Experiment #1: Use of the Micropipettor and Spectrophotometer Results

Group 2 Francisco Bolinao IV David Angelo Guanzon Alfred Jerald Salvador Joseph Carlo Vergel 4BIO3

Testing Accuracy of Operators (Spectrophotometer)

Table 1 shows the concentration per absorbance reading

Absorbance Concentration

0.0210
0.0270 0.0400 0.0530 0.0580

6.2469 x 10-4
1.2480 x 10-3 1.8722 x 10-3 2.4950 x 10-3 3.1172 x 10-3

Absorbance vs. Concentration

0.07 0.06 0.05 Absorbance 0.04 0.03 0.02 0.01 0 0.0006249 0.001248 0.0018722 0.002495 0.0031172 Concentratio n

 Correlation

Coefficient:

0.988834 Regression Equation: y = a + bx y = 0.0058 + 19.2545 x

Testing Precision of Operators (Spectrophotometer)

Table 2 shows the absorbance readings per student, SD, mean, and CV.
Salvador Vergel Guanzon Bolinao 0.0660 0.0710 0.0600 0.0580

Standard Deviation Mean Coefficent of Variation

0.0059 0.0638 0.0927

B.1 Weight Determination of Water

Table 3 shows the weight of water per given volume.

Volume (uL) 5 10 30 50 70 Weight (g) 0.0047 0.0098 0.0299 0.0497 0.0699

Weight Determination of water

0.08 0.07 0.06 Weight (g) 0.05 0.04 0.03 0.02 0.01 0 0 20 40 Volume (uL) 60 80

B.2 Weight of 20uL water (c/o Mr. Alfred Salvador)

Table 4 shows the mean and standard deviation from weight determination of water.

Mean Standard

0.0219

Deviation

0.0047

Table 5 shows the mean and standard deviation from weight determination of water per student.
Standard Student Bolinao

Mean
0.0191

Deviation
0.0007

Guanzon
Salvador

0.0195
0.0200

0.0019
0.0017

Vergel

0.0192

0.0010