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Course Contents
Differences between batch and continuous system based on productivity, modifications for batch and continuous bioreactor, simple bioreactor design for immobilized cell systems, animal and plant cell cultures, solid state fermentation process.
Chapter 9
Bioprocess Engineering Basic Concepts Michael L. Shuler/Fikret Kargi Prentice Hall International Series in the Physical and Chemical Engineering Sciences
INTRODUCTION
The choice of reactor will determined: Product concentrations and purity Degree of substrate conversion/yield Whether sustainable/reliable performance
The bioreactor consideration must be made in the context of the total process: Biocatalyst Reactor Separation Purification
tc
Xm
Xm ln t1 X0
X0
X m X 0 YX S S 0
rb
1 m ln X m
YX S S0
X 0 t1
Chemostat
Operated by supplying an essential growthlimiting nutrient at a constant rate with the result that the culture density and growth rate adjust themselves to the supply.
Turbidostat
Operated by maintaining a constant cell density by supplying fresh medium as required. It possesses a photocell system which continuously monitors the cell density and maintains this constant by controlling the medium inlet flowrate.