2.000 1.500 1.000 0.500 0.000
SEVERE STRESS index values of top 20 entries tested from 2008-2010
1 2 3 4 5 6 7 8 9 1011121314151617181920
IR64
qDTY1.1
qDTY2.2 qDTY4.1 qDTY9.1
N22
Adaysel Adaysel Adaysel
Lowland
Lowland Lowland Lowland
24
14 6 29
17
6 11 19
qDTY10.1
Swarna qDTY1.1 qDTY1.1 qDTY2.1 qDTY3.1 Sabitri qDTY12.1 qDTY3.2 Samba Mahsuri TDK1 qDTY11.1 qDTY3.1 qDTY6.1 qDTY6.2 MTU1010 BR11 qDTY1.1 qDTY3.1
Adaysel
N22 Dhagaddeshi Apo Apo IR74371-46-1-1 IR77298-5-6-18 IR55419-04 IR55419-04 IR55419-04 IR55419-04 N22 Apo
Lowland
Lowland Lowland Lowland Lowland Lowland Lowland Lowland Lowland Lowland Lowland Lowland Lowland
18
29 25 23 30 25 16 32 10 12 16 16 27
17
13 32 7 27 47 19 14 7 9 10 13 25
Large effect drought QTLs qDTY1.1, qDTY2.3, qDTY3.1, qDTY3.2, qDTY12.1 shows effect against Different drought susceptible recipient varieties Different environments Across both lowland and upland ecosystem
No guarantee if MAB is not carried by an experienced drought breeder as many minor modifications are needed during the process of MAB
Apo/IR64 Apo/IR72
IR64*2/Azucena Vandana/IR64 Vandana/IR72
52.2 63.6
36.2 25.6 63.4
Upland Upland
Upland Upland Upland
IRRI IRRI
IRRI IRRI IRRI
N22/IR64
N22/MTU1010 Dhagaddeshi/Swarna Dhagaddeshi/IR64
24.3
16.1 24.9 8.3
Lowland
Lowland Lowland Lowland
IRRI
IRRI IRRI IRRI
Prashant, IRRI
Chr
3 3
Interval
RM520-RM16030 RM15935-RM520
R2
30 20
Additive effect 25 22
Donor
Apo Apo
Recipient
Swarna BR11
Apo x Swarna
Apo x BR11
Swamy, IRRI
Introgression of major effect drought grain yield QTLs DTY3.1 and DTY12.1 Anjali
IR81896-B-195 ( DTY3.1) F1 X Anjali (WS2010) Fore ground selection QTLs DTY3.1 BC1 X Sub1Swarna (DS2011) Fore ground selection DTY 12.1 Chr 3 12 X Anjali (DS2010) Fore ground selection
BC2F1 (WS2011)
BC2F2 (DS2012)
IR 84984-83-15-18-B-B-93 X Anjali (DS2010) Fore ground selection ( DTY12.1) F1 X Anjali (WS2010) Fore ground selection
BC2F1 (DTY3.1)
BC3F2 (WS2012)
Selected homozygotes with DTY3.1, DTY12.1 and their combinations will be tested under drought DS2013
Swamy, IRRI
Upland
Additive effect of QTL as % of trial mean
Lowland
IR74371-46-1-1/ Sabitri
Mishra et al. 2012, BMC Genetics (Revision)
qDTY2.3, qDTY3.2 were also detected to show effect in Vandana/Way Rarem in upland
BC2F2 population screened for blast tolerance 361BC2F3 derived lines tolerant to blast for mapping yield QTLs under stress and non- stress
QTLs for grain yield under drought stress and non- stress conditions
At present 381 single plant selections from the population being screened in India
A subset of 100 lines being screened at IRRI under aerobic conditions
S. Dixit, IRRI
Chr
Interval
