This document provides setpoint values and operating parameters for various batching steps and process instrumentation in a bioprocessing facility. It details setpoints for pressure testing, sterilization, and individual batch steps. It also explains the basic principles of pH, temperature, pressure, level, and dissolved oxygen transmitters. pH transmitters measure potential differences related to hydrogen ion concentration. Temperature transmitters use thermocouples to detect voltage changes from temperature differences. Pressure transmitters convert pressure into electrical signals using transducers. Level transmitters measure capacitance that depends on material inside a tank. Dissolved oxygen transmitters use a probe membrane to measure diffusion rates proportional to oxygen levels.
Original Description:
This PPT describes briefly about instruments used widely in bioreactors and its common setpoints.
This document provides setpoint values and operating parameters for various batching steps and process instrumentation in a bioprocessing facility. It details setpoints for pressure testing, sterilization, and individual batch steps. It also explains the basic principles of pH, temperature, pressure, level, and dissolved oxygen transmitters. pH transmitters measure potential differences related to hydrogen ion concentration. Temperature transmitters use thermocouples to detect voltage changes from temperature differences. Pressure transmitters convert pressure into electrical signals using transducers. Level transmitters measure capacitance that depends on material inside a tank. Dissolved oxygen transmitters use a probe membrane to measure diffusion rates proportional to oxygen levels.
This document provides setpoint values and operating parameters for various batching steps and process instrumentation in a bioprocessing facility. It details setpoints for pressure testing, sterilization, and individual batch steps. It also explains the basic principles of pH, temperature, pressure, level, and dissolved oxygen transmitters. pH transmitters measure potential differences related to hydrogen ion concentration. Temperature transmitters use thermocouples to detect voltage changes from temperature differences. Pressure transmitters convert pressure into electrical signals using transducers. Level transmitters measure capacitance that depends on material inside a tank. Dissolved oxygen transmitters use a probe membrane to measure diffusion rates proportional to oxygen levels.
Manager: Mr Gokul Jothiraman Setpoints of Individual Steps Batching steps that require setpoints Pressure tests Sterilization-in-Place (SIP) Empty SIP Full-Vessel SIP Media Sterilization Disinfection Pressure Test Initial Pressure Test, Vessel Pressure Test I (VPI) & Vessel Pressure II (VP II)
Test SP 1.40 bar Test Time 30 min Dep SP 0.1 bar Air Flow SP 30 LPM Test Hyst 0.1 bar Sterilization ESIP and FSIP
Heating 1 90 C Heating 2 121 C Cooling 1 90 C Cooling 2 30 C Holding Time 1 (FSIP) 30 min Total Holding Time (FSIP) 60 min Sterilisation Time (ESIP) 60 min Back Pressure SP 1.3 bar Air Flow SP 30 LPM Dep SP (ESIP) 0.1 bar Heating Hyst 0.1 C pH, Temperature, Pressure and Level Transmitter Basic Principles of Operation pH Transmitter Probe consists mainly of measuring electrode and reference electrode The measuring electrode develops a potential (voltage) directly related to the hydrogen ion concentration of the solution. The reference electrode provides a stable potential against which the measuring electrode can be compared. Both electrode contain KCL buffer solution that has constant H +
concentration pH Transmitter (contd) If: H + conc. inside solution > H + conc. inside electrode Potential difference = Positive Acidic H + conc. inside solution < H + conc. inside electrode Potential difference = Negative Basic H + conc. inside solution = H + conc. inside electrode Potential difference = Zero Neutral
Temperature Transmitter Using a thermocouple consists of two wires of dissimilar metals or alloys joined at their ends, with a potentiometer (or a voltmeter) connected in one side of the circuit. Measuring junction Reference junction measuring junction in the environment whose temperature is to be measured. reference junction kept at a fixed, known temperature
Temperature Transmitter (contd) Works by the difference in the conductivities of the two wire materials at a measured temperature Difference of the temperature between the measuring junction and reference junction will create an electric current Increase in temperature increase voltage Decrease in temperature decrease voltage Temperature of the medium can be determined from the current voltage strength.
Pressure Transmitter Via a pressure transducer device which converts an applied pressure into a measurable electrical signal 2 main parts elastic material which will deform when exposed to a pressurized medium (diaphragm) a electrical device which detects the deformation. Pressure Transmitter (contd) Types of transducers: Resistive pressure transducer strain gauges bonded to the surface of the diaphragm any change in pressure will cause a change in the electrical resistance of each strain gauge
Variable capacitance pressure transducer capacitive plate bonded to one side of the diaphragm and another capacitive plate bonded to an unpressurized surface.
Level Transmitter Using principal of RF signal applied to capacitance circuit An insulated electrode acts as one plate of capacitor and the tank wall acts as the other plate. Capacitance is dependent on the amount of product inside the tank: an empty tank has lower capacitance a filled tank has higher capacitance. Level Transmitter (contd) Measures by applying an RF signal between the conductive probe and the vessel wall. The RF signal results in a very low current flow through the dielectric process material in the tank from the probe to the vessel wall. When the level in the tank drops/increase, the dielectric constant drops/increase causing a drop/increase in the capacitance reading. The capacitance reading will then be translated into a certain level unit.
DO Transmitter Probe has thin organic membrane covering a layer of electrolyte and two metal electrodes. Oxygen diffuses through the membrane at a rate proportional to its partial pressure Higher oxygen partial pressure will increase the diffusion rate Oxygen is reduced at the cathode and the current is measured converts measured current into concentration units. Diffusion current is directly proportional to the concentration of dissolved oxygen References http://www.engineersgarage.com/articles/pressure- sensors-types-working?page=2 http://www.sensorsone.co.uk/pressure-measurement- glossary/pressure-transducer.html http://www.sensorland.com/HowPage037.html http://enggyd.blogspot.in/2011/01/ph.html http://coep.vlab.co.in/?sub=33&brch=91&sim=449&cn t=1 http://www.endress.com/eh/home.nsf/#products/~le vel-measurement-principle-capacitive http://www.smartec.nl/pdf/SPDprinciples.pdf http://en- co.wika.de/upload/OI_A_10_en_de_fr_es_6240.pdf https://controls.engin.umich.edu/wiki/index.php/Temp eratureSensors