Monday Test 4.

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rDNA, pH

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A. B. C. D. Find the pH value of 0.05M HCL . 1.3 2.3 3.8 4 .9

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A. B. C. D. For an acid HA, the concentrations of HA and A- are 0.075 and 0.025 , respectively, at pH 6.0. What is the pKa value of HA? 6.4 7.4 8.4 5.4

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What is the change in H+ ion concentration , if pH is reduced from 5 3? A. 100 fold decrease B. 100 fold increase C. 20 fold decrease D. 20 fold increase

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In terms of Thermodynamic concepts , why it is more difficult to park a car in a small space than it is to drive it out from such space? A. Entropy increase is not favored reaction B. Decrease in Entropy and degree of freedom is favored C. Decrease in Enthalpy and space is not favored D. Driving in reverse is more difficult than driving front

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Aspirin is a weak acid with a pKa of 3.5. It is absorbed into the blood through the cells lining the stomach and the small intestine. Absorption requires passage through the plasma membrane, the rate of which is determined by the polarity of the molecule: charged and highly polar molecules pass slowly, whereas neutral hydrophobic ones pass rapidly. The pH of the stomach contents is about 1.5, and the pH of the contents of the small intestine is about 6. Is more aspirin absorbed into the bloodstream from the stomach or from the small intestine? A. Preferably in Stomach B. Preferably in Intestine C. Both in stomach as well as Intestine D. It is degraded in the low pH of stomach

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A. B. C. D. What is the H+ concentration of a solution with pH of 11.11 ? Approx 8 x 10-12 M Approx 4 x 10-12 M Approx 9 x 10-12 M Approx 8 x 10-10 M

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Pipe A can fill in 20 minutes and Pipe B in 30 mins and Pipe C can empty the same in 40 mins. If all of them work together, find the time taken to fill the tank A. 17 1/7 mins B. 20 mins C. none D. 50 mins

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Look at this series: 2, 6, 18, 54, ... What number should come next? A. 108 B. 148 C. 162 D. 216

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On sports day, if 30 children were made to stand in a column, 16 columns could be formed. If 24 children were made to stand in a column, how many columns could be formed? A. 20 B. 30 C. 40 D. 50

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A is two years older than B who is twice as old as C. If the total of the ages of A, B and C be 27, the how old is B? A. 7 B. 8 C. 9 D. 10

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A scientist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird). The researcher would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of dodo DNA available for testing? A. B. C. D. Polymerase chain reaction (PCR). electroporation gel electrophoresis Southern hybridization

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Which of the following tools of recombinant DNA technology is incorrectly paired with its use? A. restriction enzyme-production of RFLPs B. DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments C. DNA polymerase-used in a polymerase chain reaction to amplify sections of DNA D. reverse transcriptase-production of cDNA from mRNA

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A student wishes to clone a sequence of DNA of ~200 kb. Which vector would be appropriate? A. a plasmid B. a typical bacteriophage C. a BAC D. a plant virus

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A shuttle vector is a recombinant DNA vector capable of replicating in more than one bacterial species. Which of the following must a shuttle vector have? A. Pribnow boxes from both bacterial species. B. Shine-Dalgarno sequences from both bacterial species. C. Origins of replication from both bacterial species. D. Ribosomes from both bacterial species.

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A. B. C. D. Blotting describes Detecting the specific DNA fragment using probe The immobilization of sample nucleic acids on to a solid support Transfer of DNA from gel Staining the DNA using Ethidium Bromide

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Two purified DNA samples X and Y contain equal number of basepairs. Each of these DNA samples has one site each for EcoRI and BamHI restriction enzyme. Complete digestion with both the enzymes yielded 3 DNA bands and 2 DNA bands respectively for X and Y upon electrophoresis of the digestion products. Which one of the following explains the observation? A. X is circular and B is linear B. Y is circular and X is linear C. X is circular DNA and Y could be linear or circular D. Y is circular DNA and X could be linear or circular

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A gene that does not contain introns has an Sau3A site upstream of the translational termination codon in the 5' untranslated region. The size of the gene is 4 Kbp. The length of 5'UTR is 2 Kbp, the 3' UTR is 1 Kbp and that the open reading frame is 1 kbp. Upon probing a southern blot of Sau3A digested genomic DNA with the radioactively labeled fragment containing only the coding sequence, the size of the band detected by hybridization would be A. 1 Kbp B. >2 Kbp C. 0.5 Kbp D. 1.5 Kbp

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A linear 2000 base-pair (bp) long dsDNA is used as a template for PCR. One PCR primer is the same as the sequence at positions 101 to 125 in one strand of the template (numbering 5' to 3', starting with position 1). The other PCR primer is complementary to the sequence in positions 576 through 600 in the same DNA strand. What will be the length (in base pairs) of the amplicon? A. 50 B. 125 C. 451 D. 500

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When a PCR reaction is performed using genomic DNA as a template, a 1.5-kb product is amplified. When the same reaction is set up using cDNA as a template, a 0.8-kb product is amplified. A likely explanation for the different-sized products is that A. primers always bind to different sequences in different templates. B. there is a mutation in the genomic DNA. C. there is an intron in the gene. D. the cDNA is degraded

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Newly isolated restriction enzyme from Pseudomonas aeruginosa was used to digest the purified mouse genomic DNA. The restricted fragments were then separated and analyzed using 0.8% agarose gel, the average length of the restricted fragment was found to be 6,710,8864 bp. What is the most likely number of base pairs in the recognition sequence of the restriction enzyme isolated from Pseudomonas aeruginosa? A. 15 bases B. 13 bases C. 10 bases D. 11 bases

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Results
1 A
2 A 3 B 4 C 5 A

6
7 8 9

A
A C A

11 A
12 B 13 C 14 C 15 B

16
17 18 19 20

B
B D C B

10 D

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