Professional Documents
Culture Documents
OF GASES
prePARed by
doctoroid
ON
• One of the major events in
the process of ‘External
respiration’.
• ‘Haem’ is an iron-
porphyrin compound,
where iron is present
in the ‘ferrous (Fe++ )’-
form & is the site of
oxygen-binding.
More About Hb
• The ferrous O2 binding sites in Hb also bind
NO, & an additional NO binding site is
present on the beta chains. The affinity of
this 2nd site is increased by O2, so Hb binds
NO in the lungs and releases it in the
tissues, where it promotes vasodilation.
functions of Hb:
• it facilitates O2 & CO2 transport;
• has an important role as a buffer ;
• it transports NO.
Oxygenation of Hb:
• most of the O2 quickly diffuses from
the plasma into the RBCs
• combines with Hb in a loose & readily
reversible manner---rapidly
(<0.01sec)
• occupies the 6th coordination-position
of the iron atom & does not become
ionic oxygen .
• accompanied by a conformational
conversion in the Hb-molecule: deoxy-
hb(T) oxy-hb(R)
Reaction of Haem with Oxygen
(M, V, and P stand for the groups shown on the molecule on the left)
Contd…….
One O2-molecule combines with the ‘(Fe )’- of each
++
Comparison with
OHDC:
•Rectangular Hyperbola
•Takes up O2 at lower
PO2 , much readily.
•Does not show Bohr’s
effect.
•Hb-affinity of CO is ~200
Hb(HbCO)
times than that of O2
•P50 value of CO= only 0.5
mm Hg
•CO binds with Hb at the
same site (as of O2 ) .
•Interferes with O2 –
transport by decreasing
functional Hb-conc.
•in the presence of CO as a
modifying factor, OHDC
Shifts to left as binding of
CO causes a conformational
change in the Hb causing
increased affinity for O2 by
the other subunits.
tissues:
•O2 diffuses rapidly first from the
peripheral capillary blood to the ISF
along a PG of 95-40=55 mm Hg &
then from the ISF into the tissue-cells
along approx. PG of 40-23= 17 mm
Hg.
•Tissue Po2 is determined by a
balance b/w (i)rate of O2 -transport to
the tissues by the blood & (ii) rate at
which the O2 is used by the tissues.
• Only 1- 3 mm Hg of PO2 is normally
required for the oxidative chemical
processes in the cell. So this low
intracellular PO2 is more than
adequate and provides a large safety
factor.
CO2 TRANSPORT
From tissues to the lungs.
Acc. to the differences in PCO2 at
various sites:
• Intracellular PCO2 46 mm Hg
• Interstitial Fluid PCO2 45 mm Hg
• Arterial blood(at the tissue
capillaries)PCO2 40 mm Hg
• Venous blood PCO2 45 mm Hg
Alveolar Air P 40 mm Hg
3 major
steps
• A) Uptake of CO2 by the
blood
• B) Transport of CO2 in the
blood
• C) Release of CO2 in the
lungs
A) Uptake of CO2 by the blood:
•from the cells rapidly
diffuse to ISF even if the PG
is only 46-45=1 mm Hg
•From the ISF, diffuse into
the capillary blood(which
flows in the systemic
venous system)along a PG
of 45-40=5 mm Hg.
•Diffusivity of CO2 is ~20
times higher than O2 .
B) Transport of CO2 in the
blood:
In 3 forms—
• I) Dissolved form (7%)
• II) As Carbamino compounds (23%)
• III) As Bicarbonate (70%)
Normal CO2 content (per 100 ml blood)--
• Venous ~ 52 ml & Arterial ~ 48 ml
So, ~4ml of CO2 is transported per 100ml of
blood from tissue cells to lungs .
I. CO2 TRANSPORT IN
DISSOLVED FORM:
• Obeys Henry’s law.
• Venous blood(at PCO2 =45mm Hg)
& Arterial blood(at PCO2 = 40 mm
Hg) contain respectively 2.7 vol %
& 2.4 vol % of CO2 in dissolved
state.
Only 0.3 ml of CO2 is transported
II. CO2 TRANSPORT AS
CARBAMINO COMPOUND:
• After entering the blood, some CO combines with the
2
(-NH2) of proteins to form ‘carbamino-compounds’
In the Plasma: combines with plasma-proteins—
CO2 + Pr.NH2 Pr.NH.COOH (relatively insignificant)
• HCO 3
-
diffuse out into the plasma & transported as Sod.bicarbonate
• H are buffered by deoxygenated Hb(weaker acid than oxygenated Hb)
+
•D/t constant
ventilation this CO2 is
then transported to
the atmosphere.
Schematic Representation of blood transport & release of CO2
in Lungs
Outline of summary of the Blood Gas Transport
Gas Content of
Blood
2 Special Related
Terminologies
Utilization Co-efficient: Respiratory Quotient:
• percentage of the ratio • Ratio of rate of CO2
of O2 consumption
excretion(N=4ml/1
rate(N=250 ml/min) &
O2 -delivery rate
00 ml) & rate of O2
(N=1000ml/min) in the consumption(N=5
tissue. ml/100 ml) per
min.
Normal avg. UC =
250/1000 X 100% = 25 Normal avg. RQ =
%
4/5= 0.8
Till Death.........
SOME
PONDERABLE
FACTS
Think before You Move…
Combination oxygen with
Hb is called ‘oxygenation’
but not oxidation.
Almost flat bottom portion of
the initial OHDC is a safety
measure, esp for persons with
Chronic lung disease.
Stored blood(esp with ACD as
anticoagulant) is not safe to be
transfused in a severely
hypoxic patient.
The high RBC count in
foetus is basically d/t
the characteristic ODC
of HbF.
The Hct value of venous
blood is about 3% higher
than that of arterial
blood.
Hypoxemia occur much
earlier than hypercapnia in
patients with ‘Diffusion-
defects of lungs’.
Hyperbaric oxygen is
therapeutically utilised
in CO poisoning.
The point on OHDC
representing PO2 = 60 mm
Hg & SO2 %=90, is called
the ‘ICU Point’.
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