Severe Stress Add LOD R2 id2002229 18.8 60.4 4.7 5.8 k_9 112.8 33.9 8.8 10.6 id3000757 5.1 43.4 10.8 12.9 ud12000118 11.0 55.1 4.0 5.0 id12005428 61.7 id12007988 89.0 31.0 5.2 6.4 Peak Position
Moderate stress Combined Add LOD R2 Add LOD R2 10.6 4.1 10.6 5.6 7.2 3.8 5.1 19.0 9.7 7.0 18.8 12.5 25.4 3.9 4.8 12.1 4.1 11.7 14.9 4.9 5.1
S. Dixit, IRRI
90 IR64 77
3
4 5 6 7 8
IR 87707-182-B-B-B
IR 87707-118-B-B-B IR 87729-69-B-B-B IR 87706-215-B-B-B IR 87705-44-4-B-B IR 87705-14-11-B-B
78
83 80 80 78 78
89
93 95 90 95 90
69
82 80 61 81 61
59
74 56 51 50 61
2863
1209 2073 1506 1861 2180
451
102 97 174 113 439
9
10 11
IR 87705-83-12-B-B
IR 87728-102-B-B-B IR64
83
86 83
95
95 90
69
76 77
54
54 65
1920
1542
114
54
IR 1383 64 96
RIL
RIL
4
8
6 1, 1 2 2
RIL BIL
1 4 29
No second example of such a massive effort to identify QTLs The efforts resolved many misgivings about drought yield QTLs
Dhagaddeshi
Dhagaddeshi
Swarna
Swarna
BH
BL
RM11943
RM431
ADVANTAGES of BSA
Cost effective
Can be applied with expensive marker systems like SNP (Becker et al. 2011-Plos One) RNA can be pooled for microarray (Kadam et al. 2012)
Phenotypic selection
Background genotyping Being Screened at IRRI under ROS Plants with clear background Phenotypically and genotypically Background selection
Drought screening
Vikram, Prashant
Introgression and pyramiding of identified QTLs for Maintenance breeding improve popular high yielding varieties- Vandana, IR64, Swarna, Sabitri, TDK 1 keeping the other traits intact Forward breeding- From segregating BC2F2 population select lines with high yield irrespective of background clearance, and traits different from the recipient variety Develop near isogenic lines in improved background with different QTL combinations for basic as well as applied research
Marker assisted Breeding Strategy for high grain yield under drought
Mega varieties Swarna, IR64, MTU1010, TDK, Vandana Sabitri, Sambha Mahsuri
F1 F2
x
F1 BC1F1 BC2F1 BC2F2 BC2F3
QTLs identified for high grain under drought using BSA/ WG Consistent and major effect QTLs selected for MAS in each recipient background
Lines with QTLs and combinations identified from the mapping populations and Intercrossed/back crossed to recipient parents BC1F1(500)/ BC2F1 ( 1000) / BC3F1(1500-2000) lines with all the QTLs Selected
Selection of BC3F2 (4000-5000) homozygous lines and background genotyping (To break DTF and HT linkages)
Screening under managed drought stress and non-stress (IRRI) Phenotyping Selected best lines evaluated for grain quality and Other parameters Best lines evaluated in target environments of the respective variety
Multi-location evaluation
- QTL
IR64
IR64+DTY QTLs
IR 64
Product - 2011
+ QTL line
IR64 QTL lines under testing in WS2011, WS2012 at 14 locations in India under AICRIP program; Nepal (03), Bangladesh (01), Philippines (01),
