Professional Documents
Culture Documents
January 2009
DENTISTS BRAND DAY1 DAY4 DAY8 DAY11 DAY15 DAY18 DAY22 DAY25
BASCIANO Valiant 9.921 9.677 9.580 9.463 8.700 8.873 9.392 9.311
ECCLES Dispersalloy 9.966 9.620 10.851 10.590 11.260 9.070 9.280 9.014
FISCHER Valiant 5.958 5.829 4.408 4.533 4.266 4.473 5.136 4.460
GRUBE Valiant 6.841 6.904 6.788 5.782 8.158 7.740 7.893 8.026
MESSERMAN Dispersalloy 11.357 11.238 11.887 12.086 15.335 14.712 14.473 15.859
GIRLS
BOYS
Membrane
Bound Organelle
Axon Dynien
Microtubule
Kinesin
Only Hg2+ Induces Aberrant [32P]8N3GTP-ß-
Tubulin Interactions In Normal Brain Mimicking
the Observation seen in AD Brain
120
Placing amalgams in water makes it toxic
100 to brain tubulin just like adding Hg2+.
80
% Active
Tubulin 60
40
20
0
l
1
o2
o4
o8
12 2
24 4
72 2
48 8
96 96
tro
0
o1
12
o
0t
1t
2t
4t
to
to
to
to
n
8t
to
co
MOST VACCINES
CONTAIN TRACES OF
THIMEROSAL EVEN IF IT
IS NOT ADDED AS A
PRESERVATIVE.
100
80
Control
50 nM thimerosal
50 nM thimerosal
60 +10 nM HgCl2
50 nM thimerosal
+ 25 nM HgCl2
40 10 nM HgCl2
25 nM HgCl2
20
0 5 10 15 20 25 30
1.75 µg Neomycin/ml
80 50 nM Thimerosal
500 nM Al(OH)3
50 nM Thimerosal
60 50 NANOMOLAR 1.75 µg Neomycin/ml
50 nM Thimerosal
THIMEROSAL 500 nM Al(OH)3
40 1.75 µg Neomycin/ml
DR. MARK
20
LOVELL
COLLABORATOR
+ TESTOSTERONE
0
0 5 10 15 20 25 30
14
12
10
Hair Hg level
(mcg/g) 8
Data from A. Holmes,
M. Blaxill & B. Haley,
6 Controls
Int. J. of Toxicology
v22, 2003
4
2
Autistic
0
Number of amalgams: 0-3 4-5 6-7 8-9 >10
Control: autistic ratio: 2.64 6.93 6.70 6.32 17.91
N: 15 22 29 30 43
Other Epidemiological Studies
• A study on seven-year-old children in the Faeroe Islands
found that blood pressure problems increased with
decreased blood Hg. This implies retention toxicity effects
of Hg in this comparison.
• In the Sechylles study of >700 children, boys with higher
levels of hair mercury performed better on some tests as the
Boston Naming test. This implies that ability to excrete
increases hair Hg levels, not exposure, in this comparison.
• Healthier children seem to be more exposed to mercury if
one believes high blood and hair Hg are measure of
exposure.
• CONCLUSION: Blood and hair Hg levels are not a measure
of exposure at low levels, but rather a measure of both
exposure and ability to excrete mercury.
The involvement of the 2004 Institute of
Medicine (IOM) report.
• The 2004 IOM committee was funded by the CDC.
• The 2004 IOM report cleared thimerosal as being involved
in autism and recommended that no further research be
done on this issue but to investigate other more fruitful
areas like genetics—which has failed to find a significant
genetic component of autism.
• The 2004 IOM report was based only on 5 epidemiological
studies of questionable value, none lead by an American.
• The 2004 IOM report totally dismissed the basic science
research on thimerosal toxicity and the resultant aberrant
biochemistry possibly caused by mercury-like toxicity
reported by several research scientists.
• A congressionally requested NIH committee looked at
the 2004 IOM report and gave it a very bad evaluation.
• The CDC is living in a state of denial!
Who did the Epidemiological Studies the IOM depended on??
• The Verstraten (Belgium) studies at first showed autism rates were
enhanced by thimerosal exposure but changed with renditions to show
no effect. All the CDC data was lost or destroyed after it was
published. Verstraten now works for a major vaccine producer in
Europe.
• Two studies were done by Danish (Madsen and Hviid) who worked
for the Stantens Serum Institute (SSI). SSI makes thimerosal
containing vaccines and sells them to other countries because they are
not allowed to be used in Denmark since 1992. These studies showed
a 20-fold increase in autism on removal of thimerosal! STUPID!
• One study was done in England by E. Miller. After her results were
made known at the 20004 IOM meeting the National Health Service
removed thimerosal from English vaccines.
• Troubling, that the opinion of the CDC is based totally on foreign,
conflicted opinions. Why couldn’t the CDC find epidemiologists in
the USA to do these studies???
• The Verstraten studies differed from the Danish and English study in
that it did not show the dramatic protection effects of thimerosal
against autism!!!!!
Autism Risks From 5 Sequential Studies by
Verstraten et al. of CDC
Study1 Study2 Study3 Study4 Study5
Ischiropoulos H, Beckman JS. Oxidative stress and nitration in neurodegeneration: Cause, effect or association? J Clin
Invest.
2003;111:163–169.
Kern JK, Jones AM. Evidence of toxicity, oxidative stress and neuronal insult in autism. J Toxicol Environ Health B Crit
Rev. 2006;9:485–499.
Larsson HJ, Eaton WW, Madsen KM, et al. Risk factors for autism: Perinatal factors, parental psychiatric history and
socioeconomic status. Am J Epidemiol. 2005;101:916–925.
Deth R, Muratore C, Benzercry J, et al. How environmental and genetic factors combine to cause autism: A
REACTIVE OXYGEN SPECIES (ROSs)
• Superoxide Anion: O2 + e- O2-.
(Fenton)
2O2-. + 2H+ H2O2 + O2 (keeps O2-.
The hydroxyl radical is the most damaging!
<10-11M)
Both O2-. radical and the quinone radical can leak from damaged
mitochondrial membranes during the electron transport process
involved in making ATP requiring GSH to scavenge them decreasing
Structures and General
Chemistry of Glutathione
GOOD
BAD
reduced oxidized
oxidized reduced
REVERSAL OF LIPID PEROXIDATION BY
GLUTATHIONE PEROXIDASE (GP)
R = CH3CH=CH-CH=(CH)n-COOH (a poly-unsaturated
fatty acid, pufa)
MITOCHONDRIAL GSSG IS
INCREASING AND GSH IS
DECREASING WITH CELL
DEATH.
H2 O2
LEVELS
O O O O
N
NH HN NH HN
Free radical scavenging sites
SH HS SH HS
Potent scavengers of hydroxyl radicals in lipophilic areas.
O NH O O HN O
HO OH
S NH HN S
NH S S NH
NH2 H2N
O O O O O O
O HO H3C CH3 OH O
Glutathione Glutathione
Very water soluble
AN OXYGEN RADICAL ABSORBANCE STUDY OF ONE OF THE NEW
ANTIOXIDANTS DONE IN AQUEOUS SOLUTION.
60
y = 0.1487x + 3.0744
50
R2 = 0.9994
40
Trolox Eq uM
30
20
10
0
0 50 100 150 200 250 300 350
CT1 uM
Toxicity Study of Lipid Soluble Antioxidant
• Group A B C D
• Test 1 0 100 200 300
• Test 2 0 200 300 400
• Test 3 0 300 400 500
• Total 0 600 900 1,200