WELCOME PLANT TISSUE CULTURE

TISSUE CULTURE
TISSUE CULTURE IS THE METHOD OF PROPOGATION OF PLANTS IN LABORATORY CONDITIONS.

IT IS ALSO CALLED AS MICROPROPAGATION.

TISSUE CULTURING TECHNIQUE IS ADAPTED TO KEEP IN PACE WITH DEMAND AND QUALITY.

DIFFERENTIATION
The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is called dedifferentiation. The component cells of callus have the ability to form a whole plant, a phenomenon described as redifferentiation.

TOTIPOTENCY
THE CAPACITY TO GENERATE A WHOLE PLANT FROM ANY CELL OR AN EXPLANT IS TERMED AS TOTIPOTENCY.

EXPLANTS
A SMALL PIECE OF THE DESIRABLE PLANT IS SELECTED.

GENERALLY MERISTEMATIC TISSUE OR INTERNODAL SEGMENTS OF THE PLANT IS SELECTED FOR MICROPROPAGATION.

THE SELECTED PLANT TISSUE IS CALLED AS EXPLANT.

CULTURE

MEDIUM
THE TISSUE SO COLLECTED IS PLACED IN A CULTURE MEDIUM / NUTRIENT MEDIUM FOR THE MULTIPLICATION OF CELLS.

CONTENTS OF THE CULTURE MEDIUM:AGAR GEL.

A CARBON SOURSE SUCROSE.

INORGANIC SALTS . PLANT GROWTH REGULATORS AUXINS & GIBBERLLINS.

TISSUE CULTURE PLATES

CALLUS
THE CULTURED TISSUE WILL GROW INTO AN UNORGANISED MASS CALLED AS CALLUS . AS THE CALLUS GROWS THE NUTRIENTS IN THE MEDIUM STARTS DEPLETING. THE CALLUS SHOULD BE PETRIODICALLY CHANGED INTO NEW CULTURE MEDIUM FOR HEALTHY GROWTH OF THE PLANTS.

CALLUS

PLANTLETS GROWING
THE CALLUS SO GROWN AFTER DIFFERENTIATION IS TRANSFERRED

INTO SMALL CONTAINERS FOR FUTHER DIFFERENTIATION. NUTRIENTS AND PLANT HORMONES ARE ADDED FOR HEALTHY GROWTH.

A TISSUE CULTURE LAB

THE LABORATORY CONDITIONS SHOULD BE COMPLETELY ASEPTIC, HIGHLY STERALISED. THE EXPLANT SELECTED SHOULD BE STERALISED BEFORE PROPAGATION.

SOMACLONES
EACH OF THESE PLANTLETS WILL BE GENETICALLY IDENTICAL TO THE

PARENT PLANT FROM WHICH THEY ARE GROWN i.e. THEY ARE SOMACLONES.

STEPS IN TISSUE CULTURE

HARDENING
THE PLANTS ARE TRANSPLANTED TO THE LABORATORY AGRI-LAND FOR BETTER ADAPTABILITY BEFORE TRANFERING INTO THE MAIN FIELD.

THANK YOU