Professional Documents
Culture Documents
Engineering: Preparation,
Characterization, Modeling
Guide
Dr. Murali Rangarajan
Co-Guide
Dr. Nikhil K Kothurkar
Polymeric Scaffolds
PU Scaffolds
Synthesis: Solid/Liquid Phase Separation/Freeze
Drying
Characterization: Density and Porosity Measurements,
P P
LLA U
Characterization of Scaffolds
Porous PLLA and PU scaffolds at 200X magnification
characterized by optical microscopy
P P
LLA U
Inferences
n Both PLLA and PU scaffolds are highly porous
None of these steps will proceed if the cells do not adhere well
to the scaffold
Cell adhesion
n Cell adhesion is the contact and firm interaction between
cells, between cells and extra cellular matrix (ECM) and
between cells and synthetic materials such as scaffolds in
tissue engineering
Dong Kong, Baohua Ji, Lanhong Dai, 2008. Nonlinear mechanical model
of cell adhesion, Journal of Theoretical Biology 250, 75-84
Linear Peeling model
n Linear, where the cell detachment occurs when
the bond force at the leading edge of the cell
exceeds the maximum bond force
Force is assumed to be
a linear
Evan A. Evans, 1985. Detailed
function of the mechanism of membrane- memb
displacement of adhesion and separation,
bond length (ζ) from Biophysical Journal, 48, 175-183
Governing Equations
Adhesive Free
Zone Zone
Free
Zone
Relationship between δ
and θα ratio of adhesive
energy(γ) to bending
modulus (B)
Results obtained for shape of the
cell
Inferences
Where x > 0
a = 0.8107 ± 0.192 ; b = 2.147 ± 0.303
The above equation can rewritten
in terms of force f, and displacement ζ as