IR 87729-69-B-B-B IR 87728-491-B-B
78
80 77 84 80 81 80 3
99
98 96 94 95 89 96 7
4550
3752 5045 4796 3850 2987
6103
4388 5844 6115 5526 5516 5435 1053
2068
2556 2555 1147 1916 2074 636
2632
3000 3023 1636 2270 2151 1442 690
96.9
97.0 96.9 92.4 95.0 94.6
Improved drought tolerant IR64 lines under lowland drought in target environments
Grain yield (kg ha-1) Rajshahi Entry IR87707445-B-B IR87707446-B-B IR87707182-B-B IR64 LSD NS 4167 4521 4312 3379 240 DS 1525 1933 1508 980 156 Nepalganj NS 5990 6302 6510 4297 324 DS 3472 2847 2778 1597 170 Raipur NS 5084 5771 5646 4083 434 DS 3956 3614 3419 2662 285 Hyderabad 1 NS 5672 5634 6047 5699 186 DS 1684 1813 1383 660 939 Hyderabad 2 NS 5672 5634 6047 5699 186 DS 3800 4057 3604 3085 600 Hazaribagh NS 5690 5711 4453 5612 475 DS 1604 1229 1500 958 422 NS 4100 4911 4513 3586 920 Rewa DS 3731 3899 3509 2503 909
Swamy, 2012
90 IR64 77
3
4 5 6 7 8
IR 87707-182-B-B-B
IR 87707-118-B-B-B IR 87729-69-B-B-B IR 87706-215-B-B-B IR 87705-44-4-B-B IR 87705-14-11-B-B
78
83 80 80 78 78
89
93 95 90 95 90
69
82 80 61 81 61
59
74 56 51 50 61
2863
1209 2073 1506 1861 2180
451
102 97 174 113 439
9
10 11
IR 87705-83-12-B-B
IR 87728-102-B-B-B IR64
83
86 83
95
95 90
69
76 77
54
54 65
1920
1542
114
54
IR 1383 64 96
IR 87707-445-B-B-B
IR 87707-182-B-B-B
IR64
Similar RVA and cooking quality traits for IR64 & IR64 NILs
ENTRY IR 87706-215-B-B-B IR 87705-14-11-B IR 87705-83-12-B IR 87707-445-B-B-B IR 87728-102-B-B IR 87707-446-B-B-B IR 87705-444-B IR 87729-69-B-B-B IR 87707-182-B-B-B IR64
3500 3000 2500 2000 1500 1000 500 0 0.0 -500 2.0 4.0 6.0 8.0 10.0 12.0 14.0
Peak 1 Trough 1 Breakdown Final Visc Setback Peak Time Pasting Temp Retro
2932 2845 2793 2689 2627 2828 2778 2681 2646 2799
1356 1405 1348 1521 1311 1561 1433 1288 1404 1462
1576 1440 1445 1168 1316 1267 1345 1393 1242 1337
2840 2750 2633 2868 2620 2938 2798 2631 2793 2825
5.6 5.67 5.67 5.87 5.67 5.87 5.73 5.67 5.73 5.8
1484 1345 1285 1347 1309 1377 1365 1343 1389 1363
IR87706-215-b-b-b
Aday sel
LINE
Stress GY (Moderate) 3141 2566 3389 3438 3025 3017 2326 717
Non stress GY (Combined) 2613 1979 2124 2398 1832 2336 1211 384 GYC 5118 6496 4418 5474 4569 4499 4282 1701 DTFC 86 88 91 91 86 81 99 95
Grain type Similarity HTC % Swarna Class to Swarna 98 90 Yes Medium 96 90 No Medium 93 95 93 83 91 120 91 92 93 95 No Yes Yes Yes Medium Medium Medium Medium
S. Dixit, IRRI
S. Dixit, IRRI
QTL qDTY2.1 qDTY3.3 qDTY4.2 qDTY4.3 qDTY6.3 qDTY6.2 qDTY8.2 qDTY8.3 qDTY9.2 qDTY9.3 qDTY11.2 qDTY12.3
Chr
Markers
Peak
Position
