Professional Documents
Culture Documents
World
for growth
Chief among these is iron (Fe), which
plays a major role in cellular respiration.
Iron is a key component of cytochromes
and of ironsulfur proteins involved in
electron transport reactions .
Under anoxic conditions, iron is
generally in the ferrous form and
soluble. However, under oxic conditions,
the exact
composition of a medium is not essential. In
these instances
complex media may suffice and may even be
advantageous.
Complex media employ digests of microbial,
animal or plant products, such as casein (milk
protein), beef (beef extract), soybeans (tryptic soy
broth), yeast cells (yeast extract), or any of a
number of other highly nutritious yet impure
substances.
liquid media
except that before sterilization, agar, a gelling
agent, is added to the medium, typically at a
concentration of 12%.
The agar melts during the sterilization process,
and the molten medium is then poured into sterile
glass or plastic plates and allowed to solidify
before use.
OBJECTIVES:
By the end of the lesson, student should be able
to :
1. define terms enzyme;
2. understand the Lock & Key model and
Induced-fit model;
3. identify the groups of enzymes; and
4. understand some factors affecting the
enzyme activities.
ENZYME AS BIOLOGICAL
CATALYSTS:
Enzymes are biological catalysts produced by
living cells.
Enzymes lower the amount of activation energy
needed.
They speed up the rate of biochemical reactions
in the cell but remain unchanged at the end of the
reactions.
Most enzymes are globular protein molecules.
substrate.
The enzyme combines with its substrate to form an
enzyme-substrate complex.
The complex than breaks up into product and enzyme.
A metabolic pathway is a number of reactions catalysed
by sequence of enzymes.
MECHANISM ACTION:
close to 7 (neutral).
High or low pH levels usually slow enzyme
activity
METABOLISM
Metabolism is the sum of all biochemical
2) CATABOLISM
of complex molecules
from simpler molecules
which requires energy
input.
Involves the
breakdown of complex
molecules into simpler
molecules involving
hydrolysis or oxidation
and the release of
energy.
energy is represented by a negative number (G), indicating free energy is released during
the reaction.
Vo = Vmax
KM
Vo = Initial reaction velocity
cofactor.
If the cofactor is organic, then it is called a
coenzyme.
Coenzymes are relatively small molecules
compared to the protein part of the enzyme.
Many of the coenzymes are derived from vitamins.
The coenzymes make up a part of the active site,
since without the coenzyme, the enzyme will not
function.
Vitamin
Coenzyme
Function
niacin
nicotinamide adenine
dinucleotide (NAD+)
oxidation or
hydrogen transfer
riboflavin
flavin adenine
dinucleotide (FAD)
oxidation or
hydrogen transfer
pantothenic
acid
coenzyme A (CoA)
vitamin B-12
coenzyme B-12
Methyl group
transfer
thiamin (B-1)
thiaminpyrophosphate
(TPP)
Aldehyde group
transfer
animals.
The variety found in humans has a 10-unit side chain
in its molecular structure.
Apart from the important process that provides
energy, CoQ10 also stabilizes cell membranes and
acts as an antioxidant.
In this capacity, it destroys free radicals, which are
unstable molecules that can damage normal cells.
same way.
Non-specific methods of inhibition include any
physical or chemical changes which ultimately
denatures the protein portion of the enzyme and
are therefore irreversible.
1.Oxidoreductases
These enzymes catalyse oxidation and reduction
oxidases
catalyse hydrogen transfer from the substrate to
dehydrogenases
catalyse hydrogen transfer from the substrate to a
peroxidases
catalyse oxidation of a substrate by hydrogen peroxide.
An example of this type of enzyme is horseradish
oxygenases
catalyse substrate oxidation by molecular oxygen.
The reduced product of the reaction in this case is
2.Transferases
These enzymes transfer C, N, P or S containing
3.Hydrolases
These enzymes catalyse cleavage reactions or the
4.Lyases
These enzymes non-hydrolytically remove groups
5.Isomerases
These enzymes catalyse intramolecular
racemases
epimerases
mutases
cis-trans-isomerases
6.Ligases
Ligases split C-C, C-O, C-N, C-S and C-
catabolism?
What is a substrate?
List 6 types of enzyme and state the
characteristics each of them.