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A double-edged sword
ROS/AOS
Reactive Oxygen Species (ROS) / Active Oxygen Species (AOS) Molecular oxygen is continually reduced within cells to several forms of Reactive Oxygen Species Produced during
Photosynthesis and respiration By-products of metabolism Dedicated enzymes
ROS-generating processes in chloroplasts, mitochondria, and peroxisomes Oxidation targets include proteins, DNA, and lipids
Reduction
Loss of oxygen Gain of hydrogen Gain of electrons
Oxidant
Oxidizes another chemical by taking electrons, hydrogen, or by adding oxygen
Reductant
Reduces another chemical by supplying electrons, hydrogen, or by removing oxygen
Non-Radicals:
Species that have strong oxidizing potential Species that favor the formation of strong oxidants (e.g., transition metals, H2O2)
Oxygen is normally reduced by four electrons to produce water Reaction catalysed by complex IV (cytochrome oxidase) of the mitochondrial electron transport chain Single electron transfer to molecular oxygen leads to ROS
Biologically important oxygen species
Superoxide radical anion Singlet Oxygen Hydrogen Peroxide Hydroxyl radicle
Light
e1O 2
O2 SOD
H2O2 APX
1 2
3 4
H2O
SOD types
Cu and ZnSOD
dimmer with subunit 32kDand each with one Cu2+ and Zn+mainly distributes in higher plant chloroplast mainly distributes in prokaryotic bacteria as well in eucaryotic mitochondria is a basic typemainly locates in green alga.
MnSOD
FeSOD
Transition metal catalyzed Other reductants can can suppliment Fe2+ like GSH, ascorbate, hydroquinones) Fe2+ is an extremely reactive oxidant
Redox balance
Cells maintain a particular redox balance. Antioxidant activity monitors ROS levels. The electronegativity of the cell is maintained by : reduced glutathione (GSH)
ROS generation
From electron transport and enzymatic sources (Chloroplast & Mitochondria) Glycollate oxidase
Photorespiration
Role of H2O2
Beta Oxidation
The reduced ferredoxin (Fd) is a major source of Superoxide radical Interior of thylakoid membranes (PETC)
Cellular antioxidants
Small Molecules Water soluble: glutathione, uric acid, ascorbate (Vit. C) Lipid soluble: -tocopherol (Vit. E), -carotene, coenzyme Q Proteins Intracellular: SOD (I and II), glutathione peroxidase, catalase Cell membrane: SOD (III), GPx, plasma proteins (e.g. albumin) Extracellular: phospholipid hydroperoxide GPx (PHGPx)
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