2 id2004861- id2007213 id2006621 54.8 3 id3010094-ud3001370 id3010740 95.1 4 id4001205- ud4000437 id4001882 63.4 4 id4003690-id4005389 id4004461 137.4 6 id6006336- id6007759 id6007312 44.6 6 id6010515- id6012967 id6011613 72.6 8 id8001604- id8002632 id8002098 30.1 8 id8006885- id8007977 id8006997 100.1 9 id9002357- id9003188 id9002721 37.7 9 id9004727- id9007259 id9004727 65.7 11 id11009197- id11011285 id11009456 102.9 12 id12006515- id12007988 id12007081 85.0
Non stress 2011 Add LOD R2 37.7 9.3 11.2 32.4 7.8 9.5 22.3 7.4 9.0 32.0 6.1 7.5 28.2 9.9 11.9 22.6 10.1 12.1 12.1 12.2 14.4 43.7 7.4 9.0 35.9 7.8 9.5 25.7 13.1 15.4 30.6 10.4 12.5 29.9 5.2 6.5
Non stress 2012 Add LOD R2 33.5 6.3 7.8 26.8 7.2 8.8 15.8 5.0 6.2 26.7 7.4 9.1 20.2 8.9 10.8 16.0 6.7 8.3 5.9 5.7 7.1 32.2 4.5 5.6 30.7 5.4 6.8 16.5 5.2 6.5 26.0 6.7 8.3 19.5 4.5 5.6
Combined Add LOD R2 35.7 10.6 12.8 29.8 10.2 12.2 19.2 8.3 10.1 29.5 8.2 10.0 24.5 13.0 15.4 19.5 10.7 12.8 9.2 12.3 14.6 38.3 8.0 9.8 33.4 8.8 10.8 21.4 12.3 14.6 28.4 11.8 14.1 25.0 6.6 8.2 S. Dixit, IRRI
High yielding BILs with QTLs affecting yield under non- stress
Line IR91648-B-7-B QTLs qDTY2.1, qDTY3.3, qDTY4.2, qDTY4.3, qDTY6.3, qDTY6.2, qDTY8.2, qDTY8.3, qDTY9.2, qDTY9.3, qDTY11.2, qDTY12.3 qDTY2.1, qDTY3.3, qDTY4.2, qDTY4.3, qDTY6.3, qDTY6.2, qDTY8.2, qDTY8.3, qDTY9.2, qDTY9.3, qDTY11.2, qDTY12.3 qDTY3.3, qDTY6.3, qDTY6.2, qDTY8.2, qDTY9.3, qDTY11.2, qDTY12.3 qDTY6.3, qDTY6.2, qDTY8.2, qDTY8.3, qDTY9.3, qDTY8.2 qDTY9.3 qDTY6.2 qDTY3.3, qDTY4.2, qDTY4.3, qDTY6.3, qDTY6.2, qDTY8.2, qDTY8.3, qDTY9.3, qDTY11.2, qDTY12.3 qDTY3.3, qDTY6.3, qDTY8.3, qDTY9.3, qDTY11.2, qDTY12.3 qDTY2.1, qDTY3.3, qDTY4.2, qDTY4.3, qDTY6.3, qDTY6.2, qDTY8.2, qDTY8.3, qDTY9.3, qDTY11.2, qDTY12.3 qDTY8.2, qDTY9.3,
DTF 81
HT % Swarna 120 72
IR91648-B-13-B IR91648-B-20-B IR91648-B-22-B IR91648-B-32-B IR91648-B-150-B IR91648-B-57-B IR91648-B-59-B IR91648-B-76-B IR91648-B-89-B IR91648-B-251-B MOROBEREKAN SWARNA
7161 7009 7909 7230 6237 6025 6323 6395 7020 7321 2234 4552
6434 6085 5518 4840 4826 5114 5341 4613 6592 4349 1171 4012
6792 6553 6714 6034 5529 5566 5835 5511 6811 5845 1701 4282
81 83 85 92 93 82 85 85 90 92 95 99
71 47 34 67 67 34 40 53 35 67
-B
-B
-B
-B
Way Rarem
Vandana
Apo
IR90020:22-283-B-4-B
Way Rarem
Vandana Apo
0
27 0.1
NF
70 NF
60
79 57
2667
2080 2267
0.01
0.09 0
1660
2127 2127
87
68 80
100
91 101
DTF 78 78 81 90
DTF 61 60 60 90
IR84894-83-15-481-B
Vandana
Pyramiding of major effect drought grain yield QTLs DTY1.1, DTY2.1 and DTY3.1 in Swarna Sub1:MAB
Pyramiding two QTLs
IR81896-B-195 X Swarna(WS2009) Fore ground selection Background selection (DTY2.1and DTY3.1) Fore ground selection Background selection
DTY1.1
DTY2.1 DTY3.1
1
2 3
RM11943-RM12091
RM327-RM262 RM520-RM16030
14
16 30
30
12.5 25
N22
Apo Apo
BC2 X Sub1Swarna(DS2010)
BC4F1 (DS2011)
