Professional Documents
Culture Documents
Microbial System To achieve homogeneity of chemical environment in the presence of nutrient sink and product source terms To meet oxygen supply requirements of the biomass, often limiting in scale up
Plant Cell System To achieve homogeneity of biomass distribution in the presence of sedimentation due to large size of cell aggregates To meet oxygen requirement of the growing biomass without depleting the concentration of gaseous products below a critical level Usually very important due to large size of cells and aggregates.
Oxygen transfer
Shear
Usually unimportant.
Mammalian Cells
Secondary cells
After the first passage of primary cells Have a finite number of passages
Suspension cells
Cells not requiring any surface attachment Examples are: HeLa cells, hybridoma cells and other tumor cells
Special Modifications
Modes of Operation
Batch mode of operation
A
well established production method; generally used for vaccines The operation modes all lack in the ability to and biopharmaceuticals.
Stirred-Tank Reactor
Low
yield of product
Batch
Fed-batch mode
High
density culture operation and higher yield of dead cells and debris
Continuous mode
Feed
Longer
Accumulation
Fed-batch
Feed
High
density culture
Product
Harvest
dilution rate
Continuous
Perfusion Culture
Perfusion mode High density culture Product can be harvested continuously High productivity Dilution rate not limited Separation of viable and dead cells possible
Volume of the production unit is smaller than normally required with batch culture. Lower investment cost on the bioreactor.
2. Centrifugation
3. Gravitational settling 4. Immobilisation
Liquid addition
Spin filter bioreactors Cell settler for perfusion culture Centrifugal bioreactor Tangential flow or crossflow filtratio device for perfusion culture Packed-bed basket perfusion culture
Spent medium
Draft tube