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Introduction of ATC
Animal Tissue Culture ? Roux in 1885 for the first time maintained embryonic chick cells in a cell culture Cell culture was first successfully undertaken by Ross Harrison in 1907.
Lewis (1911) - made the first liquid media consisted of sea water, serum, embryo extract, salts and peptones. Carrel (1913) - developed a method for maintaining cultures free from contamination. Rous and Jones (1916) trypsinization and subculture of explants. Eagle (1955) development of defined media. Littlefield (1964) - introduced the HAT medium for cell selection. Ham (1965) - introduced the first serum-free medium which was able to support the growth of some cells.
Harris and Watkins (1965) - were able to fuse human and mouse cells by the use of a virus.
2.Whether the cells are monolayer/suspension culture Monolayer culture plate Suspension culture Microtitration Flask
6.Cost
Interfare with the exchange of gas phase . To prevent foaming add antifoaming agent ex :Pluronic F68,CMC( carboxy methyl cellulose)
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