BC4F2 (WS2011)
BC4F3 (DS2012)
Drought screening
BC4F2 (WS2011)
Select homozygote's
Swarna sub 1
BC4F3 (DS2012)
Drought screening
IR 94391-131-605-17
IR 94391-131-120-2 IR 94391-131-74-9 IR 94391-131-358-16 IR 94391-131-161-13 Swarna Sub1 Swarna
88
88 85 88 88 92 92
90
83 87 106 86 90 90
3731
3716 3714 3695 3686 2558 2120
6115
6582 6479 7907 6683 5207 4435
DTY3.1
DTY3.1 DTY3.1 DTY3.1 DTY3.1
DTY2.1
DTY2.1 DTY2.1 DTY2.1 DTY2.1
.
h h h s
s1
IR96321-1080-91
IR96321-327-107 IR96321-327-210 IR96321-1099-154
90
90
103
93
4479
4202 4022 3948
DTY1.1
DTY1.1 DTY1.1 DTY1.1
DTY3.1
DTY3.1 DTY3.1 DTY3.1
DTY2.1
DTY2.1 DTY2.1 DTY2.1
s
h s h
IR96321-1080-278
IR96321-967-412 IR96321-967-105 IR96321-1099-44 IR96321-967-57 IR96321-558-209 IR96321-1393-58 IR96321-678-240 IR96321-315-374
86
86 82 90 82 90 89
121
139 136 94 138 89 90
3939
3918 3909 3891 3885 3876 3855 3845 3836
DTY1.1
DTY1.1 DTY1.1 DTY1.1 DTY1.1 DTY1.1 DTY1.1 DTY1.1 DTY1.1
DTY3.1
DTY3.1 DTY3.1 DTY3.1 DTY3.1 DTY3.1 DTY3.1 DTY3.1 DTY3.1
DTY2.1
DTY2.1 DTY2.1 DTY2.1 DTY2.1 DTY2.1 DTY2.1 DTY2.1 DTY2.1
s
h s1 s1 s s1 s s s1
Apo
Swarna
76
96
115
92
2078
1896
IR94391-564-1-3
IR94391-707-1-1 IR94314-20-11-2 IR94313:1-9-2-1-1
75
75 78 78
102
100 93 107
3429
4238 5186 5767
5392
5343 5188 5059
IR94391-755-1-2
IR94313:16-37-1-3-4 IR94313:2-1-1-4-2 IR94314-20-9-3 IR94313:1-9-2-8-1 IR94313:18-4-1-5-2 IR94391-587-1-3 Apo Swarna Sub1
78
78 75 81 81 81 75 78 87
85
111 93 80 118 82 93 111 87
4098
3863 4634 5458 3895 4473 3662 6284 4789
5018
4989 4909 4868 4865 4863 4849 4263 1695
Swarna
91
88
4475
1895
DTF-NS
81 90 81 85 85 81 90 85
HTAVE-NS
89 95 99 95 97 93 102 93
GY-NS
6511 6464 6559 5967 7321 6478 10047 6783
GY -S
5817 5557 5354 5335 5271 5210 5093 5048
IR94313:19-7-1-4-1
IR88286-582-1-B-B-4-3 IR94313:8-1-1-4-1 IR94313:16-32-1-2-3
90
92 85 81
90
113 98 97
6544
10821 5055 4234
5029
4913 4898 4897
IR94391-303-1-1
Swarna Swarnasub1 Apo
85
95 93 78
101
92 95 110
10808
4867 5530 5574
4877
3048 3205 3386
Swamy, IRRI
MRQ74
X IR84984-83-15-18-B (qDTY12.1)
MRQ74
X IR81896-B-B-195 (qDTY3.1)
Foreground genotyping for qDTY3.1 X F1 (53) Foreground genotyping for qDTY2.2, qDTY 3.1 & qDTY12.1,
F1 (18)
743 (Total)
F1 (10)
MRQ74 Foreground genotyping for qDTY2.2, qDTY 3.1 & qDTY12.1, Recombinant & Full background genotyping
878 (Total)
35 (foreground genotyping)
BC1F1 (10) X Foreground genotyping for qDTY2.2, qDTY 3.1 & qDTY12.1, Recombinant & Full background genotyping
BC1F2 (5000) X Foreground genotyping for qDTY2.2, qDTY 3.1 & qDTY12.1, (homozygous condition), Recombinant & Full background genotyping, Selecting lines with different +qDTY combinations and -qDTY BC1F3 Field Screening (Dry season 2013)
Forward breeding
Currently at this stage
