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Lente
LASER
Abertura
PMT
Autocorrelacin
digital y
microcomputadora
Video Display
Brownian
motion
Particles move or diffuse in random walk
fashion
Collision of neighboring solvent molecules
It Probes density or conc. fluctuations
The fluctuation in scattering intensity of
laser due to the Brownian motion of
particles in liquid can be recorded and
particle size can be calculated using
Stokes-Einstein relation.
+ +
- +
++
+
+
+ +
- +
++
+
+
Effect of Diffusion
Intensity Vs time plot
Pequeo
Ia (t)
Mediano
Largo
Tiempo, t
0.8
0.6
0.4
0.2
Diameter (nm)
500
376.95
284.19
214.25
161.53
121.78
91.81
69.22
52.18
39.34
29.66
22.36
16.86
12.71
9.58
7.22
5.45
4.11
3.1
2.33
1.76
1.33
0
1
T = Temperature
= viscosity of solvent
R = particle radius
Step 3. obtaining autocorrelation function
1.2
800000
700000
C(t')
600000
500000
400000
300000
200000
-6
14
24
34
# of channel
44
54
64
T = Temperature
= viscosity of solvent
R = particle radius
Step 3. obtaining autocorrelation function
1/ = 2DK2
Or D = (1/2K2)*(1/ )
decay
t
Channel width
Challenges
Challenges in collecting data
- Sample preparation: sonication
- Sample purification: dust is big enemy
- Right use of cuvette
Challenges in interpreting data
Intensity weight
Volume weight
Number weight
Distribution of particle size manipulations depends on: x axis scale bar
Advantages
disadvantages
Less expensive
Paint pigments
Experimental data
interpretations
No TBAP run 102 before EC
Autocorrelation function
800000
25 nM DSNPs in MeOH
700000
600000
500000
C(t')
0.8
0.6
400000
300000
200000
0.4
100000
0.2
0
-6
14
24
34
# of channel
Diameter (nm)
500
376.95
284.19
214.25
161.53
121.78
91.81
69.22
52.18
39.34
29.66
22.36
16.86
12.71
9.58
7.22
5.45
4.11
3.1
2.33
1.76
1.33
0
1
1.2
44
54
64
References:
PSS Nicomp ZLS 380 manual
Experimental Data obtained using PSS Nicomp ZLS 380 in Dr. Alpuche
lab, UNR.
Thank You
C(0) = <Is2(t)>
C() = <Is (t)>2
<Is2(t)> > <Is (t)>2
Now the function C(t) for diffusing particles must fall from the value
<Is2(t)> at t=0 to the baseline value <Is (t)>2 at
very large t
Ideal case of uniform particle size: exponential