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Hemostasis
Coagulation
Fibrinolysis
Hemostasis
Definition:
The process the body uses to
stop the flow of blood when the
vascular system is damaged.
I Fibrinogen 纤维蛋 72 - 96
白原
II Prothrombin 凝血 60
酶原
III Tissue Factor or -
thromboplastin 组织
因子
IV Ca++ -
V Proaccelerin 前加 15
速素
VII Proconvertin 前转变 5
素
Factor Name Plasma
half-life (h)
IX Antihemophilic B factor or 25
Christmas factor 血浆凝血激
酶
X Stuart or Stuart-Prower 40
factor
XI Plasma thomboplastin 45-65
antecedent 血浆凝血激酶前质子
Procoagulants
More than 50 kinds in blood
Anticoagulants
Whether blood will coagulate depends on
the balance between Procoagulants and
Anticoagulants.
In the blood stream, the anticoagulants
normally predominate.
The reasons that blood can flow
through vessels fluently are as
follows:
There is no injury in blood vessel.
Most of clotting factors are present
in blood with inactive state.
Thereare some anticoagulants
(Compounds that do not allow
blood to clot) exist in blood.
Anticoagulative factors
FVa, FVIIIa
activate inactivate
Anticoagulative factors
2. Antithrombin action of fibrin and
antithrombin III.
Both work by removing thrombin.
(1) fibrin fibers adsorb thrombin
(2) antithrombin III (an alpha-globulin,
produced in liver and endothelial
cells) combine with unadsorbed
thrombin and then inactive it.
Anticoagulative factors
3. Heparin.
(1) It combines with antithrombin III,
then the effectiveness of antithrombin III
in removing thrombin increases by a
hundredfold to a thousandfold.
(2) The complex also removes Xa, XIa,
IXa and FXIIa.
Heparin forms a high-
affinity complex with
antithrombin (AT-H).
X Xa TFPI
II IIa
Catalysis Convert
Clot retraction---serum
Serum:
Definition
Within a few minutes after a clot is
formed, it begins to contract and usually
expresses most of the fluid from the clot
within 20 to 60 minutes.
Difference from plasma: Serum have no
fibrinogen and most of other clotting factors.
Fibrinolysis
Breakdown of Blood Clots
Fibrinolysis
Fibrinolysis
Fibrinolysis leads to the breakdown of
fibrin clots (blood clots) and is caused by
the action of several enzymes
Blood Groups
Transfusion
Antigenicity causes immune
reactions of blood
Karl Landsteiner
(1868-1943)
ABO blood
type
There are four types: A, B, AB, and O
decided by the antigens (Antigen A/B)
on RBC membrane.
A yes no no yes
B no yes yes no
O no no yes yes
A AA AB AO
(A) (AB) (A)
B AB BB BO
(AB) (B) (B)
O AO BO OO
(A) (B) (O)
The possible ABO alleles for one parent are in the top
row and the alleles of the other are in the left
column. Offspring genotypes are shown in white.
Phenotypes are red.
Titer of the agglutinins at
different ages.
Immediately after birth, antibody
anti-A and anti-B in plasma are
almost zero.
2 to 8 months, infants begin to
produce the certain antibody.
A maximum titer is usually at 8 to 10
years of age.
The antibody (agglutinins) are IgM
(most, spontaneous developed
antibody) and IgG (little, immune
antibody) immunoglobulin
molecules.
AB
O
Differences between ABO system and
Rh system
ABO Rh
Contact Agglutinins Must be massively
history develop exposed to
spontaneously. antigens.
Antigen A, B C, c, D, d, E, e
Blood A, B, O, AB Positive (have D)
type Negative (have no
D)
Antibody IgM (most, can IgG (can go
not go through through placenta)
Rh immune response
Formation of Anti-Rh Agglutinins.
After being exposed to antigen, anti-Rh
agglutinins develop slowly, 2 to 4 months
to get the maximum concentration of
agglutinins.
If an Rh-negative person (never exposed to
Rh-positive blood) take transfusion of Rh-
positive blood, he will have a delayed mild
transfusion reaction.
Repeatedly exposition can make
transfusion reaction very severe.
Hemolytic disease of the
Newborn
In the case that Mother is Rh-
negative and baby is Rh-positive.
An Rh-negative mother having her
first Rh-positive child usually does
not develop sufficient anti-Rh
agglutinins to cause any harm.
The rule of Blood
Transfusion
Blood typing. Avoid mismatch.
ABO type match and Rh type match
(especially in procreation-age woman and
patients needing repeated transfusions).
Cross-match test
1. Main test: Donor’s RBC + Recipient’s serum
2. Subordination test: Donor’s serum + Recipient’s RBC
Advances: transfusion of blood components
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