BC1F5 [5]
BC1F5 [5]
F1 [300]
Sabitri
Plants segregating for qDTY12.1 and qDTY3.2 with clearest background to be identified and back crossed to Sabitri
BC1F1 [1000]
Plants with qDTY12.1 and qDTY3.2 with clearest background to be identified and seeds multiplied
Plants segregating for qDTY12.1 and qDTY3.2 with clearest background identified and selfed
F3 [1000]
Seed multiplication and drought screening
BC1F3
Seed multiplication and drought screening
Mapping strategy
Development of Advanced Back Cross mapping population (BC2F2) Genotyping BC2F2 population Phenotyping BC2F3 and BC2F4 populations Identification novel major effect QTLs Introgression of QTLs in the recipient backgrounds
Swamy, IRRI
What we still do not know How the different combinations of QTLs work together in different genetic backgrounds In case of IR64, two QTL combinations with qDTY2.2 and qDTY4.1 were the best yielders
The identified QTLs have shown effect under drought in lowland and upland situations as well as for yield under normal situation, indicating their possible role in improving yield in puddled irrigated situation, dry direct seeded aerobic and dry direct seeded flooded situation (better water uptake, nutrient uptake ?)
Need to know the involved trait?
QTL Combinations
qDTY9.1, qDTY2.2, qDTY10.1, qDTY4.1 qDTY9.1, qDTY2.2, qDTY4.1 qDTY2.2, qDTY10.1, qDTY4.1 qDTY2.2, qDTY10.1, qDTY4.1 qDTY2.2, qDTY4.1 qDTY2.2, qDTY4.1 qDTY9.1, qDTY2.2 qDTY2.2, qDTY10.1 qDTY10.1, qDTY4.1 qDTY2.2 qDTY4.1 qDTY9.1 qDTY12.1 qDTY2.2 qDTY1.2 (S) qDTY1.1+ qDTY1.2 qDTY12.1+qDTY2.3 (P) qDTY12.1+qDTY3.2 (P) qDTY12.1 (P)+qDTY3.2 (P) qDTY12.1 +qDTY3.2 (S)+qDTY2.3 qDTY1.1+ qDTY1.2 +qDTY2.2 qDTY12.1 +qDTY3.2 (S)+qDTY2.3(S)
Background
IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64 IR64
BPM. Swamy, IRRI
QTL Combinations
qDTY1.1 qDTY2.2 qDTY2.3 qDTY3.2 qDTY12.1 qDTY2.3+qDTY2.3 qDTY2.2 +qDTY12.1 qDTY1.2+qDTY12.1 qDTY1.1+qDTY12.1 qDTY2.2 +qDTY2.3+qDTY12.1 qDTY1.1+ qDTY1.2 +qDTY2.2 qDTY1.1+ qDTY1.2 +qDTY2.3 qDTY1.1+ qDTY1.2 +qDTY12.1 qDTY1.1+ qDTY1.2 +qDTY12.1+qDTY2.2 qDTY1.1+ qDTY1.2 +qDTY2.2+qDTY2.3 qDTY1.1+ qDTY1.2 +qDTY12.1+qDTY2.3 qDTY2.2 +qDTY2.3+qDTY3.2+qDTY12.1
Background
IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1 IR64 Sub1
F1 Plants (S)
IR64 Sub1
(S): Segregating (P): Partial introgression
S. Dixit, IRRI
QTL combination qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2 qDTY1.1 + qDTY3.2
Background Swarna Swarna Swarna Swarna Swarna Swarna Swarna Swarna Swarna Swarna Swarna Swarna Swarna
Swarna Sub1
IR 91659:41-95-9
Swarna Sub1 Swarna Sub1 Swarna Sub1 Swarna Sub1 Swarna Sub1
P. Vikram, IRRI
NILs for DTYQTL Developed NILs shall help to Understand physiological and molecular mechanism involved with each QTL
