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Amino Acids
Total amino acids = 20
Why?
Peptide bond
Partial double bond character
Peptide Bond
Glu (E)
pKa = 4.3
Above 4.3 pH it will be vely charged
Lys (K)
pKa = 10.5
Below 10.5 pH it will be +vely charged
pKa values
Acidic amino acids
Asp (D)3.9
Glu (E)4.3
Hydrophobic/Nonpolar
Polar
Hydrophobic/Nonpolar
Biocatalyst
Biochemical reactions
Industrial application: Proteases/Lipases = added detergents
Cellulase = stone wash jeans
Pharmaceutical Industry
pH
pKa
Isoelectric point (pI)
Charge on a polypeptide
Phe 85
Val 6
Leu 88
fibril
formation
at high
concentration
picture of
sickle-cell hemoglobin
fibrils spilling out of
a distorted, ruptured
erythrocyte
Misfolded
protein due to
mutation
(pI)
Histidine
Above pH 6
Below pH 6
Protonated
H+
~6.0
pKa
OH-
Unprotonated
Above pH4.1
Below pH4.1
Protonated
H+
pKa
OH-
Unprotonated
+
Protonated
H+
H+
12.5
pKa
OHOH-
0
Unprotonated
9.9
2.3
4.3
6
12.4
+vely charged
protonation
[H+]
pH
below
pI
pI
deprotonation
[OH-]
-vely charge
pH
above
pI