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M. Anitha Sri,Y11MPH448, I/II M. Pharmacy, II Semester, Industrial Pharmacy, Chalapathi Institute Of Pharmaceutical Sciences.
In-vitro Characterisation
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Drug Content In-vitro drug and Hb release Osmotic Fragility Osmotic Shock Turbulence Shock Morphology % Cell Recovery Entrapped Magnetite ESR ZSR
Drug Content:
Packed loaded cells (0.5mL)
Incubation of loaded normocytes at 37 0C in PBS (pH 7.4) at 50% haematocrit in a metabolic rotating wheel incubator.
0.8 spectropore membrane filter is fitted to a hypodermic syringe to withdraw the samples.
In-vitro Hb release:
5 % Hematocrit in PBS
Osmotic Fragility
Exposing to solutions of varying tonicities
Isotonic to hypotonic
Centrifuge Supernatant assayed for drug and Hb
Osmotic Fragility
Osmotic Shock
Sudden exposure to non-isotonic environment Incubation of resealed erythrocytes
Centrifugation
Supernatant assayed for Hb release
Turbulence shock
Measure of simulating destruction of loaded erythrocytes during injection
Loaded cells passed through hypodermic needle
Haemocytometer
Magnetite concentration
Magnetite loaded cells treated with HCl
NOVEL APPROACHES
Erythrosomes
Nanoerythrosomes
Biotinylated erythrocytes
Erythrosomes:
Erythrosomes are specially engineered vesicular systems in which chemically cross-linked human erythrocyte cytoskeletons are used as a support upon which a lipid bilayer (phosphatidyl choline)is coated.
Nanoerythrosomes
Normal erythrocytes
Depletion of Hb
Nanoerythrosomes
Biotinylated erythrocytes
Biotin molecules are attached to the amino groups present on the RBC. Methods: 1. Direct interaction. 2. Using spacer arm.
References:
1. S.P. Vyas, R. K. Khar, Targeted and controlled drug delivery, Novel carrier systems, Pg No. 387 to 416. 2. Advances in controlled and Novel Drug Delivery, N. K. Jain, Pg No. 332 to 360. 3. Controlled and Novel Drug Delivery, N. K. Jain, Pg No. 256 to 291. 4. Progress in controlled and Novel Drug Delivery Systems, N. K. Jain, Pg No. 387 to 416.