Know the combinations of QTLs highly likely to provide higher grain yield under drought Facilitate MAB in different background without any undesirable linkage drag
Characterize individual QTLs/QTL combinations for their effect in different severity of stresses
Characterize different regions for severity of stress they are prone to.
2000-2004
2005-2008
2009 - 2013
large effect QTLs identified QTL pyramiding in popular rice mega varieties Fine mapping QTLs and candidate gene analysis MAS products successfully developed and tested in TPE Identification of novel QTLs from land races and wild species Marker aided recurrent selection (MARS) initiated
MARS and GWS fully implemented for better genetic gain Wild species derived QTLs used in MAS 2014 beyond QTLs pyramided and tested in hybrid parental backgrounds Genetic stocks developed, characterized, best combination of QTLs SNP chip developed, drought QTLs combined with other traits
DTY-QTL Recipient donors background DTY1.1 R X DTY3.1 R DTY3.2 R DTY6.1 R DTY12.1 R DTY2.3 R
qDTY3.1
F2
Lines with QTL homozygote selected
1 year
MAB Product
Conclusions
At least six QTLs- qDTY1.1, qDTY2.2 qDTY2.3, qDTY3.1, qDTY3.2, qDTY6.1 and qDTY12.1 have shown effect in more than one genetic backgrounds and across both lowland and upland ecosystem.
Their use in MAB have successfully shown yield increase of Vandana, IR64, Swarna, Swarna sub1 under drought. There are indications that these QTLs provide yield increase under normal situation, aerobic situation and can be useful to increase yield under dry direct seeded flooded situation. We do not know the best QTL combination for different backgrounds. Understanding the physiological and molecular mechanism of individual QTLs on NILs will help us to streamline this and make MAB easy. Developing SNP chip for these QTLs/QTLs combinations can help to make MAB quick, easy and help to integrate these QTLs into market segmentation breeding program of IRRI to combine with other traits.
Partners
Bangladesh BRRI, Gazipur RRS, Rajshahi India BAU, Sabour BAU, Ranchi BF, Hyderabad CRRI, Cuttack CRURRS, Hazaribag DRR, Hyderabad ICAR-NEH, Tripura ICAR, Patna IGAU, Raipur JNKVV, Jabalpur NDUAT, Faizabad OUAT, Bhubaneshwar TNAU, Coimbatore UAS, Bangalore Nepal NRRP, Hardinath RARS, Nepalganj RARS, Tarharra
Philippines PhilRice Laos NAFRI Mozambique-IIAM, Chokwe Tanzania DASRC, Morogoro Malaysia UKM and MARDI RDA, Korea
Donors
Rockefeller Foundation Bill and Melinda Gates Foundation Generation Challenge program Asian Development Bank DBT, India Devgen RDA, Korea GIZ, Germany Univ. Kebangsaan Malaysia, Bangi MARDI, Malaysia
Thank you very much for your kind attention and time