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History of Peroxisomes
First
observed by electron microscopy in animal cells (1950s), then in plant cells (1960s)
Christian
deDuve (1965)
Isolated from liver cells by centrifugation Called them peroxisomes because they generate and destroy H2O2
The Peroxisome
Single
0.2 - 1.7m
Composition
varies
Glucose limited
Methanol limited
Protein Import
C-terminal signal sequence: SKL N-terminal signal sequence: RLX5HL Proteins involved in import: peroxins Import driven by ATP hydrolysis Dont have to be unfolded for import
2.
3. 4. 5.
-oxidation of fatty acids Glyoxylate cycle Photorespiration (Glycolate pathway) Degradation of purines Decomposition of hydrogen peroxide
Germinating
seeds
Converts fatty acid in seed lipids into sugars needed for growth in the young plant
Glyoxysomes
-oxidation occurs in mitochondria and peroxisomes in mammals, but exclusively in the peroxisome in plants and yeast.
Greening cotelydons
The same population of peroxisomes assumes different metabolic roles depending on developmental stage of cotelydon
Glycolate cycle
Production of CO2 Involves 3 organelles (chloroplasts, peroxisomes, & mitochondria)
H2O2 production
Purine Degradation
Nucleic
acid purine moieties (adenine and guanine) are degraded to uric acid
O2 H2O2 O2 H2O2
xanthine
uric acid
Urate oxidase
allantoin
Xanthine oxidase
Urate Oxidase
High urate oxidase concentrations contribute to formation of crystalline inclusions All purine degradation leads to uric acid
H2O2 O2
O H N C N C H N H N H
H2O2
O2
O H N C
purines
H N H C N
N C H N H
FAD
FADH 2
O
FAD FADH 2
O
H N C O
N H
N H
hypoxanthine
xanthine
uric acid
Oxidases
The oxidases use molecular oxygen to remove hydrogen atoms from specific organic substrates A variety of compounds, including L-amino acids, D-amino acids, polyamines, methanol, urate, xanthine, and very-long-chain fatty acids, serve as substrates for the different oxidases
Peroxide Detoxification
Oxidases use O2 to oxidize organic substances and produce hydrogen peroxide (H2O2) -- e.g., H2O2 generated by glycolate oxidase reaction, -oxidation of fatty acids
Peroxisomes also contain catalase, the enzyme that degrades H2O2.
Other
.. . .. . . .. .. .. .. - .. O : H H : O :H : : : : : : : :O : : : + O O O H O O H H O . . . .. .. . .. . . .. ..
Superoxide Hydrogen Hydroxyl anion peroxide radical (radical) Cause damage to lipids, proteins, DNA Amount ROS is reduced by catalase, and superoxide dismutase (SOD) 2O2O2 + H2O2
Radical Chemistry
Initiation: RH + O2 -->R + OH
Termination: R + R --> RR R + ROO--> ROOR ROO + ROO --> ROOR + O2
Conclusions
Compartmentalize! To protect the cell from these destructive byproducts, such reactions are segregated.
Peroxisomal Diseases
Adrenoleukodystrophy: Deficiency in oxidation of very longchain fatty acids